##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564283
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.150536167135993	31.0	31.0	34.0	28.0	34.0
2	31.35936046274653	31.0	31.0	34.0	28.0	34.0
3	31.36394504176096	31.0	31.0	34.0	28.0	34.0
4	35.40586904088906	37.0	35.0	37.0	33.0	37.0
5	35.04864580361273	37.0	35.0	37.0	32.0	37.0
6	35.12350717636363	37.0	35.0	37.0	32.0	37.0
7	35.053944563277646	37.0	35.0	37.0	32.0	37.0
8	35.42195316888866	37.0	35.0	37.0	33.0	37.0
9	35.90345447231265	39.0	37.0	39.0	32.0	39.0
10	35.768779140962955	38.0	35.0	39.0	30.0	39.0
11	36.14096827301194	38.0	35.0	39.0	31.0	39.0
12	35.76712571528825	37.0	35.0	39.0	31.0	39.0
13	35.40720702200846	37.0	35.0	39.0	30.0	39.0
14	36.402998141003714	38.0	35.0	40.0	31.0	41.0
15	36.53376054213932	38.0	35.0	40.0	31.0	41.0
16	36.62612731554911	38.0	35.0	40.0	31.0	41.0
17	36.62023488214247	38.0	35.0	40.0	31.0	41.0
18	36.508969789981265	38.0	35.0	40.0	31.0	41.0
19	36.46841212653934	38.0	35.0	40.0	31.0	41.0
20	36.366989613367764	38.0	35.0	40.0	31.0	41.0
21	36.301866262141516	38.0	35.0	40.0	31.0	41.0
22	36.28308845029888	38.0	35.0	40.0	30.0	41.0
23	36.24211255699711	38.0	35.0	40.0	31.0	41.0
24	35.854395755321356	38.0	34.0	40.0	30.0	41.0
25	35.88807566416142	38.0	34.0	40.0	30.0	41.0
26	35.79374179268204	38.0	34.0	40.0	30.0	41.0
27	35.71300570812872	38.0	34.0	40.0	29.0	41.0
28	35.56893792653686	37.0	34.0	40.0	29.0	41.0
29	35.447856837792386	37.0	34.0	40.0	29.0	41.0
30	35.3837684282532	37.0	34.0	40.0	28.0	41.0
31	35.17160715456606	37.0	34.0	40.0	27.0	41.0
32	35.15523948089877	37.0	34.0	40.0	27.0	41.0
33	35.003742802813484	37.0	34.0	40.0	27.0	41.0
34	34.826703976550775	37.0	34.0	40.0	26.0	41.0
35	34.732077698601586	37.0	34.0	40.0	26.0	41.0
36	34.57646074753271	37.0	33.0	40.0	25.0	41.0
37	34.406110054706595	37.0	33.0	40.0	25.0	41.0
38	34.40376016998563	37.0	33.0	40.0	25.0	41.0
39	34.27498258852384	36.0	33.0	40.0	24.0	41.0
40	33.95703928702442	36.0	33.0	40.0	23.0	41.0
41	33.98144370821024	36.0	33.0	40.0	23.0	41.0
42	33.901877249536135	36.0	33.0	40.0	23.0	41.0
43	33.81388062372958	36.0	33.0	39.0	23.0	41.0
44	33.75360235909996	36.0	33.0	39.0	23.0	41.0
45	33.44631151390348	35.0	33.0	39.0	22.0	41.0
46	33.30941212122286	35.0	32.0	39.0	22.0	41.0
47	33.08336951494197	35.0	32.0	39.0	20.0	40.0
48	32.96908466141989	35.0	32.0	39.0	20.0	40.0
49	32.84487925384958	35.0	32.0	39.0	20.0	40.0
50	32.702782114648144	35.0	31.0	39.0	19.0	40.0
51	30.889255923003173	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	15.0
11	13.0
12	5.0
13	9.0
14	33.0
15	58.0
16	127.0
17	299.0
18	610.0
19	1127.0
20	1873.0
21	2773.0
22	4005.0
23	5096.0
24	6207.0
25	6919.0
26	7751.0
27	9315.0
28	11185.0
29	13571.0
30	16971.0
31	21661.0
32	27781.0
33	38054.0
34	60683.0
35	64127.0
36	56788.0
37	72653.0
38	82199.0
39	52367.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.391895555953308	33.16403294091794	20.347945977461666	26.096125525667084
2	20.279540585131926	33.91347958382585	20.987518674140457	24.819461156901767
3	20.64761830499944	33.752212985328285	20.353616890815424	25.24655181885685
