##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744098
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.597749221204733	31.0	31.0	34.0	30.0	34.0
2	31.78024937575427	31.0	31.0	34.0	30.0	34.0
3	31.982095100376565	33.0	31.0	34.0	30.0	34.0
4	35.792371434945395	37.0	35.0	37.0	35.0	37.0
5	35.41137323309564	37.0	35.0	37.0	33.0	37.0
6	35.42950122161328	37.0	35.0	37.0	33.0	37.0
7	35.41602718996691	37.0	35.0	37.0	33.0	37.0
8	35.729918639749066	37.0	35.0	37.0	35.0	37.0
9	36.61604519834753	39.0	37.0	39.0	33.0	39.0
10	36.32775790285688	38.0	35.0	39.0	32.0	39.0
11	36.54496988299928	38.0	35.0	39.0	32.0	39.0
12	36.16935403669947	37.0	35.0	39.0	32.0	39.0
13	35.90248999459749	37.0	35.0	39.0	31.0	39.0
14	36.970842550309236	39.0	35.0	40.0	32.0	41.0
15	37.012342460267334	39.0	35.0	40.0	32.0	41.0
16	37.08343255861459	39.0	35.0	40.0	32.0	41.0
17	37.07251195407056	38.0	35.0	40.0	32.0	41.0
18	36.91327352042339	38.0	35.0	40.0	32.0	41.0
19	36.95092716281995	39.0	35.0	40.0	32.0	41.0
20	36.89820561270155	39.0	35.0	40.0	32.0	41.0
21	36.894340530414006	39.0	35.0	40.0	32.0	41.0
22	36.87997145537281	39.0	35.0	40.0	32.0	41.0
23	36.83175334431755	38.0	35.0	40.0	32.0	41.0
24	36.49964923975068	38.0	35.0	40.0	31.0	41.0
25	36.58990079263753	38.0	35.0	40.0	31.0	41.0
26	36.48166504949617	38.0	35.0	40.0	31.0	41.0
27	36.38645850412177	38.0	35.0	40.0	31.0	41.0
28	36.39601638493854	38.0	35.0	40.0	31.0	41.0
29	36.22718109711355	38.0	35.0	40.0	30.0	41.0
30	36.036976312259945	38.0	35.0	40.0	30.0	41.0
31	35.94423852772081	38.0	35.0	40.0	30.0	41.0
32	35.884503116524975	38.0	34.0	40.0	30.0	41.0
33	35.68484661966569	38.0	34.0	40.0	29.0	41.0
34	35.68634239038406	38.0	34.0	40.0	29.0	41.0
35	35.48461089802687	38.0	34.0	40.0	28.0	41.0
36	35.306282237017165	38.0	34.0	40.0	27.0	41.0
37	35.174513303355205	38.0	34.0	40.0	27.0	41.0
38	35.088476249096225	37.0	34.0	40.0	27.0	41.0
39	35.035812487064874	37.0	34.0	40.0	27.0	41.0
40	34.751836451650185	37.0	33.0	40.0	25.0	41.0
41	34.87891245507984	37.0	34.0	40.0	26.0	41.0
42	34.82551491873382	37.0	34.0	40.0	26.0	41.0
43	34.748960486387546	37.0	33.0	40.0	26.0	41.0
44	34.633447476004505	37.0	33.0	40.0	26.0	41.0
45	34.386660090471956	36.0	33.0	40.0	24.0	41.0
46	34.30825509543098	36.0	33.0	40.0	24.0	41.0
47	34.18400802044892	36.0	33.0	40.0	24.0	41.0
48	34.189715601977156	36.0	33.0	40.0	24.0	41.0
49	34.018797255200255	36.0	33.0	39.0	24.0	41.0
50	33.79204217724009	35.0	33.0	39.0	23.0	41.0
51	31.844613209550356	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	9.0
11	3.0
12	8.0
13	14.0
14	40.0
15	62.0
16	114.0
17	243.0
18	468.0
19	923.0
20	1617.0
21	2515.0
22	3402.0
23	4577.0
24	5614.0
25	6550.0
26	7551.0
27	9059.0
28	11339.0
29	14368.0
30	18540.0
31	23944.0
32	31747.0
33	44625.0
34	75849.0
35	79797.0
36	72990.0
37	99261.0
38	129094.0
39	99763.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.339121997371315	33.39681063515827	21.61812019384543	24.64594717362498
2	22.56476969431446	32.58374568941188	20.988230044967196	23.863254571306467
3	21.206749648567797	32.80011503861051	20.49246201441208	25.50067329840962
