##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552937_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743923
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.484747749431058	31.0	31.0	34.0	30.0	34.0
2	31.6525715699071	31.0	31.0	34.0	30.0	34.0
3	31.857512134992465	31.0	31.0	34.0	30.0	34.0
4	35.67958108567688	37.0	35.0	37.0	33.0	37.0
5	35.281605757585126	37.0	35.0	37.0	33.0	37.0
6	35.29054619900178	37.0	35.0	37.0	32.0	37.0
7	35.32996291282834	37.0	35.0	37.0	33.0	37.0
8	35.63133953379584	37.0	35.0	37.0	35.0	37.0
9	36.52157548563494	39.0	37.0	39.0	33.0	39.0
10	36.100502336935406	38.0	35.0	39.0	31.0	39.0
11	36.39034685041328	38.0	35.0	39.0	32.0	39.0
12	36.09953987173404	37.0	35.0	39.0	32.0	39.0
13	35.86840035863927	37.0	35.0	39.0	31.0	39.0
14	36.89187187383641	39.0	35.0	40.0	31.0	41.0
15	36.903649974526935	38.0	35.0	40.0	32.0	41.0
16	36.94279918755032	38.0	35.0	40.0	32.0	41.0
17	36.90423605668866	38.0	35.0	40.0	32.0	41.0
18	36.76357768209882	38.0	35.0	40.0	31.0	41.0
19	36.78040469242112	38.0	35.0	40.0	31.0	41.0
20	36.7598689649332	38.0	35.0	40.0	31.0	41.0
21	36.77514877211755	38.0	35.0	40.0	31.0	41.0
22	36.77165513097458	38.0	35.0	40.0	31.0	41.0
23	36.730839078775624	38.0	35.0	40.0	31.0	41.0
24	36.35719960264705	38.0	35.0	40.0	30.0	41.0
25	36.428952996479474	38.0	35.0	40.0	31.0	41.0
26	36.3410984739012	38.0	35.0	40.0	31.0	41.0
27	36.26665394133533	38.0	35.0	40.0	30.0	41.0
28	36.20351434221015	38.0	35.0	40.0	30.0	41.0
29	36.0934868259215	38.0	35.0	40.0	30.0	41.0
30	36.021853068126674	38.0	35.0	40.0	30.0	41.0
31	35.8321130009423	38.0	34.0	40.0	30.0	41.0
32	35.82988696410784	38.0	34.0	40.0	30.0	41.0
33	35.7838176800556	38.0	34.0	40.0	30.0	41.0
34	35.644929649977215	38.0	34.0	40.0	29.0	41.0
35	35.60203811415966	38.0	34.0	40.0	29.0	41.0
36	35.4015429016175	38.0	34.0	40.0	28.0	41.0
37	35.30100427060327	38.0	34.0	40.0	27.0	41.0
38	35.172683194362854	38.0	34.0	40.0	27.0	41.0
39	35.02984448659337	38.0	34.0	40.0	26.0	41.0
40	34.821279890526306	37.0	34.0	40.0	25.0	41.0
41	34.87618476643416	37.0	34.0	40.0	26.0	41.0
42	34.780779731235626	37.0	34.0	40.0	25.0	41.0
43	34.65298827970099	37.0	33.0	40.0	25.0	41.0
44	34.46729701864306	37.0	33.0	40.0	24.0	41.0
45	34.039583397744124	36.0	33.0	40.0	23.0	41.0
46	33.92202284376206	36.0	33.0	40.0	23.0	41.0
47	33.75699904425593	36.0	33.0	40.0	23.0	41.0
48	33.73876328598524	36.0	33.0	40.0	23.0	41.0
49	33.62202808624011	36.0	33.0	39.0	22.0	41.0
50	33.330132285196186	36.0	32.0	39.0	21.0	41.0
51	31.247559223199175	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	10.0
10	15.0
11	8.0
12	7.0
13	11.0
14	26.0
15	56.0
16	116.0
17	265.0
18	540.0
19	998.0
20	1759.0
21	2529.0
22	3598.0
23	4963.0
24	5991.0
25	7013.0
26	8182.0
27	9757.0
28	12270.0
29	15359.0
30	19731.0
31	25152.0
32	33450.0
33	45480.0
34	73034.0
35	79873.0
36	75859.0
37	100671.0
38	126612.0
39	90587.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.23666831110209	35.858012186745135	19.27094605221239	25.634373449940384
2	19.997903008779133	34.240506073881306	21.40261828173077	24.35897263560879
3	20.53707171306708	31.63512890446995	22.57505145021729	25.25274793224568
