##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552953_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 664272 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.394851807693232 31.0 31.0 34.0 30.0 34.0 2 31.587152551966664 31.0 31.0 34.0 30.0 34.0 3 31.759685791362575 31.0 31.0 34.0 30.0 34.0 4 35.571132006166145 37.0 35.0 37.0 33.0 37.0 5 35.168090782089266 37.0 35.0 37.0 32.0 37.0 6 35.19021424958451 37.0 35.0 37.0 32.0 37.0 7 35.12925879760098 37.0 35.0 37.0 32.0 37.0 8 35.46526874533324 37.0 35.0 37.0 33.0 37.0 9 36.36339933039478 39.0 37.0 39.0 32.0 39.0 10 36.06776892598213 38.0 35.0 39.0 31.0 39.0 11 36.327790423200135 38.0 35.0 39.0 32.0 39.0 12 35.883764482019416 37.0 35.0 39.0 31.0 39.0 13 35.63501095936604 37.0 35.0 39.0 30.0 39.0 14 36.64409308235181 38.0 35.0 40.0 31.0 41.0 15 36.72267534985669 38.0 35.0 40.0 31.0 41.0 16 36.72131747236072 38.0 35.0 40.0 31.0 41.0 17 36.693246140135365 38.0 35.0 40.0 31.0 41.0 18 36.510331611147244 38.0 35.0 40.0 31.0 41.0 19 36.51547558831322 38.0 35.0 40.0 31.0 41.0 20 36.50498139316424 38.0 35.0 40.0 31.0 41.0 21 36.560418021533344 38.0 35.0 40.0 31.0 41.0 22 36.53212388900932 38.0 35.0 40.0 31.0 41.0 23 36.49650594937014 38.0 35.0 40.0 31.0 41.0 24 36.13578925500397 38.0 35.0 40.0 30.0 41.0 25 36.16683226148325 38.0 35.0 40.0 30.0 41.0 26 36.02451104366886 38.0 35.0 40.0 30.0 41.0 27 35.969221945227254 38.0 34.0 40.0 30.0 41.0 28 35.83416732904593 38.0 34.0 40.0 30.0 41.0 29 35.60994592576535 38.0 34.0 40.0 29.0 41.0 30 35.618367174892214 38.0 34.0 40.0 29.0 41.0 31 35.40033299612207 37.0 34.0 40.0 28.0 41.0 32 35.394663631765305 37.0 34.0 40.0 29.0 41.0 33 35.35374364718067 37.0 34.0 40.0 28.0 41.0 34 35.14219175277597 37.0 34.0 40.0 27.0 41.0 35 35.071835031432904 37.0 34.0 40.0 27.0 41.0 36 34.79492737914589 37.0 34.0 40.0 26.0 41.0 37 34.744740106462416 37.0 33.0 40.0 26.0 41.0 38 34.73047185490281 37.0 33.0 40.0 26.0 41.0 39 34.65630645277838 37.0 33.0 40.0 26.0 41.0 40 34.531639147818964 37.0 33.0 40.0 25.0 41.0 41 34.54780571813956 36.0 33.0 40.0 25.0 41.0 42 34.51082839559698 36.0 33.0 40.0 25.0 41.0 43 34.361699725413686 36.0 33.0 40.0 24.0 41.0 44 34.23644230074427 36.0 33.0 40.0 24.0 41.0 45 34.02453513018764 36.0 33.0 39.0 24.0 41.0 46 33.96072994195149 35.0 33.0 39.0 23.0 41.0 47 33.86112164896308 35.0 33.0 39.0 23.0 41.0 48 33.8310601681239 35.0 33.0 39.0 23.0 40.0 49 33.644844280656116 35.0 33.0 39.0 23.0 40.0 50 33.3918665847725 35.0 32.0 39.0 23.0 40.0 51 31.32583339354963 34.0 29.0 38.0 15.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 3.0 10 9.0 11 19.0 12 16.0 13 24.0 14 38.0 15 69.0 16 133.0 17 307.0 18 547.0 19 1010.0 20 1716.0 21 2594.0 22 3571.0 23 4639.0 24 5593.0 25 6605.0 26 7712.0 27 9184.0 28 11639.0 29 14426.0 30 18923.0 31 24245.0 32 31835.0 33 43800.0 34 70286.0 35 76493.0 36 68317.0 37 87487.0 38 103142.0 39 69886.