FastQCFastQC Report
Sat 18 Jun 2016
SRR3552913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483659
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT47010.9719657858119046Illumina PCR Primer Index 8 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC37370.7726518063346283No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT36310.7507355388817327No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT32430.6705137297145303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22050.4558997144682514No Hit
AAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT13750.2842912051672769No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT10110.20903156976299417No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA9850.2036558815198311No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT7300.15093278528880885No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT6150.1271557026748184No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT6070.12550164475384518No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC6070.12550164475384518TruSeq Adapter, Index 14 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6040.12488137303348021No Hit
AACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG5590.11557729722800568TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGC351.2099736E-745.012
TAATACG207.02923E-445.01
AATCCCG207.02923E-445.043
GGTACGA351.2099736E-745.039
CTCACCG207.02923E-445.01
TCGCAAT253.8874437E-545.041
TCCGCGC207.02923E-445.029
ATTAGCG253.8874437E-545.042
CTACTAC207.02923E-445.021
TACTTGC207.02923E-445.014
CGAGTAG207.02923E-445.01
GAATGCG207.02923E-445.029
CGAAACG253.8874437E-545.02
CCAGGTG253.8874437E-545.04
ACGCACT253.8874437E-545.011
TAGACCC351.2099736E-745.021
TGGACGG502.1827873E-1145.02
GCGAAAC253.8874437E-545.01
TAGCGGA207.02923E-445.03
TAGCGAG207.02923E-445.01
Produced by FastQC (version 0.11.5)