##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552952_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 681967 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.193666262443784 31.0 31.0 34.0 28.0 34.0 2 31.423354795759913 31.0 31.0 34.0 30.0 34.0 3 31.384283990281055 31.0 31.0 34.0 28.0 34.0 4 35.37523516533791 37.0 35.0 37.0 33.0 37.0 5 35.040790830054824 37.0 35.0 37.0 32.0 37.0 6 35.102814359052566 36.0 35.0 37.0 32.0 37.0 7 35.00281831818842 37.0 35.0 37.0 32.0 37.0 8 35.38852906372303 37.0 35.0 37.0 33.0 37.0 9 35.88524664683189 39.0 37.0 39.0 32.0 39.0 10 35.73959297150742 38.0 35.0 39.0 30.0 39.0 11 36.15759706847985 38.0 35.0 39.0 32.0 39.0 12 35.760344708761565 37.0 35.0 39.0 31.0 39.0 13 35.492956404048876 37.0 35.0 39.0 30.0 39.0 14 36.499802776380676 38.0 35.0 40.0 31.0 41.0 15 36.6265875035009 38.0 35.0 40.0 31.0 41.0 16 36.628046518379925 38.0 35.0 40.0 31.0 41.0 17 36.61207800377438 38.0 35.0 40.0 31.0 41.0 18 36.456837354300134 38.0 35.0 40.0 31.0 41.0 19 36.457212739032826 38.0 35.0 40.0 31.0 41.0 20 36.42493991644757 38.0 35.0 40.0 31.0 41.0 21 36.451345886237895 38.0 35.0 40.0 31.0 41.0 22 36.44680461078029 38.0 35.0 40.0 31.0 41.0 23 36.41145392665628 38.0 35.0 40.0 31.0 41.0 24 36.0148584902202 38.0 34.0 40.0 30.0 41.0 25 36.09874964624388 38.0 35.0 40.0 30.0 41.0 26 35.95450806270685 38.0 34.0 40.0 30.0 41.0 27 35.88792419574554 38.0 34.0 40.0 30.0 41.0 28 35.73150900263502 38.0 34.0 40.0 29.0 41.0 29 35.50197150301994 37.0 34.0 40.0 29.0 41.0 30 35.54894591673791 37.0 34.0 40.0 29.0 41.0 31 35.41498782199139 37.0 34.0 40.0 29.0 41.0 32 35.376286535858775 37.0 34.0 40.0 29.0 41.0 33 35.289187013447865 37.0 34.0 40.0 28.0 41.0 34 35.162902310522355 37.0 34.0 40.0 27.0 41.0 35 35.045764677763 37.0 34.0 40.0 27.0 41.0 36 34.826701291998 37.0 34.0 40.0 26.0 41.0 37 34.74891600326702 37.0 33.0 40.0 26.0 41.0 38 34.75198066768627 37.0 33.0 40.0 26.0 41.0 39 34.60390165506542 37.0 33.0 40.0 26.0 41.0 40 34.44603331246233 36.0 33.0 40.0 25.0 41.0 41 34.470068199780926 36.0 33.0 40.0 25.0 41.0 42 34.481348804267654 36.0 33.0 40.0 25.0 41.0 43 34.3528132592926 36.0 33.0 40.0 25.0 41.0 44 34.33550597023023 36.0 33.0 40.0 25.0 41.0 45 34.149794638156976 36.0 33.0 39.0 24.0 41.0 46 33.98830002038222 35.0 33.0 39.0 24.0 41.0 47 33.86551402047313 35.0 33.0 39.0 23.0 40.0 48 33.77462399207 35.0 33.0 39.0 23.0 40.0 49 33.609923940601234 35.0 33.0 39.0 23.0 40.0 50 33.47877243327023 35.0 32.0 39.0 23.0 40.0 51 31.545060098215895 34.0 29.0 38.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 3.0 9 4.0 10 8.0 11 10.0 12 12.0 13 24.0 14 37.0 15 83.0 16 130.0 17 279.0 18 611.0 19 1097.0 20 1784.0 21 2744.0 22 3784.0 23 4710.0 24 5716.0 25 6699.0 26 7960.0 27 9599.0 28 12065.0 29 15464.0 30 19448.0 31 25783.0 32 33977.0 33 46066.0 34 74179.0 35 78894.0 36 71845.0 37 91659.0 38 102407.0 39 64881.