FastQCFastQC Report
Sat 18 Jun 2016
SRR3552935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744098
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT53100.7136156796550992Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24780.33302065050571295No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCC22470.3019763525772143No Hit
AATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT20230.2718727909495792No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGT18840.25319245583243066No Hit
AAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT13230.17779916086321962No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA12160.16341933455001895No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8360.11235079250313802No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT8140.10939419270042387No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA7450.10012122059191127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT406.8102963E-945.030
GTACCGG502.1827873E-1145.02
AGTCCAT207.031681E-445.037
CGTATCC207.031681E-445.038
GTTTCGG253.8894745E-545.023
ACGTTTG253.8894745E-545.033
CTATCGT207.031681E-445.043
TAGACGT253.8894745E-545.01
TTTCGGT253.8894745E-545.024
CGTGATA207.031681E-445.015
ATAATGC207.031681E-445.030
CGTGACG207.031681E-445.018
TAGCGGA207.031681E-445.03
ATTACGT207.031681E-445.029
TTGTCGG253.8894745E-545.040
CCAATGC406.8102963E-945.039
CGCGCGA253.8894745E-545.031
CGTCTTA351.2111559E-745.039
GATTTCG207.031681E-445.010
TCTAGTC253.8894745E-545.014