##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	662361
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315504687021125	31.0	31.0	34.0	30.0	34.0
2	31.489539692101438	31.0	31.0	34.0	30.0	34.0
3	31.71445178686547	31.0	31.0	34.0	30.0	34.0
4	35.541340749228894	37.0	35.0	37.0	33.0	37.0
5	35.123029586584956	37.0	35.0	37.0	32.0	37.0
6	35.143076056712275	37.0	35.0	37.0	32.0	37.0
7	35.150677651612945	37.0	35.0	37.0	32.0	37.0
8	35.45072098145875	37.0	35.0	37.0	33.0	37.0
9	36.3108093622662	39.0	37.0	39.0	32.0	39.0
10	36.010781129927636	38.0	35.0	39.0	31.0	39.0
11	36.267708998567244	38.0	35.0	39.0	32.0	39.0
12	35.87059473610312	37.0	35.0	39.0	31.0	39.0
13	35.56272183899716	37.0	35.0	39.0	30.0	39.0
14	36.576754971986574	38.0	35.0	40.0	31.0	41.0
15	36.59937254759867	38.0	35.0	40.0	31.0	41.0
16	36.64723919433662	38.0	35.0	40.0	31.0	41.0
17	36.62552143015667	38.0	35.0	40.0	31.0	41.0
18	36.46607665608332	38.0	35.0	40.0	31.0	41.0
19	36.469582297266896	38.0	35.0	40.0	31.0	41.0
20	36.45625421786609	38.0	35.0	40.0	31.0	41.0
21	36.45800099945498	38.0	35.0	40.0	31.0	41.0
22	36.433298458091585	38.0	35.0	40.0	31.0	41.0
23	36.38498190563756	38.0	35.0	40.0	31.0	41.0
24	36.017170394996086	38.0	34.0	40.0	30.0	41.0
25	36.170013029148755	38.0	35.0	40.0	30.0	41.0
26	35.977768920573524	38.0	34.0	40.0	30.0	41.0
27	35.94536967001378	38.0	34.0	40.0	30.0	41.0
28	35.932683234671124	38.0	34.0	40.0	30.0	41.0
29	35.63454973949251	38.0	34.0	40.0	29.0	41.0
30	35.678334020269915	38.0	34.0	40.0	29.0	41.0
31	35.45267308914625	38.0	34.0	40.0	28.0	41.0
32	35.423699462981666	38.0	34.0	40.0	28.0	41.0
33	35.447832828321715	38.0	34.0	40.0	29.0	41.0
34	35.27066660023763	38.0	34.0	40.0	27.0	41.0
35	35.18434358303101	37.0	34.0	40.0	27.0	41.0
36	34.914815334840064	37.0	34.0	40.0	26.0	41.0
37	34.77554083045349	37.0	33.0	40.0	25.0	41.0
38	34.69057960840086	37.0	33.0	40.0	25.0	41.0
39	34.60191647757039	37.0	33.0	40.0	25.0	41.0
40	34.41593934425487	37.0	33.0	40.0	24.0	41.0
41	34.54511361629081	37.0	33.0	40.0	25.0	41.0
42	34.36468934614206	37.0	33.0	40.0	24.0	41.0
43	34.199536204577264	36.0	33.0	40.0	23.0	41.0
44	34.011526946785814	36.0	33.0	40.0	23.0	41.0
45	33.61608850762651	36.0	33.0	40.0	22.0	41.0
46	33.587898442088225	35.0	33.0	39.0	22.0	41.0
47	33.446786571069254	35.0	32.0	39.0	22.0	41.0
48	33.439124586139584	35.0	32.0	39.0	22.0	41.0
49	33.318617793016195	35.0	32.0	39.0	21.0	41.0
50	33.065746624574814	35.0	32.0	39.0	20.0	40.0
51	31.118500334409784	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	5.0
12	10.0
13	17.0
14	37.0
15	57.0
16	131.0
17	268.0
18	586.0
19	1058.0
20	1786.0
21	2616.0
22	3779.0
23	5071.0
24	6054.0
25	7196.0
26	8661.0
27	10350.0
28	12810.0
29	15878.0
30	19874.0
31	25070.0
32	31941.0
33	43082.0
34	67882.0
35	71067.0
36	65034.0
37	85288.0
38	103049.0
39	73692.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.649799731566322	34.45809762350138	20.70592924402252	26.186173400909773
2	20.661693547778327	34.13999314573171	21.93411145885703	23.264201847632936
3	20.509510674692503	31.044098308928213	22.7145016086394	25.73188940773989
4	21.104503435437774	31.095580808652684	20.16090923227666	27.63900652363288
