##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704942
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.328533411259365	31.0	31.0	34.0	30.0	34.0
2	31.552777391615198	31.0	31.0	34.0	30.0	34.0
3	31.513202504603214	31.0	31.0	34.0	30.0	34.0
4	35.530185462066385	37.0	35.0	37.0	33.0	37.0
5	35.2117621024141	37.0	35.0	37.0	33.0	37.0
6	35.28282468628625	37.0	35.0	37.0	32.0	37.0
7	35.24016443906024	37.0	35.0	37.0	33.0	37.0
8	35.60933239897751	37.0	35.0	37.0	35.0	37.0
9	36.018956169443726	39.0	37.0	39.0	32.0	39.0
10	35.86511514422463	38.0	35.0	39.0	30.0	39.0
11	36.27927262100996	38.0	35.0	39.0	32.0	39.0
12	36.00764601910512	37.0	35.0	39.0	32.0	39.0
13	35.71796544963983	37.0	35.0	39.0	30.0	39.0
14	36.78925074686995	39.0	35.0	40.0	31.0	41.0
15	36.88263006034539	38.0	35.0	40.0	32.0	41.0
16	36.93582450754814	38.0	35.0	40.0	32.0	41.0
17	36.919258605672525	38.0	35.0	40.0	32.0	41.0
18	36.81587846943437	38.0	35.0	40.0	31.0	41.0
19	36.86266671584329	38.0	35.0	40.0	31.0	41.0
20	36.77989111160918	38.0	35.0	40.0	31.0	41.0
21	36.74694372019258	38.0	35.0	40.0	31.0	41.0
22	36.73581514507576	38.0	35.0	40.0	31.0	41.0
23	36.70093709837121	38.0	35.0	40.0	31.0	41.0
24	36.30365760587396	38.0	35.0	40.0	30.0	41.0
25	36.41739604109274	38.0	35.0	40.0	31.0	41.0
26	36.30477542833311	38.0	35.0	40.0	30.0	41.0
27	36.18600111782246	38.0	35.0	40.0	30.0	41.0
28	36.105927863568915	38.0	35.0	40.0	30.0	41.0
29	35.942957009229126	38.0	35.0	40.0	30.0	41.0
30	35.96774911978574	38.0	35.0	40.0	30.0	41.0
31	35.870365221535955	38.0	34.0	40.0	30.0	41.0
32	35.79237157099449	38.0	34.0	40.0	30.0	41.0
33	35.66568597132814	38.0	34.0	40.0	29.0	41.0
34	35.60810251056115	38.0	34.0	40.0	29.0	41.0
35	35.51723546050597	38.0	34.0	40.0	29.0	41.0
36	35.34159831588982	38.0	34.0	40.0	28.0	41.0
37	35.1732440399352	37.0	34.0	40.0	27.0	41.0
38	35.09161604784507	37.0	34.0	40.0	27.0	41.0
39	35.03268779559169	37.0	34.0	40.0	27.0	41.0
40	34.88088665450491	37.0	34.0	40.0	26.0	41.0
41	34.911534565964295	37.0	34.0	40.0	26.0	41.0
42	34.91992390863362	37.0	34.0	40.0	27.0	41.0
43	34.82454584916206	37.0	33.0	40.0	26.0	41.0
44	34.74208090878398	37.0	34.0	40.0	26.0	41.0
45	34.54730743805873	36.0	33.0	40.0	26.0	41.0
46	34.35426176905334	36.0	33.0	40.0	25.0	41.0
47	34.27372748396322	36.0	33.0	40.0	25.0	41.0
48	34.16137355981059	36.0	33.0	40.0	24.0	41.0
49	34.02333667166944	36.0	33.0	39.0	24.0	41.0
50	33.92696562270371	35.0	33.0	39.0	24.0	41.0
51	32.14927611065875	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	13.0
11	9.0
12	15.0
13	17.0
14	41.0
15	59.0
16	133.0
17	274.0
18	600.0
19	1020.0
20	1752.0
21	2440.0
22	3446.0
23	4426.0
24	5436.0
25	6250.0
26	7343.0
27	9063.0
28	11432.0
29	14131.0
30	18118.0
31	23526.0
32	31066.0
33	43119.0
34	70630.0
35	76662.0
36	71824.0
37	96098.0
38	119685.0
39	86305.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.671405590814565	32.54054943527269	20.443809561637696	27.344235412275054
2	22.34495887604938	31.608983434098125	21.01392738693396	25.03213030291854
