##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552938_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476527
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.013289908022	31.0	31.0	34.0	28.0	34.0
2	31.225840298660938	31.0	31.0	34.0	28.0	34.0
3	31.24942343246028	31.0	31.0	34.0	28.0	34.0
4	35.273547983639965	37.0	35.0	37.0	33.0	37.0
5	34.866958220625484	35.0	35.0	37.0	32.0	37.0
6	34.918839855873856	35.0	35.0	37.0	32.0	37.0
7	34.678679277354696	35.0	35.0	37.0	32.0	37.0
8	35.14742501474208	37.0	35.0	37.0	32.0	37.0
9	35.61552650741721	39.0	35.0	39.0	31.0	39.0
10	35.582795938110536	37.0	35.0	39.0	30.0	39.0
11	35.92659597462473	37.0	35.0	39.0	31.0	39.0
12	35.28452322743517	37.0	35.0	39.0	30.0	39.0
13	34.71117061572587	37.0	33.0	39.0	27.0	39.0
14	35.58988892549635	38.0	33.0	40.0	27.0	41.0
15	35.88530765308157	38.0	34.0	40.0	30.0	41.0
16	36.02118452889343	37.0	34.0	40.0	31.0	41.0
17	36.03558874943078	37.0	34.0	40.0	31.0	41.0
18	35.84510426481605	37.0	34.0	40.0	30.0	41.0
19	35.77043273518604	37.0	34.0	40.0	30.0	41.0
20	35.667941165978	37.0	34.0	40.0	30.0	41.0
21	35.629454364600534	37.0	34.0	40.0	30.0	41.0
22	35.638971139096	37.0	34.0	40.0	30.0	41.0
23	35.544086693933394	37.0	34.0	40.0	30.0	41.0
24	35.13662184933907	36.0	34.0	40.0	27.0	41.0
25	35.305739234083276	36.0	34.0	40.0	29.0	41.0
26	35.09485716444189	36.0	34.0	40.0	28.0	41.0
27	35.006490713013115	36.0	34.0	40.0	27.0	41.0
28	34.878678437947904	36.0	33.0	40.0	27.0	41.0
29	34.74985677621625	36.0	33.0	40.0	27.0	41.0
30	34.676574464825705	36.0	33.0	40.0	26.0	41.0
31	34.449642937336186	36.0	33.0	39.0	25.0	41.0
32	34.31193825323644	36.0	33.0	39.0	25.0	41.0
33	34.18315226629341	35.0	33.0	39.0	25.0	41.0
34	34.19747044763466	36.0	33.0	39.0	24.0	41.0
35	33.99539585375015	35.0	33.0	40.0	23.0	41.0
36	33.860440226891654	35.0	33.0	39.0	23.0	41.0
37	33.577927378721455	35.0	33.0	39.0	22.0	41.0
38	33.47272872261173	35.0	32.0	39.0	22.0	41.0
39	33.49423222608583	35.0	33.0	39.0	22.0	40.0
40	33.21982804751882	35.0	32.0	39.0	21.0	40.0
41	33.37473637380463	35.0	32.0	39.0	22.0	40.0
42	33.29639453798001	35.0	32.0	39.0	21.0	40.0
43	33.22614878065671	35.0	32.0	39.0	21.0	40.0
44	33.1906586615239	35.0	32.0	39.0	21.0	40.0
45	32.950397354189796	35.0	32.0	39.0	20.0	40.0
46	32.779939017096616	35.0	31.0	39.0	20.0	40.0
47	32.6744528641609	35.0	31.0	39.0	20.0	40.0
48	32.63382557546582	35.0	31.0	39.0	20.0	40.0
49	32.52166404002291	35.0	31.0	39.0	18.0	40.0
50	32.37128011634178	35.0	31.0	39.0	18.0	40.0
51	30.52561554749259	34.0	28.0	37.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	7.0
10	7.0
11	10.0
12	8.0
13	23.0
14	27.0
15	75.0
16	186.0
17	297.0
18	714.0
19	1381.0
20	2305.0
21	3339.0
22	4499.0
23	5541.0
24	6271.0
25	6822.0
26	7442.0
27	8568.0
28	10545.0
29	12864.0
30	16062.0
31	20460.0
32	26568.0
33	36818.0
34	59847.0
35	58697.0
36	42682.0
37	51870.0
38	57316.0
39	35269.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.595645157567148	37.88536641155695	20.994613106917342	23.52437532395856
2	19.922900486226382	38.06499946487817	19.9317142575342	22.080385791361245
3	18.73304135967112	34.68302950305019	22.568710692153854	24.01521844512483
4	21.139410778402905	33.21050013955138	19.224304184233002	26.425784897812715
