FastQCFastQC Report
Sat 18 Jun 2016
SRR3552912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493065
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT44760.9077910620303611Illumina PCR Primer Index 8 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC35150.7128877531359963No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT34390.6974739638790017No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT31280.6343991157352479No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21770.44152393700627707No Hit
AAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT13020.26406254753430075No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT9570.19409205682820724No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA8830.17908389360429153No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT7320.1484591281068419No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6190.1255412572378895No Hit
AACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG5860.11884842769208928TruSeq Adapter, Index 14 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT5670.11499498037784064No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT5490.11134434608013141No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC5130.10404307748471298TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCT351.2100463E-745.00000424
TAGAGTA207.029362E-445.00000428
TCGCAGG800.045.0000042
CGAACGG406.8012014E-945.0000042
GTCGATC207.029362E-445.00000434
CCCTCCG207.029362E-445.00000437
TTGTGCG207.029362E-445.00000424
GTCGAAC207.029362E-445.00000418
TCTCTAA207.029362E-445.0000044
ACGTTCC207.029362E-445.00000417
CGTGCCG207.029362E-445.00000413
TTTCGCG207.029362E-445.0000041
ACTCTTG351.2100463E-745.00000419
ATTACCG207.029362E-445.0000041
CGATTCG207.029362E-445.00000427
TGCGGTA207.029362E-445.00000412
CTAAGCG207.029362E-445.0000041
CACGAGA351.2100463E-745.00000430
ACTTAGC207.029362E-445.00000430
TCGATGG207.029362E-445.0000042