##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552928_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137421
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298696788612133	31.0	31.0	34.0	30.0	34.0
2	31.522220883911938	31.0	31.0	34.0	30.0	34.0
3	31.47202135357093	31.0	31.0	34.0	28.0	34.0
4	35.45554636321995	37.0	35.0	37.0	33.0	37.0
5	35.13827685615089	37.0	35.0	37.0	32.0	37.0
6	35.20359040320163	37.0	35.0	37.0	32.0	37.0
7	35.12541618275027	37.0	35.0	37.0	32.0	37.0
8	35.510722063334505	37.0	35.0	37.0	33.0	37.0
9	35.998008652908645	39.0	37.0	39.0	32.0	39.0
10	35.85948650499683	38.0	35.0	39.0	30.0	39.0
11	36.24638634243609	38.0	35.0	39.0	32.0	39.0
12	35.90905390352385	37.0	35.0	39.0	31.0	39.0
13	35.703086192359734	37.0	35.0	39.0	30.0	39.0
14	36.74751213490871	38.0	35.0	40.0	31.0	41.0
15	36.85810179344324	38.0	35.0	40.0	32.0	41.0
16	36.86222691510004	38.0	35.0	40.0	32.0	41.0
17	36.85243986175743	38.0	35.0	40.0	32.0	41.0
18	36.683274706551046	38.0	35.0	40.0	31.0	41.0
19	36.73175543620172	38.0	35.0	40.0	31.0	41.0
20	36.71879541524203	38.0	35.0	40.0	31.0	41.0
21	36.753230334238594	38.0	35.0	40.0	31.0	41.0
22	36.72386917421078	38.0	35.0	40.0	31.0	41.0
23	36.66710391315089	38.0	35.0	40.0	31.0	41.0
24	36.30706044639584	38.0	35.0	40.0	30.0	41.0
25	36.370225272788176	38.0	35.0	40.0	30.0	41.0
26	36.304282231469266	38.0	35.0	40.0	30.0	41.0
27	36.190207495729375	38.0	35.0	40.0	30.0	41.0
28	36.11585332080206	38.0	35.0	40.0	30.0	41.0
29	35.93415366869435	38.0	35.0	40.0	30.0	41.0
30	35.937663362994	38.0	35.0	40.0	30.0	41.0
31	35.875782142232296	38.0	34.0	40.0	30.0	41.0
32	35.80239242989183	38.0	34.0	40.0	30.0	41.0
33	35.73506028110963	38.0	34.0	40.0	30.0	41.0
34	35.61849921884685	38.0	34.0	40.0	29.0	41.0
35	35.46020866504135	38.0	34.0	40.0	28.0	41.0
36	35.38990751885186	38.0	34.0	40.0	28.0	41.0
37	35.1966088194257	37.0	34.0	40.0	27.0	41.0
38	35.22351618266236	37.0	34.0	40.0	28.0	41.0
39	35.04646212791921	37.0	34.0	40.0	27.0	41.0
40	34.846366472924274	37.0	33.0	40.0	26.0	41.0
41	34.8349415036297	37.0	33.0	40.0	26.0	41.0
42	34.85212511462335	37.0	33.0	40.0	26.0	41.0
43	34.837777744564235	37.0	33.0	40.0	27.0	41.0
44	34.78395158872572	37.0	33.0	40.0	26.0	41.0
45	34.57958047196245	36.0	33.0	40.0	26.0	41.0
46	34.33811842756552	36.0	33.0	40.0	25.0	41.0
47	34.229016344871425	36.0	33.0	40.0	25.0	41.0
48	34.12455458445026	36.0	33.0	39.0	24.0	41.0
49	34.013647541235834	36.0	33.0	39.0	24.0	41.0
50	33.88525532762275	35.0	33.0	39.0	24.0	40.0
51	31.930900695520833	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	9.0
10	18.0
11	17.0
12	28.0
13	25.0
14	59.0
15	81.0
16	208.0
17	464.0
18	812.0
19	1443.0
20	2355.0
21	3497.0
22	4808.0
23	6451.0
24	7894.0
25	9553.0
26	11780.0
27	14750.0
28	18755.0
29	23976.0
30	30881.0
31	40604.0
32	53193.0
33	72476.0
34	115594.0
35	124579.0
36	118674.0
37	154140.0
38	187822.0
39	132461.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.260590405839174	35.379951662577	20.380931950438754	24.978525981145065
2	22.361289267562316	34.95108671283544	19.80014436167435	22.88747965792789
3	20.14909167317994	30.641864358052118	24.05626412735478	25.152779841413164
4	23.487169658376274	30.95775442865922	18.299117037578874	27.255958875385634