4	19.379991954391677	34.91386414263765	19.244421682028342	26.46172222094233
5	17.70069273750937	36.195136128502895	19.031939647304633	27.0722314866831
6	21.328482339535306	36.293313815939875	19.806550968219845	22.571652876304974
7	78.5405550052013	8.881890824284977	6.493727438182614	6.083826732331118
8	81.19631461518424	7.252920963417292	5.529317735958729	6.021446685439753
9	72.88311007065603	12.534136240149001	9.327589170682087	5.255164518512874
10	43.36476555203683	27.843298486752214	12.2830211082028	16.508914853008154
11	35.591183856327405	25.295817878617644	21.7348387245407	17.378159540514247
12	33.405932838664285	24.67290348991552	25.346678882759182	16.57448478866101
13	23.30390956310929	34.45274800056	22.78289439873255	19.460448037598155
14	16.65405479165596	36.21870586212946	27.90567853364358	19.221560812571
15	15.595720587010419	28.353503472548347	34.60781203757689	21.442963902864342
16	16.757548960362087	25.88523843532412	34.599128451503944	22.75808415280985
17	16.91491680592894	26.465975405957646	27.64339879103216	28.975708997081252
18	20.866480117246134	27.15109262550883	26.536861822879654	25.44556543436538
19	23.49016362357186	30.873338378083336	25.438654008715485	20.197843989629316
20	27.325295995094663	28.723530568881216	23.27059294715595	20.680580488868173
21	20.200856662348503	32.30648451220398	23.85664639905863	23.636012426388888
22	19.40657436073743	29.85275119044876	24.703916297318898	26.036758151494904
23	22.126308961992475	30.034752065896015	21.22197549810999	26.61696347400152
24	18.452620405009544	29.971131506708517	28.104514933109805	23.47173315517214
25	19.38920718859154	29.21353292585458	26.806052991140973	24.59120689441291
26	21.198760196568035	32.79418306062738	22.609931541442858	23.397125201361728
27	18.934825256121485	30.46733642516255	24.415585796488642	26.182252522227323
28	18.994015414251358	30.83771795358003	28.848467878706252	21.319798753462358
29	19.362447566203482	27.796159019499083	26.10073314276702	26.74066027153042
30	22.02175149703252	29.61191458895625	23.51568273366378	24.850651180347448
31	24.890879221950687	29.505939395657855	21.678129591003096	23.925051791388363
32	25.550831763494557	26.46172222094233	20.961999564048536	27.02544645151458
33	24.85773982203965	28.739480012688666	22.22289170504871	24.179888460222973
34	18.707811505928763	27.95547624153129	25.988200955903334	27.34851129663662
35	21.758054026082657	26.157087844220012	25.906681576443024	26.178176553254307
36	25.579186330263358	25.614275106639756	26.510456632576208	22.29608193052068
37	21.027037851574477	28.01005878256123	26.822002434948423	24.14090093091587
38	22.25266400015595	25.928656365688845	27.31324530421792	24.505434329937284
39	22.676919205434153	25.155108341027464	28.138717629274673	24.029254824263713
40	25.054272412955907	24.11892614167005	24.95343648488436	25.87336496048968
41	20.119691714972806	21.643749678795924	30.967617312589606	27.268941293641664
42	24.96849984848028	23.02532594460581	26.357696404109287	25.64847780280462
43	21.413723255884015	22.900034202696165	28.60497303657916	27.081269504840655
