FastQCFastQC Report
Sat 18 Jun 2016
SRR3552928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137421
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC146071.2842210579899613No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGT117391.0320716779451056No Hit
AATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT106670.9378233741068611No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT68510.6023275462647516No Hit
AATGACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT24560.21592708416672454No Hit
AATGATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT21330.1875295075438206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21220.18656240741115207No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGCGAATCGTAT15370.13513026399196076No Hit
AACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG15280.13433900024705014No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG14820.13029476332861797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTA302.1654814E-645.00000418
ACTATCG351.2119199E-745.0000041
TACCACG600.045.0000041
TCGATAC302.1654814E-645.00000424
GTTACGT351.2119199E-745.00000445
CGTAGAC302.1654814E-645.00000419
GTATCGC351.2119199E-745.00000429
CTCGATA302.1654814E-645.00000423
TACTACG600.045.0000041
CGGTCTA351.2119199E-745.00000431
TTCGAAC351.2119199E-745.00000410
TCGAACA351.2119199E-745.00000411
TACGTCG351.2119199E-745.0000041
AATCGCG600.045.0000041
AGTAACG302.1654814E-645.0000041
AGTCGGT351.2119199E-745.00000414
CCCGATA351.2119199E-745.00000442
AACCGTA207.033257E-445.017
AAGTACC207.033257E-445.020
GTCGATT253.8907812E-545.011
Produced by FastQC (version 0.11.5)