FastQCFastQC Report
Sat 18 Jun 2016
SRR3552921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617901
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCC72091.1666917515912745No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGT63191.0226557328763022No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT52330.8468994223993812No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT43100.6975227423163257Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18220.29486924280750476No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT12870.20828579335524625No Hit
AAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT10210.16523682596402983No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT9440.1527752827718356No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTAT7100.11490513852542722No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT7060.11425778563232622No Hit
AACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG6560.10616587446856374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG302.1638662E-645.0000041
CGACAAT302.1638662E-645.00000420
ACGGCAT302.1638662E-645.00000423
CATAGGT302.1638662E-645.00000426
AGCGATA302.1638662E-645.00000443
GCGATAC302.1638662E-645.00000444
AACCTTA302.1638662E-645.00000424
CGTGTAC302.1638662E-645.00000437
CTCGAAC302.1638662E-645.00000421
AGGATCG302.1638662E-645.00000445
CAATCTA302.1638662E-645.00000411
CGATAGT302.1638662E-645.00000430
TACACCG302.1638662E-645.0000041
TAGTACG650.045.0000041
TGATCGG207.030752E-445.016
CGTGGGA207.030752E-445.04
AGACTCG207.030752E-445.043
CCTTACG207.030752E-445.01
TACCCGT207.030752E-445.034
TTGGATC207.030752E-445.043