##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552919_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	608087
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426999754969273	31.0	31.0	34.0	30.0	34.0
2	31.614734404780894	31.0	31.0	34.0	30.0	34.0
3	31.818743041702913	31.0	31.0	34.0	30.0	34.0
4	35.65752926801593	37.0	35.0	37.0	33.0	37.0
5	35.27576646104916	37.0	35.0	37.0	33.0	37.0
6	35.283362413602	37.0	35.0	37.0	32.0	37.0
7	35.347920610044284	37.0	35.0	37.0	33.0	37.0
8	35.65062400610439	37.0	35.0	37.0	35.0	37.0
9	36.520854746113635	39.0	37.0	39.0	33.0	39.0
10	36.11037400898227	38.0	35.0	39.0	31.0	39.0
11	36.37219345258162	38.0	35.0	39.0	32.0	39.0
12	36.078630196008135	37.0	35.0	39.0	32.0	39.0
13	35.83071336831736	37.0	35.0	39.0	31.0	39.0
14	36.877553705308614	39.0	35.0	40.0	31.0	41.0
15	36.91989797512527	39.0	35.0	40.0	32.0	41.0
16	36.97549034924279	38.0	35.0	40.0	32.0	41.0
17	36.975118691897706	38.0	35.0	40.0	32.0	41.0
18	36.86468383635894	38.0	35.0	40.0	32.0	41.0
19	36.91337752657103	39.0	35.0	40.0	32.0	41.0
20	36.850570724254915	38.0	35.0	40.0	31.0	41.0
21	36.88410046588728	39.0	35.0	40.0	31.0	41.0
22	36.88570056587297	39.0	35.0	40.0	31.0	41.0
23	36.798852795734824	38.0	35.0	40.0	31.0	41.0
24	36.454101140132906	38.0	35.0	40.0	31.0	41.0
25	36.59147128618109	38.0	35.0	40.0	31.0	41.0
26	36.47121217852051	38.0	35.0	40.0	31.0	41.0
27	36.28784861376744	38.0	35.0	40.0	30.0	41.0
28	36.34396558387204	38.0	35.0	40.0	30.0	41.0
29	36.092869934729734	38.0	35.0	40.0	30.0	41.0
30	36.02249678088826	38.0	35.0	40.0	30.0	41.0
31	35.90704948469545	38.0	35.0	40.0	30.0	41.0
32	35.77181554613073	38.0	34.0	40.0	29.0	41.0
33	35.857992359645905	38.0	35.0	40.0	30.0	41.0
34	35.717611131301936	38.0	34.0	40.0	29.0	41.0
35	35.66501010546188	38.0	34.0	40.0	29.0	41.0
36	35.426016343056176	38.0	34.0	40.0	28.0	41.0
37	35.26560015260974	38.0	34.0	40.0	27.0	41.0
38	35.26137871719014	38.0	34.0	40.0	27.0	41.0
39	35.22506318997117	38.0	34.0	40.0	27.0	41.0
40	35.150356774606266	37.0	34.0	40.0	27.0	41.0
41	35.20584718962912	37.0	34.0	40.0	27.0	41.0
42	35.16451264374999	37.0	34.0	40.0	27.0	41.0
43	35.06353531649254	37.0	34.0	40.0	27.0	41.0
44	34.871621988301015	37.0	34.0	40.0	26.0	41.0
45	34.628696222744445	37.0	33.0	40.0	26.0	41.0
46	34.49510678570665	37.0	33.0	40.0	25.0	41.0
47	34.40444541652757	36.0	33.0	40.0	25.0	41.0
48	34.396097926776925	36.0	33.0	40.0	25.0	41.0
49	34.26649805044344	36.0	33.0	40.0	24.0	41.0
50	34.05675010319247	36.0	33.0	39.0	24.0	41.0
51	32.09779028329828	35.0	30.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	1.0
10	6.0
11	8.0
12	7.0
13	12.0
14	21.0
15	47.0
16	97.0
17	195.0
18	415.0
19	774.0
20	1276.0
21	1885.0
22	2696.0
23	3521.0
24	4454.0
25	5162.0
26	6246.0
27	7702.0
28	9570.0
29	12039.0
30	15411.0
31	19914.0
32	25822.0
33	35890.0
34	58271.0
35	64501.0
36	60667.0
37	81530.0
38	107418.0
39	82524.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.015063633986586	32.99133183245161	21.10405254511279	25.88955198844902