5	16.517217282546422	40.08587131474187	18.86776614966203	24.52914525304967
6	20.602819987115105	36.72719489137027	21.74944966392251	20.92053545759212
7	70.67637300719582	14.853303170649301	7.573547773788264	6.896776048366619
8	73.64640408623227	9.90437058131019	6.970643845994036	9.478581486463517
9	68.86703166871972	14.711023719537403	11.024349092496333	5.397595519246549
10	46.73984894874792	25.748803320693263	11.935525164366343	15.575822566192471
11	39.53668942158576	23.835165688408004	21.24706469937695	15.381080190629282
12	36.55511649917004	25.256491237642358	24.26326315193053	13.925129111257077
13	22.708471922892095	40.78782524390013	18.526127585635233	17.977575247572542
14	13.506265122437974	39.97842724546563	31.254472464309472	15.260835167786924
15	11.160752696069688	29.601890344093828	37.30113928486738	21.9362176749691
16	11.880124316145778	26.382135744669245	39.57173465511923	22.16600528406575
17	12.442526866263611	27.611027287016267	28.95470770806271	30.99173813865741
18	18.909316785827457	27.641455783198015	28.04458089468173	25.404646536292802
19	20.632828779901242	33.43818923166998	27.55394762521326	18.375034363215516
20	26.27951826444252	31.11974767431856	23.350618118175884	19.250115943063037
21	17.506038482604342	33.9229466535999	24.31006008054108	24.260954783254675
22	16.992531378075114	33.38719526910332	24.388334763822407	25.231938588999153
23	20.418150493046564	32.024628195254415	20.40430028099142	27.1529210307076
24	15.902561659675108	33.05730840015361	28.26093799511885	22.779191945052432
25	15.380240783838062	28.46386458689644	29.046203048305763	27.109691580959737
26	17.508766554675812	37.23755422043242	23.05367796578158	22.200001259110184
27	14.429402741082878	34.5084328904763	27.84627104025585	23.215893328184972
28	15.952716215450542	30.68703347344432	30.514955081244082	22.84529522986106
29	15.325259639013108	30.600994277344203	29.292988644924634	24.780757438718055
30	20.85233365580544	30.629743959943507	24.815802672251518	23.70211971199953
31	25.08651136242018	33.47911031274198	16.569680206997713	24.864698117840124
32	23.982901283662837	29.597693310137725	20.720127086188192	25.69927832001125
33	26.793445072367355	29.21072677938501	20.783712150623153	23.21211599762448
34	16.113462615969297	29.948145645472344	20.812671684920268	33.12572005363809
35	20.477538523525425	27.436850377837985	24.942553097725835	27.14305800091076
36	24.25948582137004	25.83820014395826	26.85178384435719	23.050530190314504
37	19.413170712257646	31.854858171730037	24.15183190039599	24.580139215616324
38	22.105777846795668	33.384677048729664	22.325912277793282	22.183632826681386
39	21.558694470617613	27.312618172737324	24.77194366741024	26.35674368923482
40	25.43738340115041	28.35809933120264	18.84971890365079	27.354798363996164
41	18.211979594020907	22.670278913891554	27.222801646076718	31.89493984601082
42	24.748440277256062	26.070086270032967	21.20761258018958	27.97386087252139
43	21.64830114558042	24.179322472808465	26.134510741259152	28.03786564035196
44	19.774535335878134	26.35548457904799	24.789990913421487	29.079989171652393
45	23.0299647239296	31.52748952315399	23.159023518079774	22.283522234836642
