##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552941_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	753076
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.425760481013867	31.0	31.0	34.0	30.0	34.0
2	31.59724914882429	31.0	31.0	34.0	30.0	34.0
3	31.825327058623564	31.0	31.0	34.0	30.0	34.0
4	35.64180109311676	37.0	35.0	37.0	33.0	37.0
5	35.24951399327558	37.0	35.0	37.0	33.0	37.0
6	35.26767683474178	37.0	35.0	37.0	32.0	37.0
7	35.26700094014416	37.0	35.0	37.0	33.0	37.0
8	35.556793736621536	37.0	35.0	37.0	33.0	37.0
9	36.440304829791415	39.0	37.0	39.0	32.0	39.0
10	36.1331592030552	38.0	35.0	39.0	31.0	39.0
11	36.40053991894576	38.0	35.0	39.0	32.0	39.0
12	36.05279945184815	37.0	35.0	39.0	32.0	39.0
13	35.7599405637678	37.0	35.0	39.0	31.0	39.0
14	36.791973187301146	39.0	35.0	40.0	31.0	41.0
15	36.82738130016094	38.0	35.0	40.0	31.0	41.0
16	36.88054857677047	38.0	35.0	40.0	32.0	41.0
17	36.83867099734954	38.0	35.0	40.0	32.0	41.0
18	36.71484020205132	38.0	35.0	40.0	31.0	41.0
19	36.72167350971217	38.0	35.0	40.0	31.0	41.0
20	36.6879531415156	38.0	35.0	40.0	31.0	41.0
21	36.69191024544668	38.0	35.0	40.0	31.0	41.0
22	36.64421253631772	38.0	35.0	40.0	31.0	41.0
23	36.58735506110937	38.0	35.0	40.0	31.0	41.0
24	36.258718907520624	38.0	35.0	40.0	30.0	41.0
25	36.33165975280051	38.0	35.0	40.0	31.0	41.0
26	36.24952196059893	38.0	35.0	40.0	30.0	41.0
27	36.17861145488636	38.0	35.0	40.0	30.0	41.0
28	36.07440152122761	38.0	35.0	40.0	30.0	41.0
29	35.95446674704811	38.0	35.0	40.0	30.0	41.0
30	35.884006395104876	38.0	35.0	40.0	30.0	41.0
31	35.69865724043788	38.0	34.0	40.0	29.0	41.0
32	35.678424222787605	38.0	34.0	40.0	29.0	41.0
33	35.629951027519134	38.0	34.0	40.0	29.0	41.0
34	35.52183843330554	38.0	34.0	40.0	29.0	41.0
35	35.44743160052903	38.0	34.0	40.0	28.0	41.0
36	35.32523543440503	38.0	34.0	40.0	27.0	41.0
37	35.20171536471751	38.0	34.0	40.0	27.0	41.0
38	35.14074276699829	37.0	34.0	40.0	27.0	41.0
39	35.155717351236795	37.0	34.0	40.0	27.0	41.0
40	34.97885074016434	37.0	34.0	40.0	26.0	41.0
41	35.08882768804211	37.0	34.0	40.0	27.0	41.0
42	35.00149785679002	37.0	34.0	40.0	27.0	41.0
43	34.889375043156335	37.0	34.0	40.0	26.0	41.0
44	34.71738974552369	37.0	34.0	40.0	26.0	41.0
45	34.46485215303635	37.0	33.0	40.0	25.0	41.0
46	34.46426788265726	37.0	33.0	40.0	25.0	41.0
47	34.304824479866575	36.0	33.0	40.0	24.0	41.0
48	34.287063988229605	36.0	33.0	40.0	24.0	41.0
49	34.15360866632319	36.0	33.0	40.0	24.0	41.0
50	33.89121549485046	36.0	33.0	39.0	23.0	41.0
51	32.014624022011056	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	5.0
11	1.0
12	12.0
13	17.0
14	37.0
15	65.0
16	145.0
17	289.0
18	662.0
19	1145.0
20	1869.0
21	2849.0
22	3910.0
23	5081.0
24	6083.0
25	6941.0
26	7865.0
27	9348.0
28	11760.0
29	14835.0
30	18573.0
31	24670.0
32	32645.0
33	45800.0
34	74673.0
35	81720.0
36	76150.0
37	103129.0
38	129763.0
39	93027.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.78286388093632	34.142769122903935	20.25187364887475	25.822493347285004
2	21.0171350567539	34.09443402790688	21.099198487270872	23.78923242806835
3	20.6943256723093	31.845258645873724	21.9246131864513	25.535802495365672