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.279770135505093 35.306253821665855 20.852621901059727 25.56135414176932 2 21.751052470280822 34.69728007366925 20.2838260502341 23.267841405815822 3 20.13484523444683 29.936345893569634 24.487108613759904 25.441700258223637 4 22.551091181831378 32.36740194173618 19.355042106142967 25.72646477028947 5 17.01929858776158 37.14974478237217 18.513505785470556 27.317450844395697 6 21.035475323586038 35.51931398440101 21.592687036176237 21.85252365583672 7 77.38131023935175 10.164274810951262 6.798716066906463 5.655698882790516 8 80.03701058848888 5.869785488154119 5.188374217520789 8.904829705836207 9 74.30608812449869 10.894808693089256 9.206163934618537 5.592939247793515 10 45.1491054552493 27.557345150131894 12.036946069237954 15.256603325380846 11 36.63989606535213 24.504998042427275 20.118715421713954 18.736390470506635 12 33.28636136352639 23.224437546098272 26.646743904030544 16.842457186344795 13 24.607788939934043 32.361683189949076 21.248095582337562 21.782432287779322 14 18.58843609734782 32.298043746984824 29.928280987203195 19.185239168464165 15 17.36315686829421 27.286217661558403 31.902423431045783 23.4482020391016 16 18.242671566219478 24.844897187107293 33.23137336557341 23.681057881099818 17 17.889721936691952 25.389058414849984 27.026967580542756 29.69425206791531 18 23.697041059171482 25.357825232012694 25.58070991704877 25.36442379176705 19 23.995882498713282 29.29496588544607 25.72367871172652 20.98547290411413 20 26.801590106266136 28.411198782345775 23.378403940366617 21.408807171021472 21 22.35240121589461 28.071153003004547 24.539897091794767 25.03654868930608 22 20.890013739667758 27.776857237960193 24.173603708097314 27.159525314274735 23 23.691322307384375 27.65910960501021 20.38251117722705 28.267056910378365 24 20.873150753628842 30.305132066507618 26.19730866742819 22.624408512435352 25 20.62753769610553 26.687361705185147 25.85506336816884 26.830037230540484 26 23.28470439185474 32.727243400340484 21.585648572438256 22.40240363536652 27 19.310025265152124 28.81840323652024 26.178539430793574 25.693032067534062 28 22.125410760344707 30.137088744763307 26.72797950633975 21.009520988552232 29 20.581494412486233 31.48422137728072 26.324587553356686 21.60969665687636 30 23.95101229238365 28.13141984876101 24.90648374481463 23.01108411404071 31 29.35728561645945 31.11015635653925 17.848957500876143 21.683600526125165 32 26.67636410559455 29.231033173159403 20.59469153199495 23.49791118925109 33 26.971832947928565 28.257818926722262 20.43529965526191 24.335048470087262 34 21.70017024284166 32.02647635442771 21.12228304302114 25.151070359709486 35 24.56497161886132 29.421951502052153 24.56233219495958 21.450744684126942 36 27.394580676191076 29.745134295354468 24.196185445923337 18.66409958253112 37 23.240713993492353 31.42072856897768 22.88893744125449 22.44961999627548 38 26.80012375965406 28.734674844970503 21.68814620062261 22.777055194752826 39 23.45304098292146 28.97222299612738 24.133279176265127 23.441456844686034 40 25.58378924493414 26.5628688778196 21.29399223129565 26.559349645950608 41 21.15982151629038 24.997837138747183 26.92505649100323 26.91728485395921 42 28.211775643102964 24.014651735347897 22.217204058260883 25.556368563288252 43 23.901449776895365 23.152879831428795 26.432070760022114 26.513599631653733 44 22.983076893749992 24.527139876269676 25.076140047832222 27.41364318214811 