FastQCFastQC Report
Sat 18 Jun 2016
SRR3552902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981524
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT47030.4791528276435421No Hit
AATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT22240.22658641052078196No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC18710.19062193079333772No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGT17450.17778475106059557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15880.16178921758408352No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT207.032782E-445.00000431
CGAACTA207.032782E-445.00000413
ATCTACG207.032782E-445.0000041
CAACGAC207.032782E-445.00000410
ACGCAAC207.032782E-445.00000426
CGATTAT207.032782E-445.00000428
TAACGCA207.032782E-445.00000424
TCGATAG207.032782E-445.0000041
GTAATCG207.032782E-445.0000041
ATATACG207.032782E-445.0000041
ATGCGTC207.032782E-445.00000437
ATGCGAA207.032782E-445.00000412
CGCAACA207.032782E-445.00000427
CGATACG207.032782E-445.0000041
CTCGTTA551.8189894E-1245.014
CGTTATG453.8380676E-1045.01
GTTACGG950.045.02
TCTCGAC351.2116834E-745.024
GATATCG253.8903887E-545.036
CGTACTA253.8903887E-545.034