##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552918_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	627145
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219400617082172	31.0	31.0	34.0	28.0	34.0
2	31.439096221766896	31.0	31.0	34.0	28.0	34.0
3	31.42373613757584	31.0	31.0	34.0	28.0	34.0
4	35.44475998373582	37.0	35.0	37.0	33.0	37.0
5	35.142249400059	37.0	35.0	37.0	32.0	37.0
6	35.188575209879694	37.0	35.0	37.0	32.0	37.0
7	35.217520669063774	37.0	35.0	37.0	33.0	37.0
8	35.57522741949629	37.0	35.0	37.0	33.0	37.0
9	36.025845697565956	39.0	37.0	39.0	32.0	39.0
10	35.77157276227985	38.0	35.0	39.0	30.0	39.0
11	36.199020960065056	38.0	35.0	39.0	32.0	39.0
12	35.97483835476644	37.0	35.0	39.0	32.0	39.0
13	35.71995471541669	37.0	35.0	39.0	30.0	39.0
14	36.75614092434764	39.0	35.0	40.0	31.0	41.0
15	36.84710553380797	38.0	35.0	40.0	31.0	41.0
16	36.89515343341652	38.0	35.0	40.0	32.0	41.0
17	36.897076433679615	38.0	35.0	40.0	32.0	41.0
18	36.81668832566631	38.0	35.0	40.0	31.0	41.0
19	36.860659018249365	38.0	35.0	40.0	31.0	41.0
20	36.77501534732797	38.0	35.0	40.0	31.0	41.0
21	36.798000462413	38.0	35.0	40.0	31.0	41.0
22	36.79995535322772	38.0	35.0	40.0	31.0	41.0
23	36.73405512281849	38.0	35.0	40.0	31.0	41.0
24	36.35995981790495	38.0	35.0	40.0	30.0	41.0
25	36.535048513501664	38.0	35.0	40.0	31.0	41.0
26	36.3880617719985	38.0	35.0	40.0	31.0	41.0
27	36.23000422549809	38.0	35.0	40.0	30.0	41.0
28	36.256691833626995	38.0	35.0	40.0	30.0	41.0
29	35.993808449401655	38.0	35.0	40.0	30.0	41.0
30	35.96851605290642	38.0	35.0	40.0	30.0	41.0
31	35.91843353610409	38.0	34.0	40.0	30.0	41.0
32	35.780460659018246	38.0	34.0	40.0	30.0	41.0
33	35.79496607642571	38.0	34.0	40.0	30.0	41.0
34	35.73222460515511	38.0	34.0	40.0	29.0	41.0
35	35.64494335440767	38.0	34.0	40.0	29.0	41.0
36	35.46424829983496	38.0	34.0	40.0	28.0	41.0
37	35.30478119095265	38.0	34.0	40.0	27.0	41.0
38	35.30612218864856	38.0	34.0	40.0	27.0	41.0
39	35.202634159564376	38.0	34.0	40.0	27.0	41.0
40	35.10605202943498	37.0	34.0	40.0	27.0	41.0
41	35.145720686603575	37.0	34.0	40.0	27.0	41.0
42	35.159487837740876	37.0	34.0	40.0	27.0	41.0
43	35.08094300361161	37.0	34.0	40.0	27.0	41.0
44	34.98302784842421	37.0	34.0	40.0	27.0	41.0
45	34.75950856659943	37.0	34.0	40.0	26.0	41.0
46	34.53349225458227	37.0	33.0	40.0	26.0	41.0
47	34.42231381897329	36.0	33.0	40.0	26.0	41.0
48	34.37462628259812	36.0	33.0	40.0	25.0	41.0
49	34.236042701448625	36.0	33.0	40.0	24.0	41.0
50	34.13862184981145	36.0	33.0	39.0	24.0	41.0
51	32.293936808871955	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	12.0
11	11.0
12	11.0
13	16.0
14	28.0
15	37.0
16	89.0
17	215.0
18	418.0
19	782.0
20	1264.0
21	1976.0
22	2692.0
23	3552.0
24	4546.0
25	5375.0
26	6450.0
27	8091.0
28	10160.0
29	12719.0
30	16136.0
31	21139.0
32	27810.0
33	38596.0
34	60981.0
35	67084.0
36	63973.0
37	85799.0
38	109569.0
39	77609.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.13633210820464	32.99380526034649	20.974894163231788	25.894968468217076
2	23.614634574141547	30.454360634303075	22.41602819124764	23.514976600307744
