FastQCFastQC Report
Sat 18 Jun 2016
SRR3552918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627145
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT51270.8175142909534477TruSeq Adapter, Index 27 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC19040.30359805148729563No Hit
AATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT18930.3018440711478207No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGT17920.2857393425762782No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17020.27138859434421064No Hit
AAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT9720.15498808090632946No Hit
GCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCTA6450.10284702899648407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGA302.163917E-645.00000432
GGCGTAA302.163917E-645.00000410
TAGCGCG302.163917E-645.0000041
GTCTACG302.163917E-645.0000041
CGATTGC302.163917E-645.00000427
ACCCTTA302.163917E-645.00000441
CGTAGCG302.163917E-645.0000041
CGAATCG302.163917E-645.0000041
CGACTGA302.163917E-645.00000443
TGCAACG650.045.0000041
TCACGGA207.030833E-445.041
CGGGTTA406.8066583E-945.06
AGCCGTA207.030833E-445.026
TAATACG253.8887716E-545.01
TCGTTGC207.030833E-445.044
CTCACCG253.8887716E-545.01
AATCATC207.030833E-445.020
ATAGGTT207.030833E-445.036
CACGGTA207.030833E-445.043
CGAGTAA207.030833E-445.016