##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	981524
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27411352142179	31.0	31.0	34.0	28.0	34.0
2	31.49305569705886	31.0	31.0	34.0	30.0	34.0
3	31.45748550213749	31.0	31.0	34.0	28.0	34.0
4	35.482829762695566	37.0	35.0	37.0	33.0	37.0
5	35.175294745721956	37.0	35.0	37.0	32.0	37.0
6	35.23654235658017	37.0	35.0	37.0	32.0	37.0
7	35.223741854503814	37.0	35.0	37.0	33.0	37.0
8	35.58033934982741	37.0	35.0	37.0	35.0	37.0
9	36.04893614420024	39.0	37.0	39.0	32.0	39.0
10	35.87138572261096	38.0	35.0	39.0	30.0	39.0
11	36.246886474502915	38.0	35.0	39.0	32.0	39.0
12	36.0361600938948	37.0	35.0	39.0	32.0	39.0
13	35.831032150003466	37.0	35.0	39.0	31.0	39.0
14	36.89598114768462	39.0	35.0	40.0	31.0	41.0
15	36.89270868567656	38.0	35.0	40.0	31.0	41.0
16	36.92881070661543	38.0	35.0	40.0	32.0	41.0
17	36.885783740387396	38.0	35.0	40.0	32.0	41.0
18	36.79513491264605	38.0	35.0	40.0	31.0	41.0
19	36.83559342410374	39.0	35.0	40.0	31.0	41.0
20	36.7844515264018	38.0	35.0	40.0	31.0	41.0
21	36.77806757654423	38.0	35.0	40.0	31.0	41.0
22	36.741685379063576	38.0	35.0	40.0	31.0	41.0
23	36.72361857682542	38.0	35.0	40.0	31.0	41.0
24	36.35621849287435	38.0	35.0	40.0	30.0	41.0
25	36.49956292459481	38.0	35.0	40.0	31.0	41.0
26	36.38548013089848	38.0	35.0	40.0	30.0	41.0
27	36.232471136722076	38.0	35.0	40.0	30.0	41.0
28	36.15458918987208	38.0	35.0	40.0	30.0	41.0
29	35.97025849597157	38.0	35.0	40.0	30.0	41.0
30	35.91046882195443	38.0	34.0	40.0	30.0	41.0
31	35.86532881518944	38.0	34.0	40.0	30.0	41.0
32	35.76396399884262	38.0	34.0	40.0	29.0	41.0
33	35.6740446489337	38.0	34.0	40.0	29.0	41.0
34	35.616498424898424	38.0	34.0	40.0	29.0	41.0
35	35.46847962963717	38.0	34.0	40.0	28.0	41.0
36	35.31193735456291	38.0	34.0	40.0	27.0	41.0
37	35.13136510161748	37.0	34.0	40.0	27.0	41.0
38	35.131392609859766	37.0	34.0	40.0	27.0	41.0
39	35.116939575598764	37.0	34.0	40.0	27.0	41.0
40	34.960223081656686	37.0	34.0	40.0	26.0	41.0
41	34.980787021	37.0	34.0	40.0	27.0	41.0
42	34.94943475656224	37.0	34.0	40.0	27.0	41.0
43	34.891794800738445	37.0	33.0	40.0	27.0	41.0
44	34.79781849450446	37.0	33.0	40.0	26.0	41.0
45	34.66047901019231	37.0	33.0	40.0	26.0	41.0
46	34.485533721029746	36.0	33.0	40.0	26.0	41.0
47	34.31721486178637	36.0	33.0	40.0	25.0	41.0
48	34.21595294664216	36.0	33.0	39.0	24.0	41.0
49	34.05541790114149	36.0	33.0	39.0	24.0	41.0
50	33.93982928588603	35.0	33.0	39.0	24.0	40.0
51	32.112864280445514	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	19.0
10	19.0
11	27.0
12	28.0
13	38.0
14	64.0
15	138.0
16	228.0
17	408.0
18	788.0
19	1311.0
20	2108.0
21	2965.0
22	4204.0
23	5579.0
24	7020.0
25	8435.0
26	10334.0
27	12736.0
28	16333.0
29	20560.0
30	26086.0
31	33563.0
32	44082.0
33	61072.0
34	96014.0
35	100226.0
36	104609.0
37	138211.0
38	168669.0
39	115636.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.60966007963127	30.957572102159496	20.528789922610144	26.90397789559909
2	22.90193617272731	30.477400450727643	21.17452043964284	25.446142936902206
3	21.891364857099777	30.929045036086737	21.10462912776458	26.074960979048907
4	21.178799499553755	32.21979289350031	19.445780235633567	27.15562737131237
5	19.179867226883907	34.51051629914297	18.155439907735317	28.15417656623781
6	21.04849193702854	37.02955811574653	19.301209140071972	22.62074080715296
7	84.13039314372344	6.286754068163387	5.062739168884307	4.520113619228873
8	86.55641634845404	4.90308948125568	4.07417444708433	4.466319723205953
9	78.20471022613813	10.712422722215656	6.934216585636214	4.148650466010001
10	45.270721857030495	28.858693215856157	11.573940117613018	14.296644809500329
11	35.360419103353564	25.522452838646835	21.177780675765444	17.939347382234157
12	34.17807409701648	22.360125682102527	24.97860470044543	18.483195520435565
13	26.85324047094111	27.339728829860505	23.32454428011949	22.4824864190789
