FastQCFastQC Report
Sat 18 Jun 2016
SRR3552932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674850
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT66780.9895532340520116TruSeq Adapter, Index 27 (100% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC40500.6001333629695488No Hit
AATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT36870.546343631918204TruSeq Adapter, Index 27 (100% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGT35660.5284137215677558No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19610.29058309253908277No Hit
AAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT14010.20760168926428094TruSeq Adapter, Index 27 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9440.1398829369489516No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA8730.12936208046232497No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT8100.12002667259390977No Hit
AATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT7860.1164703267392754No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC6770.10031858931614432TruSeq Adapter, Index 27 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT207.031215E-445.026
GTCGCAT207.031215E-445.042
TCCGCGT453.8380676E-1045.022
TCACCGT253.889088E-545.026
TCACCGC253.889088E-545.024
CATATCG207.031215E-445.041
CGTATCT207.031215E-445.017
CGAAATG351.2109376E-745.01
CGAAACG351.2109376E-745.01
CGACGGT253.889088E-545.028
ACGTTAG502.1827873E-1145.01
CATGCAA207.031215E-445.026
TAGCGTA207.031215E-445.022
CGTGACG207.031215E-445.018
GGCGTGA253.889088E-545.018
GGCCGTA207.031215E-445.019
TAGAACG453.8380676E-1045.01
TAGCGAA207.031215E-445.035
CGACCTT207.031215E-445.025
TTCGTCG253.889088E-545.045
Produced by FastQC (version 0.11.5)