44	22.51671590319042	23.624670599681366	26.60119124623637	27.25742225089184
45	22.844211149370086	26.520735163029897	25.463641470680493	25.171412216919524
46	20.966429965106162	25.66442724661207	29.285482639030413	24.08366014925135
47	22.09281512999683	24.639232441877567	26.73605265443049	26.531899773695113
48	23.120490959323604	21.14701311221497	32.57266300774611	23.159832920715314
49	22.412335654272766	21.108911663119393	30.12194235871008	26.35681032389776
50	21.08516471345052	24.263357216148634	26.309316424560013	28.34216164584083
51	20.825897643558285	23.992216671421964	28.850771687256216	26.331113997763534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2755.0
1	2397.0
2	2039.0
3	1537.5
4	1036.0
5	1026.0
6	1016.0
7	1116.5
8	1217.0
9	1338.5
10	1460.0
11	1674.5
12	1889.0
13	1969.0
14	2049.0
15	2154.0
16	2259.0
17	2220.0
18	2181.0
19	2189.5
20	2198.0
21	2192.0
22	2186.0
23	2335.0
24	2484.0
25	2235.0
26	2798.0
27	3610.0
28	3925.5
29	4241.0
30	4941.5
31	5642.0
32	6971.0
33	8300.0
34	9058.5
35	9817.0
36	10902.5
37	11988.0
38	13584.5
39	15181.0
40	17687.0
41	20193.0
42	28891.0
43	37589.0
44	44261.0
45	50933.0
46	53866.0
47	56799.0
48	56787.5
49	56776.0
50	53433.0
51	50090.0
52	46084.5
53	42079.0
54	37423.5
55	32768.0
56	29386.0
57	26004.0
58	23750.5
59	21497.0
60	21100.5
61	20704.0
62	18879.5
63	17055.0
64	15364.0
65	13673.0
66	11901.5
67	10130.0
68	8633.0
69	7136.0
70	6474.0
71	5812.0
72	4505.5
73	3199.0
74	2853.0
75	1994.5
76	1482.0
77	1260.5
78	1039.0
79	846.5
80	654.0
81	492.0
82	330.0
83	245.0
84	160.0
85	112.5
86	65.0
87	53.0
88	41.0
89	29.5
90	18.0
91	14.5
92	11.0
93	6.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	564283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.859768168399082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52482586562006	24.541176898453422
2	9.219545902988486	5.690260983682766
3	3.49966662332616	3.23996701987588
4	1.677342109556772	2.0704955456006178
5	0.948951648927331	1.4642213944458735
6	0.6017964372958364	1.114277912371082
7	0.42235271910623434	0.9123594897837959
8	0.2714417323187974	0.6701303144469383
9	0.21836594509880386	0.6064850197460205
>10	2.1395459844114946	16.47525569855818
>50	1.0633001241443054	23.793303470918254
>100	0.40594538362125326	16.20801021116526
>500	0.002883134826855492	0.599143322438556
>1k	0.004036388757597689	2.6149127185133887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	4999	0.8859029954827631	TruSeq Adapter, Index 14 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2271	0.4024576320746859	No Hit
AAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1654	0.29311533397249256	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	1612	0.2856722601956819	No Hit
AATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1589	0.28159629122266666	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGT	1378	0.24420370629630878	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	1192	0.21124152242757624	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	869	0.15400074076305684	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	698	0.1236967975288995	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	661	0.117139803963614	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	579	0.1026080884946029	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.772160423050136E-4	0.0	0.0	1.247955369911906	0.0
