##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954201
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49368424472412	31.0	31.0	34.0	30.0	34.0
2	31.676765167925836	31.0	31.0	34.0	30.0	34.0
3	31.876543830911935	33.0	31.0	34.0	30.0	34.0
4	35.70566369140254	37.0	35.0	37.0	35.0	37.0
5	35.318541900501046	37.0	35.0	37.0	33.0	37.0
6	35.33964122863003	37.0	35.0	37.0	33.0	37.0
7	35.372464501714	37.0	35.0	37.0	33.0	37.0
8	35.66127681693899	37.0	35.0	37.0	35.0	37.0
9	36.53310151634719	39.0	37.0	39.0	33.0	39.0
10	36.191446037050895	38.0	35.0	39.0	31.0	39.0
11	36.41721817520627	38.0	35.0	39.0	32.0	39.0
12	36.142107375699666	37.0	35.0	39.0	32.0	39.0
13	35.95718302537935	38.0	35.0	39.0	31.0	39.0
14	37.02227622901255	39.0	35.0	40.0	32.0	41.0
15	36.991884309490345	39.0	35.0	40.0	32.0	41.0
16	37.01695764309616	39.0	35.0	40.0	32.0	41.0
17	36.96948546480249	38.0	35.0	40.0	32.0	41.0
18	36.84457467556626	38.0	35.0	40.0	31.0	41.0
19	36.906068008731914	39.0	35.0	40.0	31.0	41.0
20	36.87005568009256	39.0	35.0	40.0	31.0	41.0
21	36.874579884112464	39.0	35.0	40.0	31.0	41.0
22	36.818453344735545	39.0	35.0	40.0	31.0	41.0
23	36.78977280468161	39.0	35.0	40.0	31.0	41.0
24	36.477492687599366	38.0	35.0	40.0	31.0	41.0
25	36.569190348783955	38.0	35.0	40.0	31.0	41.0
26	36.46448809003554	38.0	35.0	40.0	31.0	41.0
27	36.29687350987894	38.0	35.0	40.0	30.0	41.0
28	36.246813826436984	38.0	35.0	40.0	30.0	41.0
29	36.05159080738754	38.0	35.0	40.0	30.0	41.0
30	35.94983551683555	38.0	35.0	40.0	30.0	41.0
31	35.84935354291182	38.0	34.0	40.0	29.0	41.0
32	35.75735301052923	38.0	34.0	40.0	29.0	41.0
33	35.72389150713529	38.0	34.0	40.0	29.0	41.0
34	35.5939450912334	38.0	34.0	40.0	29.0	41.0
35	35.48950483179121	38.0	34.0	40.0	28.0	41.0
36	35.284047071843354	38.0	34.0	40.0	27.0	41.0
37	35.10080475706901	37.0	34.0	40.0	27.0	41.0
38	35.09985946357214	37.0	34.0	40.0	27.0	41.0
39	35.13072612583722	37.0	34.0	40.0	27.0	41.0
40	35.02192305394775	37.0	34.0	40.0	27.0	41.0
41	35.04490563308988	37.0	34.0	40.0	27.0	41.0
42	34.953702626595444	37.0	34.0	40.0	27.0	41.0
43	34.8717995474748	37.0	33.0	40.0	26.0	41.0
44	34.6806438056552	37.0	33.0	40.0	26.0	41.0
45	34.530026692489315	36.0	33.0	40.0	26.0	41.0
46	34.438402391110465	36.0	33.0	40.0	25.0	41.0
47	34.302950845786164	36.0	33.0	40.0	24.0	41.0
48	34.24745729673308	36.0	33.0	40.0	24.0	41.0
49	34.08230760604946	36.0	33.0	39.0	24.0	41.0
50	33.87666959057893	35.0	33.0	39.0	24.0	41.0
51	31.92773535135679	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	9.0
10	20.0
11	22.0
12	26.0
13	42.0
14	62.0
15	117.0
16	199.0
17	420.0
18	697.0
19	1200.0
20	1984.0
21	2939.0
22	4207.0
23	5483.0
24	7000.0
25	8462.0
26	10199.0
27	12387.0
28	15386.0
29	19613.0
30	24560.0
31	31548.0
32	41044.0
33	56692.0
34	91945.0
35	96607.0
36	98260.0
37	131003.0
38	168231.0
39	123826.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.350533063788447	31.183262226721624	20.51559367470795	26.95061103478198
2	22.852103487629964	30.648993241465895	21.125318460156716	25.373584810747424
