FastQCFastQC Report
Sat 18 Jun 2016
SRR3552919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608087
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT54010.8881952746893126TruSeq Adapter, Index 27 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC20890.3435363689735186No Hit
AATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT20410.3356427616443042No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGT19210.31590874332126817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15860.2608179421694593No Hit
AAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT11140.18319747009885098No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT6680.1098527019982338No Hit
GCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCTA6130.10080794360017563No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC6100.10031459314209974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCG302.163808E-645.0000041
CGAAACG302.163808E-645.0000041
TTTCGCG302.163808E-645.0000041
GAGTACG600.045.0000041
GGCGTAA351.2106648E-745.00000410
TAGCGCG351.2106648E-745.0000041
CGCTACG351.2106648E-745.0000041
CATCGCG351.2106648E-745.0000041
CGTAACG302.163808E-645.0000041
CTTTCGT302.163808E-645.00000414
CCGCGTC302.163808E-645.00000441
ACGTAAA302.163808E-645.00000425
CGCGTCA302.163808E-645.00000442
TGTCGCC351.2106648E-745.00000426
CGATAAG302.163808E-645.0000041
CCGTTGG351.2106648E-745.0000042
TGCGTAA302.163808E-645.00000412
ATTTCGG302.163808E-645.0000042
TCACGAC207.0306635E-445.025
TCGCCCG207.0306635E-445.02