4	20.45090688149177	31.867411008935065	19.708894603339324	27.972787506233843
5	17.25272642464341	37.106931765787586	18.515491522644144	27.124850286924858
6	21.216174254593554	35.635408503299395	21.3797664543239	21.768650787783145
7	80.64719063666536	7.994644607035943	6.33452655718401	5.023638199114693
8	82.92135073119127	5.639965426529359	4.645104399245621	6.793579443033755
9	75.3837426722927	10.867388157107657	8.766095415789	4.982773754810646
10	39.29976623924788	34.933722979394375	11.914942809941351	13.851567971416396
11	31.49371641957568	24.26339822804242	26.314282526551807	17.928602825830094
12	30.764474280268256	24.506165288611857	28.133287988138555	16.59607244298133
13	23.466810409141807	32.39165881415146	24.170377848245046	19.971152928461684
14	17.911665589046176	32.82691891499523	30.360265780194993	18.901149715763594
15	16.458020520941012	30.109164523747754	31.894026666738355	21.538788288572878
16	18.618862436031687	27.278764065635823	32.79815249696541	21.304221001367075
17	19.116494583444794	27.073500886516484	27.876272141068366	25.933732388970366
18	22.211438549419764	27.235748861105247	27.368692727607563	23.184119861867426
19	22.678556786118996	30.923496114517228	27.097965784093248	19.299981315270532
20	26.491720245240437	29.39134829814376	24.701750046711823	19.41518140990398
21	20.65227180770053	30.90749983533242	25.353027127807582	23.087201229159472
22	20.77889781603741	28.78900101220153	26.93813741475932	23.493963757001733
23	22.760151252212932	28.18974544408494	22.764587195180148	26.285516108521982
24	19.141228326049873	30.92793205748444	27.578123004665805	22.352716611799877
25	19.971152928461684	29.297790228289756	26.661361458107898	24.069695385140665
26	21.887345867784703	32.193385605768334	24.29982672937925	21.619441797067708
27	17.51148976439766	29.680087858555254	28.540588206037455	24.267834171009632
28	19.666013821322906	30.4938817592681	28.98283827761744	20.857266141791555
29	18.894831857598167	28.365973360146146	30.417395348712166	22.321799433543525
30	21.700498573105012	28.895463643414708	27.61024998554958	21.7937877979307
31	26.871060580194456	29.795287953188705	22.188183454470423	21.145468012146416
32	24.43599673622136	26.8100327587667	23.876799077323863	24.87717142768808
33	26.631788504993125	27.86538391742156	23.494367024544207	22.008460553041107
34	19.58441935522897	28.326184295955358	27.40942274939745	24.67997359941822
35	21.433266614958807	27.389797062330373	26.36254020913455	24.81439611357627
36	23.868330458931904	26.498979061004967	30.65182821340381	18.98086226665932
37	21.70184279824659	28.044837973822563	26.52908970417637	23.724229523754474
38	25.882786256104463	27.586053933001132	24.05343026092754	22.477729549966867
39	23.858920882940843	25.04761917564049	26.924560740829364	24.16889920058931
40	24.896125002184366	26.44937715328065	23.74748461870382	24.907013225831168
41	19.910259529548085	22.81808735581505	30.65357570608786	26.618077408549002
42	24.444196509584998	24.394056911804043	24.605906794117132	26.555839784493823
43	22.436596260634502	23.59881331804501	27.624229927022014	26.34036049429847