3	21.518841418107925	30.987181945942204	21.271514073030733	26.222462562919134
4	21.134226436568397	32.11294056493338	19.55458021947158	27.198252779026642
5	19.061183125987082	34.52333418221109	18.279377196209186	28.136105495592652
6	20.96350768863164	36.98518446323154	19.442025317516958	22.609282530619858
7	83.95600088450966	6.24061387485446	5.25203809260313	4.551347148032752
8	86.30477226496305	4.875911888585319	4.173020149842643	4.646295696608995
9	78.78182898571686	9.751299778558186	7.246586411039184	4.220284824685784
10	45.0701686541934	28.826211668191505	11.666724306514038	14.436895371101057
11	35.18483003057008	25.432063055897032	21.36143223492744	18.02167467860545
12	34.0537266257319	22.277486609215458	25.148579806560672	18.520206958491976
13	26.746985173983262	27.286913344253467	23.3732725075744	22.592828974188876
14	20.951036521655293	29.183473922161053	27.67215712412793	22.19333243205572
15	20.933010969386952	26.570188042142064	30.746142584214436	21.750658404256544
16	22.834077935361627	23.618818257369252	30.66282680483462	22.884277002434498
17	21.8455021531103	25.44830701288303	26.958785413136226	25.747405420870447
18	24.41183775745362	25.653714468964083	25.70359913686949	24.23084863671281
19	26.088738117021464	28.353879318927568	24.244786999804024	21.312595564246948
20	29.127301270906237	26.498924230848637	23.1065572138365	21.26721728440863
21	25.466018165983893	28.596071477602724	23.365307728665137	22.572602627748243
22	23.620809452096573	26.64690143900499	24.987293033648047	24.74499607525039
23	25.030365719591575	26.484252269699986	22.610749726734724	25.874632283973714
24	23.356714151420928	26.41194046118166	26.600370362219284	23.630975025178135
25	24.42147933192273	25.1699589499487	24.544304606681404	25.86425711144717
26	25.747929419482894	28.23681802890586	22.536341923766585	23.478910627844655
27	22.995679107441724	26.70726607915942	25.077315995267245	25.21973881813161
28	23.202344160192663	27.939082017310817	26.67268217073761	22.185891651758908
29	24.899156467033677	27.249919042214376	24.570399737581493	23.280524753170454
30	25.562538710397494	26.24394650602965	25.101839130329985	23.09167565324287
31	28.028580980317564	27.140403332211978	21.0105627640298	23.82045292344066
32	28.182741372100846	25.303054597511426	24.755895246389386	21.75830878399834
33	27.675301115802647	25.83585638665229	24.198675121908277	22.290167375636788
34	24.408693765778906	25.925040950491564	25.296557014717024	24.369708269012506
35	25.596703419929344	25.539587571172113	26.598169568046988	22.265539440851562
36	26.601313559721696	27.3071396906941	24.946421141876815	21.145125607707392
37	24.053841905426633	29.689342182621903	24.13422329257672	22.122592619374746
38	24.770357608093054	28.037174557561773	25.105716720062127	22.08675111428305
39	24.178553575190133	25.911731385735294	26.174359490296066	23.735355548778507
40	24.95134672883386	24.657069108081	24.811124700141793	25.580459462943345
41	21.099432928701606	24.0238691847944	27.22843509910386	27.648262787400142
