##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	877067
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192900884425022	31.0	31.0	34.0	28.0	34.0
2	31.400618196785423	31.0	31.0	34.0	30.0	34.0
3	31.37435224446935	31.0	31.0	34.0	28.0	34.0
4	35.37785824800158	37.0	35.0	37.0	33.0	37.0
5	35.03980425668735	37.0	35.0	37.0	32.0	37.0
6	35.1133687620216	36.0	35.0	37.0	32.0	37.0
7	34.9996397082549	37.0	35.0	37.0	32.0	37.0
8	35.3921114350443	37.0	35.0	37.0	33.0	37.0
9	35.91520716205262	39.0	37.0	39.0	32.0	39.0
10	35.80878883825295	38.0	35.0	39.0	30.0	39.0
11	36.17262649261687	38.0	35.0	39.0	32.0	39.0
12	35.813393959640486	37.0	35.0	39.0	31.0	39.0
13	35.5840237974978	37.0	35.0	39.0	30.0	39.0
14	36.55951141702971	38.0	35.0	40.0	31.0	41.0
15	36.65627369402794	38.0	35.0	40.0	31.0	41.0
16	36.66829900110254	38.0	35.0	40.0	31.0	41.0
17	36.638131408432876	38.0	35.0	40.0	31.0	41.0
18	36.46684346805888	38.0	35.0	40.0	31.0	41.0
19	36.47769896712566	38.0	35.0	40.0	31.0	41.0
20	36.448768452125094	38.0	35.0	40.0	31.0	41.0
21	36.483463635047265	38.0	35.0	40.0	31.0	41.0
22	36.46957986105964	38.0	35.0	40.0	31.0	41.0
23	36.439119246306156	38.0	35.0	40.0	31.0	41.0
24	36.036707571941484	38.0	34.0	40.0	30.0	41.0
25	36.15562209044463	38.0	35.0	40.0	30.0	41.0
26	36.05767176281858	38.0	34.0	40.0	30.0	41.0
27	35.97465073933918	38.0	34.0	40.0	30.0	41.0
28	35.9741684500728	38.0	34.0	40.0	30.0	41.0
29	35.7945983602165	38.0	34.0	40.0	30.0	41.0
30	35.78229941384181	38.0	34.0	40.0	30.0	41.0
31	35.67580697939838	38.0	34.0	40.0	29.0	41.0
32	35.61432934998125	38.0	34.0	40.0	29.0	41.0
33	35.56822340824589	38.0	34.0	40.0	29.0	41.0
34	35.49313564414121	38.0	34.0	40.0	29.0	41.0
35	35.44619966319563	38.0	34.0	40.0	29.0	41.0
36	35.332052169332556	38.0	34.0	40.0	28.0	41.0
37	35.183183268781065	37.0	34.0	40.0	27.0	41.0
38	35.155018943820714	37.0	34.0	40.0	27.0	41.0
39	34.97340340019634	37.0	34.0	40.0	27.0	41.0
40	34.76151992949227	37.0	34.0	40.0	26.0	41.0
41	34.78127212630278	37.0	34.0	40.0	26.0	41.0
42	34.78077045425264	37.0	34.0	40.0	26.0	41.0
43	34.7361307631002	37.0	33.0	40.0	26.0	41.0
44	34.6536421960922	37.0	33.0	40.0	26.0	41.0
45	34.40913864049155	36.0	33.0	40.0	25.0	41.0
46	34.269321499953826	36.0	33.0	40.0	24.0	41.0
47	34.12117660338378	36.0	33.0	40.0	24.0	41.0
48	34.06105120817452	36.0	33.0	39.0	24.0	41.0
49	33.92352123611993	36.0	33.0	39.0	24.0	41.0
50	33.78173161229416	35.0	33.0	39.0	23.0	40.0
51	31.796720204955836	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	10.0
10	17.0
11	21.0
12	9.0
13	26.0
14	45.0
15	98.0
16	173.0
17	359.0
18	808.0
19	1377.0
20	2269.0
21	3364.0
22	4575.0
23	5775.0
24	7073.0
25	8214.0
26	9580.0
27	11557.0
28	14232.0
29	18457.0
30	23796.0
31	31102.0
32	41575.0
33	57292.0
34	91785.0
35	97314.0
36	92181.0
37	119622.0
38	141616.0
39	92736.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.677402068485076	36.26963504498516	19.806012539520925	24.246950347008838
2	20.20028116438083	35.990522958907356	20.82030221180366	22.988893664908154
3	21.456741617231067	30.05779490050361	24.871874098558035	23.613589383707286
4	23.110321104317002	30.290388305568445	19.25987410311869	27.33941648699586
5	16.93222980684486	38.039169185478414	18.715331895966898	26.313269111709825