3	20.63176828732009	32.61857003838614	20.98144244491036	25.76821922938341
4	20.253439290040884	32.8747613278823	19.553523552292244	27.31827582978458
5	18.44151717446258	35.352553827123366	17.56754456394994	28.638384434464108
6	20.739294863974624	37.222636755931696	19.124835802094356	22.913232577999324
7	84.11429592789193	7.058027468926522	4.399085314820226	4.428591288361312
8	86.65819315631641	5.447256653738889	3.7963406918583376	4.0982094980863675
9	78.40758530489033	11.543786581023687	6.462233772423831	3.586394341662151
10	44.0195079878912	32.478274808423954	10.71506592031685	12.787151283367992
11	35.96466092245887	25.83673550448122	21.170677871370977	17.027925701688932
12	34.20352312672532	25.397408581131497	23.23694715309912	17.16212113904406
13	25.364781783465872	32.811068144613316	21.896411335968065	19.927738735952747
14	18.999860981470814	34.38921216213532	26.03235443483294	20.578572421560924
15	18.136527544110013	28.886773663648924	32.57459478935856	20.402104002882506
16	19.797940823500372	25.689347492417813	33.686459311546194	20.826252372535613
17	20.430475131287395	26.20002780370584	26.293936238726022	27.075560826280743
18	22.51844832624528	27.499851051575874	25.934615897478093	24.047084724700756
19	24.947158773345894	29.898488102567306	24.169080576841782	20.98527254724502
20	29.59718671890737	27.930524780762106	22.86996660718187	19.602321893148655
21	23.541511216525617	28.964936122404396	24.35164878812725	23.141903872942738
22	23.14034346088047	27.15939751071719	24.31717786711531	25.38308116128703
23	23.093672954654426	29.234603697892876	22.7071163301378	24.9646070173149
24	20.719576929733226	28.82123635703363	27.824416760527814	22.63476995270533
25	20.77007753829393	27.973932607221585	27.22110471499783	24.034885139486654
26	24.34881167528676	31.65466095083	22.560721307568564	21.435806066314676
27	21.27026053207214	31.03801447494971	25.514581341443694	22.177143651534454
28	20.551194282650204	31.137313424366823	27.16209276791566	21.14939952506731
29	21.792431150364145	28.970610348085373	27.38665024924036	21.850308252310118
30	22.89592618967234	28.372972528236367	25.040357930155956	23.690743351935335
31	27.55673516402768	29.228220194001775	20.608787673312133	22.606256968658414
32	27.725543378036775	27.478572705272207	22.005640180326893	22.790243736364125
33	27.33004984807261	29.023664358202517	21.362324843746013	22.283960949978866
34	22.09032799861549	29.796493895951727	23.31312363286625	24.800054472566536
35	23.79983034065214	27.685540086985878	25.141642858561415	23.372986713800568
36	27.680291428230973	27.128898547681935	24.72813366206014	20.462676362026947
37	24.476056186182692	29.921185005291218	23.23694715309912	22.36581165542697
38	24.379452493964042	30.15907691696622	22.837339809516244	22.624130779553493
39	23.375114548430933	28.36006366481214	25.326197048835226	22.9386247379217
40	26.394795600205406	26.40415807257902	22.415602985777554	24.785443341438018
41	21.374382573318087	23.81032765816195	26.99158228620227	27.823707482317694