2	1.772160423050136E-4	0.0	0.0	1.5759822642184862	0.0
3	1.772160423050136E-4	0.0	0.0	1.9607182920626707	0.0
4	1.772160423050136E-4	0.0	0.0	4.071184139873078	0.0
5	1.772160423050136E-4	0.0	0.0	4.369084306987806	0.0
6	1.772160423050136E-4	0.0	0.0	6.132738360007301	0.0
7	1.772160423050136E-4	0.0	0.0	7.579530129385432	0.0
8	1.772160423050136E-4	0.0	0.0	8.52745873967495	0.0
9	1.772160423050136E-4	0.0	0.0	10.033086235098345	0.0
10	1.772160423050136E-4	0.0	0.0	12.118919053028357	0.0
11	1.772160423050136E-4	0.0	0.0	14.450196089550811	0.0
12	1.772160423050136E-4	0.0	0.0	15.520403769030787	0.0
13	1.772160423050136E-4	0.0	0.0	15.946608350774346	0.0
14	1.772160423050136E-4	0.0	0.0	16.358635649133504	0.0
15	1.772160423050136E-4	0.0	0.0	16.688789135947744	0.0
16	1.772160423050136E-4	0.0	0.0	17.284057822050283	0.0
17	1.772160423050136E-4	0.0	0.0	18.004795466104774	0.0
18	1.772160423050136E-4	0.0	0.0	18.918875812314035	0.0
19	1.772160423050136E-4	0.0	0.0	19.31211821018886	0.0
20	1.772160423050136E-4	0.0	0.0	19.65609454830289	0.0
21	1.772160423050136E-4	0.0	0.0	20.079640889411873	0.0
22	1.772160423050136E-4	0.0	0.0	20.50690876740926	0.0
23	1.772160423050136E-4	0.0	0.0	20.9148600967954	0.0
24	1.772160423050136E-4	0.0	0.0	21.234026188986732	0.0
25	1.772160423050136E-4	0.0	0.0	21.47149568567545	0.0
26	1.772160423050136E-4	0.0	0.0	21.70754745402573	0.0
27	1.772160423050136E-4	0.0	0.0	21.955118265125833	0.0
28	1.772160423050136E-4	0.0	0.0	22.197549810999092	0.0
29	1.772160423050136E-4	0.0	0.0	22.447247214606854	0.0
30	1.772160423050136E-4	0.0	0.0	22.785729855409432	0.0
31	1.772160423050136E-4	0.0	0.0	23.052617215120783	0.0
32	1.772160423050136E-4	0.0	0.0	23.283175286159604	0.0
33	1.772160423050136E-4	0.0	0.0	23.511961196775378	0.0
34	1.772160423050136E-4	0.0	0.0	23.724088799414478	0.0
35	1.772160423050136E-4	0.0	0.0	24.00479901042562	0.0
36	1.772160423050136E-4	0.0	0.0	24.248825500679622	0.0
37	1.772160423050136E-4	0.0	0.0	24.50437103368345	0.0
38	1.772160423050136E-4	0.0	0.0	24.743081042668308	0.0
39	1.772160423050136E-4	0.0	0.0	25.005006353195117	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	7.030229E-4	45.000004	18
CTTAATA	20	7.030229E-4	45.000004	41
TTGGCGT	20	7.030229E-4	45.000004	37
CGAACAC	20	7.030229E-4	45.000004	21
CTCGTTA	20	7.030229E-4	45.000004	29
CTCCGTA	20	7.030229E-4	45.000004	22
CTGTACG	20	7.030229E-4	45.000004	1
GTCGAGT	20	7.030229E-4	45.000004	15
CGAGTCA	20	7.030229E-4	45.000004	17
CCGATTC	20	7.030229E-4	45.000004	34
GCGAATT	20	7.030229E-4	45.000004	20
TTAGATC	20	7.030229E-4	45.000004	12
TAGCGTG	20	7.030229E-4	45.000004	1
ATGTGCG	20	7.030229E-4	45.000004	32
TAGCGAA	20	7.030229E-4	45.000004	15
GTGATAG	20	7.030229E-4	45.000004	9
CACGAAT	20	7.030229E-4	45.000004	42
TGTGCGA	20	7.030229E-4	45.000004	43
CGTTAGA	20	7.030229E-4	45.000004	23
ACGATAT	20	7.030229E-4	45.000004	34
>>END_MODULE