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.222806320302524 35.74093142568104 20.641694968326227 25.3945672856902 2 21.60530626008623 35.11031625599152 20.202417082159116 23.081960401763133 3 19.70262181756871 29.975672616036803 24.927288821446638 25.39441674494785 4 22.570121877785006 32.146921742900496 19.51760724522485 25.76534913408965 5 16.911746995206784 37.13734735168726 18.720795095984776 27.23011055712118 6 21.093768817592792 35.54327143098008 21.70195341667269 21.661006334754436 7 76.7699075077679 10.239480212924827 7.209546691716646 5.781065587590625 8 79.37757424669412 5.809818869378809 5.426993761591637 9.385613122335428 9 74.42162246790471 9.95254955801238 9.799449623045982 5.826378351036925 10 44.51113399330395 27.503191463737746 12.31498542765614 15.670689115302167 11 36.06399185875666 24.36276103764723 20.70582532456584 18.867421779030277 12 32.68811571163619 23.005937326878147 27.32013392104439 16.985813040441265 13 24.693348507840163 32.03296240094419 21.421345472938796 21.85234361827685 14 18.4007454777561 32.07255461618133 30.489769251150133 19.036930654912446 15 17.1072994195149 27.615645398270587 31.611749403858656 23.665305778355854 16 17.97591950285425 24.803694871980152 33.28636462172122 23.93402100344437 17 17.477328564202615 25.607732976852855 27.246670038779296 29.668268420165234 18 23.342395886022594 25.391405930100923 25.62609894741913 25.640099236457353 19 23.587175133078016 29.484458173760146 26.055742226076067 20.87262446708577 20 26.34312450321555 28.796185899751908 23.558421851289832 21.30226774574271 21 22.132801021268396 28.129290411156877 24.456096297902064 25.281812269672667 22 20.542187537635183 27.947286653659948 24.407772719608836 27.102753089096034 23 23.654165763422213 27.49912686369439 20.253751475299275 28.592955897584122 24 20.662469590770048 30.68773032733579 25.92492232097695 22.724877760917213 25 20.63431847195125 26.625689476599945 25.685562540646 27.0544295108028 26 23.4087843533974 32.670502444781654 21.371365946479756 22.549347255341186 27 19.140352144904497 28.53830960811234 26.227810294578124 26.093527952405037 28 22.10028422092155 30.121396054628224 26.60792566900306 21.17039405544717 29 20.457282558951757 31.705235204855843 26.264843317195364 21.572638918997036 30 23.921074499602575 27.974534528024662 24.829136257436712 23.27525471493605 31 29.302755497747913 31.323162800780402 17.586018980176796 21.78806272129489 32 26.438868415347933 29.449984343762797 20.34889322446227 23.762254016427008 33 26.83087650841824 28.231055952983112 20.374033528434136 24.564034010164512 34 21.578811089433245 32.20277235831105 20.93434617144784 25.28407038080786 35 24.638852759110726 29.375316135558926 24.480182816677505 21.50564828865284 36 27.01453621408098 29.824981332947946 24.46482766095816 18.69565479201291 37 23.26065226292844 31.305549533925863 22.809782739600646 22.624015463545057 38 27.00896620661416 28.464243562877854 21.86227930727172 22.664510923236264 39 23.379428908639834 28.97246910903967 24.20273622853289 23.445365753787605 40 25.497988775682252 26.674163595635527 21.160608907194643 26.667238721487585 41 21.24822361924031 24.838469783462198 27.110731748440397 26.8025748488571 42 28.378284798998 24.030969241515525 22.23050196305128 25.360243996435194 43 23.807115157646265 22.904472866536597 26.602205120793894 26.686206855023244 44 22.990431630416456 24.151251294650383 24.942041814196596 27.91627526073657 45 26.583839150227618 27.44809355203892 