6	21.05836840287002	34.5370422100022	22.819123282485833	21.58546610464195
7	76.12690934672037	10.763145803000226	7.159544253745723	5.950400596533674
8	79.13295107443331	5.768886527483077	5.676989329207461	9.42117306887615
9	73.05063353198787	11.259687116263638	9.461534865637402	6.228144486111095
10	44.2145240899498	25.974868510615494	13.025572732755878	16.78503466667883
11	34.90577116685498	23.74801468986976	22.901215072508712	18.444999070766542
12	32.69430955673854	22.36043540573297	27.981100645674733	16.964154391853757
13	24.65159446199663	31.525185647162644	22.652545358564396	21.170674532276326
14	18.950433661282435	31.545480561918303	30.68693725792898	18.81714851887028
15	16.56999978336889	27.697997986470817	32.138707761208664	23.59329446895163
16	18.531537499415666	24.138064708853484	34.07778425137418	23.252613540356666
17	17.771732376203868	25.898135490219104	27.825810342881447	28.50432179069558
18	20.53959389647541	26.894182542496754	26.9865358062725	25.579687754755337
19	22.742846327589568	30.127002840147902	26.906268278250124	20.223882554012405
20	25.976122690740844	30.13464193727503	23.2893268131169	20.599908558867224
21	21.490490464240473	29.45510434208561	24.965823591584225	24.088581602089693
22	20.773783530790695	28.988435319080523	24.56334578772203	25.67443536240675
23	23.1300459371975	26.827710995853227	22.217116822318022	27.825126244631253
24	19.196823047726113	31.092265471166968	26.14976962991425	23.56114185119267
25	19.42246145391401	25.60534143913749	28.46658237056006	26.505614736388438
26	21.302933527313193	32.273019050996105	23.671167653098337	22.752879768592365
27	18.135444612555254	28.374115090409287	26.763975842210456	26.726464454825
28	20.759189434786624	26.00097826049777	31.141178496055606	22.09865380866
29	19.53123307569433	26.940701223509723	29.50447343247437	24.023592268321575
30	21.944275636866966	25.604429308137235	26.93112384800705	25.520171206988746
31	26.212250603431663	27.36438607312782	24.924435647447687	21.498927675992828
32	24.832196400046975	24.569958737473875	21.93675055611487	28.661094306364284
33	25.273553787795006	24.47258875319673	27.68488610334216	22.5689713556661
34	21.01230578735718	24.494593913577866	27.053235385666092	27.439864913398864
35	20.90433228020208	22.097513644909682	29.53480178823283	27.463352286655407
36	24.15801757448405	23.40243105714843	31.746833480224428	20.692717888143093
37	20.74607755165797	27.29164362585755	23.671167653098337	28.291111169386145
38	22.24060419557457	24.434735316686183	30.099638910140275	23.225021577598977
39	23.467648423666606	24.09496651909147	27.392434101385643	25.044950955856283
40	23.40847392502511	23.780395340378785	25.431922532714147	27.379208201881955
41	22.39794679311843	20.463544974329213	30.574859161272745	26.563649071279617
42	24.028723005198007	22.220081248068848	24.337479348784072	29.413716397949074
43	21.890459907851966	22.06228258502486	27.844395012011624	28.202862495111546
44	21.49516513561678	22.36978474848558	26.718597324947808	29.416452790949837
45	24.352871559413362	25.779444443811023	25.95206523560914	23.915618761166478
46	20.153420434242765	23.638672986214278	32.77754151051174	23.430365069031215