5	18.98224140401839	35.616627440499165	18.122401467882167	27.278729687600283
6	20.8859340560795	35.638343234387264	21.616358410825896	21.85936429870734
7	79.86638192894276	9.405224626589451	5.932807641146066	4.795585803321726
8	82.05774291137583	4.709162218738708	4.752769642902672	8.480325226982798
9	75.88166562776667	10.182861051448848	8.29763122010232	5.637842100682158
10	45.295101813664424	28.020055898387668	12.325603272666848	14.35923901528106
11	35.92363777352449	24.380154753604867	21.43348856755766	18.262718905312987
12	33.378406060728615	23.115363616462155	26.33747750393214	17.168752818877092
13	25.748425604943115	30.556583709989525	22.599987163943695	21.095003521123665
14	19.73068898850997	31.048222250160673	29.67889638049588	19.54219238083348
15	18.726751132606132	26.56263599845616	30.97226093064925	23.73835193828846
16	20.147333309302358	24.316150308460983	32.96308051284441	22.57343586939225
17	20.07365786283179	24.78686431848893	27.741355223791363	27.39812259488791
18	23.06314020929805	26.161816952561978	26.202698912715693	24.572343925424274
19	24.414267012829903	28.6730243243267	26.13051807554107	20.782190587302328
20	28.98636476731131	28.364431463811552	22.061488226435067	20.587715542442066
21	24.099695715130984	26.926793157502804	24.676175312395323	24.297335814970886
22	23.071316601328796	27.01093086904497	24.73297046564113	25.1847820639851
23	24.65111862713982	27.830943863354022	21.722915261807195	25.795022247698963
24	22.287262148316234	28.83118915511495	26.331674903136133	22.54987379343269
25	20.98607287890763	26.563778934976582	25.881006241312583	26.5691419448032
26	24.07041895656929	30.725650396818764	21.90569718688155	23.29823345973039
27	21.356911820689085	28.70432320134761	25.864653457251098	24.074111520712208
28	22.63058269541357	28.261391340585412	26.433396253454085	22.67462971054693
29	20.672468681341385	28.182352884288225	26.58760476551778	24.557573668852605
30	24.810250558060737	28.2405547286361	23.29884888708754	23.650345826215624
31	28.642516711050703	28.2844259073817	19.425173264780586	23.64788411678701
32	25.48423143233684	26.811884078103006	21.248157014860812	26.45572747469934
33	28.14507557008355	28.14841646145095	21.39454080766928	22.31196716079622
34	20.998733098826207	29.656389322862864	22.030541022189674	27.314336556121262
35	22.63831949647492	30.63289670227646	24.08633214966138	22.64245165158723
36	26.916506728818966	27.0810016695665	23.350281030506732	22.652210571107794
37	22.826200676794254	31.821286928938363	20.99820558966293	24.35430680460445
38	27.630578299503878	28.017066679795782	21.779710415053003	22.572644605647337
39	23.37331559730302	26.33000445745243	24.18031669891799	26.116363246326557
40	25.880039141179918	28.541059115314383	22.43962437830847	23.13927736519723
41	20.995040534683287	25.988266437844914	27.10245370887297	25.91423931859883
42	25.23428000713896	27.15089663370027	21.852067088615385	25.762756270545385
43	24.239573561592408	24.056352045548657	25.765921325525028	25.938153067333907
44	22.098501786058108	24.754598341335353	25.749128950494143	27.39777092211239
45	25.911953445557977	27.936709450590413	23.184379398657136	22.96695770519447
46	21.5093619688752	25.809440831495113	29.116307857864417	23.564889341765273