4	20.163037993509285	32.86653671077023	20.288921702457653	26.68150359326283
5	17.600215648885374	36.8835283557038	18.696253764560282	26.820002230850537
6	20.940648752582742	36.60626550308335	20.716501388970038	21.736584355363867
7	79.54256940866526	8.220684233729397	6.800508846384694	5.436237511220647
8	81.91590224625403	6.0102831586719	5.320711322628792	6.753103272445278
9	74.5887533263575	10.907000090296332	9.715752460575027	4.788494122771141
10	42.163606329241674	31.026483382819265	12.213508331164451	14.596401956774615
11	33.19040840499498	23.353021474592207	25.09109306364829	18.365477056764522
12	31.991591818090075	23.71301170134223	27.025824750755568	17.26957172981213
13	23.10205078903059	33.252686315856565	23.36630034684414	20.2789625482687
14	18.266018303597512	32.31347168147703	29.580281405860763	19.840228609064688
15	16.021756104297573	28.044447041201686	33.36900923678353	22.5647876177172
16	18.66305658393044	25.29731394972088	33.545485449011785	22.494144017336897
17	18.117693300543372	27.097132294748473	27.681933828723793	27.103240575984362
18	21.942938030158977	26.282739059537153	27.18065640121316	24.593666509090717
19	22.6333065985372	30.8999357302583	26.111707184932197	20.3550504862723
20	27.081728802936222	28.530719343067634	24.674800418550053	19.71275143544609
21	21.21485746458525	30.50820899882615	24.480264940059172	23.796668596529432
22	20.48757363134664	27.02502801842045	26.457754595817683	26.029643754415225
23	22.640078823385686	28.20352793077989	22.86688196144878	26.289511284385643
24	18.881095666307253	30.360813516829644	27.614344368961437	23.143746447901673
25	18.732106719640516	28.417184985313565	26.91534453361945	25.935363761426473
26	21.809883730194564	31.113459996069455	24.647844307878618	22.428811965857363
27	19.653262087757412	29.354806155022867	26.24967466762983	24.742257089589895
28	19.7491355454164	30.286319043496274	29.486532567762087	20.478012843325242
29	20.986460861851924	26.723332040856434	29.419607051612324	22.87060004567932
30	23.272817086190503	28.231280773786445	25.289346626369714	23.20655551365334
31	25.4105827300299	28.2193297887597	24.58583197446207	21.784255506748323
32	28.314008147916013	25.69143087815838	23.799457159702342	22.195103814223266
33	23.870366337527688	26.554026419644234	24.65620999739734	24.91939724543074
34	20.27298705575533	27.38355756922276	28.209636212015788	24.133819163006123
35	22.384460532535893	25.98383164514604	28.375356537719966	23.2563512845981
36	25.633136628972373	25.11738523070713	29.48493910309185	19.764539037228644
37	23.91578008062931	27.527102178266205	27.320748503471098	21.236369237633387
38	23.179997769149463	25.738438085930238	28.644120912099176	22.43744323282112
39	23.7100903494468	24.3762913703265	28.872783092277537	23.040835187949156
40	23.701193505038002	24.78315601612586	25.467549091990715	26.048101386845417
41	19.419686724845832	20.980485369338552	32.79841609611779	26.80141180969783
42	24.94967307416516	22.725329183243126	27.339737290791366	24.98526045180035
43	22.32125310061667	22.468914160058215	28.934131482081487	26.27570125724362
44	23.256085707153062	22.9595684897673	27.15781674093983	26.62652906213981