5	17.651250602013103	36.463348536523135	19.27030727956507	26.61509358189869
6	21.087594227317126	36.06673702105046	21.39075217290873	21.454916578723687
7	77.4502121954644	8.819510810570067	7.446694476275022	6.283582517690505
8	80.01376892661253	5.875496896707385	6.156310531568133	7.954423645111955
9	73.17897641920342	10.826875374606898	10.597997164688138	5.396151041501537
10	42.582368225182336	27.04220206201754	13.482979825201062	16.89244988759906
11	34.848670136073835	23.48719202972397	23.622918619906667	18.041219214295527
12	33.53262042904096	22.38748960159188	26.92821588227568	17.151674087091482
13	24.841136479955793	30.846622914090656	22.542993926272832	21.769246679680716
14	18.371552672938172	31.83249013755339	29.634142106796745	20.161815082711694
15	16.73286923596045	28.154284446095108	32.4205380449634	22.692308272981048
16	18.48538787760753	24.752665087467406	33.21315717561873	23.54878985930633
17	18.280363729144682	26.406747981840716	27.66059595900121	27.65229233001339
18	21.466692634379136	26.517563685059958	26.335638722690497	25.6801049578704
19	22.696535575011207	30.717237276953202	26.90964594835747	19.676581199678118
20	27.22775042612714	27.804173252954207	24.23874594065774	20.729330380260915
21	21.494773997865213	31.077010874734473	24.045346872777838	23.38286825462248
22	20.9770804742429	28.36187517079055	25.58408481175673	25.07695954320982
23	23.566302967717	28.07426765766704	21.554107201359983	26.805322173255973
24	19.331905109147428	29.74918511204615	27.376460872545334	23.542448906261086
25	19.959659460626455	25.799526240222477	25.754837618760767	28.4859766803903
26	21.876740931304834	33.07788351065356	21.82963670868303	23.215738849358583
27	17.859747177143582	29.105578377954018	25.841799260524095	27.192875184378305
28	19.279214808842912	26.397387527345362	26.967167450982167	27.35623021282956
29	19.792681030435062	29.114183957086848	27.283158277736764	23.809976734741326
30	21.535537267441775	26.53658654419569	24.2429732426879	27.68490294567464
31	23.869762863453616	28.451403388786478	21.165195414585096	26.51363833317481
32	24.23874594065774	25.220083911945302	23.82975446923958	26.71141567815738
33	23.456393114932794	24.660570293238884	25.791071636162155	26.09196495566617
34	18.17981433085583	25.899320763148797	26.96626160054713	28.95460330544824
35	19.858506162047583	24.487854810292273	26.151902059450965	29.50173696820918
36	23.009959825533205	25.769935126011344	28.203049394514473	23.017055653940975
37	18.959449605275672	27.815647358464645	24.950140482304967	28.27476255395472
38	21.880968233334993	24.73651075470929	28.11518190231611	25.267339109639607
39	20.82474662608457	23.854665356203036	30.89946418946768	24.421123828244717
40	22.055495417151675	24.110115178882815	26.19794945656523	27.636439947400284
41	18.290328083930063	21.027959073677348	31.07429332342937	29.607419518963223
42	23.022188806406174	22.535747122792557	24.451922743035897	29.990141327765375
43	20.940695481769005	23.68753595093914	27.255076914250687	28.116691653041165
44	21.499756175257904	22.461014461902195	25.831834905738717	30.207394457101188
45	23.00558154843054	25.854028241397064	24.88884460286762	26.251545607304777
