##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552932_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	674850
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10881677409795	31.0	31.0	34.0	28.0	34.0
2	31.321535155960582	31.0	31.0	34.0	28.0	34.0
3	31.330080758687117	31.0	31.0	34.0	28.0	34.0
4	35.33576350300066	37.0	35.0	37.0	33.0	37.0
5	34.9848203304438	37.0	35.0	37.0	32.0	37.0
6	35.04718826405868	36.0	35.0	37.0	32.0	37.0
7	35.005253019189446	37.0	35.0	37.0	32.0	37.0
8	35.36536119137586	37.0	35.0	37.0	33.0	37.0
9	35.79349040527524	39.0	37.0	39.0	32.0	39.0
10	35.665952433874196	38.0	35.0	39.0	30.0	39.0
11	36.089062754686225	38.0	35.0	39.0	31.0	39.0
12	35.760709787360156	37.0	35.0	39.0	31.0	39.0
13	35.4481662591687	37.0	35.0	39.0	30.0	39.0
14	36.44052011558124	38.0	35.0	40.0	31.0	41.0
15	36.50395939838483	38.0	35.0	40.0	31.0	41.0
16	36.55469363562273	38.0	35.0	40.0	31.0	41.0
17	36.54617618730088	38.0	35.0	40.0	31.0	41.0
18	36.412432392383494	38.0	35.0	40.0	31.0	41.0
19	36.42218863451137	38.0	35.0	40.0	31.0	41.0
20	36.36878713788249	38.0	35.0	40.0	30.0	41.0
21	36.34833074016448	38.0	35.0	40.0	30.0	41.0
22	36.35053122916204	38.0	35.0	40.0	30.0	41.0
23	36.31145587908424	38.0	35.0	40.0	31.0	41.0
24	35.91738608579684	38.0	34.0	40.0	30.0	41.0
25	36.09608209231681	38.0	34.0	40.0	30.0	41.0
26	35.89938208490776	38.0	34.0	40.0	30.0	41.0
27	35.866595539749575	38.0	34.0	40.0	30.0	41.0
28	35.815753130325255	38.0	34.0	40.0	30.0	41.0
29	35.52931910794992	38.0	34.0	40.0	29.0	41.0
30	35.60870563828999	38.0	34.0	40.0	29.0	41.0
31	35.45295547158628	38.0	34.0	40.0	29.0	41.0
32	35.40691116544417	38.0	34.0	40.0	29.0	41.0
33	35.36365118174409	38.0	34.0	40.0	28.0	41.0
34	35.28541157294214	38.0	34.0	40.0	27.0	41.0
35	35.16669778469289	37.0	34.0	40.0	27.0	41.0
36	34.9586233977921	37.0	34.0	40.0	26.0	41.0
37	34.79232570200785	37.0	33.0	40.0	26.0	41.0
38	34.7366540712751	37.0	33.0	40.0	26.0	41.0
39	34.59843965325628	37.0	33.0	40.0	25.0	41.0
40	34.41669704378751	37.0	33.0	40.0	24.0	41.0
41	34.52335926502186	37.0	33.0	40.0	25.0	41.0
42	34.41344298733052	37.0	33.0	40.0	24.0	41.0
43	34.269754760317106	36.0	33.0	40.0	24.0	41.0
44	34.20244202415351	36.0	33.0	40.0	24.0	41.0
45	33.86027709861451	36.0	33.0	40.0	23.0	41.0
46	33.72902719122768	36.0	33.0	39.0	23.0	41.0
47	33.571008372230864	35.0	32.0	39.0	22.0	41.0
48	33.502033044380234	35.0	32.0	39.0	22.0	41.0
49	33.37952285693117	35.0	32.0	39.0	22.0	40.0
50	33.2490153367415	35.0	32.0	39.0	22.0	40.0
51	31.40955175224124	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	13.0
11	19.0
12	13.0
13	11.0
14	47.0
15	74.0
16	126.0
17	271.0
18	672.0
19	1117.0
20	1892.0
21	2639.0
22	3917.0
23	4995.0
24	6100.0
25	7344.0
26	8445.0
27	10836.0
28	13113.0
29	16262.0
30	20555.0
31	25621.0
32	33630.0
33	45473.0
34	70456.0
35	72486.0
36	68526.0
37	88141.0
38	102973.0
39	69067.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.89042009335408	34.04830703119212	20.79069422834704	26.270578647106763
2	20.842853967548344	33.87419426539231	22.00296362154553	23.27998814551382
3	20.82181225457509	31.00377861747055	22.467066755575313	25.70734237237905
