Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552912_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 493065 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT | 4476 | 0.9077910620303611 | Illumina PCR Primer Index 8 (95% over 21bp) |
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC | 3515 | 0.7128877531359963 | No Hit |
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT | 3439 | 0.6974739638790017 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT | 3128 | 0.6343991157352479 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2177 | 0.44152393700627707 | No Hit |
AAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT | 1302 | 0.26406254753430075 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT | 957 | 0.19409205682820724 | No Hit |
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA | 883 | 0.17908389360429153 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT | 732 | 0.1484591281068419 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 619 | 0.1255412572378895 | No Hit |
AACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG | 586 | 0.11884842769208928 | TruSeq Adapter, Index 14 (95% over 21bp) |
AATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT | 567 | 0.11499498037784064 | No Hit |
AAAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT | 549 | 0.11134434608013141 | No Hit |
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC | 513 | 0.10404307748471298 | TruSeq Adapter, Index 14 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTCGCT | 35 | 1.2100463E-7 | 45.000004 | 24 |
TAGAGTA | 20 | 7.029362E-4 | 45.000004 | 28 |
TCGCAGG | 80 | 0.0 | 45.000004 | 2 |
CGAACGG | 40 | 6.8012014E-9 | 45.000004 | 2 |
GTCGATC | 20 | 7.029362E-4 | 45.000004 | 34 |
CCCTCCG | 20 | 7.029362E-4 | 45.000004 | 37 |
TTGTGCG | 20 | 7.029362E-4 | 45.000004 | 24 |
GTCGAAC | 20 | 7.029362E-4 | 45.000004 | 18 |
TCTCTAA | 20 | 7.029362E-4 | 45.000004 | 4 |
ACGTTCC | 20 | 7.029362E-4 | 45.000004 | 17 |
CGTGCCG | 20 | 7.029362E-4 | 45.000004 | 13 |
TTTCGCG | 20 | 7.029362E-4 | 45.000004 | 1 |
ACTCTTG | 35 | 1.2100463E-7 | 45.000004 | 19 |
ATTACCG | 20 | 7.029362E-4 | 45.000004 | 1 |
CGATTCG | 20 | 7.029362E-4 | 45.000004 | 27 |
TGCGGTA | 20 | 7.029362E-4 | 45.000004 | 12 |
CTAAGCG | 20 | 7.029362E-4 | 45.000004 | 1 |
CACGAGA | 35 | 1.2100463E-7 | 45.000004 | 30 |
ACTTAGC | 20 | 7.029362E-4 | 45.000004 | 30 |
TCGATGG | 20 | 7.029362E-4 | 45.000004 | 2 |