46	19.79992739131256	26.38906085069681	30.653247350097683	23.15776440789294
47	20.77657719289778	26.820935644779837	23.593836235932066	28.80865092639032
48	21.43257360023671	20.567564901883838	34.790683424024245	23.209178073855206
49	22.11585072829032	20.879194673124505	30.29880783250477	26.706146766080412
50	18.959051638207278	24.950737313940238	25.08294388355749	31.007267164294994
51	17.830049504015513	26.45243606343397	30.574762815118557	25.142751617431962
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4596.0
1	3788.0
2	2980.0
3	2138.0
4	1296.0
5	1227.5
6	1159.0
7	1227.0
8	1295.0
9	1375.0
10	1455.0
11	1539.5
12	1624.0
13	1833.0
14	2042.0
15	2141.0
16	2240.0
17	2393.5
18	2547.0
19	2535.0
20	2523.0
21	2380.0
22	2237.0
23	2234.0
24	2231.0
25	2513.5
26	2891.0
27	2986.0
28	4153.5
29	5321.0
30	5690.0
31	6059.0
32	5907.5
33	5756.0
34	6803.5
35	7851.0
36	8514.0
37	9177.0
38	10046.0
39	10915.0
40	15398.0
41	19881.0
42	25749.5
43	31618.0
44	37865.5
45	44113.0
46	51327.0
47	58541.0
48	54603.5
49	50666.0
50	50195.5
51	49725.0
52	43888.5
53	38052.0
54	33198.5
55	28345.0
56	23011.0
57	17677.0
58	15675.0
59	13673.0
60	12788.0
61	11903.0
62	10780.5
63	9658.0
64	8993.5
65	8329.0
66	6607.5
67	4886.0
68	4141.5
69	3397.0
70	2850.5
71	2304.0
72	1951.5
73	1599.0
74	1464.0
75	962.5
76	596.0
77	542.0
78	488.0
79	422.5
80	357.0
81	257.0
82	157.0
83	117.0
84	77.0
85	52.5
86	28.0
87	24.5
88	21.0
89	15.5
90	10.0
91	9.0
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	476527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.614265353819768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97526316605978	23.684086651412624
2	9.432733287329524	5.586869331655704
3	3.4743835200139612	3.0867394650789555
4	1.6538426084578162	1.9590933544129288
5	0.9785745335174866	1.4489882952038622
6	0.6367851883844511	1.1314755325319548
7	0.43406932070363746	0.8998250831688872
8	0.289750574732776	0.6864600325246573
9	0.22263788215855415	0.5933931588050292
>10	1.5476667707229232	10.50945529222654
>50	0.7028339044785692	15.206198712747888
>100	0.6386155781810349	27.289888346005554
>500	0.005708295670891933	1.2304507254430248
>1k	0.005708295670891933	4.088612585711332
>5k	0.0014270739177229832	2.598463433071076
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	6641	1.393625125124075	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	5656	1.186921202785991	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGT	4711	0.9886113483601139	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	4499	0.9441227884254197	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4016	0.842764418385527	No Hit
AAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	1757	0.36870943304366804	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	1193	0.2503530754815572	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1065	0.22349205816249657	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT	1061	0.22265265137127593	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT	1047	0.21971472760200367	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT	919	0.19285371028294304	No Hit
AACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	843	0.1769049812497508	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	796	0.16704195145290823	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	776	0.16284491749680502	No Hit
TCAAGAGGTTTCAGCATTCCCAATTATCAAAAAACAGAAAAACAAACAAAA	737	0.15466070128240372	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTAT	665	0.13955137904043213	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	544	0.11415932360600764	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT	543	0.11394947190820248	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.837881169377601	0.0
2	0.0	0.0	0.0	2.1937896488551543	0.0
3	0.0	0.0	0.0	2.7927063943910837	0.0
4	0.0	0.0	0.0	5.864305695165227	0.0
5	0.0	0.0	0.0	6.205104852400808	0.0
6	0.0	0.0	0.0	8.133851806928044	0.0
7	0.0	0.0	0.0	9.755375875868523	0.0
8	0.0	0.0	0.0	10.646825888144848	0.0
9	0.0	0.0	0.0	13.037456429541244	0.0
10	0.0	0.0	0.0	14.94060147693625	0.0
11	0.0	0.0	0.0	18.582787544042624	0.0
12	0.0	0.0	0.0	19.62470122364525	0.0
13	0.0	0.0	0.0	20.1512191334384	0.0
14	0.0	0.0	0.0	20.724743823539903	0.0
15	2.098516978051611E-4	0.0	0.0	21.09303355318796	0.0
16	2.098516978051611E-4	0.0	0.0	21.75574521485666	0.0
17	2.098516978051611E-4	0.0	0.0	22.521913763543303	0.0
18	2.098516978051611E-4	0.0	0.0	23.615660812503805	0.0
19	2.098516978051611E-4	0.0	0.0	24.03137702585583	0.0
20	2.098516978051611E-4	0.0	0.0	24.45842523088933	0.0
21	2.098516978051611E-4	0.0	0.0	24.901422164956024	0.0
22	2.098516978051611E-4	0.0	0.0	25.319446746983907	0.0
23	2.098516978051611E-4	0.0	0.0	25.745865396923993	0.0
24	2.098516978051611E-4	0.0	0.0	26.047212434972206	0.0
25	2.098516978051611E-4	0.0	0.0	26.2707044931347	0.0
26	2.098516978051611E-4	0.0	0.0	26.496924623368667	0.0
27	2.098516978051611E-4	0.0	0.0	26.71873786794872	0.0
28	2.098516978051611E-4	0.0	0.0	26.932366896314374	0.0
29	2.098516978051611E-4	0.0	0.0	27.140120077141486	0.0
30	2.098516978051611E-4	0.0	0.0	27.45174984838215	0.0
31	2.098516978051611E-4	0.0	0.0	27.71532358082543	0.0
32	2.098516978051611E-4	0.0	0.0	27.951406740856235	0.0
33	2.098516978051611E-4	0.0	0.0	28.160628883567984	0.0
34	2.098516978051611E-4	0.0	0.0	28.386429310406335	0.0
35	2.098516978051611E-4	0.0	0.0	28.65545918699255	0.0
36	2.098516978051611E-4	0.0	0.0	28.883358130808958	0.0
37	2.098516978051611E-4	0.0	0.0	29.10307285841096	0.0
38	2.098516978051611E-4	0.0	0.0	29.334119577694445	0.0
39	2.098516978051611E-4	0.0	0.0	29.566425407164758	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTGT	20	7.0291234E-4	45.000004	22
CTTCGTT	20	7.0291234E-4	45.000004	23
TCGCCGG	20	7.0291234E-4	45.000004	21
TTTAGCA	20	7.0291234E-4	45.000004	24
AGACTTC	35	1.209919E-7	45.000004	23
CAGGTCG	20	7.0291234E-4	45.000004	1
TCGTTTA	20	7.0291234E-4	45.000004	9
AGCAAGT	20	7.0291234E-4	45.000004	3
CGGAACG	40	6.8012014E-9	45.000004	1
GTCGCTC	20	7.0291234E-4	45.000004	17
TCGTTGT	20	7.0291234E-4	45.000004	25
TTGGCAA	55	1.8189894E-12	45.000004	30
ATCTCCC	20	7.0291234E-4	45.000004	11
GCCCAAC	20	7.0291234E-4	45.000004	40
ACCGGGA	160	0.0	45.000004	4
TCCGCTA	20	7.0291234E-4	45.000004	40
AATCATA	20	7.0291234E-4	45.000004	12
CATATGG	35	1.209919E-7	45.000004	2
TCCGCGC	20	7.0291234E-4	45.000004	39
GTATTGA	20	7.0291234E-4	45.000004	9
>>END_MODULE