##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695084
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40451945376386	31.0	31.0	34.0	30.0	34.0
2	31.598576574917566	31.0	31.0	34.0	30.0	34.0
3	31.771847719124594	31.0	31.0	34.0	30.0	34.0
4	35.587435475424556	37.0	35.0	37.0	33.0	37.0
5	35.175737033221885	37.0	35.0	37.0	32.0	37.0
6	35.196335982413636	37.0	35.0	37.0	32.0	37.0
7	35.15332966950757	37.0	35.0	37.0	32.0	37.0
8	35.506543094072086	37.0	35.0	37.0	33.0	37.0
9	36.382487584234426	39.0	37.0	39.0	32.0	39.0
10	36.04081089479833	38.0	35.0	39.0	31.0	39.0
11	36.27724850521663	38.0	35.0	39.0	32.0	39.0
12	35.861022552669894	37.0	35.0	39.0	31.0	39.0
13	35.59607903505188	37.0	35.0	39.0	30.0	39.0
14	36.59529207980618	38.0	35.0	40.0	31.0	41.0
15	36.705822605613136	38.0	35.0	40.0	31.0	41.0
16	36.72153005967624	38.0	35.0	40.0	31.0	41.0
17	36.71565307214667	38.0	35.0	40.0	31.0	41.0
18	36.50768684072716	38.0	35.0	40.0	31.0	41.0
19	36.559854636274174	38.0	35.0	40.0	31.0	41.0
20	36.51107492044127	38.0	35.0	40.0	31.0	41.0
21	36.589497096753774	38.0	35.0	40.0	31.0	41.0
22	36.533945537517766	38.0	35.0	40.0	31.0	41.0
23	36.498696560415716	38.0	35.0	40.0	31.0	41.0
24	36.11034062070196	38.0	34.0	40.0	30.0	41.0
25	36.23931208314391	38.0	35.0	40.0	30.0	41.0
26	36.08342732676914	38.0	35.0	40.0	30.0	41.0
27	35.97237743927353	38.0	34.0	40.0	30.0	41.0
28	35.94683520265177	38.0	34.0	40.0	30.0	41.0
29	35.71502580983018	38.0	34.0	40.0	29.0	41.0
30	35.726950987218814	38.0	34.0	40.0	29.0	41.0
31	35.52645147924567	38.0	34.0	40.0	29.0	41.0
32	35.51845388471034	38.0	34.0	40.0	29.0	41.0
33	35.376491186676716	38.0	34.0	40.0	28.0	41.0
34	35.16086257200568	37.0	34.0	40.0	27.0	41.0
35	35.18129751224313	37.0	34.0	40.0	27.0	41.0
36	34.81642650384702	37.0	33.0	40.0	25.0	41.0
37	34.85254156332184	37.0	33.0	40.0	26.0	41.0
38	34.78440879087995	37.0	33.0	40.0	26.0	41.0
39	34.690894913420536	37.0	33.0	40.0	25.0	41.0
40	34.44630720891288	37.0	33.0	40.0	24.0	41.0
41	34.477322165378574	37.0	33.0	40.0	24.0	41.0
42	34.50157534916643	37.0	33.0	40.0	25.0	41.0
43	34.370664552773476	37.0	33.0	40.0	24.0	41.0
44	34.26295239136565	36.0	33.0	40.0	24.0	41.0
45	34.020918335050155	36.0	33.0	40.0	23.0	41.0
46	33.91527786569681	36.0	33.0	40.0	23.0	41.0
47	33.8000874714423	36.0	33.0	39.0	23.0	41.0
48	33.77310943713278	36.0	33.0	39.0	23.0	41.0
49	33.68709537264561	36.0	33.0	39.0	23.0	41.0
50	33.459554528661286	35.0	32.0	39.0	22.0	40.0
51	31.40114144477502	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	6.0
11	6.0
12	23.0
13	26.0
14	49.0
15	69.0
16	151.0
17	305.0
18	619.0
19	1173.0
20	1868.0
21	2646.0
22	3815.0
23	4891.0
24	6093.0
25	7048.0
26	8422.0
27	10179.0
28	12593.0
29	15945.0
30	20167.0
31	25397.0
32	33014.0
33	44611.0
34	72100.0
35	76144.0
36	66870.0
37	87444.0
38	110320.0
39	83081.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.4032433490053	36.513140857795605	20.36372582306599	24.719889970133107
2	20.741378020498242	34.78370959481156	20.77029538875877	23.704616995931428
3	20.181877298283375	30.50580936980279	24.878863561814114	24.43344977009973
4	23.271288074534876	30.721466757974575	18.854124105863466	27.153121061627083