14	21.091791948031833	29.16668364706314	27.478085100313386	22.263439304591635
15	21.037182992978266	26.512647678508117	30.77245181982305	21.677717508690566
16	22.965408894739202	23.467790904756278	30.791809471800995	22.774990728703525
17	21.996711236811326	25.326940553669598	26.905302366523898	25.77104584299518
18	24.421715617753616	25.605385095015503	25.74435265974138	24.228546627489497
19	26.256821025262756	28.244342471503497	24.162934375522145	21.335902127711602
20	29.216809777448134	26.478109552084312	22.982321369625193	21.322759300842364
21	25.57156014524352	28.458601114185694	23.41746100961362	22.552377730957165
22	23.7720116879465	26.55044604105452	24.990932468284015	24.686609802714962
23	25.112987558123894	26.463234724774942	22.617582453409188	25.806195263691972
24	23.440180780092998	26.249994905881056	26.783349159062848	23.526475154963098
25	24.52400552610023	25.24543465060457	24.54295564856285	25.687604174732353
26	25.60497756550018	28.378419682045475	22.619416336228152	23.397186416226194
27	22.97243877887856	26.72018208418745	25.188788047974377	25.11859108895962
28	23.13708070306992	27.986172523545015	26.807189635709367	22.069557137675698
29	24.867247260382833	27.329133062462045	24.61366201947176	23.189957657683358
30	25.546598962429854	26.277503148165504	25.014365415415213	23.16153247398943
31	27.99014593631944	27.176717023730447	21.132748664322012	23.700388375628105
32	28.248112119520258	25.458470704740794	24.61753354986735	21.675883625871602
33	27.772525175135808	25.93986494471862	24.100378594919736	22.187231285225835
34	24.48325257456771	26.06650474160591	25.288836543986697	24.161406139839677
35	25.50360459856305	25.60966415492642	26.584169108447682	22.302562138062846
36	26.49981049877537	27.490820397667303	24.76954205908363	21.239827044473696
37	24.041999991849412	29.83951487686496	24.12961883764432	21.98886629364132
38	24.78869594630391	28.03558547727819	25.013550356384563	22.162168220033333
39	24.293547585183855	26.121521226174803	25.962992244713323	23.621938943928015
40	25.041669892941997	24.74559970005828	24.811008187267962	25.401722219731766
41	21.124292426879016	24.33022524156312	27.08991323696619	27.455569094591674
42	24.39736572921294	25.823311503335628	24.484373280734857	25.29494948671658
43	23.691015196775627	24.762104645428945	26.377144114662503	25.16973604313292
44	24.0507618764289	24.141437193588747	26.17939041735098	25.628410512631376
45	25.268663832978106	25.06418589866371	25.743028188816574	23.92412207954161
46	22.208626584780404	24.87947314584259	28.470725117266614	24.441175152110393
47	23.509460797698274	25.359644797274445	25.69341147032574	25.437482934701546
48	23.40869912503413	22.865971692999864	29.217624836478784	24.507704345487223
49	23.66819354391742	23.270240972202412	27.607068191913797	25.454497291966373
50	21.4388033303312	25.46539870650132	26.103386162742837	26.992411800424648
51	22.045614778650343	24.688443685533926	27.729428928890176	25.536512606925555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2071.0
1	1813.5
2	1556.0
3	1173.0
4	790.0
5	828.0
6	866.0
7	950.0
8	1034.0
9	1090.5
10	1147.0
11	1307.5
12	1468.0
13	1602.5
14	1737.0
15	1643.0
16	1549.0
17	1673.0
18	1797.0
19	1828.5
20	1860.0
21	1836.5
22	1813.0
23	2742.0
24	3671.0
25	3740.0
26	4594.5
27	5380.0
28	6863.0
29	8346.0
30	9132.0
31	9918.0
32	12279.0
33	14640.0
34	17731.0
35	20822.0
36	22168.0
37	23514.0
38	26662.5
39	29811.0
40	35089.0
41	40367.0
42	49404.0
43	58441.0
44	67311.0
45	76181.0
46	79847.5
47	83514.0
48	80233.5
49	76953.0
50	72201.0
51	67449.0
52	62723.5
53	57998.0
54	56242.0
55	54486.0
56	54129.0
57	53772.0
58	53010.5
59	52249.0
60	53303.0
61	54357.0
62	51261.0
63	48165.0
64	42265.0
65	36365.0
66	30633.5
67	24902.0
68	21293.0
69	17684.0
70	15310.5
71	12937.0
72	11421.0
73	9905.0
74	8375.0
75	5907.5
76	4970.0
77	3914.5
78	2859.0