4	20.44582837206927	32.09214377676059	19.3936820150034	28.068345836166742
5	18.536536854016543	35.50809167609643	18.02504508814699	27.930326381740038
6	21.861502113968857	36.72096954970985	19.84174127601472	21.575787060306574
7	82.14374988240796	7.644557571717704	5.394181949151859	4.8175105967224745
8	84.8075119137533	5.677881139312295	4.37039207201202	5.1442148749223895
9	76.70898188141885	10.785138516700757	8.027571637069311	4.478307964811087
10	44.58942236103309	30.587100086278955	11.170571618254584	13.652905934433369
11	35.248448457058075	23.999392553131443	23.090238113796836	17.661920876013642
12	33.75832215649014	24.053014522280666	25.378108797497106	16.810554523732087
13	24.982596378434025	32.07601686874578	22.77804805281025	20.163338700009945
14	18.52000677330137	33.689514015626976	27.769057301591992	20.021421909479663
15	16.50562157135216	29.22370440452736	33.234197646008994	21.036476378111484
16	19.380242924991062	25.647696943144588	32.64879088507159	22.323269246792762
17	19.231606589454614	26.27731831022258	27.435633478385913	27.055441621936893
18	22.582912465831114	26.51142725823749	26.574725372195594	24.3309349037358
19	24.557652352243924	30.027496378165242	24.957196498310708	20.457654771280126
20	27.48253590252897	28.36212434383643	23.958672110394062	20.19666764324054
21	23.019145327631577	28.595561337350727	25.109998951750978	23.27529438326672
22	21.64459520116974	28.12855295942201	25.078954653822482	25.147897185585773
23	23.289405427779673	27.884767866598214	23.31668678050472	25.509139925117392
24	21.286577843241076	28.552825031111496	27.5045760101492	22.656021115498227
25	20.397044475324485	27.385774454440142	27.14655865222054	25.070622418014832
26	22.883948082107462	30.445048904848555	23.31238627170077	23.358616741343212
27	20.447709844670996	30.19682891232069	25.765826544353025	23.589634698655285
28	21.035535641810622	27.25998457192467	30.396936962604386	21.307542823660324
29	21.49797472913514	27.5379049533798	26.98340809947077	23.980712218014293
30	24.039978604968702	27.958548470765948	26.09884719485874	21.902625729406612
31	28.06041677305946	27.415340452467284	22.19721058247704	22.327032191996217
32	28.978844184502577	26.192517652244728	22.2786514679518	22.549986695300888
33	26.30339014484651	26.84552303594419	25.3286529462517	21.522433872957595
34	23.868764598211527	26.369510467707208	25.27865953140581	24.483065402675454
35	24.23484541014759	25.906130644081827	26.816763383317788	23.042260562452796
36	28.149114767140887	25.45793699217039	26.514518248940327	19.878429991748398
37	26.124919029482673	29.50431260398496	23.322868761910392	21.04789960462197
38	25.68371370437765	27.594214740531488	25.651191106547795	21.07088044854307
39	25.44181008415558	25.775233907361667	26.06094896102395	22.722007047458803
40	25.551607449556375	25.91661313429145	24.770661928939468	23.761117487212704
41	21.997236923093464	23.73974933409309	28.673104886721912	25.589908856091537
42	25.638423971036072	25.600928909901654	23.751978906004318	25.008668213057955
43	23.709645772465453	25.07290706331693	25.88731591806456	25.33013124615306