5	16.95895747852058	37.34498276467305	18.63976152522573	27.056298231580644
6	20.8265475827382	35.99823906175369	22.154013040150545	21.021200315357568
7	78.74242537592579	10.091010582893578	6.110916090717093	5.055647950463541
8	81.26269630720891	5.304250997001801	4.817115629190141	8.615937066599146
9	76.14417825759189	9.890027680107728	8.562993825206737	5.402800237093646
10	43.991517571976914	29.862865495393365	12.471298432995148	13.674318499634577
11	34.646603863705685	23.5944144880331	24.208009391670647	17.55097225659057
12	32.776326314517384	22.31528851189209	28.06639197564611	16.841993197944422
13	25.173791944570727	32.732734460870915	22.368231753284494	19.72524184127386
14	17.893520783099596	32.85056194646978	31.057541246813336	18.19837602361729
15	16.06582801503128	27.192540757663824	34.01847834218598	22.72315288511892
16	18.008902521134136	25.911544503973623	33.81807090941527	22.26148206547698
17	18.498627504013903	25.738040294410457	27.196569047769763	28.56676315380587
18	21.236282233514224	26.467736273601467	27.718088748985735	24.57789274389858
19	23.526221291239622	29.51815895632758	27.163335654395727	19.792284098037072
20	27.174125717179503	29.615844991396724	24.217936249431723	18.992093041992046
21	21.45309056171628	29.042389121314834	25.167030171892897	24.33749014507599
22	21.15140040628183	28.238889112682784	25.70164181595318	24.908068665082205
23	22.457285738126615	27.29439895034269	22.519148764753613	27.72916654677708
24	19.64352509912471	31.344556916861848	26.705549257355944	22.306368726657496
25	20.525432897318886	25.713007348752093	26.684544601803523	27.0770151521255
26	21.810313573611246	32.86940858946545	21.662273912217803	23.658003924705504
27	18.41906877442151	30.043850815153277	26.921925983046652	24.61515442737856
28	19.405855982874012	28.343049185422192	27.10607638788981	25.14501844381398
29	20.74238509302473	29.484206225434622	27.486030465382598	22.287378216158046
30	24.567534283626152	27.31669841342917	24.812828377577386	23.302938925367293
31	26.00103009132709	29.429248838989245	19.847529219490017	24.722191850193646
32	29.79222655103556	25.866226240281748	21.07155394168187	23.269993267000824
33	26.21927709456699	25.93916706470009	24.076226758204765	23.765329082528154
34	20.806262264704696	31.2071634507484	22.207531751558086	25.779042532988818
35	25.93700905214334	25.667401350052653	24.769811993946057	23.62577760385795
36	25.989520691024392	27.6291786316474	25.489149512864635	20.892151164463577
37	26.634910312998137	28.727175420524713	22.359024233042337	22.27889003343481
38	25.35664754187983	25.904351128784437	24.79484493960442	23.944156389731315
39	25.921327494230912	26.39867987178528	25.673156050204003	22.0068365837798
40	26.228916217320496	26.46226930845768	23.922576264163755	23.386238210058067
41	21.215853047976935	24.956408146353535	28.12422671216716	25.70351209350237
42	27.926265026960774	24.01796041917236	22.72890758527027	25.3268669685966
43	23.856828814934598	23.459035166972626	26.48039661393443	26.203739404158345
44	22.80587669979456	24.255629535423058	25.312911820729582	27.625581944052808