42	25.73062748424693	25.069438336770965	23.271559929753085	25.928374249229012
43	25.301656022764995	24.405412076454517	25.374569822765565	24.91836207801493
44	23.737555713803403	27.061942684646397	24.17390366867061	25.026597932879586
45	24.57932709357649	28.904363763259955	22.999622663992213	23.516686479171337
46	21.805765580714443	28.248423274538897	26.899659830170425	23.046151314576235
47	23.60222543131208	27.065914642623078	24.3844174414349	24.947442484629942
48	24.51832916750598	23.499521946486375	28.29055440022016	23.691594485787483
49	23.310286520025763	24.506838860501997	27.92853880177376	24.25433581769848
50	21.925633598225104	27.12322432200096	25.36577477296004	25.585367306813893
51	21.05946304802381	27.732494304495976	25.832763546504534	25.375279100975686
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2399.0
1	2051.5
2	1704.0
3	1246.5
4	789.0
5	775.5
6	762.0
7	798.5
8	835.0
9	924.0
10	1013.0
11	1114.0
12	1215.0
13	1285.0
14	1355.0
15	1504.5
16	1654.0
17	1684.5
18	1715.0
19	1695.0
20	1675.0
21	1886.0
22	2097.0
23	2786.0
24	3475.0
25	3249.5
26	3985.5
27	4947.0
28	6569.5
29	8192.0
30	9309.5
31	10427.0
32	11500.5
33	12574.0
34	14446.0
35	16318.0
36	16560.0
37	16802.0
38	19897.5
39	22993.0
40	25897.5
41	28802.0
42	35614.0
43	42426.0
44	46754.5
45	51083.0
46	53666.5
47	56250.0
48	55515.0
49	54780.0
50	58588.0
51	62396.0
52	57970.0
53	53544.0
54	50148.0
55	46752.0
56	42170.5
57	37589.0
58	35844.5
59	34100.0
60	33993.5
61	33887.0
62	29339.0
63	24791.0
64	21868.5
65	18946.0
66	16176.5
67	13407.0
68	11978.5
69	10550.0
70	9032.0
71	7514.0
72	6040.0
73	4566.0
74	3683.5
75	2501.5
76	2202.0
77	1698.0
78	1194.0
79	988.0
80	782.0
81	571.5
82	361.0
83	237.5
84	114.0
85	95.5
86	77.0
87	56.5
88	36.0
89	23.0
90	10.0
91	9.5
92	9.0
93	6.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	704942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.793008579306502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21080500324372	19.390811594398897
2	9.69412807755836	4.806932011916009
3	3.5092049482961802	2.610112451689561
4	1.7508103282087857	1.7363142195207542
5	0.9290331811829082	1.1516763815764128
6	0.5786746160738775	0.8608250832567925
7	0.3861711187073106	0.6702040701433519
8	0.2525508357352222	0.5009196029675513
9	0.1971247037769344	0.4398583024741308
>10	2.310212977213684	14.8187707945295
>50	1.3666919580791355	25.09620097099753
>100	0.8094000916779347	26.078834425560743
>500	0.0017307200819913072	0.2852068571177665
>1k	0.0034614401639826145	1.553333233851025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	3548	0.5033038179027495	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1976	0.2803067486403137	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC	1559	0.22115294591611795	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT	1465	0.20781851556581962	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1255	0.17802883074068504	No Hit
AAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1057	0.1499414136198439	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT	768	0.10894513307477778	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6844534727679724	0.0
2	5.674225680978009E-4	0.0	0.0	0.8382249887224764	0.0
3	5.674225680978009E-4	0.0	0.0	1.0624987587631323	0.0
4	5.674225680978009E-4	0.0	0.0	2.239475020639996	0.0
5	5.674225680978009E-4	0.0	0.0	2.3899838568279375	0.0
6	5.674225680978009E-4	0.0	0.0	3.290483472399148	0.0
7	5.674225680978009E-4	0.0	0.0	3.991959622210054	0.0
8	5.674225680978009E-4	0.0	0.0	4.3338317194889795	0.0
9	5.674225680978009E-4	0.0	0.0	5.1747519654099206	0.0
10	5.674225680978009E-4	0.0	0.0	6.045036329229922	0.0
11	5.674225680978009E-4	0.0	0.0	7.324432364648439	0.0
12	5.674225680978009E-4	0.0	0.0	7.826459481772969	0.0
13	5.674225680978009E-4	0.0	0.0	8.014985630023462	0.0
14	5.674225680978009E-4	0.0	0.0	8.24535919267117	0.0
15	5.674225680978009E-4	0.0	0.0	8.445091936641596	0.0
16	5.674225680978009E-4	0.0	0.0	8.808667947150262	0.0
17	5.674225680978009E-4	0.0	0.0	9.269982495013775	0.0
18	5.674225680978009E-4	0.0	0.0	9.830454136652378	0.0
19	5.674225680978009E-4	0.0	0.0	10.100972845993004	0.0
20	5.674225680978009E-4	0.0	0.0	10.339715891520154	0.0
21	5.674225680978009E-4	0.0	0.0	10.643996243662599	0.0
22	7.092782101222512E-4	0.0	0.0	10.957071645610561	0.0
23	7.092782101222512E-4	0.0	0.0	11.260642719542885	0.0
24	7.092782101222512E-4	0.0	0.0	11.492292982968811	0.0
25	7.092782101222512E-4	0.0	0.0	11.692876860791385	0.0
26	7.092782101222512E-4	0.0	0.0	11.887644657290954	0.0
27	8.511338521467014E-4	0.0	0.0	12.072624414490837	0.0
28	8.511338521467014E-4	0.0	0.0	12.268527056126603	0.0
29	8.511338521467014E-4	0.0	0.0	12.482587219941498	0.0
30	8.511338521467014E-4	0.0	0.0	12.741190055352071	0.0
31	8.511338521467014E-4	0.0	0.0	12.966740526170947	0.0
32	8.511338521467014E-4	0.0	0.0	13.173424196600571	0.0
33	8.511338521467014E-4	0.0	0.0	13.375994053411487	0.0
34	8.511338521467014E-4	0.0	0.0	13.562676078315663	0.0
35	8.511338521467014E-4	0.0	0.0	13.806809638239741	0.0
36	8.511338521467014E-4	0.0	0.0	14.013493308669366	0.0
37	8.511338521467014E-4	0.0	0.0	14.229397595830578	0.0
38	9.929894941711516E-4	0.0	0.0	14.45508992229148	0.0
39	0.0011348451361956019	0.0	0.0	14.674966167429377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	20	7.031427E-4	45.000004	31
CAAACGA	20	7.031427E-4	45.000004	39
CGAACGC	30	2.1643027E-6	45.000004	34
CGAACAT	25	3.8892642E-5	45.000004	24
CTCGTCA	25	3.8892642E-5	45.000004	19
ACGTTCA	20	7.031427E-4	45.000004	31
GCGGTCA	25	3.8892642E-5	45.000004	12
GACACGT	25	3.8892642E-5	45.000004	9
CCGGCAT	25	3.8892642E-5	45.000004	11
ATAGCGT	20	7.031427E-4	45.000004	30
TAAACCG	65	0.0	45.000004	1
TTCGTTC	25	3.8892642E-5	45.000004	33
TAACGAT	30	2.1643027E-6	45.000004	37
TCGATCC	20	7.031427E-4	45.000004	27
TCGATAG	40	6.8084773E-9	45.000004	1
ACCTCTA	20	7.031427E-4	45.000004	14
CCGTCCG	30	2.1643027E-6	45.000004	1
CCGTCCC	25	3.8892642E-5	45.000004	38
CCGTCCA	20	7.031427E-4	45.000004	39
CGTTAGA	20	7.031427E-4	45.000004	27
>>END_MODULE