##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493065
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.139152038777848	31.0	31.0	34.0	28.0	34.0
2	31.331479622362163	31.0	31.0	34.0	28.0	34.0
3	31.32633831239289	31.0	31.0	34.0	28.0	34.0
4	35.35147495766278	37.0	35.0	37.0	33.0	37.0
5	34.99757841258252	37.0	35.0	37.0	32.0	37.0
6	35.05453844827761	36.0	35.0	37.0	32.0	37.0
7	34.96242686055591	36.0	35.0	37.0	32.0	37.0
8	35.34648778558608	37.0	35.0	37.0	33.0	37.0
9	35.835944550921276	39.0	37.0	39.0	32.0	39.0
10	35.69384563901311	38.0	35.0	39.0	30.0	39.0
11	36.050999361139	37.0	35.0	39.0	31.0	39.0
12	35.70018963017046	37.0	35.0	39.0	31.0	39.0
13	35.337647166195126	37.0	35.0	39.0	30.0	39.0
14	36.274935353350976	38.0	35.0	40.0	30.0	41.0
15	36.444651313721316	38.0	35.0	40.0	31.0	41.0
16	36.531518156835304	38.0	35.0	40.0	31.0	41.0
17	36.486402401306115	38.0	35.0	40.0	31.0	41.0
18	36.335661626763205	38.0	35.0	40.0	31.0	41.0
19	36.32336912983075	38.0	35.0	40.0	31.0	41.0
20	36.26848589942502	38.0	34.0	40.0	30.0	41.0
21	36.25324652936226	38.0	34.0	40.0	31.0	41.0
22	36.27416466388813	38.0	35.0	40.0	31.0	41.0
23	36.207307352985914	38.0	35.0	40.0	31.0	41.0
24	35.80608439049618	38.0	34.0	40.0	30.0	41.0
25	35.957405210266394	38.0	34.0	40.0	30.0	41.0
26	35.78088892945149	38.0	34.0	40.0	30.0	41.0
27	35.68273148570675	38.0	34.0	40.0	29.0	41.0
28	35.658998306511315	38.0	34.0	40.0	29.0	41.0
29	35.33929197975926	37.0	34.0	40.0	27.0	41.0
30	35.414977741271436	37.0	34.0	40.0	29.0	41.0
31	35.15635463884072	37.0	34.0	40.0	27.0	41.0
32	35.02392787969132	37.0	34.0	40.0	27.0	41.0
33	34.94859704095809	37.0	34.0	40.0	27.0	41.0
34	34.646866031861926	37.0	33.0	40.0	25.0	41.0
35	34.58079766359405	37.0	33.0	40.0	25.0	41.0
36	34.339584030503076	37.0	33.0	40.0	24.0	41.0
37	34.16566375629988	36.0	33.0	40.0	23.0	41.0
38	34.20372567511383	36.0	33.0	40.0	24.0	41.0
39	33.96674272154787	36.0	33.0	40.0	23.0	41.0
40	33.74685082088568	36.0	32.0	40.0	22.0	41.0
41	33.67680528936347	36.0	32.0	40.0	22.0	41.0
42	33.67472442781378	36.0	32.0	40.0	22.0	41.0
43	33.62460527516656	36.0	32.0	39.0	22.0	41.0
44	33.57400951193048	35.0	32.0	39.0	22.0	41.0
45	33.33478344640159	35.0	32.0	39.0	22.0	41.0
46	33.07983531583057	35.0	32.0	39.0	21.0	40.0
47	32.893817245190796	35.0	31.0	39.0	20.0	40.0
48	32.75642156713618	35.0	31.0	39.0	20.0	40.0
49	32.57172380923408	35.0	31.0	39.0	20.0	40.0
50	32.37883646172411	35.0	31.0	38.0	19.0	40.0
51	30.54354091245576	34.0	28.0	37.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	7.0
11	9.0
12	11.0
13	13.0
14	22.0
15	59.0
16	124.0
17	287.0
18	566.0
19	1049.0
20	1836.0
21	2730.0
22	3870.0
23	4665.0
24	5587.0
25	6324.0
26	7096.0
27	8292.0
28	10019.0
29	12182.0
30	15427.0
31	19588.0
32	24878.0
33	33861.0
34	54690.0
35	56587.0
36	49357.0
37	60424.0
38	67778.0
39	45721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.049080750002535	35.349294717734985	19.940981412187035	24.660643120075445
2	19.70510987395171	35.71435814750591	21.285226085810187	23.295305892732195
3	20.21356210641599	33.862675306501174	21.98858162716883	23.935180959914007
4	21.60972691227323	32.009572774380665	19.516696581586608	26.864003731759507
5	17.58044071268494	37.38756553395597	18.374250859420158	26.657742893938934
6	20.773934471114355	36.512630180604994	21.28056138642978	21.43287396185087
7	76.74241732834413	10.487258272235913	6.663016032368957	6.107308367050997
8	79.22484865078641	7.614006266922211	5.549369758551103	7.611775323740277
9	71.69338728159573	13.238619654609431	9.753886404429437	5.3141066593653985
10	42.583635017695435	29.23853852940282	12.828937361199841	15.348889091701906
11	34.844695932584955	24.755762424832426	24.856966120085588	15.542575522497035
12	33.94562583026578	23.79970186486569	26.650847251376593	15.603825053491931
13	23.709855698538732	36.0905762931865	21.039213896747892	19.16035411152688
14	15.241803818969101	37.24600204841147	29.520448622392585	17.99174551022685
15	15.071846511109083	29.829941285631712	34.5443298550901	20.553882348169104