45	25.0759620419978	28.137174787507696	23.476730869938024	23.31013230055648
46	21.53739691893354	25.647116032019152	29.997381611431134	22.818105437616172
47	22.367224680758007	27.029826610884438	25.10933930287562	25.493609405481926
48	22.847166673380485	22.508502569473617	30.885763447295577	23.75856730985032
49	23.429542328696964	21.940082062024157	29.147700134084513	25.482675475194366
50	22.32622244217965	24.022851914301004	25.356072071864695	28.29485357165465
51	19.903493678461885	24.882172514401137	29.42378187384546	25.790551933291518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2424.0
1	2029.5
2	1635.0
3	1218.5
4	802.0
5	743.5
6	685.0
7	794.5
8	904.0
9	964.0
10	1024.0
11	1087.5
12	1151.0
13	1242.5
14	1334.0
15	1429.5
16	1525.0
17	1685.0
18	1845.0
19	1837.5
20	1830.0
21	2017.0
22	2204.0
23	2701.0
24	3198.0
25	3131.0
26	3929.5
27	4795.0
28	4856.0
29	4917.0
30	7084.5
31	9252.0
32	10764.0
33	12276.0
34	12151.5
35	12027.0
36	14359.5
37	16692.0
38	19214.5
39	21737.0
40	25990.5
41	30244.0
42	34756.0
43	39268.0
44	44619.5
45	49971.0
46	60041.0
47	70111.0
48	69920.5
49	69730.0
50	67960.0
51	66190.0
52	60613.0
53	55036.0
54	50237.0
55	45438.0
56	40025.0
57	34612.0
58	31345.0
59	28078.0
60	27055.5
61	26033.0
62	23552.5
63	21072.0
64	18669.5
65	16267.0
66	13715.0
67	11163.0
68	9804.0
69	8445.0
70	6997.0
71	5549.0
72	4692.5
73	3836.0
74	3561.5
75	2832.0
76	2377.0
77	1909.0
78	1441.0
79	1054.5
80	668.0
81	489.5
82	311.0
83	229.5
84	148.0
85	174.0
86	200.0
87	190.5
88	181.0
89	121.5
90	62.0
91	41.5
92	21.0
93	17.0
94	13.0
95	9.5
96	6.0
97	5.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.71837378933237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.54431792575588	20.200321274427484
2	9.779684131331395	5.030351440623662
3	3.573289430561921	2.756975796979863
4	1.6874000537120626	1.735887412540254
5	0.892353798483308	1.1474944270862144
6	0.5608368362714298	0.8654286834033138
7	0.37499538903082014	0.6750990109059518
8	0.2754980739208624	0.5668289954670288
9	0.1958762639791938	0.45338570761273617
>10	2.2383571424016155	14.186433530159837
>50	1.1507669881941824	21.781659797362092
>100	0.7188108054287619	24.929125928719152
>500	0.0033484975408172133	0.6543525966919073
>1k	0.002790414617347678	1.5829907411526751
>5k	0.0016742487704086066	3.433664656867843
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCC	9256	1.3316376150220692	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGT	7429	1.0687916856092212	No Hit
AATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	7238	1.0413129923865319	No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	4851	0.6979012608548032	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2000	0.2877350075674307	No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT	1635	0.2352233686863746	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT	1396	0.20083903528206665	No Hit
AAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	1147	0.1650160268399215	No Hit
AACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG	991	0.1425726962496619	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTAT	859	0.12358218575021149	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT	748	0.10761289283021908	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	718	0.10329686771670762	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0226102168946487	0.0
2	2.877350075674307E-4	0.0	0.0	1.2368289300286008	0.0
3	2.877350075674307E-4	0.0	0.0	1.70727566740135	0.0
4	2.877350075674307E-4	0.0	0.0	4.279770502557964	0.0
5	2.877350075674307E-4	0.0	0.0	4.5374372018345985	0.0
6	2.877350075674307E-4	0.0	0.0	5.8050537776729145	0.0
7	2.877350075674307E-4	0.0	0.0	6.843633287487555	0.0
8	2.877350075674307E-4	0.0	0.0	7.372490231396493	0.0
9	2.877350075674307E-4	0.0	0.0	9.5156844352625	0.0
10	2.877350075674307E-4	0.0	0.0	10.756253920389478	0.0
11	2.877350075674307E-4	0.0	0.0	13.654896386623776	0.0
12	2.877350075674307E-4	0.0	0.0	14.378262195648295	0.0
13	2.877350075674307E-4	0.0	0.0	14.70095700663517	0.0
14	2.877350075674307E-4	0.0	0.0	15.140040628183069	0.0
15	2.877350075674307E-4	0.0	0.0	15.417417175478072	0.0
16	2.877350075674307E-4	0.0	0.0	15.894625685528656	0.0
17	2.877350075674307E-4	0.0	0.0	16.50002014145053	0.0
18	2.877350075674307E-4	0.0	0.0	17.27302023928043	0.0
19	2.877350075674307E-4	0.0	0.0	17.648658291659714	0.0
20	2.877350075674307E-4	0.0	0.0	17.987897865581715	0.0
21	2.877350075674307E-4	0.0	0.0	18.355047735237754	0.0
22	2.877350075674307E-4	0.0	0.0	18.73039805260947	0.0
23	2.877350075674307E-4	0.0	0.0	19.090786149587675	0.0
24	2.877350075674307E-4	0.0	0.0	19.351042463932416	0.0
25	2.877350075674307E-4	0.0	0.0	19.565117309562584	0.0
26	2.877350075674307E-4	0.0	0.0	19.780630830230592	0.0
27	2.877350075674307E-4	0.0	0.0	20.023191441609935	0.0
28	2.877350075674307E-4	0.0	0.0	20.236834684728752	0.0
29	2.877350075674307E-4	0.0	0.0	20.464145340707024	0.0
30	2.877350075674307E-4	0.0	0.0	20.777488763947954	0.0
31	2.877350075674307E-4	0.0	0.0	21.001058864827847	0.0
32	2.877350075674307E-4	0.0	0.0	21.223478025677473	0.0
33	2.877350075674307E-4	0.0	0.0	21.45956459938655	0.0
34	2.877350075674307E-4	0.0	0.0	21.676804530099957	0.0
35	2.877350075674307E-4	0.0	0.0	21.944541954641455	0.0
36	2.877350075674307E-4	0.0	0.0	22.16739271800243	0.0
37	4.3160251135114606E-4	0.0	0.0	22.414269354495286	0.0
38	4.3160251135114606E-4	0.0	0.0	22.65222620575355	0.0
39	4.3160251135114606E-4	0.0	0.0	22.922265510355583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	30	2.1642572E-6	45.000004	42
TCCGCAA	30	2.1642572E-6	45.000004	17
GTCGAAC	30	2.1642572E-6	45.000004	40
TGCTTCG	35	1.2109922E-7	45.000004	1
ATTGTCG	30	2.1642572E-6	45.000004	45
TTGTACT	30	2.1642572E-6	45.000004	41
GCGCATC	30	2.1642572E-6	45.000004	39
GACCCGA	30	2.1642572E-6	45.000004	9
GGCCCAC	35	1.2109922E-7	45.000004	9
CTACGAA	30	2.1642572E-6	45.000004	10
TCGCTAT	30	2.1642572E-6	45.000004	38
CTCGACC	35	1.2109922E-7	45.000004	29
TGAGGTC	30	2.1642572E-6	45.000004	17
TACTACG	55	1.8189894E-12	45.000004	1
CACCGTC	55	1.8189894E-12	45.000004	29
TAGTGCG	30	2.1642572E-6	45.000004	1
GATTCGT	30	2.1642572E-6	45.000004	7
TCGACTT	30	2.1642572E-6	45.000004	36
TCGACGG	30	2.1642572E-6	45.000004	2
TCGACGA	30	2.1642572E-6	45.000004	44
>>END_MODULE