44	23.62847366879094	25.614502390814113	24.839873242503003	25.917150697891945
45	24.741364712712574	27.966477533873224	24.687742743563348	22.604415009850854
46	21.962698461761757	26.679415883391705	28.646495488497482	22.71139016634906
47	22.6811522138213	25.987840311356834	26.883018097078608	24.447989377743255
48	24.050192313378076	22.329854400898807	30.49544549239482	23.1245077933283
49	22.805732578235663	23.758698451010485	28.487645444551656	24.947923526202192
50	21.237793946496293	25.430252466744975	25.90854968028405	27.423403906474686
51	22.238871761515284	25.228934898360162	28.250983069434405	24.281210270690153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2983.0
1	2501.0
2	2019.0
3	1487.0
4	955.0
5	903.0
6	851.0
7	881.0
8	911.0
9	999.0
10	1087.0
11	1192.0
12	1297.0
13	1399.0
14	1501.0
15	1680.0
16	1859.0
17	1973.0
18	2087.0
19	2123.0
20	2159.0
21	2275.0
22	2391.0
23	2636.5
24	2882.0
25	2927.5
26	3757.0
27	4541.0
28	5713.5
29	6886.0
30	8891.5
31	10897.0
32	12568.0
33	14239.0
34	16603.5
35	18968.0
36	19119.0
37	19270.0
38	21700.5
39	24131.0
40	26115.5
41	28100.0
42	35330.0
43	42560.0
44	46867.5
45	51175.0
46	56710.0
47	62245.0
48	62420.5
49	62596.0
50	66151.0
51	69706.0
52	64808.0
53	59910.0
54	55357.0
55	50804.0
56	45272.0
57	39740.0
58	36632.0
59	33524.0
60	32484.0
61	31444.0
62	28574.0
63	25704.0
64	21849.5
65	17995.0
66	16267.5
67	14540.0
68	12395.0
69	10250.0
70	8826.5
71	7403.0
72	6119.5
73	4836.0
74	3688.5
75	2209.0
76	1877.0
77	1486.5
78	1096.0
79	775.0
80	454.0
81	435.0
82	416.0
83	280.5
84	145.0
85	100.5
86	56.0
87	48.0
88	40.0
89	28.0
90	16.0
91	14.5
92	13.0
93	11.0
94	9.0
95	12.5
96	16.0
97	8.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	744098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.601308645734296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9606386061229	19.95894371173901
2	9.990638583243271	5.115468438751852
3	3.6131660229435063	2.77505335624971
4	1.6944231141459665	1.7351779648686863
5	0.9226834814755558	1.1810952295788184
6	0.5681351034403694	0.8727001281391847
7	0.37008226897944463	0.6632213274719495
8	0.28289910980215593	0.5794069940518777
9	0.20455913431906858	0.47132833806060237
>10	2.360150201059591	15.041740075662172
>50	1.3154423075208719	24.944781834640263
>100	0.7104014364961975	23.8834608598228
>500	0.003129521746679284	0.5768629354819717
>1k	0.003129521746679284	1.491698113951182
>5k	5.215869577798807E-4	0.7090606915299235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	5310	0.7136156796550992	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2478	0.33302065050571295	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCC	2247	0.3019763525772143	No Hit
AATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	2023	0.2718727909495792	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGT	1884	0.25319245583243066	No Hit
AAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	1323	0.17779916086321962	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1216	0.16341933455001895	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	836	0.11235079250313802	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT	814	0.10939419270042387	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	745	0.10012122059191127	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.687818002467417E-4	0.0	0.0	1.0106195689277488	0.0
2	2.687818002467417E-4	0.0	0.0	1.2636776338600562	0.0
3	2.687818002467417E-4	0.0	0.0	1.5964295025655222	0.0
4	2.687818002467417E-4	0.0	0.0	3.338404349964655	0.0
5	2.687818002467417E-4	0.0	0.0	3.5437536453531657	0.0
6	2.687818002467417E-4	0.0	0.0	4.698709041013415	0.0
7	2.687818002467417E-4	0.0	0.0	5.601547108042221	0.0
8	2.687818002467417E-4	0.0	0.0	6.037645578942558	0.0
9	2.687818002467417E-4	0.0	0.0	7.202142728511567	0.0
10	2.687818002467417E-4	0.0	0.0	8.432491419141027	0.0
11	2.687818002467417E-4	0.0	0.0	10.207257646170262	0.0
12	2.687818002467417E-4	0.0	0.0	10.877196283285267	0.0
13	2.687818002467417E-4	0.0	0.0	11.12568505761338	0.0
14	2.687818002467417E-4	0.0	0.0	11.447954436109223	0.0
15	2.687818002467417E-4	0.0	0.0	11.711629382151276	0.0
16	2.687818002467417E-4	0.0	0.0	12.19704931339689	0.0
17	2.687818002467417E-4	0.0	0.0	12.793341737244289	0.0
18	2.687818002467417E-4	0.0	0.0	13.52227797951345	0.0
19	2.687818002467417E-4	0.0	0.0	13.854761066418671	0.0
20	2.687818002467417E-4	0.0	0.0	14.186706589723396	0.0
21	2.687818002467417E-4	0.0	0.0	14.588669771992398	0.0
22	2.687818002467417E-4	0.0	0.0	15.002593744372382	0.0
23	2.687818002467417E-4	0.0	0.0	15.419877489255448	0.0
24	2.687818002467417E-4	0.0	0.0	15.712312087923902	0.0
25	2.687818002467417E-4	0.0	0.0	15.979481197369164	0.0
26	2.687818002467417E-4	0.0	0.0	16.2240726355937	0.0
27	2.687818002467417E-4	0.0	0.0	16.469470419218975	0.0
28	2.687818002467417E-4	0.0	0.0	16.716615284545853	0.0
29	2.687818002467417E-4	0.0	0.0	16.98244048498988	0.0
30	2.687818002467417E-4	0.0	0.0	17.299468618380914	0.0
31	2.687818002467417E-4	0.0	0.0	17.56569699152531	0.0
32	2.687818002467417E-4	0.0	0.0	17.821442874460086	0.0
33	2.687818002467417E-4	0.0	0.0	18.093315665409662	0.0
34	4.031727003701125E-4	0.0	0.0	18.359006474953567	0.0
35	5.375636004934834E-4	0.0	0.0	18.65412889162449	0.0
36	5.375636004934834E-4	0.0	0.0	18.914578456063584	0.0
37	5.375636004934834E-4	0.0	0.0	19.186988810613656	0.0
38	5.375636004934834E-4	0.0	0.0	19.452410838357313	0.0
39	5.375636004934834E-4	0.0	0.0	19.73933541012071	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	40	6.8102963E-9	45.0	30
GTACCGG	50	2.1827873E-11	45.0	2
AGTCCAT	20	7.031681E-4	45.0	37
CGTATCC	20	7.031681E-4	45.0	38
GTTTCGG	25	3.8894745E-5	45.0	23
ACGTTTG	25	3.8894745E-5	45.0	33
CTATCGT	20	7.031681E-4	45.0	43
TAGACGT	25	3.8894745E-5	45.0	1
TTTCGGT	25	3.8894745E-5	45.0	24
CGTGATA	20	7.031681E-4	45.0	15
ATAATGC	20	7.031681E-4	45.0	30
CGTGACG	20	7.031681E-4	45.0	18
TAGCGGA	20	7.031681E-4	45.0	3
ATTACGT	20	7.031681E-4	45.0	29
TTGTCGG	25	3.8894745E-5	45.0	40
CCAATGC	40	6.8102963E-9	45.0	39
CGCGCGA	25	3.8894745E-5	45.0	31
CGTCTTA	35	1.2111559E-7	45.0	39
GATTTCG	20	7.031681E-4	45.0	10
TCTAGTC	25	3.8894745E-5	45.0	14
>>END_MODULE