##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552917_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686147
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.547828672281597	31.0	31.0	34.0	30.0	34.0
2	31.7394610775825	31.0	31.0	34.0	30.0	34.0
3	31.916982803976406	33.0	31.0	34.0	30.0	34.0
4	35.74633861257136	37.0	35.0	37.0	35.0	37.0
5	35.35905134031046	37.0	35.0	37.0	33.0	37.0
6	35.37897855707305	37.0	35.0	37.0	33.0	37.0
7	35.36328658436166	37.0	35.0	37.0	33.0	37.0
8	35.68353282897105	37.0	35.0	37.0	35.0	37.0
9	36.514360625347045	39.0	37.0	39.0	33.0	39.0
10	36.20757796798645	38.0	35.0	39.0	31.0	39.0
11	36.45958664834212	38.0	35.0	39.0	32.0	39.0
12	36.11571135631286	37.0	35.0	39.0	32.0	39.0
13	35.8344421822146	37.0	35.0	39.0	31.0	39.0
14	36.91995155557046	39.0	35.0	40.0	31.0	41.0
15	36.9703634935371	39.0	35.0	40.0	32.0	41.0
16	37.02244417012681	38.0	35.0	40.0	32.0	41.0
17	37.00475262589504	38.0	35.0	40.0	32.0	41.0
18	36.881247021410864	38.0	35.0	40.0	32.0	41.0
19	36.935270430388826	39.0	35.0	40.0	32.0	41.0
20	36.869163604883504	38.0	35.0	40.0	31.0	41.0
21	36.86017719235091	39.0	35.0	40.0	31.0	41.0
22	36.82595857738939	39.0	35.0	40.0	31.0	41.0
23	36.7765158194964	38.0	35.0	40.0	31.0	41.0
24	36.41673722977729	38.0	35.0	40.0	31.0	41.0
25	36.48792022700675	38.0	35.0	40.0	31.0	41.0
26	36.38724354985156	38.0	35.0	40.0	31.0	41.0
27	36.25969507991728	38.0	35.0	40.0	30.0	41.0
28	36.2041384717852	38.0	35.0	40.0	30.0	41.0
29	36.03695855261336	38.0	35.0	40.0	30.0	41.0
30	36.02230863065786	38.0	35.0	40.0	30.0	41.0
31	35.86597624124276	38.0	34.0	40.0	30.0	41.0
32	35.801267075422615	38.0	34.0	40.0	30.0	41.0
33	35.73173970009342	38.0	34.0	40.0	29.0	41.0
34	35.586977717602785	38.0	34.0	40.0	29.0	41.0
35	35.54913014266622	38.0	34.0	40.0	29.0	41.0
36	35.31074390764661	38.0	34.0	40.0	27.0	41.0
37	35.16747431672805	38.0	34.0	40.0	27.0	41.0
38	35.07212740127115	37.0	34.0	40.0	27.0	41.0
39	35.04931596290591	37.0	34.0	40.0	27.0	41.0
40	34.953259287004094	37.0	34.0	40.0	26.0	41.0
41	34.97829765341829	37.0	34.0	40.0	27.0	41.0
42	34.928838863975216	37.0	34.0	40.0	27.0	41.0
43	34.80970695783848	37.0	34.0	40.0	26.0	41.0
44	34.621943985763984	37.0	33.0	40.0	26.0	41.0
45	34.41171206753072	36.0	33.0	40.0	25.0	41.0
46	34.31671347393488	36.0	33.0	40.0	24.0	41.0
47	34.252113614138075	36.0	33.0	40.0	24.0	41.0
48	34.19534006561276	36.0	33.0	40.0	24.0	41.0
49	34.04225187897054	36.0	33.0	39.0	24.0	41.0
50	33.84851205353955	35.0	33.0	39.0	24.0	41.0
51	31.952601993450383	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	11.0
11	7.0
12	10.0
13	19.0
14	34.0
15	70.0
16	138.0
17	267.0
18	574.0
19	1031.0
20	1591.0
21	2410.0
22	3396.0
23	4362.0
24	5279.0
25	6175.0
26	7076.0
27	8674.0
28	10672.0
29	13494.0
30	17069.0
31	21964.0
32	29456.0
33	40353.0
34	66888.0
35	74667.0
36	68234.0
37	91377.0
38	119335.0
39	91507.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.536484164472046	32.76193002374127	20.23225343840314	27.469332373383548
2	22.29813727962084	31.752233850763755	21.025960909251225	24.923667960364178