45	23.623246524919132	25.9170389177188	26.603822190589	23.855892366773073
46	21.04063866063983	24.903329810005896	31.064726534904846	22.991304994449433
47	21.36929074887528	24.15187842926876	27.304813856768774	27.17401696508719
48	22.26813761160892	21.254826870063578	33.66300346844143	22.81403204988607
49	22.454041823136045	21.487207134472484	30.070537369402288	25.98821367298918
50	20.538830078239116	23.744748206024358	27.200441920868546	28.51597979486798
51	20.16476424690204	23.36855775512697	30.46478708656231	26.001890911408676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3186.0
1	2672.0
2	2158.0
3	1574.5
4	991.0
5	1002.0
6	1013.0
7	1066.0
8	1119.0
9	1216.0
10	1313.0
11	1411.0
12	1509.0
13	1652.0
14	1795.0
15	1900.5
16	2006.0
17	2059.0
18	2112.0
19	2116.5
20	2121.0
21	2211.0
22	2301.0
23	2824.5
24	3348.0
25	3221.5
26	4299.5
27	5504.0
28	5978.0
29	6452.0
30	8017.5
31	9583.0
32	11412.0
33	13241.0
34	13343.0
35	13445.0
36	16282.5
37	19120.0
38	20982.0
39	22844.0
40	29092.0
41	35340.0
42	49557.5
43	63775.0
44	70360.5
45	76946.0
46	79525.0
47	82104.0
48	74712.5
49	67321.0
50	61347.0
51	55373.0
52	50895.5
53	46418.0
54	42762.5
55	39107.0
56	35469.5
57	31832.0
58	29980.5
59	28129.0
60	28497.0
61	28865.0
62	25986.5
63	23108.0
64	20541.0
65	17974.0
66	15616.5
67	13259.0
68	11073.5
69	8888.0
70	7319.0
71	5750.0
72	4610.0
73	3470.0
74	2980.5
75	2177.0
76	1863.0
77	1507.5
78	1152.0
79	907.5
80	663.0
81	523.5
82	384.0
83	346.5
84	309.0
85	204.5
86	100.0
87	94.5
88	89.0
89	86.0
90	83.0
91	51.5
92	20.0
93	12.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	753076.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.99438168152659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56818642505252	20.93905230701677
2	10.051482481881942	5.426671091621986
3	3.7580911715844794	3.0434204243918064
4	1.885924689801224	2.036374835964355
5	0.9646328923232741	1.3019834238964703
6	0.6724307094730754	1.0891110735537548
7	0.42660315711065755	0.8061121914712541
8	0.3373801342546578	0.7285894492667933
9	0.2329747394214739	0.5660108134287719
>10	2.4213576111031623	15.65277534096923
>50	1.018630589990971	20.021044881532426
>100	0.651063313756043	23.70995848786149
>500	0.007331794073829313	1.3186566486047977
>1k	0.0034215039011203465	2.3707847370553337
>5k	4.887862715886209E-4	0.9894542933647567
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	7499	0.9957826301727847	TruSeq Adapter, Index 13 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	3809	0.5057922440762951	No Hit
AATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	3741	0.49676261094497765	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGT	3525	0.46808024688079297	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2681	0.35600656507444134	No Hit
AAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	1995	0.26491350142615083	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	1174	0.15589396023774493	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1043	0.13849863758770695	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	934	0.1240246668330952	TruSeq Adapter, Index 19 (95% over 21bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	871	0.11565897731437465	No Hit
AATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	830	0.11021463969108031	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	829	0.11008185096856093	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3278872251937387E-4	0.0	0.0	1.281145594866919	0.0
2	1.3278872251937387E-4	0.0	0.0	1.6352931178260892	0.0
3	1.3278872251937387E-4	0.0	0.0	2.088102661617154	0.0
4	1.3278872251937387E-4	0.0	0.0	4.535133240204176	0.0
5	1.3278872251937387E-4	0.0	0.0	4.79314172805932	0.0
6	1.3278872251937387E-4	0.0	0.0	6.356330569557389	0.0
7	1.3278872251937387E-4	0.0	0.0	7.555943888797413	0.0
8	1.3278872251937387E-4	0.0	0.0	8.294381974727651	0.0
9	1.3278872251937387E-4	0.0	0.0	9.921840557925096	0.0
10	1.3278872251937387E-4	0.0	0.0	11.642251246886104	0.0
11	1.3278872251937387E-4	0.0	0.0	14.008147916013789	0.0
12	1.3278872251937387E-4	0.0	0.0	14.901152074956578	0.0
13	1.3278872251937387E-4	0.0	0.0	15.270968667173035	0.0
14	1.3278872251937387E-4	0.0	0.0	15.659508469264722	0.0
15	1.3278872251937387E-4	0.0	0.0	15.99081633195056	0.0
16	1.3278872251937387E-4	0.0	0.0	16.600714934482045	0.0
17	1.3278872251937387E-4	0.0	0.0	17.31618057141643	0.0
18	1.3278872251937387E-4	0.0	0.0	18.2261816868417	0.0
19	1.3278872251937387E-4	0.0	0.0	18.65230069740637	0.0
20	1.3278872251937387E-4	0.0	0.0	19.0233123881255	0.0
21	1.3278872251937387E-4	0.0	0.0	19.475856354471528	0.0
22	1.3278872251937387E-4	0.0	0.0	19.955754797656546	0.0
23	1.3278872251937387E-4	0.0	0.0	20.407502031667455	0.0
24	1.3278872251937387E-4	0.0	0.0	20.742262401138795	0.0
25	1.3278872251937387E-4	0.0	0.0	21.02523516882758	0.0
26	1.3278872251937387E-4	0.0	0.0	21.282978079237687	0.0
27	1.3278872251937387E-4	0.0	0.0	21.53514386330198	0.0
28	1.3278872251937387E-4	0.0	0.0	21.798862266225456	0.0
29	1.3278872251937387E-4	0.0	0.0	22.07931204818637	0.0
30	1.3278872251937387E-4	0.0	0.0	22.47834215935709	0.0
31	1.3278872251937387E-4	0.0	0.0	22.779241404585992	0.0
32	1.3278872251937387E-4	0.0	0.0	23.068853608400747	0.0
33	1.3278872251937387E-4	0.0	0.0	23.359660910718176	0.0
34	1.3278872251937387E-4	0.0	0.0	23.61501362412293	0.0
35	1.3278872251937387E-4	0.0	0.0	23.92866058671369	0.0
36	1.3278872251937387E-4	0.0	0.0	24.22557617026701	0.0
37	1.3278872251937387E-4	0.0	0.0	24.510939134961145	0.0
38	1.3278872251937387E-4	0.0	0.0	24.80068412749842	0.0
39	1.3278872251937387E-4	0.0	0.0	25.08697661325019	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATC	30	2.164501E-6	45.000004	13
TAGCCGT	30	2.164501E-6	45.000004	44
CCATGCG	30	2.164501E-6	45.000004	1
GGCCCAA	30	2.164501E-6	45.000004	24
AATGCGG	60	0.0	45.000004	2
ATATCCG	30	2.164501E-6	45.000004	1
TATTACG	30	2.164501E-6	45.000004	1
GTATCGA	30	2.164501E-6	45.000004	29
ACGTACC	30	2.164501E-6	45.000004	45
CGCACAA	30	2.164501E-6	45.000004	1
CGACTCG	30	2.164501E-6	45.000004	1
ATTGCGC	30	2.164501E-6	45.000004	29
TTACCGG	115	0.0	45.000004	2
TATAATG	30	2.164501E-6	45.000004	1
CTTAGAC	30	2.164501E-6	45.000004	45
GCGTACA	30	2.164501E-6	45.000004	34
TTTAGCG	20	7.031736E-4	45.0	1
ATCCTAC	20	7.031736E-4	45.0	25
GTGCAAC	20	7.031736E-4	45.0	14
AACGTAG	35	1.2111741E-7	45.0	1
>>END_MODULE