47	22.67398043707037	24.30578279652524	26.06596759426589	26.954269172138503
48	22.288947138588043	19.740453123877653	33.530277618471565	24.440322119062742
49	22.78947902497757	19.974756774567965	29.83249854344081	27.40326565701366
50	20.85279687868772	22.342648851228013	26.717001095697363	30.087553174386905
51	19.8127395056478	23.010556776164194	30.850208706974495	26.326495011213513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3502.0
1	3013.0
2	2524.0
3	1864.0
4	1204.0
5	1156.5
6	1109.0
7	1128.0
8	1147.0
9	1264.0
10	1381.0
11	1531.0
12	1681.0
13	1805.0
14	1929.0
15	2183.0
16	2437.0
17	2460.5
18	2484.0
19	2642.0
20	2800.0
21	2787.5
22	2775.0
23	3089.0
24	3403.0
25	3474.5
26	4172.5
27	4799.0
28	5676.0
29	6553.0
30	8329.0
31	10105.0
32	11688.0
33	13271.0
34	15303.0
35	17335.0
36	17787.0
37	18239.0
38	21088.5
39	23938.0
40	28489.0
41	33040.0
42	41858.5
43	50677.0
44	71364.5
45	92052.0
46	92384.0
47	92716.0
48	89519.5
49	86323.0
50	79746.5
51	73170.0
52	65922.0
53	58674.0
54	52628.5
55	46583.0
56	42621.5
57	38660.0
58	38867.5
59	39075.0
60	36917.5
61	34760.0
62	31539.0
63	28318.0
64	25430.0
65	22542.0
66	19269.5
67	15997.0
68	14252.5
69	12508.0
70	10992.0
71	9476.0
72	7600.5
73	5725.0
74	4753.5
75	3367.0
76	2952.0
77	2178.0
78	1404.0
79	1450.5
80	1497.0
81	995.0
82	493.0
83	324.5
84	156.0
85	138.5
86	121.0
87	100.5
88	80.0
89	79.0
90	78.0
91	55.0
92	32.0
93	21.5
94	11.0
95	6.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	877067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.751181203011704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98554638905324	19.82468755077373
2	10.119328182376846	5.211693073542584
3	3.9267919705761356	3.0335859474251237
4	1.9527479632576616	2.0114226658264034
5	1.10376772162343	1.4211661302780165
6	0.7138201823284434	1.1029027716903956
7	0.47452989320661815	0.8553793686346589
8	0.3188712659845813	0.6569049400642156
9	0.2600966699833807	0.6028016831137772
>10	2.418146215818205	14.985434765714889
>50	1.063533296100699	20.01796648290062
>100	0.6512697627657889	22.97416378116935
>500	0.0062194929595641504	1.0290234394874034
>1k	0.0035539959768938	1.5497699315993165
>5k	8.8849899422345E-4	2.123025790995801
>10k+	8.8849899422345E-4	2.6000716767837355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC	11833	1.349155765751077	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGT	10895	1.2422084059712657	No Hit
AATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	9917	1.1307003911901827	No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	8641	0.9852154966496287	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2844	0.3242625705903882	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	2409	0.27466544745156296	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT	1787	0.20374726218179454	No Hit
AAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	1734	0.1977043943051101	TruSeq Adapter, Index 23 (95% over 22bp)
AACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	1356	0.15460620454309648	TruSeq Adapter, Index 23 (95% over 23bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTAT	1178	0.13431128978743928	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	1138	0.129750634786168	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1101	0.12553202890999204	No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	914	0.10421096677904881	TruSeq Adapter, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2803275006356413E-4	0.0	0.0	1.2570305347253972	0.0
2	2.2803275006356413E-4	0.0	0.0	1.5530170443079034	0.0
3	2.2803275006356413E-4	0.0	0.0	2.0716775343274803	0.0
4	2.2803275006356413E-4	0.0	0.0	4.870437492232634	0.0
5	2.2803275006356413E-4	0.0	0.0	5.183298425319845	0.0
6	2.2803275006356413E-4	0.0	0.0	6.779413659389761	0.0
7	2.2803275006356413E-4	0.0	0.0	7.984452727100666	0.0
8	2.2803275006356413E-4	0.0	0.0	8.603219594398148	0.0
9	2.2803275006356413E-4	0.0	0.0	10.98536371793717	0.0
10	2.2803275006356413E-4	0.0	0.0	12.551948710873855	0.0
11	2.2803275006356413E-4	0.0	0.0	15.72046377300708	0.0
12	2.2803275006356413E-4	0.0	0.0	16.605572892378802	0.0
13	2.2803275006356413E-4	0.0	0.0	16.988211846985465	0.0
14	2.2803275006356413E-4	0.0	0.0	17.476315948496524	0.0
15	2.2803275006356413E-4	0.0	0.0	17.796473929585765	0.0
16	2.2803275006356413E-4	0.0	0.0	18.35401400349118	0.0
17	2.2803275006356413E-4	0.0	0.0	19.014624880425327	0.0
18	2.2803275006356413E-4	0.0	0.0	19.859714252160895	0.0
19	2.2803275006356413E-4	0.0	0.0	20.24816804189418	0.0
20	2.2803275006356413E-4	0.0	0.0	20.597514214991556	0.0
21	2.2803275006356413E-4	0.0	0.0	21.015612262233102	0.0
22	2.2803275006356413E-4	0.0	0.0	21.428237523473122	0.0
23	2.2803275006356413E-4	0.0	0.0	21.868112698345737	0.0
24	2.2803275006356413E-4	0.0	0.0	22.18256986068339	0.0
25	2.2803275006356413E-4	0.0	0.0	22.45392883325903	0.0
26	2.2803275006356413E-4	0.0	0.0	22.682873714322852	0.0
27	2.2803275006356413E-4	0.0	0.0	22.925614576765515	0.0
28	2.2803275006356413E-4	0.0	0.0	23.153875359579143	0.0
29	2.2803275006356413E-4	0.0	0.0	23.417139169527527	0.0
30	2.2803275006356413E-4	0.0	0.0	23.740147559992565	0.0
31	2.2803275006356413E-4	0.0	0.0	24.00648981206681	0.0
32	2.2803275006356413E-4	0.0	0.0	24.23805706975636	0.0
33	2.2803275006356413E-4	0.0	0.0	24.473386867821958	0.0
34	2.2803275006356413E-4	0.0	0.0	24.708602649512525	0.0
35	2.2803275006356413E-4	0.0	0.0	24.9944417017172	0.0
36	2.2803275006356413E-4	0.0	0.0	25.248812234413105	0.0
37	2.2803275006356413E-4	0.0	0.0	25.493035309731184	0.0
38	3.4204912509534617E-4	0.0	0.0	25.742845187425818	0.0
39	3.4204912509534617E-4	0.0	0.0	25.997557769246818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGC	20	7.032371E-4	45.000004	41
GTATTAC	20	7.032371E-4	45.000004	43
AGATACG	35	1.2114833E-7	45.000004	1
CCGATAG	60	0.0	45.000004	1
ATTACGA	30	2.1649103E-6	45.000004	31
TTCACGT	35	1.2114833E-7	45.000004	29
CCTAATA	30	2.1649103E-6	45.000004	22
TCTAGCG	30	2.1649103E-6	45.000004	1
TCAATCG	20	7.032371E-4	45.000004	42
CTAACCG	20	7.032371E-4	45.000004	1
GTATGCG	35	1.2114833E-7	45.000004	1
TATTCGG	20	7.032371E-4	45.000004	39
TATTCGA	20	7.032371E-4	45.000004	38
GGTATAC	20	7.032371E-4	45.000004	30
TATTACG	20	7.032371E-4	45.000004	42
TGTTACG	35	1.2114833E-7	45.000004	1
CGAATAA	20	7.032371E-4	45.000004	22
TTAAACG	35	1.2114833E-7	45.000004	1
CGTCGGA	20	7.032371E-4	45.000004	24
GGCATAC	40	6.8121153E-9	45.000004	8
>>END_MODULE