3	20.282242726412854	32.65349844858317	21.19924739159393	25.86501143341004
4	20.231233248851925	32.92370293829165	19.574376919231593	27.270686893624834
5	18.36690971468213	35.390958497231644	17.583404139346236	28.658727648739994
6	20.636685724779092	37.271896546949854	19.234653798675794	22.85676392959526
7	83.80055585756405	7.0894429327826245	4.610090840592468	4.499910369060857
8	86.2976883962183	5.476086028212614	3.9272925480982943	4.298933027470789
9	78.93760374963382	10.571932836549603	6.82040437398983	3.6700590398267425
10	43.78755572785423	32.434303436435634	10.771452764495072	13.006688071215061
11	35.84348543387933	25.640861214870863	21.432142092000696	17.08351125924911
12	33.85528173991871	25.438280718271738	23.43477418104284	17.271663360766716
13	25.353313502791675	32.57013438811217	22.030847617201562	20.045704491894593
14	18.996075185055098	34.25053232033369	26.215373673571406	20.538018821039806
15	17.979529167947977	28.98897758060591	32.42818229912832	20.603310952317795
16	19.663133410187612	25.79461835437596	33.48043495052809	21.061813284908336
17	20.257612435819148	26.32890619648559	26.390263310923167	27.02321805677209
18	22.434113972661834	27.42211217129857	25.875359070286684	24.268414785752906
19	24.754753718955268	30.102004380985413	24.148761125531408	20.994480774527908
20	29.31077451333315	28.065560295388597	22.938524835057212	19.68514035622104
21	23.464359678028178	29.03124257629925	24.245679132897177	23.258718612775397
22	22.93925354187951	27.247076792582348	24.374368757715185	25.43930090782296
23	23.204065601102972	29.259182070314377	22.558431356546045	24.978320972036606
24	20.54880368200983	28.916689863833845	27.671329904524832	22.863176549631493
25	20.66889456632471	28.008866904613733	27.11969884004448	24.202539689017076
26	24.54736375732897	31.47415932737446	22.61279288548955	21.365684029807024
27	21.261478954218266	30.823278393696977	25.38989458527109	22.52534806681367
28	20.37129070009779	31.20759837177748	27.100315238571326	21.320795689553403
29	21.86849173719334	28.95618577360245	27.183825040406795	21.991497448797414
30	22.911708423996608	28.236660657264405	25.02699858776618	23.824632330972808
31	27.5191759200288	29.309317099688553	20.369104579630896	22.802402400651754
32	27.56216962254444	27.581115999924215	21.714005890865952	23.142708486665395
33	27.418322895822616	28.88040026408335	21.308116190845404	22.39316064924863
34	22.095702524386173	29.70413045601015	23.275770352417194	24.92439666718648
35	24.069769305994196	27.7236510543659	24.78914868096778	23.41743095867212
36	27.609681307358336	27.138499476059796	24.652151798375566	20.5996674182063
37	24.37728358500438	29.835151942659515	23.026698360555393	22.76086611178071
38	24.528271638584734	30.00858416636668	22.81333300298624	22.64981119206234
39	23.46275652301912	28.194249920206605	25.257269943612666	23.08572361316161
40	26.343480332931573	26.313749094581773	22.317229398365075	25.025541174121578
41	21.529788806188762	23.52877736111941	26.976580820144953	27.964853012546875
