FastQCFastQC Report
Sat 18 Jun 2016
SRR3552933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662361
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT71251.0756973916036723TruSeq Adapter, Index 27 (100% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC43560.6576474158351714No Hit
AATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT38260.5776306274071088TruSeq Adapter, Index 27 (100% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGT36080.5447180616008491No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18930.2857958122534388No Hit
AAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT15220.22978406035379498TruSeq Adapter, Index 27 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9660.14584192004058208No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT9060.1367834156902354No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA8740.13195221337005048No Hit
AATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT8710.13149928815253314No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC8260.12470540988977309TruSeq Adapter, Index 27 (96% over 25bp)
AATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT6880.10387084988397566No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCA253.8890084E-545.00000434
ACGTTAG502.1827873E-1145.0000041
ATAGCCG253.8890084E-545.0000041
ATAGACG253.8890084E-545.0000041
GGTCAAT253.8890084E-545.0000048
TCTAGCG253.8890084E-545.0000041
GCGCAAA253.8890084E-545.00000425
CCCTTGT253.8890084E-545.00000415
CATACCG453.8380676E-1045.0000041
CTCGACT253.8890084E-545.00000422
TATAGCG253.8890084E-545.0000041
TTAAACG253.8890084E-545.0000041
TTCGGGC253.8890084E-545.0000044
GTGCGGC253.8890084E-545.00000421
ATCGACG253.8890084E-545.0000041
GGTAGAC253.8890084E-545.0000049
AGGTACG207.031119E-445.011
TACCCTT207.031119E-445.032
GTCGCAT207.031119E-445.042
ACAGTCG207.031119E-445.01
Produced by FastQC (version 0.11.5)