16	15.634855445022461	26.097776155273642	36.53412836035817	21.733240039345723
17	15.517832334479225	27.488262196667783	28.17823207893483	28.815673389918167
18	19.046170383215195	28.899435165748937	26.961962418748037	25.09243203228783
19	22.035431433989434	32.39167249754089	26.538488840213763	19.03440722825591
20	24.87562491760721	30.387068642065447	24.267185867989006	20.47012057233833
21	18.130266800523255	33.22036648312089	25.251437437254726	23.39792927910113
22	17.915285002991492	30.84927950675874	26.20080516767566	25.034630322574102
23	20.38575035745794	31.39606339934897	21.862026304848246	26.35615993834484
24	17.465851358340178	32.52776003163883	27.192966444586414	22.813422165434577
25	18.215651080486346	29.051747741170033	26.853051828866377	25.879549349477248
26	19.689696084694717	35.806232443998255	22.146978593086104	22.357092878220925
27	18.39939967347104	33.51343129202032	24.64299838763652	23.444170646872116
28	22.47938912719418	29.81615000050703	28.48326285581009	19.221198016488696
29	18.587812965836147	30.92878220924219	27.860626895034123	22.62277792988754
30	25.210266394897225	29.29228397878576	24.077758510541205	21.41969111577581
31	28.625231967387666	31.572105097705172	19.107419914210094	20.695243020697067
32	27.26111161814365	29.2991796213481	20.226744952491053	23.212963808017196
33	30.758622088365634	27.727175930151198	21.201464309979414	20.312737671503758
34	21.862634743897864	29.72265319988237	23.660977761552736	24.753734294667034
35	25.699654203806798	30.399440236074348	22.1834849360632	21.717420624055652
36	29.032480504598784	27.036597608834533	26.431606380497502	17.49931550606918
37	24.630423980611077	30.62679362761502	23.654284932006934	21.088497459766966
38	27.408556681167795	27.432285804102907	23.89745773883768	21.261699775891614
39	23.170778700577003	26.59771024104327	27.484814375386613	22.746696682993115
40	25.925182278198616	27.82594586920589	22.915842738786978	23.33302911380852
41	20.256355652905803	24.445864135560218	31.082920101812135	24.21486010972184
42	25.758875604636305	26.599738371208666	24.03618184215063	23.605204182004403
43	23.437680630342854	25.62887246103455	26.905580400150082	24.027866508472513
44	23.005080466064314	25.95012827923296	24.275298388650583	26.76949286605214
45	24.634885866974944	29.452506261851884	23.381298611744903	22.53130925942827
46	21.558009593055683	27.492521270015107	29.61333698396763	21.336132152961575
47	22.204171863750215	26.795250119152648	26.188433573666757	24.812144443430377
48	24.424568768823583	21.937878373033982	31.93534321032724	21.702209647815195
49	22.28854207863061	22.130347925729872	29.34927443643333	26.23183555920619
50	20.249460010343466	24.75515398578281	26.28213318730796	28.713252816565767
51	20.521026639489722	24.629815541561456	29.501384198837883	25.347773620110935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2656.0
1	2309.5
2	1963.0
3	1471.5
4	980.0
5	1007.5
6	1035.0
7	1129.5
8	1224.0
9	1436.0
10	1648.0
11	1724.0
12	1800.0
13	1896.5
14	1993.0
15	2011.0
16	2029.0
17	2070.5
18	2112.0
19	2127.5
20	2143.0
21	2058.0
22	1973.0
23	2090.5
24	2208.0
25	2496.5
26	3071.5
27	3358.0
28	4336.0
29	5314.0
30	6125.0
31	6936.0
32	8406.0
33	9876.0
34	9976.5
35	10077.0
36	11124.5
37	12172.0
38	13155.0
39	14138.0
40	16720.5
41	19303.0
42	23880.0
43	28457.0
44	34454.5
45	40452.0
46	45985.5
47	51519.0
48	50117.5
49	48716.0
50	49068.0
51	49420.0
52	45726.0
53	42032.0
54	36381.5
55	30731.0
56	27112.0
57	23493.0
58	19886.0
59	16279.0
60	16156.5
61	16034.0
62	14223.5
63	12413.0
64	10771.5
65	9130.0
66	7575.5
67	6021.0
68	4804.0
69	3587.0
70	2614.5
71	1642.0
72	1863.0
73	2084.0
74	1726.0
75	1154.0
76	940.0
77	663.0
78	386.0
79	296.0
80	206.0
81	178.0
82	150.0
83	98.0
84	46.0
85	33.0
86	20.0
87	20.0
88	20.0
89	68.5
90	117.0
91	77.0
92	37.0
93	35.5
94	34.0
95	19.0
96	4.0
97	2.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	493065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.99272313380217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.79673936005341	25.529149893263632