4	21.04067570571238	31.249907386826703	20.06149514706972	27.6479217603912
5	17.824701785581983	36.400681632955475	19.07179373194043	26.702822849522118
6	21.099948136622952	36.173816403645255	21.146625175965028	21.579610283766762
7	78.12713936430318	8.805512336074683	6.929984440986886	6.137363858635252
8	80.58709342816923	5.973623768244795	5.956731125435282	7.482551678150701
9	72.9625842779877	11.934059420611987	9.879084240942431	5.22427206045788
10	43.12336074683263	27.09387271245462	13.260576424390605	16.522190116322147
11	35.260280062236056	23.658442616877824	23.116099874046085	17.965177446840038
12	33.955990220048896	22.52574646217678	26.420834259465064	17.097429058309256
13	24.82625768689338	31.118174409127953	22.33474105356746	21.7208268504112
14	18.512706527376455	32.30466029488034	28.977698747869894	20.204934429873305
15	16.928354449136844	28.084018670815738	32.515521967844705	22.472104912202713
16	18.716010965399718	24.797214195747202	33.220863895680516	23.265910943172557
17	18.537749129436172	26.158701933763055	27.502259761428466	27.801289175372307
18	21.586130251166928	26.605764243906055	26.38334444691413	25.42476105801289
19	23.107505371564052	30.390901681855226	26.697488330740164	19.804104615840558
20	27.60420834259465	27.737867674297995	23.986367340890567	20.67155664221679
21	21.810920945395274	31.006297695784248	24.19693265170038	22.985848707120102
22	21.33926057642439	28.234718826405867	25.294658072164182	25.131362525005557
23	23.547899533229607	28.290434911461805	21.685559754019412	26.476105801289172
24	19.575313032525745	29.581240275616803	27.41453656368082	23.428910128176632
25	19.92976216937097	26.057938801215087	26.04578795287842	27.96651107653553
26	21.78232199748092	33.0738682670223	21.748536711861895	23.395273023634882
27	18.134548418167	29.47929169445062	25.8522634659554	26.533896421426984
28	19.275839075350078	26.704749203526713	27.200118544861823	26.819293176261393
29	19.937023042157517	29.17359413202934	27.23716381418093	23.652219011632212
30	21.514262428687854	26.9028673038453	24.291472179002742	27.291398088464103
31	24.14195747203082	28.523375564940356	21.193005853152552	26.141661109876267
32	24.604578795287843	25.449507297918057	23.76291027635771	26.183003630436392
33	23.6039119804401	25.084389123508927	25.633400014818108	25.678298881232863
34	18.48973846039861	26.161517374231313	26.744906275468622	28.603837889901463
35	19.94606208787138	24.834555827220864	26.206712602800625	29.012669482107135
36	23.437949173890495	25.985922797658738	27.705564199451732	22.87056382899904
37	19.334963325183374	28.13425205601245	24.884789212417573	27.645995406386604
38	21.888123286656295	25.18574498036601	27.828850855745724	25.097280877231974
39	21.008816774097948	24.402311624805513	30.32673927539453	24.26213232570201
40	22.249833296288063	24.106838556716305	26.099429502852484	27.543898644143145
41	18.444987775061126	21.36252500555679	30.583685263391864	29.60880195599022
42	22.9003482255316	22.8305549381344	24.534785507890643	29.734311328443358
43	20.986885974661035	24.10757946210269	26.997703193302215	27.907831369934062
