FastQCFastQC Report
Sat 18 Jun 2016
SRR3552915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695084
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCC92561.3316376150220692No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGT74291.0687916856092212No Hit
AATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT72381.0413129923865319No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT48510.6979012608548032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20000.2877350075674307No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT16350.2352233686863746No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT13960.20083903528206665No Hit
AAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT11470.1650160268399215No Hit
AACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG9910.1425726962496619No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTAT8590.12358218575021149No Hit
AAAAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT7480.10761289283021908No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA7180.10329686771670762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT302.1642572E-645.00000442
TCCGCAA302.1642572E-645.00000417
GTCGAAC302.1642572E-645.00000440
TGCTTCG351.2109922E-745.0000041
ATTGTCG302.1642572E-645.00000445
TTGTACT302.1642572E-645.00000441
GCGCATC302.1642572E-645.00000439
GACCCGA302.1642572E-645.0000049
GGCCCAC351.2109922E-745.0000049
CTACGAA302.1642572E-645.00000410
TCGCTAT302.1642572E-645.00000438
CTCGACC351.2109922E-745.00000429
TGAGGTC302.1642572E-645.00000417
TACTACG551.8189894E-1245.0000041
CACCGTC551.8189894E-1245.00000429
TAGTGCG302.1642572E-645.0000041
GATTCGT302.1642572E-645.0000047
TCGACTT302.1642572E-645.00000436
TCGACGG302.1642572E-645.0000042
TCGACGA302.1642572E-645.00000444