45 26.286462541442624 27.958977487180466 23.45524050283958 22.299319468537334 46 22.140074226465504 25.225414132941914 29.315054834031557 23.31945680656102 47 23.226343796693975 26.054193238089233 23.54806024338421 27.17140272183258 48 23.20816109870419 22.215737711648803 30.94255293877856 23.633548250868444 49 24.488574960371984 21.67157648390611 28.132886195373093 25.70696236034881 50 21.58242260989168 24.13973110135828 24.396195123810976 29.881651164939065 51 20.816989678386197 24.777445242951636 28.272922296826682 26.132642781835486 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2926.0 1 2464.0 2 2002.0 3 1468.5 4 935.0 5 915.5 6 896.0 7 962.0 8 1028.0 9 1092.0 10 1156.0 11 1271.5 12 1387.0 13 1507.5 14 1628.0 15 1729.5 16 1831.0 17 1830.5 18 1830.0 19 1685.5 20 1541.0 21 1637.0 22 1733.0 23 2044.0 24 2355.0 25 2147.5 26 2531.0 27 3122.0 28 3638.0 29 4154.0 30 4746.5 31 5339.0 32 6435.0 33 7531.0 34 7967.5 35 8404.0 36 10022.0 37 11640.0 38 13378.0 39 15116.0 40 21609.0 41 28102.0 42 44486.5 43 60871.0 44 67049.0 45 73227.0 46 72648.0 47 72069.0 48 65559.0 49 59049.0 50 54775.5 51 50502.0 52 46047.5 53 41593.0 54 37692.5 55 33792.0 56 32338.0 57 30884.0 58 29946.5 59 29009.0 60 28572.0 61 28135.0 62 27049.5 63 25964.0 64 23720.5 65 21477.0 66 17536.5 67 13596.0 68 12745.5 69 11895.0 70 9820.0 71 7745.0 72 6902.0 73 6059.0 74 5273.5 75 3492.0 76 2496.0 77 1879.0 78 1262.0 79 950.5 80 639.0 81 473.0 82 307.0 83 220.5 84 134.0 85 95.5 86 57.0 87 49.0 88 41.0 89 38.5 90 36.0 91 30.5 92 25.0 93 17.5 94 10.0 95 8.0 96 6.0 97 3.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 681967.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.37566489841517 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73229074658681 21.55348808163154 2 9.46123403178986 5.180151447595215 3 3.496600467612885 2.8716528805503643 4 1.740161492073038 1.9055231150447054 5 0.9879785758850343 1.3523285210121072 6 0.5919686014853871 0.972332043878846 7 0.41749630844872915 0.8000467325492452 8 0.27115547239579174 0.5938449078142927 9 0.22645648396333395 0.5579457137148203 >10 2.396742873537613 16.089819437004728 >50 1.1282995596022585 22.503994120523885 >100 0.5399257911646296 19.325600420184266 >500 0.0043064868687109036 0.8072719162861782 >1k 0.0032298651515331777 1.2904856098791644 >5k 0.0021532434343554518 4.195515052330685 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC 8075 1.1840748892541721 No Hit AATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT 7195 1.0550363873911788 No Hit AATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT 6978 1.0232166659090542 No Hit CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT 6222 0.9123608620358462 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2451 0.35940155462067813 No Hit AATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT 1534 0.22493757029299072 No Hit AAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT 1340 0.196490446018649 No Hit AATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT 1228 0.18006736396335893 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 1129 0.1655505325037722 No Hit AACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG 1075 0.15763226079854303 No Hit AATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC 923 0.1353437922949351 No Hit AAAAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT 919 0.1347572536501033 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.9326932241589403E-4 0.0 0.0 1.1896470063800741 0.0 2 2.9326932241589403E-4 0.0 0.0 1.4546158391828343 0.0 3 2.9326932241589403E-4 0.0 0.0 1.933964546671613 0.0 4 4.39903983623841E-4 0.0 0.0 4.527051895472948 0.0 5 4.39903983623841E-4 0.0 0.0 4.898184222990262 0.0 6 4.39903983623841E-4 0.0 0.0 6.338576500035925 0.0 7 4.39903983623841E-4 0.0 0.0 7.45534607979565 0.0 8 4.39903983623841E-4 0.0 0.0 8.11036311141155 0.0 9 4.39903983623841E-4 0.0 0.0 10.251082530386368 0.0 10 4.39903983623841E-4 0.0 0.0 11.683116631743179 0.0 11 4.39903983623841E-4 0.0 0.0 14.516684824925546 0.0 12 4.39903983623841E-4 0.0 0.0 15.32786777072791 0.0 13 4.39903983623841E-4 0.0 0.0 15.765278965111214 0.0 14 4.39903983623841E-4 0.0 0.0 16.238908920812882 0.0 15 4.39903983623841E-4 0.0 0.0 16.52557968347442 0.0 16 4.39903983623841E-4 0.0 0.0 17.04495965347297 0.0 17 4.39903983623841E-4 0.0 0.0 17.605837232593366 0.0 18 4.39903983623841E-4 0.0 0.0 18.429484124598403 0.0 19 4.39903983623841E-4 0.0 0.0 18.789178948541498 0.0 20 4.39903983623841E-4 0.0 0.0 19.12673193864219 0.0 21 4.39903983623841E-4 0.0 0.0 19.500210420738835 0.0 22 4.39903983623841E-4 0.0 0.0 19.867970151048365 0.0 23 4.39903983623841E-4 0.0 0.0 20.2524462327356 0.0 24 4.39903983623841E-4 0.0 0.0 20.541463149976465 0.0 25 4.39903983623841E-4 0.0 0.0 20.784290148936826 0.0 26 4.39903983623841E-4 0.0 0.0 20.997936850316805 0.0 27 4.39903983623841E-4 0.0 0.0 21.227273460446032 0.0 28 4.39903983623841E-4 0.0 0.0 21.459542763799423 0.0 29 4.39903983623841E-4 0.0 0.0 21.713807266334 0.0 30 4.39903983623841E-4 0.0 0.0 22.042855446084634 0.0 31 4.39903983623841E-4 0.0 0.0 22.289494946236402 0.0 32 4.39903983623841E-4 0.0 0.0 22.532028675874347 0.0 33 4.39903983623841E-4 0.0 0.0 22.784093658490807 0.0 34 4.39903983623841E-4 0.0 0.0 23.02604084948392 0.0 35 4.39903983623841E-4 0.0 0.0 23.305673148407475 0.0 36 4.39903983623841E-4 0.0 0.0 23.537502547777237 0.0 37 4.39903983623841E-4 0.0 0.0 23.76361319535989 0.0 38 4.39903983623841E-4 0.0 0.0 24.01391856204186 0.0 39 4.39903983623841E-4 0.0 0.0 24.26319748609537 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACCC 30 2.1641972E-6 45.000004 23 GTCGACA 30 2.1641972E-6 45.000004 26 ACGCATT 30 2.1641972E-6 45.000004 23 AACCCGC 30 2.1641972E-6 45.000004 45 TAAACGG 90 0.0 45.000004 2 ACCGAAC 30 2.1641972E-6 45.000004 21 CGATTTA 30 2.1641972E-6 45.000004 22 ATCACCC 30 2.1641972E-6 45.000004 45 ATCGTAG 30 2.1641972E-6 45.000004 1 ATATCAT 30 2.1641972E-6 45.000004 37 GTCACTA 30 2.1641972E-6 45.000004 42 ACTAACT 30 2.1641972E-6 45.000004 17 TCACTCG 30 2.1641972E-6 45.000004 18 CAATGCG 30 2.1641972E-6 45.000004 1 CGCGTCA 30 2.1641972E-6 45.000004 39 GCTAACG 30 2.1641972E-6 45.000004 1 GTTATCG 30 2.1641972E-6 45.000004 2 TACGTTC 30 2.1641972E-6 45.000004 2 TAGTAGT 30 2.1641972E-6 45.000004 32 GCTCACG 45 3.8380676E-10 45.000004 1 >>END_MODULE