23.51973288050678 22.44833441722668 46 21.863784714695186 24.92277259917624 29.733904183828315 23.47953850230026 47 23.20329624009442 25.8729857648674 23.585067562685165 27.338650432353013 48 22.945269407712505 21.918882626394005 31.349055808464005 23.78679215742949 49 24.40972974925934 21.24340631548522 28.418780258689214 25.928083676566228 50 21.409452754293422 23.540658043692943 24.475064431437723 30.57482477057591 51 20.683996916925594 24.263404147698534 28.711883084037865 26.340715851338004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2761.0 1 2333.0 2 1905.0 3 1450.0 4 995.0 5 935.0 6 875.0 7 949.5 8 1024.0 9 1055.5 10 1087.0 11 1242.0 12 1397.0 13 1463.0 14 1529.0 15 1674.0 16 1819.0 17 1767.0 18 1715.0 19 1641.0 20 1567.0 21 1618.5 22 1670.0 23 1992.0 24 2314.0 25 2079.5 26 2377.0 27 2909.0 28 3465.0 29 4021.0 30 4520.5 31 5020.0 32 6084.5 33 7149.0 34 7513.0 35 7877.0 36 9491.5 37 11106.0 38 12476.0 39 13846.0 40 20440.0 41 27034.0 42 44041.0 43 61048.0 44 66868.0 45 72688.0 46 71945.5 47 71203.0 48 64743.0 49 58283.0 50 53794.5 51 49306.0 52 45101.5 53 40897.0 54 36877.5 55 32858.0 56 31107.0 57 29356.0 58 28390.5 59 27425.0 60 27314.0 61 27203.0 62 26179.5 63 25156.0 64 23028.0 65 20900.0 66 16985.5 67 13071.0 68 12167.0 69 11263.0 70 9380.5 71 7498.0 72 6584.0 73 5670.0 74 4944.5 75 3228.0 76 2237.0 77 1730.5 78 1224.0 79 939.5 80 655.0 81 485.5 82 316.0 83 240.0 84 164.0 85 116.0 86 68.0 87 45.5 88 23.0 89 23.5 90 24.0 91 28.0 92 32.0 93 22.5 94 13.0 95 7.5 96 2.0 97 2.0 98 2.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 664272.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.220305308375213 #Duplication Level Percentage of deduplicated Percentage of total 1 78.69349271073082 21.420608973684924 2 9.514702698007223 5.17986224716356 3 3.5319957632716474 2.8842600907242595 4 1.660068641653535 1.807503010346758 5 0.9018507631343301 1.227432655755382 6 0.5777962203032428 0.9436673713607698 7 0.40658336713854365 0.7747126370772858 8 0.30275145006633286 0.6592789522687116 9 0.23155003417533446 0.5672576361975588 >10 2.497690195058949 16.78370651318745 >50 1.176234375454781 23.396945924277805 >100 0.49479154948708143 17.553834849061275 >500 0.004970004403330059 0.9127230512634983 >1k 0.003313336268886706 1.3776526292539464 >5k 0.0022088908459244705 4.510553458376778 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC 8460 1.2735746802514631 No Hit AATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT 7867 1.1843040200399837 No Hit AATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT 7253 1.0918720042392274 No Hit CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT 6427 0.9675253510610111 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2351 0.35392128525664185 No Hit AATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT 1666 0.2508008767492834 No Hit AAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT 1515 0.22806922465496063 No Hit AATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT 1333 0.20067080954789604 No Hit AACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG 1169 0.17598212780306863 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 1131 0.1702615795939013 