47	23.353446085486375	27.56129876272726	24.13969849334591	24.945556658440456
48	23.59636405517394	23.562075959561145	29.239481247488836	23.602078737776075
49	23.535172992234184	22.841674278916955	27.228440480701515	26.394712248147346
50	21.579872360366124	25.659979901900883	24.067165983395768	28.692981754337225
51	20.530480798226865	25.502430498469785	27.858813930813657	26.108274772489693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2591.0
1	2167.5
2	1744.0
3	1266.0
4	788.0
5	805.5
6	823.0
7	844.5
8	866.0
9	950.0
10	1034.0
11	1147.0
12	1260.0
13	1454.0
14	1648.0
15	1737.5
16	1827.0
17	2004.0
18	2181.0
19	2476.5
20	2772.0
21	2557.0
22	2342.0
23	3362.0
24	4382.0
25	4617.0
26	5409.0
27	5966.0
28	7514.5
29	9063.0
30	10145.0
31	11227.0
32	13330.0
33	15433.0
34	17976.5
35	20520.0
36	22778.0
37	25036.0
38	28495.5
39	31955.0
40	37890.5
41	43826.0
42	53587.0
43	63348.0
44	84103.0
45	104858.0
46	111387.5
47	117917.0
48	111775.5
49	105634.0
50	98156.5
51	90679.0
52	84304.5
53	77930.0
54	71793.0
55	65656.0
56	62362.5
57	59069.0
58	57812.0
59	56555.0
60	53299.5
61	50044.0
62	47272.0
63	44500.0
64	39755.5
65	35011.0
66	29336.0
67	23661.0
68	20645.0
69	17629.0
70	14897.0
71	12165.0
72	9990.0
73	7815.0
74	6611.5
75	4625.0
76	3842.0
77	2954.5
78	2067.0
79	1371.0
80	675.0
81	508.0
82	341.0
83	282.5
84	224.0
85	180.0
86	136.0
87	92.5
88	49.0
89	46.0
90	43.0
91	31.5
92	20.0
93	12.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1137421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.945924963678372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18830360634009	17.71157022624704
2	10.02143944721048	4.599023951674762
3	3.7992918568325944	2.6153479758598492
4	1.9406804938363102	1.7812283596016905
5	1.108117300767093	1.2713388217177768
6	0.686954647349084	0.9457685874913333
7	0.4610539354050846	0.7405516304209581
8	0.3244363292537455	0.5955593333238155
9	0.23756200574749273	0.4905971962292609
>10	2.184004490773556	12.178986199138466
>50	1.040430870062626	17.46034257937653
>100	1.0000295218329276	34.11106471196025
>500	0.0034629725650236042	0.5365390634824783
>1k	0.00269342310612947	1.0893235091734108
>5k	3.847747294470672E-4	0.6048756169028235
>10k+	0.0011543241883412016	3.267882237399534
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC	14607	1.2842210579899613	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGT	11739	1.0320716779451056	No Hit
AATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	10667	0.9378233741068611	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	6851	0.6023275462647516	No Hit
AATGACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT	2456	0.21592708416672454	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT	2133	0.1875295075438206	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2122	0.18656240741115207	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGCGAATCGTAT	1537	0.13513026399196076	No Hit
AACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	1528	0.13433900024705014	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1482	0.13029476332861797	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.791819387895951E-5	0.0	0.0	0.8435750702686164	0.0