44	22.432429162695602	25.342273326674935	25.4635224344455	26.76177507618396
45	24.66908537577142	27.278091953065037	24.04307972733737	24.009742943826176
46	21.185122653823043	25.779818610259397	30.07166064229766	22.963398093619904
47	21.98560872563424	25.922575320295245	26.40125389321207	25.69056206085845
48	22.787573445101174	21.441600810836604	32.34004056871477	23.43078517534745
49	22.20485184622602	21.455984019851517	29.790583165193173	26.54858096872929
50	20.55226145716694	23.916588141514648	27.11100476796658	28.42014563335184
51	20.381679286700372	24.343782891508933	29.690572814659582	25.583965007131116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3288.0
1	2731.5
2	2175.0
3	1613.0
4	1051.0
5	1027.5
6	1004.0
7	1111.0
8	1218.0
9	1317.5
10	1417.0
11	1552.5
12	1688.0
13	1817.0
14	1946.0
15	2069.0
16	2192.0
17	2185.5
18	2179.0
19	2220.0
20	2261.0
21	2370.0
22	2479.0
23	2640.0
24	2801.0
25	2818.5
26	4063.5
27	5291.0
28	6065.5
29	6840.0
30	8374.0
31	9908.0
32	11406.5
33	12905.0
34	15393.5
35	17882.0
36	18252.5
37	18623.0
38	20577.0
39	22531.0
40	27151.5
41	31772.0
42	39506.5
43	47241.0
44	59894.5
45	72548.0
46	76661.5
47	80775.0
48	78830.5
49	76886.0
50	73594.0
51	70302.0
52	63666.0
53	57030.0
54	50041.0
55	43052.0
56	37630.0
57	32208.0
58	29069.0
59	25930.0
60	24995.0
61	24060.0
62	21765.0
63	19470.0
64	16097.5
65	12725.0
66	11114.5
67	9504.0
68	7649.5
69	5795.0
70	4854.5
71	3914.0
72	3486.5
73	3059.0
74	2331.0
75	1634.5
76	1666.0
77	1334.0
78	1002.0
79	697.5
80	393.0
81	278.0
82	163.0
83	159.0
84	155.0
85	112.5
86	70.0
87	53.0
88	36.0
89	33.0
90	30.0
91	18.5
92	7.0
93	8.5
94	10.0
95	5.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	743923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.494461033393577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01388406062524	23.789913475504207
2	9.956730841101276	6.0725028130790175
3	3.679572252737753	3.3661971804200284
4	1.721905317682303	2.1003429845302466
5	1.0049741030674466	1.5323071812779951
6	0.6089602312094117	1.1141948424901047
7	0.41986862624340643	0.8962567223486833
8	0.32066524939152985	0.7822811161866752
9	0.2649856895655826	0.7272536206378127
>10	2.6901617643698894	19.387216435396446
>50	0.902080826368434	19.700816434747367
>100	0.4096805297250387	16.880136299016325
>500	0.003918304747635865	0.7975066164472929
>1k	0.002176835970908813	2.162043490734837
>5k	4.3536719418176277E-4	0.6910307871829798
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	5205	0.699669186192657	Illumina PCR Primer Index 3 (95% over 24bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	4620	0.621032015410197	No Hit
AATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	3758	0.5051598082059569	Illumina PCR Primer Index 3 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGT	3714	0.49924521758300255	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2775	0.37302247678859235	No Hit
AAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1418	0.19061112507611674	TruSeq Adapter, Index 14 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	860	0.11560336217592412	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	843	0.11331817943523725	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	766	0.1029676458450673	TruSeq Adapter, Index 14 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.376900566322052E-4	0.0	0.0	1.079950478745784	0.0
2	5.376900566322052E-4	0.0	0.0	1.4100921735179581	0.0
3	5.376900566322052E-4	0.0	0.0	1.8973737873408942	0.0
4	5.376900566322052E-4	0.0	0.0	4.419812265516727	0.0
5	5.376900566322052E-4	0.0	0.0	4.703712615418532	0.0
6	5.376900566322052E-4	0.0	0.0	6.238952149617635	0.0
7	5.376900566322052E-4	0.0	0.0	7.373478169111588	0.0
8	5.376900566322052E-4	0.0	0.0	8.025427362778137	0.0
9	5.376900566322052E-4	0.0	0.0	9.784883650592871	0.0
10	5.376900566322052E-4	0.0	0.0	11.514363717750358	0.0
11	5.376900566322052E-4	0.0	0.0	14.057234418078215	0.0
12	5.376900566322052E-4	0.0	0.0	14.998998552269523	0.0
13	5.376900566322052E-4	0.0	0.0	15.37027353637406	0.0
14	5.376900566322052E-4	0.0	0.0	15.800156736651509	0.0
15	5.376900566322052E-4	0.0	0.0	16.1690121155012	0.0
16	5.376900566322052E-4	0.0	0.0	16.78507049788755	0.0
17	5.376900566322052E-4	0.0	0.0	17.5981922860296	0.0
18	6.721125707902565E-4	0.0	0.0	18.545064475758917	0.0
19	6.721125707902565E-4	0.0	0.0	19.020382485821784	0.0
20	6.721125707902565E-4	0.0	0.0	19.47526827373263	0.0
21	6.721125707902565E-4	0.0	0.0	20.010404302595834	0.0
22	6.721125707902565E-4	0.0	0.0	20.58586708570645	0.0
23	6.721125707902565E-4	0.0	0.0	21.147081082316316	0.0
24	6.721125707902565E-4	0.0	0.0	21.57992157790524	0.0
25	6.721125707902565E-4	0.0	0.0	21.939770648306343	0.0
26	6.721125707902565E-4	0.0	0.0	22.27582693370147	0.0
27	6.721125707902565E-4	0.0	0.0	22.627476230738935	0.0
28	6.721125707902565E-4	0.0	0.0	22.966893078988015	0.0
29	6.721125707902565E-4	0.0	0.0	23.3540299197632	0.0
30	6.721125707902565E-4	0.0	0.0	23.792650583460922	0.0
31	6.721125707902565E-4	0.0	0.0	24.155725794201818	0.0
32	6.721125707902565E-4	0.0	0.0	24.48465768634657	0.0
33	6.721125707902565E-4	0.0	0.0	24.832139885445134	0.0
34	6.721125707902565E-4	0.0	0.0	25.181907267284384	0.0
35	6.721125707902565E-4	0.0	0.0	25.596331878433656	0.0
36	6.721125707902565E-4	0.0	0.0	25.971908382991252	0.0
37	6.721125707902565E-4	0.0	0.0	26.32570844025524	0.0
38	6.721125707902565E-4	0.0	0.0	26.67964292003339	0.0
39	6.721125707902565E-4	0.0	0.0	27.07161897131827	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	30	2.1644646E-6	45.000004	45
CGAACGC	30	2.1644646E-6	45.000004	13
CGAAACG	35	1.2111559E-7	45.000004	1
CTATCCG	30	2.1644646E-6	45.000004	1
CCGTCCA	30	2.1644646E-6	45.000004	23
ATATCCG	30	2.1644646E-6	45.000004	1
TCTACGC	35	1.2111559E-7	45.000004	39
TCGCTAG	30	2.1644646E-6	45.000004	18
TCTCGAG	35	1.2111559E-7	45.000004	1
GGTATCG	30	2.1644646E-6	45.000004	1
CGAATAC	35	1.2111559E-7	45.000004	40
CTCGCTA	30	2.1644646E-6	45.000004	10
CATAACG	35	1.2111559E-7	45.000004	1
TCGAGTG	30	2.1644646E-6	45.000004	35
ACGTAAC	60	0.0	45.000004	5
CATCCGC	35	1.2111559E-7	45.000004	19
CGTCACA	60	0.0	45.000004	28
TTCGAAA	30	2.1644646E-6	45.000004	31
CAATACG	70	0.0	45.000004	1
GCCCGTT	30	2.1644646E-6	45.000004	6
>>END_MODULE