3	21.719379090959826	30.787457446045174	21.152365083035026	26.340798379959978
4	19.866378588683638	31.777340168541567	20.55011201556259	27.80616922721221
5	17.647752912005995	34.19655741495188	19.18870436661378	28.966985306428338
6	20.062664933946696	36.81859856970876	20.928333957856633	22.19040253848791
7	83.1812419775331	6.251345382646756	5.623579873872869	4.943832765947269
8	85.89114160202186	4.578685949820217	4.610735954205168	4.919436493952754
9	78.02422087396057	9.750536159899227	8.081863046026038	4.143379920114168
10	40.16503360466878	30.4886429773019	12.983281378309641	16.363042039719684
11	32.11235041338127	28.354686715193456	22.844158846837654	16.688804024587615
12	31.45763738848273	25.378182079104512	25.28793181800062	17.87624871441214
13	23.034864345566017	32.017316569533364	25.342624114040614	19.60519497086001
14	17.668800676079695	32.670435066850565	30.06529590445591	19.59546835261383
15	17.42451905061828	27.82227395578375	33.55364389415526	21.199563099442713
16	17.918822600833938	26.99646812140733	33.64915609627758	21.435553181481158
17	18.567635873681525	25.449138556474182	28.807692001052388	27.175533568791906
18	21.720814165783032	26.156152086040706	27.79421027035215	24.32882347782411
19	23.76818758022467	29.992585446746766	26.354511317159506	19.884715655869055
20	27.299587814620224	28.298399891572124	24.410303837230625	19.99170845657703
21	21.881383093224056	30.30001036442928	25.34214575576621	22.476460786580457
22	21.01858421896053	27.665850800054216	26.366789179535832	24.948775801449425
23	22.127099793508677	29.408350540943484	24.53738768546349	23.92716198008435
24	19.126836696457758	28.87099474603162	29.265321416897212	22.736847140613413
25	22.729831219255516	26.95198080188792	27.119565650686845	23.198622328169723
26	24.083585135813887	31.293719953120892	24.204290873721387	20.418404037343834
27	19.990113928995687	30.119190936705227	26.2744660325762	23.616229101722887
28	22.144958502419694	28.353411093128383	29.34600451251306	20.155625891938868
29	21.88855846734009	28.39024468025736	28.55942405663762	21.161772795764936
30	22.873019796059925	28.82060767446125	26.826013122961996	21.480359406516833
31	28.215803362858672	28.706120594120975	22.44026501048402	20.637811032536334
32	25.83341970357732	27.64703537459439	24.26185331940779	22.257691602420493
33	26.005947587878403	26.883735021406537	24.78198821644117	22.328329174273893
34	23.135479035948624	27.784324199347836	25.490436820830908	23.58975994387263
35	23.605386314169767	25.348364413333442	27.730269714340384	23.315979558156407
36	26.036243611923876	27.923526457198893	26.57359940683574	19.466630524041488
37	22.18211099506494	29.996412312941985	25.69581197330761	22.125664718685474
38	24.352581938786084	27.479291073037338	26.677881510655432	21.490245477521146
39	22.295800811614537	25.906130161286466	28.781701201476533	23.016367825622464
40	24.74818423171675	25.501757966658428	25.463329851948114	24.28672794967671
41	20.262299787130566	24.96105366382575	28.218035701472544	26.558610847571135