2	8.791819387895951E-5	0.0	0.0	1.022488594812299	0.0
3	8.791819387895951E-5	0.0	0.0	1.411262848145058	0.0
4	8.791819387895951E-5	0.0	0.0	3.5073205084133314	0.0
5	8.791819387895951E-5	0.0	0.0	3.6959929524775785	0.0
6	8.791819387895951E-5	0.0	0.0	4.67355535021773	0.0
7	8.791819387895951E-5	0.0	0.0	5.466577459005944	0.0
8	8.791819387895951E-5	0.0	0.0	5.891486090022956	0.0
9	8.791819387895951E-5	0.0	0.0	7.8029155431454145	0.0
10	1.7583638775791902E-4	0.0	0.0	8.821623655620918	0.0
11	1.7583638775791902E-4	0.0	0.0	11.416001638795134	0.0
12	1.7583638775791902E-4	0.0	0.0	11.98755781720225	0.0
13	1.7583638775791902E-4	0.0	0.0	12.269863137747588	0.0
14	1.7583638775791902E-4	0.0	0.0	12.782162453480286	0.0
15	1.7583638775791902E-4	0.0	0.0	13.02675086885155	0.0
16	1.7583638775791902E-4	0.0	0.0	13.457286264276815	0.0
17	1.7583638775791902E-4	0.0	0.0	13.920878900600568	0.0
18	1.7583638775791902E-4	0.0	0.0	14.528833211273573	8.791819387895951E-5
19	3.5167277551583805E-4	0.0	0.0	14.816325705257771	8.791819387895951E-5
20	3.5167277551583805E-4	0.0	0.0	15.071464303894512	8.791819387895951E-5
21	3.5167277551583805E-4	0.0	0.0	15.404322585920253	8.791819387895951E-5
22	3.5167277551583805E-4	0.0	0.0	15.74667603288492	8.791819387895951E-5
23	3.5167277551583805E-4	0.0	0.0	16.113998246911216	8.791819387895951E-5
24	3.5167277551583805E-4	0.0	0.0	16.388566766395204	8.791819387895951E-5
25	4.395909693947975E-4	0.0	0.0	16.608186414704846	8.791819387895951E-5
26	4.395909693947975E-4	0.0	0.0	16.806793614677414	8.791819387895951E-5
27	4.395909693947975E-4	0.0	0.0	17.007247096721443	8.791819387895951E-5
28	4.395909693947975E-4	0.0	0.0	17.20620596946953	8.791819387895951E-5
29	4.395909693947975E-4	0.0	0.0	17.436903310207917	8.791819387895951E-5
30	4.395909693947975E-4	0.0	0.0	17.727648777365637	8.791819387895951E-5
31	4.395909693947975E-4	0.0	0.0	17.945949652767094	8.791819387895951E-5
32	4.395909693947975E-4	0.0	0.0	18.17181149284214	8.791819387895951E-5
33	4.395909693947975E-4	0.0	0.0	18.411828162131698	8.791819387895951E-5
34	4.395909693947975E-4	0.0	0.0	18.621776809114657	8.791819387895951E-5
35	4.395909693947975E-4	0.0	0.0	18.889575627669966	8.791819387895951E-5
36	4.395909693947975E-4	0.0	0.0	19.10805233945918	8.791819387895951E-5
37	4.395909693947975E-4	0.0	0.0	19.34551938112625	8.791819387895951E-5
38	4.395909693947975E-4	0.0	0.0	19.579997204201433	8.791819387895951E-5
39	4.395909693947975E-4	0.0	0.0	19.829772793011557	8.791819387895951E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTA	30	2.1654814E-6	45.000004	18
ACTATCG	35	1.2119199E-7	45.000004	1
TACCACG	60	0.0	45.000004	1
TCGATAC	30	2.1654814E-6	45.000004	24
GTTACGT	35	1.2119199E-7	45.000004	45
CGTAGAC	30	2.1654814E-6	45.000004	19
GTATCGC	35	1.2119199E-7	45.000004	29
CTCGATA	30	2.1654814E-6	45.000004	23
TACTACG	60	0.0	45.000004	1
CGGTCTA	35	1.2119199E-7	45.000004	31
TTCGAAC	35	1.2119199E-7	45.000004	10
TCGAACA	35	1.2119199E-7	45.000004	11
TACGTCG	35	1.2119199E-7	45.000004	1
AATCGCG	60	0.0	45.000004	1
AGTAACG	30	2.1654814E-6	45.000004	1
AGTCGGT	35	1.2119199E-7	45.000004	14
CCCGATA	35	1.2119199E-7	45.000004	42
AACCGTA	20	7.033257E-4	45.0	17
AAGTACC	20	7.033257E-4	45.0	20
GTCGATT	25	3.8907812E-5	45.0	11
>>END_MODULE