2	23.37757590607923	30.806776004091518	22.4652064589442	23.350441630885054
3	21.262582492307843	30.927317966014733	21.39529376553026	26.414805776147166
4	19.828577160833895	31.70286488611005	20.68519800620635	27.78335994684971
5	17.422342526645036	34.289007987343915	19.360552026272558	28.92809745973849
6	20.166357774463194	36.82219813941097	20.919210573487018	22.092233512638817
7	82.78733141803723	6.2982763979496355	5.897675825992004	5.016716358021139
8	85.42363181584214	4.6018086227793065	4.7152792281367635	5.259280333241789
9	78.41871311177513	8.762397485886066	8.5385808280723	4.2803085742665115
10	39.88146432993963	30.345657775943245	13.120819882681262	16.652058011435862
11	31.90020506934041	28.2278687095761	23.28795057286211	16.58397564822139
12	31.200305219483397	25.282730925015663	25.599133018795005	17.91783083670593
13	22.91448427609865	32.0087421701171	25.36331149983473	19.713462053949517
14	17.64254128109958	32.45160643131657	30.273793059216857	19.632059228366995
15	17.345873205643272	27.900777355871774	33.32072548829362	21.43262395019134
16	17.78725741546851	26.952228875144513	33.565427315499264	21.695086393887717
17	18.401149835467624	25.629885197348408	28.889945024313956	27.07901994287002
18	21.546916806312254	26.074065059769406	27.698010317602577	24.68100781631576
19	23.437271311506414	30.17397181653283	26.576789176548747	19.811967695412005
20	26.981336552170987	28.427511194944145	24.596315987679397	19.994836265205475
21	21.628484082047468	30.399926326331595	25.355253442352822	22.616336149268115
22	20.798504161411113	27.751949967685547	26.539787892193058	24.909757978710285
23	22.237442997465823	29.276238432987384	24.42545227903244	24.06086629051435
24	19.057799295166646	29.045021518302477	28.981543759363383	22.915635427167494
25	22.745100618825926	26.944170817662606	27.00041276988326	23.310315793628213
26	24.14013126411188	31.217572485516055	24.113161439070396	20.52913481130167
27	19.915571291607943	30.071683821558427	26.279956650939752	23.732788235893874
28	22.43626323207041	28.09499298620099	29.197137909542548	20.27160587218605
29	22.10933632851878	28.19234747659463	28.543448552591983	21.154867642294604
30	22.91941778067941	28.76233170582499	26.771498157336037	21.546752356159562
31	28.438858255479886	28.50677616854167	22.30980106465029	20.744564511328146
32	25.71539927674823	27.50412358757875	24.282709546495816	22.497767589177204
33	26.163197042528452	26.536005538681138	25.012539324142764	22.28825809464764
34	23.176288919184262	27.73961620623365	25.47563095412334	23.60846392045875
35	23.771105121471106	25.150019651793247	27.58256631041282	23.49630891632283
36	26.143956374663496	27.752936668601695	26.70654034702271	19.396566609712096
37	22.160973676464057	29.833888900765842	25.559500531996243	22.445636890773855
38	24.43531928819396	27.271097721214232	26.929205853767634	21.36437713682417
39	22.34170439427253	25.64123225788415	28.8869849215655	23.13007842627782
40	24.672291958223084	25.57364324512775	25.36512045151434	24.388944345134824