46	18.410806191789675	24.127175362075967	32.327084475082316	25.134933971052035
47	20.936619154811346	24.074787011916463	26.654347100750197	28.334246732521994
48	21.205656734016646	20.527476708320687	33.41621864813901	24.85064790952366
49	20.961530041774804	20.665316949518463	29.948019282536258	28.425133726170472
50	18.47466864745962	21.946340439729997	27.814439557884597	31.76455135492579
51	18.83761272176351	22.90548507535921	31.01994229732729	27.236959905549995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2263.0
1	1920.0
2	1577.0
3	1154.0
4	731.0
5	706.0
6	681.0
7	730.5
8	780.0
9	869.0
10	958.0
11	1059.0
12	1160.0
13	1295.0
14	1430.0
15	1549.5
16	1669.0
17	1884.0
18	2099.0
19	2101.0
20	2103.0
21	1817.5
22	1532.0
23	2025.5
24	2519.0
25	2704.5
26	3252.5
27	3615.0
28	4418.5
29	5222.0
30	6228.5
31	7235.0
32	8784.0
33	10333.0
34	11376.5
35	12420.0
36	13596.0
37	14772.0
38	16301.5
39	17831.0
40	21230.0
41	24629.0
42	31359.0
43	38089.0
44	48770.5
45	59452.0
46	61796.0
47	64140.0
48	66798.5
49	69457.0
50	63843.5
51	58230.0
52	54342.0
53	50454.0
54	44580.5
55	38707.0
56	36164.0
57	33621.0
58	31980.5
59	30340.0
60	29250.0
61	28160.0
62	25285.5
63	22411.0
64	19236.0
65	16061.0
66	14310.5
67	12560.0
68	9883.5
69	7207.0
70	6555.5
71	5904.0
72	4680.0
73	3456.0
74	3023.5
75	2043.5
76	1496.0
77	1091.5
78	687.0
79	586.0
80	485.0
81	348.0
82	211.0
83	151.0
84	91.0
85	70.0
86	49.0
87	33.0
88	17.0
89	15.5
90	14.0
91	11.0
92	8.0
93	8.0
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	662361.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.025167132377522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27848585890109	23.803497879153333
2	9.382579454718725	5.634270325214825
3	3.3219151763574795	2.99223175109144
4	1.5789374733205377	1.8963144611209206
5	0.8674020391484679	1.302194559819891
6	0.5320956520848373	0.9585756530555184
7	0.3732657462164461	0.7845156490458283
8	0.28730700177100876	0.6901152597181459
9	0.23946520142597766	0.6470984425683084
>10	2.6719967168186436	19.95946147408159
>50	1.1604567431210215	25.033069920110172
>100	0.29810356094802354	11.844339708320312
>500	0.004993359479866391	1.1064572226605218
>1k	0.0024966797399331954	2.2796317168771325
>5k	4.993359479866391E-4	1.0682259771620894
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	7125	1.0756973916036723	TruSeq Adapter, Index 27 (100% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	4356	0.6576474158351714	No Hit
AATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	3826	0.5776306274071088	TruSeq Adapter, Index 27 (100% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGT	3608	0.5447180616008491	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1893	0.2857958122534388	No Hit
AAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	1522	0.22978406035379498	TruSeq Adapter, Index 27 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	966	0.14584192004058208	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	906	0.1367834156902354	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	874	0.13195221337005048	No Hit
AATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	871	0.13149928815253314	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	826	0.12470540988977309	TruSeq Adapter, Index 27 (96% over 25bp)
AATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	688	0.10387084988397566	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5097507250577856E-4	0.0	0.0	1.6059218462439666	0.0
2	1.5097507250577856E-4	0.0	0.0	1.9853222034509883	0.0
3	1.5097507250577856E-4	0.0	0.0	2.5176603091063634	0.0
4	1.5097507250577856E-4	0.0	0.0	5.33455321191918	0.0
5	1.5097507250577856E-4	0.0	0.0	5.630766304175517	0.0
6	3.019501450115571E-4	0.0	0.0	7.337539498853344	0.0
7	3.019501450115571E-4	0.0	0.0	8.675782541544566	0.0
8	3.019501450115571E-4	0.0	0.0	9.400613864644809	0.0
9	3.019501450115571E-4	0.0	0.0	11.343059147504155	0.0
10	3.019501450115571E-4	0.0	0.0	13.211073719618154	0.0
11	3.019501450115571E-4	0.0	0.0	15.955951512845713	0.0
12	3.019501450115571E-4	0.0	0.0	16.932307306740583	0.0
13	3.019501450115571E-4	0.0	0.0	17.299176732929627	0.0
14	3.019501450115571E-4	0.0	0.0	17.74742172319928	0.0
15	3.019501450115571E-4	0.0	0.0	18.08696466126478	0.0
16	3.019501450115571E-4	0.0	0.0	18.717134613903898	0.0
17	3.019501450115571E-4	0.0	0.0	19.470047300490215	0.0
18	3.019501450115571E-4	0.0	0.0	20.363215829434402	0.0
19	3.019501450115571E-4	0.0	0.0	20.80783741796392	0.0
20	3.019501450115571E-4	0.0	0.0	21.198258955463864	0.0
21	3.019501450115571E-4	0.0	0.0	21.686361364875044	0.0
22	3.019501450115571E-4	0.0	0.0	22.161932843268247	0.0
23	3.019501450115571E-4	0.0	0.0	22.627388991803564	0.0
24	3.019501450115571E-4	0.0	0.0	22.971461182044234	0.0
25	4.529252175173357E-4	0.0	0.0	23.277638629085953	0.0
26	4.529252175173357E-4	0.0	0.0	23.557848363656678	0.0
27	4.529252175173357E-4	0.0	0.0	23.843040275620094	0.0
28	4.529252175173357E-4	0.0	0.0	24.116456131928057	0.0
29	4.529252175173357E-4	0.0	0.0	24.406630221284164	0.0
30	4.529252175173357E-4	0.0	0.0	24.749343635872282	0.0
31	6.039002900231142E-4	0.0	0.0	25.05869155943662	0.0
32	6.039002900231142E-4	0.0	0.0	25.334221066759667	0.0
33	6.039002900231142E-4	0.0	0.0	25.607334972922622	0.0
34	6.039002900231142E-4	0.0	0.0	25.8706354993727	0.0
35	7.548753625288929E-4	0.0	0.0	26.199761157435297	0.0
36	7.548753625288929E-4	0.0	0.0	26.485556969688734	0.0
37	7.548753625288929E-4	0.0	0.0	26.759123801069205	0.0
38	7.548753625288929E-4	0.0	0.0	27.060319070718233	0.0
39	7.548753625288929E-4	0.0	0.0	27.35940068935218	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCA	25	3.8890084E-5	45.000004	34
ACGTTAG	50	2.1827873E-11	45.000004	1
ATAGCCG	25	3.8890084E-5	45.000004	1
ATAGACG	25	3.8890084E-5	45.000004	1
GGTCAAT	25	3.8890084E-5	45.000004	8
TCTAGCG	25	3.8890084E-5	45.000004	1
GCGCAAA	25	3.8890084E-5	45.000004	25
CCCTTGT	25	3.8890084E-5	45.000004	15
CATACCG	45	3.8380676E-10	45.000004	1
CTCGACT	25	3.8890084E-5	45.000004	22
TATAGCG	25	3.8890084E-5	45.000004	1
TTAAACG	25	3.8890084E-5	45.000004	1
TTCGGGC	25	3.8890084E-5	45.000004	4
GTGCGGC	25	3.8890084E-5	45.000004	21
ATCGACG	25	3.8890084E-5	45.000004	1
GGTAGAC	25	3.8890084E-5	45.000004	9
AGGTACG	20	7.031119E-4	45.0	11
TACCCTT	20	7.031119E-4	45.0	32
GTCGCAT	20	7.031119E-4	45.0	42
ACAGTCG	20	7.031119E-4	45.0	1
>>END_MODULE