FastQCFastQC Report
Sat 18 Jun 2016
SRR3552941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753076
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT74990.9957826301727847TruSeq Adapter, Index 13 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC38090.5057922440762951No Hit
AATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT37410.49676261094497765No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGT35250.46808024688079297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26810.35600656507444134No Hit
AAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT19950.26491350142615083No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT11740.15589396023774493No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10430.13849863758770695No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC9340.1240246668330952TruSeq Adapter, Index 19 (95% over 21bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8710.11565897731437465No Hit
AATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT8300.11021463969108031No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8290.11008185096856093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC302.164501E-645.00000413
TAGCCGT302.164501E-645.00000444
CCATGCG302.164501E-645.0000041
GGCCCAA302.164501E-645.00000424
AATGCGG600.045.0000042
ATATCCG302.164501E-645.0000041
TATTACG302.164501E-645.0000041
GTATCGA302.164501E-645.00000429
ACGTACC302.164501E-645.00000445
CGCACAA302.164501E-645.0000041
CGACTCG302.164501E-645.0000041
ATTGCGC302.164501E-645.00000429
TTACCGG1150.045.0000042
TATAATG302.164501E-645.0000041
CTTAGAC302.164501E-645.00000445
GCGTACA302.164501E-645.00000434
TTTAGCG207.031736E-445.01
ATCCTAC207.031736E-445.025
GTGCAAC207.031736E-445.014
AACGTAG351.2111741E-745.01
Produced by FastQC (version 0.11.5)