FastQCFastQC Report
Sat 18 Jun 2016
SRR3552952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681967
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC80751.1840748892541721No Hit
AATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT71951.0550363873911788No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT69781.0232166659090542No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT62220.9123608620358462No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24510.35940155462067813No Hit
AATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT15340.22493757029299072No Hit
AAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT13400.196490446018649No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT12280.18006736396335893No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG11290.1655505325037722No Hit
AACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG10750.15763226079854303No Hit
AATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC9230.1353437922949351No Hit
AAAAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT9190.1347572536501033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCC302.1641972E-645.00000423
GTCGACA302.1641972E-645.00000426
ACGCATT302.1641972E-645.00000423
AACCCGC302.1641972E-645.00000445
TAAACGG900.045.0000042
ACCGAAC302.1641972E-645.00000421
CGATTTA302.1641972E-645.00000422
ATCACCC302.1641972E-645.00000445
ATCGTAG302.1641972E-645.0000041
ATATCAT302.1641972E-645.00000437
GTCACTA302.1641972E-645.00000442
ACTAACT302.1641972E-645.00000417
TCACTCG302.1641972E-645.00000418
CAATGCG302.1641972E-645.0000041
CGCGTCA302.1641972E-645.00000439
GCTAACG302.1641972E-645.0000041
GTTATCG302.1641972E-645.0000042
TACGTTC302.1641972E-645.0000042
TAGTAGT302.1641972E-645.00000432
GCTCACG453.8380676E-1045.0000041