FastQCFastQC Report
Sat 18 Jun 2016
SRR3552937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743923
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT52050.699669186192657Illumina PCR Primer Index 3 (95% over 24bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC46200.621032015410197No Hit
AATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT37580.5051598082059569Illumina PCR Primer Index 3 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGT37140.49924521758300255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27750.37302247678859235No Hit
AAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT14180.19061112507611674TruSeq Adapter, Index 14 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT8600.11560336217592412No Hit
AAAAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT8430.11331817943523725No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC7660.1029676458450673TruSeq Adapter, Index 14 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT302.1644646E-645.00000445
CGAACGC302.1644646E-645.00000413
CGAAACG351.2111559E-745.0000041
CTATCCG302.1644646E-645.0000041
CCGTCCA302.1644646E-645.00000423
ATATCCG302.1644646E-645.0000041
TCTACGC351.2111559E-745.00000439
TCGCTAG302.1644646E-645.00000418
TCTCGAG351.2111559E-745.0000041
GGTATCG302.1644646E-645.0000041
CGAATAC351.2111559E-745.00000440
CTCGCTA302.1644646E-645.00000410
CATAACG351.2111559E-745.0000041
TCGAGTG302.1644646E-645.00000435
ACGTAAC600.045.0000045
CATCCGC351.2111559E-745.00000419
CGTCACA600.045.00000428
TTCGAAA302.1644646E-645.00000431
CAATACG700.045.0000041
GCCCGTT302.1644646E-645.0000046
Produced by FastQC (version 0.11.5)