42	26.148976711924675	25.100734279951208	24.53659042167282	24.213698586451297
43	22.964545679228888	24.289917004839392	26.90908800994985	25.83644930598187
44	22.98511508502818	25.741415462133954	26.756172814899266	24.517296637938596
45	24.87000613893119	26.69589967232458	26.171937909095984	22.262156279648245
46	21.840084828867326	26.70594519608703	29.192451506429933	22.26151846861571
47	22.142566711047685	27.04175270471741	26.376196892265746	24.439483691969162
48	23.484521123504134	22.936641446555424	31.092490572355675	22.486346857584767
49	22.68598171076864	22.167600794074733	30.531376316481833	24.615041178674787
50	21.866553986717584	24.197593857879756	26.934600451251306	27.00125170415135
51	20.357493083736617	25.626928381793686	29.613725693420186	24.401852841049518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2056.0
1	1769.0
2	1482.0
3	1042.0
4	602.0
5	602.0
6	602.0
7	608.0
8	614.0
9	672.5
10	731.0
11	811.5
12	892.0
13	951.0
14	1010.0
15	1054.0
16	1098.0
17	1194.5
18	1291.0
19	1367.5
20	1444.0
21	1566.5
22	1689.0
23	1917.5
24	2146.0
25	2940.0
26	4552.5
27	5371.0
28	6632.5
29	7894.0
30	8723.0
31	9552.0
32	11162.0
33	12772.0
34	13311.5
35	13851.0
36	15291.0
37	16731.0
38	18642.0
39	20553.0
40	24013.5
41	27474.0
42	33279.5
43	39085.0
44	46628.5
45	54172.0
46	57428.5
47	60685.0
48	62270.5
49	63856.0
50	61121.5
51	58387.0
52	53568.5
53	48750.0
54	43444.0
55	38138.0
56	33419.5
57	28701.0
58	26848.5
59	24996.0
60	23655.5
61	22315.0
62	19572.0
63	16829.0
64	15338.5
65	13848.0
66	11162.0
67	8476.0
68	6862.5
69	5249.0
70	4436.0
71	3623.0
72	3155.0
73	2687.0
74	2264.5
75	1345.0
76	848.0
77	743.5
78	639.0
79	445.0
80	251.0
81	169.0
82	87.0
83	69.0
84	51.0
85	36.5
86	22.0
87	14.0
88	6.0
89	6.5
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	627145.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.582364263138686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73255998245763	21.440477845424237
2	9.776945765670094	5.393425589793279
3	3.568532601319616	2.9528569828345055
4	1.749823277336066	1.930570521264101
5	0.9592570861403216	1.322928918595968
6	0.6237524508002565	1.03227403847989
7	0.43880937705844997	0.8472380056074997
8	0.2734902678958028	0.6034806553220333
9	0.2263045784931935	0.5617813786573792
>10	2.9372631399797378	19.94057610484407
>50	1.2417216179363564	24.796370162656775
>100	0.46630052319129	16.734623468383695
>500	0.0023285918761113108	0.4494359152562461
>1k	0.0023285918761113108	1.1707171340240408
>5k	5.821479690278277E-4	0.8232432788562964
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	5127	0.8175142909534477	TruSeq Adapter, Index 27 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	1904	0.30359805148729563	No Hit
AATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	1893	0.3018440711478207	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGT	1792	0.2857393425762782	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1702	0.27138859434421064	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	972	0.15498808090632946	No Hit
GCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCTA	645	0.10284702899648407	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1890551626816767E-4	0.0	0.0	1.0799735308421496	0.0
2	3.1890551626816767E-4	0.0	0.0	1.3387653572937679	0.0
3	3.1890551626816767E-4	0.0	0.0	1.677921373844964	0.0
4	3.1890551626816767E-4	0.0	0.0	3.445295745003149	0.0
5	3.1890551626816767E-4	0.0	0.0	3.6441333343963516	0.0
6	3.1890551626816767E-4	0.0	0.0	4.863309123089556	0.0
7	3.1890551626816767E-4	0.0	0.0	5.755766210366024	0.0
8	3.1890551626816767E-4	0.0	0.0	6.30731330075182	0.0
9	3.1890551626816767E-4	0.0	0.0	7.493801274027538	0.0
10	3.1890551626816767E-4	0.0	0.0	8.921860175876391	0.0
11	3.1890551626816767E-4	0.0	0.0	10.707252708703729	0.0
12	3.1890551626816767E-4	0.0	0.0	11.44328664025066	0.0
13	3.1890551626816767E-4	0.0	0.0	11.723764041808513	0.0
14	3.1890551626816767E-4	0.0	0.0	12.049526026676446	0.0
15	3.1890551626816767E-4	0.0	0.0	12.304331534174713	0.0
16	3.1890551626816767E-4	0.0	0.0	12.853805738704764	0.0
17	3.1890551626816767E-4	0.0	0.0	13.450318506884372	0.0
18	3.1890551626816767E-4	0.0	0.0	14.210748710425818	0.0
19	3.1890551626816767E-4	0.0	0.0	14.564414927967217	0.0
20	3.1890551626816767E-4	0.0	0.0	14.895757759369843	0.0
21	3.1890551626816767E-4	0.0	0.0	15.369172998269937	0.0
22	3.1890551626816767E-4	0.0	0.0	15.827918583421697	0.0
23	3.1890551626816767E-4	0.0	0.0	16.291288298559344	0.0
24	3.1890551626816767E-4	0.0	0.0	16.6148179448134	0.0
25	4.783582744022515E-4	0.0	0.0	16.862448078195634	0.0
26	4.783582744022515E-4	0.0	0.0	17.119007566033375	0.0
27	4.783582744022515E-4	0.0	0.0	17.3872071052149	0.0
28	4.783582744022515E-4	0.0	0.0	17.65604445542897	0.0
29	4.783582744022515E-4	0.0	0.0	17.946089022474865	0.0
30	4.783582744022515E-4	0.0	0.0	18.308365688955504	0.0
31	4.783582744022515E-4	0.0	0.0	18.601599311164083	0.0
32	4.783582744022515E-4	0.0	0.0	18.87043666137815	0.0
33	4.783582744022515E-4	0.0	0.0	19.159683964633377	0.0
34	4.783582744022515E-4	0.0	0.0	19.434739972414672	0.0
35	4.783582744022515E-4	0.0	0.0	19.77389598896587	0.0
36	4.783582744022515E-4	0.0	0.0	20.045284583310078	0.0
37	4.783582744022515E-4	0.0	0.0	20.299611732533943	0.0
38	4.783582744022515E-4	0.0	0.0	20.564941122069058	0.0
39	4.783582744022515E-4	0.0	0.0	20.866147382184344	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGA	30	2.163917E-6	45.000004	32
GGCGTAA	30	2.163917E-6	45.000004	10
TAGCGCG	30	2.163917E-6	45.000004	1
GTCTACG	30	2.163917E-6	45.000004	1
CGATTGC	30	2.163917E-6	45.000004	27
ACCCTTA	30	2.163917E-6	45.000004	41
CGTAGCG	30	2.163917E-6	45.000004	1
CGAATCG	30	2.163917E-6	45.000004	1
CGACTGA	30	2.163917E-6	45.000004	43
TGCAACG	65	0.0	45.000004	1
TCACGGA	20	7.030833E-4	45.0	41
CGGGTTA	40	6.8066583E-9	45.0	6
AGCCGTA	20	7.030833E-4	45.0	26
TAATACG	25	3.8887716E-5	45.0	1
TCGTTGC	20	7.030833E-4	45.0	44
CTCACCG	25	3.8887716E-5	45.0	1
AATCATC	20	7.030833E-4	45.0	20
ATAGGTT	20	7.030833E-4	45.0	36
CACGGTA	20	7.030833E-4	45.0	43
CGAGTAA	20	7.030833E-4	45.0	16
>>END_MODULE