42	25.97038243991448	24.93751338998786	23.19007443011483	25.90202973998283
43	25.28875007833598	24.32292205606087	25.441195545560934	24.947132320042208
44	23.978680953206823	26.812184561034297	23.969062023152475	25.24007246260641
45	24.896122842481276	28.568222261410455	22.90966804489417	23.625986851214098
46	21.80290812318643	27.85875329922014	27.15540547433713	23.182933103256296
47	23.696671412977103	26.8560527117367	24.4058488924385	25.041426982847696
48	24.646467885161634	23.25565804412174	28.440261343414747	23.657612727301874
49	23.28466057564924	24.30368419595218	27.94561515243818	24.4660400759604
50	21.998055810198107	26.79454985593466	25.30478162842656	25.90261270544067
51	21.10320383241492	27.21894870924161	26.076919377334594	25.600928081008878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2186.0
1	1893.5
2	1601.0
3	1221.0
4	841.0
5	798.0
6	755.0
7	798.0
8	841.0
9	882.0
10	923.0
11	1100.0
12	1277.0
13	1350.5
14	1424.0
15	1522.5
16	1621.0
17	1697.0
18	1773.0
19	1753.5
20	1734.0
21	1883.0
22	2032.0
23	2659.5
24	3287.0
25	3043.5
26	3704.5
27	4609.0
28	6124.0
29	7639.0
30	8733.5
31	9828.0
32	10850.0
33	11872.0
34	13763.5
35	15655.0
36	15680.5
37	15706.0
38	18799.0
39	21892.0
40	24658.0
41	27424.0
42	34386.0
43	41348.0
44	45234.0
45	49120.0
46	52168.0
47	55216.0
48	54341.5
49	53467.0
50	57663.5
51	61860.0
52	57359.5
53	52859.0
54	49512.5
55	46166.0
56	41404.5
57	36643.0
58	34798.5
59	32954.0
60	33220.5
61	33487.0
62	28838.5
63	24190.0
64	21426.0
65	18662.0
66	15924.0
67	13186.0
68	11692.0
69	10198.0
70	8723.0
71	7248.0
72	5838.0
73	4428.0
74	3579.0
75	2402.0
76	2074.0
77	1615.0
78	1156.0
79	974.0
80	792.0
81	573.5
82	355.0
83	238.0
84	121.0
85	103.5
86	86.0
87	63.0
88	40.0
89	31.5
90	23.0
91	16.0
92	9.0
93	6.0
94	3.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	686147.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.001320219010225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7911994035785	19.698840067287538
2	9.579885955858792	4.7901959288804905
3	3.3449044587458787	2.5088108242530236
4	1.5575062181236317	1.55758846809634
5	0.8761406036559914	1.0952335894440182
6	0.5240804131416577	0.7861621337679455
7	0.35205620719733755	0.6161308979861594
8	0.23812258563521482	0.47627032118757523
9	0.19865848184471932	0.4470051886940031
>10	2.3490914972472177	14.999897467856005
>50	1.411991479359509	26.141250680097635
>100	0.7711287897984798	24.919069134251778
>500	0.0017446352710372847	0.2963128706202528
>1k	0.0034892705420745693	1.6672324275772514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	3796	0.5532342194894098	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1831	0.26685243832589806	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC	1686	0.24571994047922677	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT	1519	0.22138113261443976	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1485	0.2164259262228065	No Hit
AAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1150	0.1676025691287727	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT	839	0.12227700478177417	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4574136445980236E-4	0.0	0.0	0.7735951625526308	0.0
2	7.287068222990117E-4	0.0	0.0	0.95125388582913	0.0
3	7.287068222990117E-4	0.0	0.0	1.199014205410794	0.0
4	7.287068222990117E-4	0.0	0.0	2.519722450145523	0.0
5	7.287068222990117E-4	0.0	0.0	2.6903855879279512	0.0
6	7.287068222990117E-4	0.0	0.0	3.691191537673414	0.0
7	7.287068222990117E-4	0.0	0.0	4.464786700226045	0.0
8	7.287068222990117E-4	0.0	0.0	4.850126867857762	0.0
9	7.287068222990117E-4	0.0	0.0	5.748330897023524	0.0
10	7.287068222990117E-4	0.0	0.0	6.69492105918994	0.0
11	7.287068222990117E-4	0.0	0.0	8.073197142886292	0.0
12	7.287068222990117E-4	0.0	0.0	8.620747449161769	0.0
13	7.287068222990117E-4	0.0	0.0	8.820413118471699	0.0
14	7.287068222990117E-4	0.0	0.0	9.068319179417822	0.0
15	7.287068222990117E-4	0.0	0.0	9.277749520146557	0.0
16	7.287068222990117E-4	0.0	0.0	9.690780547025637	0.0
17	7.287068222990117E-4	0.0	0.0	10.172455756565284	0.0
18	7.287068222990117E-4	0.0	0.0	10.753818059395435	0.0
19	7.287068222990117E-4	0.0	0.0	11.048361356968696	0.0
20	7.287068222990117E-4	0.0	0.0	11.299036503839556	0.0
21	7.287068222990117E-4	0.0	0.0	11.636864986657377	0.0
22	7.287068222990117E-4	0.0	0.0	11.977899779493315	0.0
23	7.287068222990117E-4	0.0	0.0	12.316748451862356	0.0
24	7.287068222990117E-4	0.0	0.0	12.56450877144402	0.0
25	7.287068222990117E-4	0.0	0.0	12.769129647145583	0.0
26	7.287068222990117E-4	0.0	0.0	12.96311140324158	0.0
27	7.287068222990117E-4	0.0	0.0	13.157530383430956	0.0
28	7.287068222990117E-4	0.0	0.0	13.364191638234955	0.0
29	7.287068222990117E-4	0.0	0.0	13.598980976379696	0.0
30	7.287068222990117E-4	0.0	0.0	13.870059914274929	0.0
31	7.287068222990117E-4	0.0	0.0	14.107618338344407	0.0
32	7.287068222990117E-4	0.0	0.0	14.324918712753972	0.0
33	7.287068222990117E-4	0.0	0.0	14.535514984398388	0.0
34	7.287068222990117E-4	0.0	0.0	14.725270240925049	0.0
35	7.287068222990117E-4	0.0	0.0	14.981483559645381	0.0
36	7.287068222990117E-4	0.0	0.0	15.205196554091179	0.0
37	7.287068222990117E-4	0.0	0.0	15.424245824874262	0.0
38	7.287068222990117E-4	0.0	0.0	15.641254716554908	0.0
39	8.74448186758814E-4	0.0	0.0	15.877064244250867	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATAGT	35	1.210974E-7	45.000004	43
GGTAATC	35	1.210974E-7	45.000004	8
ACGCATG	25	3.889155E-5	45.000004	1
CATGCAA	25	3.889155E-5	45.000004	36
GACGTTG	25	3.889155E-5	45.000004	1
TGTATCG	25	3.889155E-5	45.000004	14
CAATTCG	35	1.210974E-7	45.000004	1
TACGGGT	35	1.210974E-7	45.000004	4
TATGCGG	25	3.889155E-5	45.000004	2
TTATCCG	25	3.889155E-5	45.000004	1
TCGTGGC	25	3.889155E-5	45.000004	18
ATATACG	35	1.210974E-7	45.000004	1
CTACGGT	25	3.889155E-5	45.000004	3
CGTAGCG	25	3.889155E-5	45.000004	1
CGTAGAA	25	3.889155E-5	45.000004	17
TACCTCC	25	3.889155E-5	45.000004	13
TGCCTAA	25	3.889155E-5	45.000004	35
CGGCTGA	35	1.210974E-7	45.000004	32
CCGGTTA	25	3.889155E-5	45.000004	20
CCGCGTA	25	3.889155E-5	45.000004	14
>>END_MODULE