41	20.279499479515266	24.678376613872686	28.317494042793218	26.724629863818826
42	26.22864820329986	24.82917740389122	24.606347446993603	24.33582694581532
43	23.004931860079232	23.983574718749125	27.074086438289257	25.937406982882383
44	23.08929478841021	25.514276740005954	26.694699936028893	24.701728535554945
45	25.040824750405783	26.28834360872704	26.271076342694382	22.399755298172792
46	21.710051357782685	26.461674069664376	29.58524026989559	22.24303430265735
47	22.027604602630873	26.7395948277138	26.409050020802944	24.823750548852384
48	23.410465936617623	22.831930299447283	31.389093994773777	22.36850976916132
49	22.73786481210748	21.837993576577038	30.475408946417204	24.94873266489828
50	21.825002014514368	23.84543659048787	26.953708926518736	27.375852468479017
51	20.260094361497615	25.114005068353705	30.190087931496645	24.435812638652035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1964.0
1	1619.0
2	1274.0
3	914.0
4	554.0
5	558.0
6	562.0
7	611.0
8	660.0
9	714.0
10	768.0
11	836.5
12	905.0
13	950.5
14	996.0
15	1067.5
16	1139.0
17	1252.5
18	1366.0
19	1386.0
20	1406.0
21	1484.5
22	1563.0
23	1811.5
24	2060.0
25	2763.5
26	4235.5
27	5004.0
28	6248.5
29	7493.0
30	8261.5
31	9030.0
32	10546.5
33	12063.0
34	12594.0
35	13125.0
36	14642.0
37	16159.0
38	17837.5
39	19516.0
40	22748.0
41	25980.0
42	31960.5
43	37941.0
44	45342.5
45	52744.0
46	56010.5
47	59277.0
48	61288.0
49	63299.0
50	60288.0
51	57277.0
52	52536.5
53	47796.0
54	42526.0
55	37256.0
56	32262.5
57	27269.0
58	25876.5
59	24484.0
60	23122.0
61	21760.0
62	18805.0
63	15850.0
64	14527.0
65	13204.0
66	10621.0
67	8038.0
68	6587.5
69	5137.0
70	4342.0
71	3547.0
72	3072.5
73	2598.0
74	2138.0
75	1243.5
76	809.0
77	716.0
78	623.0
79	438.0
80	253.0
81	173.5
82	94.0
83	68.5
84	43.0
85	34.5
86	26.0
87	19.0
88	12.0
89	10.0
90	8.0
91	8.5
92	9.0
93	5.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	608087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.694897558940063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97551464651164	21.5952389024077
2	9.737927247760258	5.393817951262631
3	3.5270338317711527	2.9304252197345377
4	1.6454712331412091	1.8228462895211426
5	0.9137484886371366	1.2653085393720902
6	0.5761133074299585	0.9573239418968905
7	0.3939566610194595	0.7637412558717205
8	0.2958209584171327	0.6554184911319966
9	0.24433981705602315	0.6090269582643045
>10	2.994154504689444	20.68530901754775
>50	1.2701630258904382	25.57936220630416
>100	0.41983477172788847	15.029039664258171
>500	0.0023686023792828686	0.3922772375075624
>1k	0.002960752974103585	1.4351246260153339
>5k	5.921505948207172E-4	0.8857396989039902
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	5401	0.8881952746893126	TruSeq Adapter, Index 27 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	2089	0.3435363689735186	No Hit
AATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	2041	0.3356427616443042	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGT	1921	0.31590874332126817	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1586	0.2608179421694593	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	1114	0.18319747009885098	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT	668	0.1098527019982338	No Hit
GCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCTA	613	0.10080794360017563	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	610	0.10031459314209974	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1945659091544467	0.0
2	0.0	0.0	0.0	1.4938651870538262	0.0
3	0.0	0.0	0.0	1.8734161394668856	0.0
4	0.0	0.0	0.0	3.8548760292523934	0.0
5	0.0	0.0	0.0	4.064549973934651	0.0
6	0.0	0.0	0.0	5.417974730589537	0.0
7	0.0	0.0	0.0	6.366523211316801	0.0
8	0.0	0.0	0.0	6.956241458870195	0.0
9	0.0	0.0	0.0	8.271184879795161	0.0
10	0.0	0.0	0.0	9.813562861893118	0.0
11	0.0	0.0	0.0	11.694872608689217	0.0
12	0.0	0.0	0.0	12.472392930616836	0.0
13	0.0	0.0	0.0	12.752287090498564	0.0
14	0.0	0.0	0.0	13.124766686345868	0.0
15	0.0	0.0	0.0	13.397260589356458	0.0
16	0.0	0.0	0.0	13.973493924389109	0.0
17	0.0	0.0	0.0	14.604653610420877	0.0
18	0.0	0.0	0.0	15.40174350051884	0.0
19	0.0	0.0	0.0	15.76632948903693	0.0
20	1.6445015269196678E-4	0.0	0.0	16.10509680358238	0.0
21	1.6445015269196678E-4	0.0	0.0	16.59071810448176	0.0
22	1.6445015269196678E-4	0.0	0.0	17.08669976500073	0.0
23	1.6445015269196678E-4	0.0	0.0	17.55308039803515	0.0
24	1.6445015269196678E-4	0.0	0.0	17.901879171894812	0.0
25	1.6445015269196678E-4	0.0	0.0	18.15907921070505	0.0
26	1.6445015269196678E-4	0.0	0.0	18.42269280547027	0.0
27	1.6445015269196678E-4	0.0	0.0	18.694035557412015	0.0
28	1.6445015269196678E-4	0.0	0.0	18.980836623706804	0.0
29	1.6445015269196678E-4	0.0	0.0	19.28128705267503	0.0
30	1.6445015269196678E-4	0.0	0.0	19.658371252797707	0.0
31	3.2890030538393355E-4	0.0	0.0	19.957670530697087	0.0
32	3.2890030538393355E-4	0.0	0.0	20.234933488135745	0.0
33	3.2890030538393355E-4	0.0	0.0	20.530285962370517	0.0
34	3.2890030538393355E-4	0.0	0.0	20.810015672099553	0.0
35	3.2890030538393355E-4	0.0	0.0	21.16703695359381	0.0
36	3.2890030538393355E-4	0.0	0.0	21.448082264544382	0.0
37	3.2890030538393355E-4	0.0	0.0	21.73817233389301	0.0
38	3.2890030538393355E-4	0.0	0.0	22.020368795912425	0.0
39	3.2890030538393355E-4	0.0	0.0	22.321805925796802	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	30	2.163808E-6	45.000004	1
CGAAACG	30	2.163808E-6	45.000004	1
TTTCGCG	30	2.163808E-6	45.000004	1
GAGTACG	60	0.0	45.000004	1
GGCGTAA	35	1.2106648E-7	45.000004	10
TAGCGCG	35	1.2106648E-7	45.000004	1
CGCTACG	35	1.2106648E-7	45.000004	1
CATCGCG	35	1.2106648E-7	45.000004	1
CGTAACG	30	2.163808E-6	45.000004	1
CTTTCGT	30	2.163808E-6	45.000004	14
CCGCGTC	30	2.163808E-6	45.000004	41
ACGTAAA	30	2.163808E-6	45.000004	25
CGCGTCA	30	2.163808E-6	45.000004	42
TGTCGCC	35	1.2106648E-7	45.000004	26
CGATAAG	30	2.163808E-6	45.000004	1
CCGTTGG	35	1.2106648E-7	45.000004	2
TGCGTAA	30	2.163808E-6	45.000004	12
ATTTCGG	30	2.163808E-6	45.000004	2
TCACGAC	20	7.0306635E-4	45.0	25
TCGCCCG	20	7.0306635E-4	45.0	2
>>END_MODULE