2	9.217632807059964	5.8979434869064225
3	3.328373158584421	3.1945116284571
4	1.643262278010434	2.1028974038643544
5	0.9222413338601888	1.4752505828368716
6	0.5752760613243295	1.1042788653272084
7	0.40004185951962234	0.895889991747986
8	0.3024440309660042	0.774080651693317
9	0.21935139136478196	0.6315883499652978
>10	2.2970224847809906	17.288781832246393
>50	0.9483553383595722	22.14364930984191
>100	0.34033719804033036	14.1819267076191
>500	0.005098684614836409	1.1022888413352243
>1k	0.0038240134611273067	3.6777624548951984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	4476	0.9077910620303611	Illumina PCR Primer Index 8 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	3515	0.7128877531359963	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	3439	0.6974739638790017	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT	3128	0.6343991157352479	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2177	0.44152393700627707	No Hit
AAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1302	0.26406254753430075	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	957	0.19409205682820724	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	883	0.17908389360429153	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	732	0.1484591281068419	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	619	0.1255412572378895	No Hit
AACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	586	0.11884842769208928	TruSeq Adapter, Index 14 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	567	0.11499498037784064	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	549	0.11134434608013141	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	513	0.10404307748471298	TruSeq Adapter, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2464887996511615	0.0
2	0.0	0.0	0.0	1.5868090414042773	0.0
3	0.0	0.0	0.0	2.0790362325454046	0.0
4	0.0	0.0	0.0	4.73406143206271	0.0
5	0.0	0.0	0.0	5.131169318446858	0.0
6	0.0	0.0	0.0	6.946548629491041	0.0
7	0.0	0.0	0.0	8.391794185350816	0.0
8	0.0	0.0	0.0	9.283765832091104	0.0
9	0.0	0.0	0.0	11.29100625678156	0.0
10	0.0	0.0	0.0	13.258089704197216	0.0
11	0.0	0.0	0.0	16.083477837607617	0.0
12	0.0	0.0	0.0	17.172583736424205	0.0
13	0.0	0.0	0.0	17.629318649670935	0.0
14	0.0	0.0	0.0	18.153995923458368	0.0
15	0.0	0.0	0.0	18.49107115694685	0.0
16	0.0	0.0	0.0	19.12202245140093	0.0
17	0.0	0.0	0.0	19.855597132223945	0.0
18	0.0	0.0	0.0	20.780627300660157	0.0
19	0.0	0.0	0.0	21.21748653828603	0.0
20	0.0	0.0	0.0	21.587011854420815	0.0
21	0.0	0.0	0.0	22.054901483577215	0.0
22	0.0	0.0	0.0	22.51366452698934	0.0
23	0.0	0.0	0.0	22.976281017715717	0.0
24	0.0	0.0	0.0	23.316195633435754	0.0
25	0.0	0.0	0.0	23.614330767748672	0.0
26	0.0	0.0	0.0	23.88427489276262	0.0
27	0.0	0.0	0.0	24.169835620050094	0.0
28	0.0	0.0	0.0	24.43673754981595	0.0
29	0.0	0.0	0.0	24.71661951264032	0.0
30	0.0	0.0	0.0	25.06586352712117	0.0
31	0.0	0.0	0.0	25.348382059160556	0.0
32	0.0	0.0	0.0	25.616703680042185	0.0
33	0.0	0.0	0.0	25.91727257055358	0.0
34	0.0	0.0	0.0	26.174236662509	0.0
35	0.0	0.0	0.0	26.484946203847365	0.0
36	0.0	0.0	0.0	26.75042844249744	0.0
37	0.0	0.0	0.0	27.029904779288735	0.0
38	0.0	0.0	0.0	27.30897549004695	0.0
39	0.0	0.0	0.0	27.56391145183698	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCT	35	1.2100463E-7	45.000004	24
TAGAGTA	20	7.029362E-4	45.000004	28
TCGCAGG	80	0.0	45.000004	2
CGAACGG	40	6.8012014E-9	45.000004	2
GTCGATC	20	7.029362E-4	45.000004	34
CCCTCCG	20	7.029362E-4	45.000004	37
TTGTGCG	20	7.029362E-4	45.000004	24
GTCGAAC	20	7.029362E-4	45.000004	18
TCTCTAA	20	7.029362E-4	45.000004	4
ACGTTCC	20	7.029362E-4	45.000004	17
CGTGCCG	20	7.029362E-4	45.000004	13
TTTCGCG	20	7.029362E-4	45.000004	1
ACTCTTG	35	1.2100463E-7	45.000004	19
ATTACCG	20	7.029362E-4	45.000004	1
CGATTCG	20	7.029362E-4	45.000004	27
TGCGGTA	20	7.029362E-4	45.000004	12
CTAAGCG	20	7.029362E-4	45.000004	1
CACGAGA	35	1.2100463E-7	45.000004	30
ACTTAGC	20	7.029362E-4	45.000004	30
TCGATGG	20	7.029362E-4	45.000004	2
>>END_MODULE