42	24.61242442630012	25.70800072521408	24.461198426746567	25.218376421739237
43	23.62342944515883	24.501441520182855	26.448410764608294	25.42671827005002
44	24.031309965091214	23.86792719772878	26.252330483828878	25.848432353351132
45	25.390247966623384	24.70380978431169	25.866667505064445	24.039274744000476
46	22.00951371880767	24.692491414282735	28.7199447495863	24.57805011732329
47	23.58591114450729	24.995153012834823	25.73399105639168	25.684944786266207
48	23.35524695530606	22.65602320685055	29.448302820894128	24.54042701694926
49	23.600583105655936	23.008150274418075	27.80567197058062	25.585594649345367
50	21.37882898886084	25.068408018855564	26.18064747364549	27.372115518638108
51	22.073231950081794	24.30986762747052	27.969893135722977	25.647007286724705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1921.0
1	1687.5
2	1454.0
3	1122.0
4	790.0
5	824.0
6	858.0
7	899.5
8	941.0
9	1059.5
10	1178.0
11	1308.5
12	1439.0
13	1585.0
14	1731.0
15	1674.5
16	1618.0
17	1671.0
18	1724.0
19	1811.5
20	1899.0
21	1800.0
22	1701.0
23	2557.5
24	3414.0
25	3492.5
26	4362.5
27	5154.0
28	6510.5
29	7867.0
30	8636.5
31	9406.0
32	11642.5
33	13879.0
34	16990.0
35	20101.0
36	20995.5
37	21890.0
38	25214.0
39	28538.0
40	33834.5
41	39131.0
42	48339.5
43	57548.0
44	65862.5
45	74177.0
46	78219.0
47	82261.0
48	79082.0
49	75903.0
50	70984.5
51	66066.0
52	61336.5
53	56607.0
54	54776.5
55	52946.0
56	52443.5
57	51941.0
58	51481.5
59	51022.0
60	51807.5
61	52593.0
62	49718.0
63	46843.0
64	40908.5
65	34974.0
66	29588.0
67	24202.0
68	20514.5
69	16827.0
70	14862.0
71	12897.0
72	11246.0
73	9595.0
74	8003.5
75	5635.5
76	4859.0
77	3816.0
78	2773.0
79	2193.5
80	1614.0
81	1221.0
82	828.0
83	680.5
84	533.0
85	384.5
86	236.0
87	196.0
88	156.0
89	139.5
90	123.0
91	78.0
92	33.0
93	24.0
94	15.0
95	11.0
96	7.0
97	5.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	954201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.087341964090484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2984854509537	21.991963361313
2	9.693110396621757	5.445074128111921
3	3.2194512069574333	2.712774809595518
4	1.4512516279514207	1.6304720300085829
5	0.8211944438753795	1.153258458206945
6	0.4678907191606605	0.7885083978533818
7	0.32270871796893336	0.6344821081470663
8	0.2916042391964819	0.655231038759202
9	0.22299094312189688	0.5636900586923792
>10	3.494128386357494	24.992703441696815
>50	1.4983054624383316	29.390096046559865
>100	0.21372832526959712	8.053090241811487
>500	0.0029429029297018527	0.5909036719174745
>1k	0.0022071771972763895	1.397752207326385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	4858	0.5091170518580467	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	2453	0.25707371926879136	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC	2010	0.21064744220557302	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGT	1702	0.1783691276785499	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1499	0.15709478401301194	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	1006	0.10542852082527686	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0479972249033485E-4	0.0	0.0	0.6871717803691256	0.0