No Hit AATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC 980 0.1475299274995785 No Hit AAAAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT 888 0.13368017920369968 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.5054074234650865E-4 0.0 0.0 1.2893814581978467 0.0 2 1.5054074234650865E-4 0.0 0.0 1.5746561649444806 0.0 3 1.5054074234650865E-4 0.0 0.0 2.121119059662307 0.0 4 1.5054074234650865E-4 0.0 0.0 5.0547065057687215 0.0 5 1.5054074234650865E-4 0.0 0.0 5.468392465736927 0.0 6 1.5054074234650865E-4 0.0 0.0 7.05027458631404 0.0 7 1.5054074234650865E-4 0.0 0.0 8.272966495652383 0.0 8 1.5054074234650865E-4 0.0 0.0 8.983819881012597 0.0 9 1.5054074234650865E-4 0.0 0.0 11.275953223980538 0.0 10 1.5054074234650865E-4 0.0 0.0 12.820350699713371 0.0 11 1.5054074234650865E-4 0.0 0.0 15.875575065635763 0.0 12 1.5054074234650865E-4 0.0 0.0 16.742087578582268 0.0 13 1.5054074234650865E-4 0.0 0.0 17.204699279813088 0.0 14 1.5054074234650865E-4 0.0 0.0 17.679354240431632 0.0 15 1.5054074234650865E-4 0.0 0.0 17.97426355468844 0.0 16 1.5054074234650865E-4 0.0 0.0 18.51365103451598 0.0 17 1.5054074234650865E-4 0.0 0.0 19.112050485343353 0.0 18 1.5054074234650865E-4 0.0 0.0 19.959594864754198 0.0 19 1.5054074234650865E-4 0.0 0.0 20.34106510586025 0.0 20 1.5054074234650865E-4 0.0 0.0 20.688663679938337 0.0 21 1.5054074234650865E-4 0.0 0.0 21.064714454319915 0.0 22 1.5054074234650865E-4 0.0 0.0 21.464249584507552 0.0 23 1.5054074234650865E-4 0.0 0.0 21.86619336657273 0.0 24 1.5054074234650865E-4 0.0 0.0 22.16155430305658 0.0 25 3.010814846930173E-4 0.0 0.0 22.4021184093263 0.0 26 3.010814846930173E-4 0.0 0.0 22.622660596863934 0.0 27 3.010814846930173E-4 0.0 0.0 22.86774092540405 0.0 28 3.010814846930173E-4 0.0 0.0 23.109509357612545 0.0 29 3.010814846930173E-4 0.0 0.0 23.38183756051738 0.0 30 3.010814846930173E-4 0.0 0.0 23.728231808656695 0.0 31 3.010814846930173E-4 0.0 0.0 23.990022159597274 0.0 32 3.010814846930173E-4 0.0 0.0 24.23856492521136 0.0 33 3.010814846930173E-4 0.0 0.0 24.50547366139172 0.0 34 3.010814846930173E-4 0.0 0.0 24.748747501023676 0.0 35 3.010814846930173E-4 0.0 0.0 25.037334104101934 0.0 36 3.010814846930173E-4 0.0 0.0 25.280005780764505 0.0 37 4.5162222703952595E-4 0.0 0.0 25.517559072187296 0.0 38 4.5162222703952595E-4 0.0 0.0 25.774532119372786 0.0 39 4.5162222703952595E-4 0.0 0.0 26.02713948503023 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTTACG 20 7.0311327E-4 45.000004 1 AGCGTTC 20 7.0311327E-4 45.000004 36 ACTATCG 20 7.0311327E-4 45.000004 12 GTCGACT 20 7.0311327E-4 45.000004 11 TACCAGT 20 7.0311327E-4 45.000004 43 ACGCATT 20 7.0311327E-4 45.000004 23 ACGCATC 20 7.0311327E-4 45.000004 11 CGACGAA 20 7.0311327E-4 45.000004 18 GCGCGTT 20 7.0311327E-4 45.000004 41 CGGTTGC 20 7.0311327E-4 45.000004 38 TAGAACG 20 7.0311327E-4 45.000004 1 GAATCTA 20 7.0311327E-4 45.000004 9 CCAATAC 20 7.0311327E-4 45.000004 31 CACGCAT 20 7.0311327E-4 45.000004 32 CAACCCA 20 7.0311327E-4 45.000004 24 ATGCTAT 20 7.0311327E-4 45.000004 10 CGTCTAA 20 7.0311327E-4 45.000004 1 CACGATT 20 7.0311327E-4 45.000004 16 TTCCGAA 20 7.0311327E-4 45.000004 33 TAACGCG 20 7.0311327E-4 45.000004 1 >>END_MODULE