44	21.545824998147737	22.95517522412388	25.837000814995925	29.66199896273246
45	22.84715121878936	26.333259242794693	24.79365784989257	26.025931688523375
46	18.741201748536714	24.698229236126544	31.752241238793804	24.808327776542935
47	21.032970289694006	24.343335556049492	26.581907090464547	28.041787063791958
48	21.30443802326443	20.954137956582944	32.98940505297473	24.75201896717789
49	21.001555901311402	21.106912647254948	29.78854560272653	28.102985848707117
50	18.65066311032081	22.385418981996	27.671334370600874	31.292583537082315
51	18.90360820923168	23.533822330888345	30.514188338149218	27.048381121730753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2341.0
1	2049.5
2	1758.0
3	1259.0
4	760.0
5	741.0
6	722.0
7	788.5
8	855.0
9	906.0
10	957.0
11	1051.0
12	1145.0
13	1240.5
14	1336.0
15	1512.5
16	1689.0
17	1901.0
18	2113.0
19	2095.5
20	2078.0
21	1854.5
22	1631.0
23	2181.5
24	2732.0
25	2937.0
26	3503.0
27	3864.0
28	4575.0
29	5286.0
30	6502.0
31	7718.0
32	9237.5
33	10757.0
34	11771.5
35	12786.0
36	14158.0
37	15530.0
38	17091.0
39	18652.0
40	22144.0
41	25636.0
42	32331.0
43	39026.0
44	49654.5
45	60283.0
46	62040.5
47	63798.0
48	66673.5
49	69549.0
50	63601.5
51	57654.0
52	54225.5
53	50797.0
54	45014.0
55	39231.0
56	37047.0
57	34863.0
58	33114.0
59	31365.0
60	30146.0
61	28927.0
62	26222.0
63	23517.0
64	20145.0
65	16773.0
66	14784.5
67	12796.0
68	10087.5
69	7379.0
70	6821.0
71	6263.0
72	4909.5
73	3556.0
74	3086.0
75	2025.5
76	1435.0
77	1038.0
78	641.0
79	559.0
80	477.0
81	358.0
82	239.0
83	155.5
84	72.0
85	59.5
86	47.0
87	32.0
88	17.0
89	14.5
90	12.0
91	11.5
92	11.0
93	8.5
94	6.0
95	5.5
96	5.0
97	4.0
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	674850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.472558261932026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45441460132604	23.122523052433525
2	9.690624983646224	5.7121501885009485
3	3.54429443332369	3.1337827255072144
4	1.717022896538527	2.024202294212121
5	0.9377848709674542	1.3819459623373351
6	0.5562014759353697	0.9835608242926659
7	0.4141101344514962	0.8543419545134758
8	0.28140009356307805	0.6634864521960753
9	0.22037857049688392	0.5845608232785636
>10	2.691477106090356	19.888411573938058
>50	1.1441064840529664	24.37979267023132
>100	0.34060413880373897	13.127168222669553
>500	0.004548126482542508	0.965272382526508
>1k	0.002526736934745838	2.184187546636513
>5k	5.053473869491675E-4	0.9946133267261258
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	6678	0.9895532340520116	TruSeq Adapter, Index 27 (100% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	4050	0.6001333629695488	No Hit
AATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	3687	0.546343631918204	TruSeq Adapter, Index 27 (100% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGT	3566	0.5284137215677558	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1961	0.29058309253908277	No Hit
AAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	1401	0.20760168926428094	TruSeq Adapter, Index 27 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	944	0.1398829369489516	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	873	0.12936208046232497	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	810	0.12002667259390977	No Hit
AATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	786	0.1164703267392754	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	677	0.10031858931614432	TruSeq Adapter, Index 27 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.963621545528636E-4	0.0	0.0	1.4555827220863895	0.0
2	2.963621545528636E-4	0.0	0.0	1.7737274949988886	0.0
3	2.963621545528636E-4	0.0	0.0	2.2496851152107875	0.0
4	2.963621545528636E-4	0.0	0.0	4.765651626287323	0.0
5	2.963621545528636E-4	0.0	0.0	5.052974735126324	0.0
6	2.963621545528636E-4	0.0	0.0	6.550196339927392	0.0
7	2.963621545528636E-4	0.0	0.0	7.7720975031488475	0.0
8	2.963621545528636E-4	0.0	0.0	8.436245091501815	0.0
9	2.963621545528636E-4	0.0	0.0	10.2458324072016	0.0
10	2.963621545528636E-4	0.0	0.0	12.001333629695488	0.0
11	2.963621545528636E-4	0.0	0.0	14.501592946580722	0.0
12	2.963621545528636E-4	0.0	0.0	15.422834704008299	0.0
13	2.963621545528636E-4	0.0	0.0	15.772245684226124	0.0
14	2.963621545528636E-4	0.0	0.0	16.19885900570497	0.0
15	2.963621545528636E-4	0.0	0.0	16.51033563014003	0.0
16	2.963621545528636E-4	0.0	0.0	17.099799955545677	0.0
17	2.963621545528636E-4	0.0	0.0	17.801437356449583	0.0
18	2.963621545528636E-4	0.0	0.0	18.669333926057643	0.0
19	2.963621545528636E-4	0.0	0.0	19.101133585241165	0.0
20	2.963621545528636E-4	0.0	0.0	19.486848929391716	0.0
21	2.963621545528636E-4	0.0	0.0	19.949618433726013	0.0
22	2.963621545528636E-4	0.0	0.0	20.402311624805513	0.0
23	2.963621545528636E-4	0.0	0.0	20.85263391864859	0.0
24	2.963621545528636E-4	0.0	0.0	21.204563977180115	0.0
25	2.963621545528636E-4	0.0	0.0	21.475587167518707	0.0
26	2.963621545528636E-4	0.0	0.0	21.752685782025637	0.0
27	2.963621545528636E-4	0.0	0.0	22.029339853300733	0.0
28	2.963621545528636E-4	0.0	0.0	22.28613766022079	0.0
29	2.963621545528636E-4	0.0	0.0	22.555975401941172	0.0
30	2.963621545528636E-4	0.0	0.0	22.885530117803956	0.0
31	2.963621545528636E-4	0.0	0.0	23.195524931466252	0.0
32	2.963621545528636E-4	0.0	0.0	23.459879973327407	0.0
33	2.963621545528636E-4	0.0	0.0	23.717863228865674	0.0
34	2.963621545528636E-4	0.0	0.0	23.98473734904053	0.0
35	2.963621545528636E-4	0.0	0.0	24.29562124916648	0.0
36	2.963621545528636E-4	0.0	0.0	24.57183077720975	0.0
37	2.963621545528636E-4	0.0	0.0	24.83440764614359	0.0
38	4.445432318292954E-4	0.0	0.0	25.140994295028523	0.0
39	4.445432318292954E-4	0.0	0.0	25.438986441431428	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGAT	20	7.031215E-4	45.0	26
GTCGCAT	20	7.031215E-4	45.0	42
TCCGCGT	45	3.8380676E-10	45.0	22
TCACCGT	25	3.889088E-5	45.0	26
TCACCGC	25	3.889088E-5	45.0	24
CATATCG	20	7.031215E-4	45.0	41
CGTATCT	20	7.031215E-4	45.0	17
CGAAATG	35	1.2109376E-7	45.0	1
CGAAACG	35	1.2109376E-7	45.0	1
CGACGGT	25	3.889088E-5	45.0	28
ACGTTAG	50	2.1827873E-11	45.0	1
CATGCAA	20	7.031215E-4	45.0	26
TAGCGTA	20	7.031215E-4	45.0	22
CGTGACG	20	7.031215E-4	45.0	18
GGCGTGA	25	3.889088E-5	45.0	18
GGCCGTA	20	7.031215E-4	45.0	19
TAGAACG	45	3.8380676E-10	45.0	1
TAGCGAA	20	7.031215E-4	45.0	35
CGACCTT	20	7.031215E-4	45.0	25
TTCGTCG	25	3.889088E-5	45.0	45
>>END_MODULE