2	1.0479972249033485E-4	0.0	0.0	0.8983432211871503	0.0
3	1.0479972249033485E-4	0.0	0.0	1.190210448322733	0.0
4	1.0479972249033485E-4	0.0	0.0	2.614648276411364	0.0
5	1.0479972249033485E-4	0.0	0.0	2.789663812970223	0.0
6	1.0479972249033485E-4	0.0	0.0	3.8578873843142065	0.0
7	1.0479972249033485E-4	0.0	0.0	4.7161971115100485	0.0
8	1.0479972249033485E-4	0.0	0.0	5.240614922851685	0.0
9	1.0479972249033485E-4	0.0	0.0	6.214099545064404	0.0
10	1.0479972249033485E-4	0.0	0.0	7.405986788946983	0.0
11	1.0479972249033485E-4	0.0	0.0	8.903050824721417	0.0
12	1.0479972249033485E-4	0.0	0.0	9.545682723032149	0.0
13	1.0479972249033485E-4	0.0	0.0	9.815961207334723	0.0
14	1.0479972249033485E-4	0.0	0.0	10.140001949274838	0.0
15	2.095994449806697E-4	0.0	0.0	10.366788548743923	0.0
16	2.095994449806697E-4	0.0	0.0	10.825706533529099	0.0
17	2.095994449806697E-4	0.0	0.0	11.35777472461253	0.0
18	2.095994449806697E-4	0.0	0.0	11.939517984156378	0.0
19	2.095994449806697E-4	0.0	0.0	12.257270742747073	0.0
20	2.095994449806697E-4	0.0	0.0	12.574185103557845	0.0
21	2.095994449806697E-4	0.0	0.0	12.94171773033145	0.0
22	2.095994449806697E-4	0.0	0.0	13.324970315478605	0.0
23	2.095994449806697E-4	0.0	0.0	13.71136689230047	0.0
24	2.095994449806697E-4	0.0	0.0	14.01853487891964	0.0
25	2.095994449806697E-4	0.0	0.0	14.275398998743452	0.0
26	2.095994449806697E-4	0.0	0.0	14.501871199045064	0.0
27	2.095994449806697E-4	0.0	0.0	14.741548164380461	0.0
28	2.095994449806697E-4	0.0	0.0	14.975565944701378	0.0
29	2.095994449806697E-4	0.0	0.0	15.22331248866853	0.0
30	2.095994449806697E-4	0.0	0.0	15.53320526807245	0.0
31	3.1439916747100455E-4	0.0	0.0	15.791326984566146	0.0
32	3.1439916747100455E-4	0.0	0.0	16.045571111327696	0.0
33	3.1439916747100455E-4	0.0	0.0	16.310504809783264	0.0
34	3.1439916747100455E-4	0.0	0.0	16.57292331489906	0.0
35	3.1439916747100455E-4	0.0	0.0	16.854520169230593	0.0
36	3.1439916747100455E-4	0.0	0.0	17.11966346713114	0.0
37	3.1439916747100455E-4	0.0	0.0	17.386797959759004	0.0
38	3.1439916747100455E-4	0.0	0.0	17.66451722435839	0.0
39	3.1439916747100455E-4	0.0	0.0	17.953869258154203	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	35	1.211647E-7	45.000004	19
ACCGGAC	35	1.211647E-7	45.000004	34
TCCGATC	20	7.032683E-4	45.000004	17
GCACGTA	20	7.032683E-4	45.000004	39
CGATTGT	20	7.032683E-4	45.000004	12
TAACGTG	20	7.032683E-4	45.000004	45
TTATACG	20	7.032683E-4	45.000004	1
TACGCTT	35	1.211647E-7	45.000004	23
CTAACCG	40	6.8121153E-9	45.000004	1
CGTAGAT	20	7.032683E-4	45.000004	34
TTGCGCA	20	7.032683E-4	45.000004	10
AACTCGT	20	7.032683E-4	45.000004	20
CACGTAT	20	7.032683E-4	45.000004	40
CGATATA	20	7.032683E-4	45.000004	21
TGTCGAT	20	7.032683E-4	45.000004	18
TAATGCG	20	7.032683E-4	45.000004	1
TCGAACA	20	7.032683E-4	45.000004	14
GTTATAG	20	7.032683E-4	45.000004	1
ATCGATC	20	7.032683E-4	45.000004	30
TCTAGCG	25	3.890307E-5	45.0	1
>>END_MODULE