FastQCFastQC Report
Sat 18 Jun 2016
SRR3552917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686147
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT37960.5532342194894098No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18310.26685243832589806No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC16860.24571994047922677No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT15190.22138113261443976No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT14850.2164259262228065No Hit
AAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT11500.1676025691287727No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT8390.12227700478177417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGT351.210974E-745.00000443
GGTAATC351.210974E-745.0000048
ACGCATG253.889155E-545.0000041
CATGCAA253.889155E-545.00000436
GACGTTG253.889155E-545.0000041
TGTATCG253.889155E-545.00000414
CAATTCG351.210974E-745.0000041
TACGGGT351.210974E-745.0000044
TATGCGG253.889155E-545.0000042
TTATCCG253.889155E-545.0000041
TCGTGGC253.889155E-545.00000418
ATATACG351.210974E-745.0000041
CTACGGT253.889155E-545.0000043
CGTAGCG253.889155E-545.0000041
CGTAGAA253.889155E-545.00000417
TACCTCC253.889155E-545.00000413
TGCCTAA253.889155E-545.00000435
CGGCTGA351.210974E-745.00000432
CCGGTTA253.889155E-545.00000420
CCGCGTA253.889155E-545.00000414