79	2255.0
80	1651.0
81	1211.0
82	771.0
83	647.0
84	523.0
85	392.0
86	261.0
87	210.5
88	160.0
89	132.0
90	104.0
91	67.5
92	31.0
93	22.5
94	14.0
95	9.5
96	5.0
97	4.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	981524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.4440512844848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88615638459856	21.375116701703266
2	9.499379841883771	5.214029351029186
3	3.436710578168915	2.829517840715974
4	1.5467275705518964	1.697938830774113
5	0.870126092215625	1.193989254936698
6	0.5654593579170756	0.9311097370780841
7	0.39095307843373117	0.7510535434053768
8	0.2919268219869408	0.6409323739141015
9	0.22613477427838452	0.5585448908251242
>10	3.5209614354235654	24.793324643999355
>50	1.517955126277523	29.294269348500606
>100	0.24190072969301696	8.731938736883283
>500	0.003738805713956984	0.7434973923320358
>1k	0.001869402856978492	1.2447373539028328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	4703	0.4791528276435421	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	2224	0.22658641052078196	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC	1871	0.19062193079333772	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGT	1745	0.17778475106059557	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1588	0.16178921758408352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0188237883128685E-4	0.0	0.0	0.6373761619685305	0.0
2	1.0188237883128685E-4	0.0	0.0	0.8280999751406996	0.0
3	1.0188237883128685E-4	0.0	0.0	1.087186864508662	0.0
4	1.0188237883128685E-4	0.0	0.0	2.3808893109083424	0.0
5	1.0188237883128685E-4	0.0	0.0	2.540233351400475	0.0
6	1.0188237883128685E-4	0.0	0.0	3.536744898749292	0.0
7	1.0188237883128685E-4	0.0	0.0	4.306771917956158	0.0
8	1.0188237883128685E-4	0.0	0.0	4.779098626218004	0.0
9	1.0188237883128685E-4	0.0	0.0	5.711322392524279	0.0
10	1.0188237883128685E-4	0.0	0.0	6.832639853941422	0.0
11	1.0188237883128685E-4	0.0	0.0	8.230058561991353	0.0
12	1.0188237883128685E-4	0.0	0.0	8.83625871603751	0.0
13	2.037647576625737E-4	0.0	0.0	9.0905571336004	0.0
14	2.037647576625737E-4	0.0	0.0	9.403641683748946	0.0
15	2.037647576625737E-4	0.0	0.0	9.620753033038417	0.0
16	2.037647576625737E-4	0.0	0.0	10.043157375672934	0.0
17	2.037647576625737E-4	0.0	0.0	10.527098675121545	0.0
18	2.037647576625737E-4	0.0	0.0	11.079708697902445	0.0
19	2.037647576625737E-4	0.0	0.0	11.390246188580209	0.0
20	2.037647576625737E-4	0.0	0.0	11.698032855029526	0.0
21	2.037647576625737E-4	0.0	0.0	12.077239069039575	0.0
22	2.037647576625737E-4	0.0	0.0	12.449822928425592	0.0
23	2.037647576625737E-4	0.0	0.0	12.823833141115244	0.0
24	2.037647576625737E-4	0.0	0.0	13.121431569681434	0.0
25	2.037647576625737E-4	0.0	0.0	13.36503233746704	0.0
26	2.037647576625737E-4	0.0	0.0	13.597833573096532	0.0
27	2.037647576625737E-4	0.0	0.0	13.826050101678614	0.0
28	2.037647576625737E-4	0.0	0.0	14.054062865503035	0.0
29	2.037647576625737E-4	0.0	0.0	14.294912809060197	0.0
30	2.037647576625737E-4	0.0	0.0	14.604635240707308	0.0
31	2.037647576625737E-4	0.0	0.0	14.8573035402089	0.0
32	2.037647576625737E-4	0.0	0.0	15.109156780679841	0.0
33	2.037647576625737E-4	0.0	0.0	15.372624612337548	0.0
34	2.037647576625737E-4	0.0	0.0	15.636499973510581	0.0
35	2.037647576625737E-4	0.0	0.0	15.905061924109853	0.0
36	2.037647576625737E-4	0.0	0.0	16.176170934179908	0.0
37	2.037647576625737E-4	0.0	0.0	16.43199758742527	0.0
38	3.056471364938606E-4	0.0	0.0	16.701476479434024	0.0
39	3.056471364938606E-4	0.0	0.0	16.985830198752144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.032782E-4	45.000004	31
CGAACTA	20	7.032782E-4	45.000004	13
ATCTACG	20	7.032782E-4	45.000004	1
CAACGAC	20	7.032782E-4	45.000004	10
ACGCAAC	20	7.032782E-4	45.000004	26
CGATTAT	20	7.032782E-4	45.000004	28
TAACGCA	20	7.032782E-4	45.000004	24
TCGATAG	20	7.032782E-4	45.000004	1
GTAATCG	20	7.032782E-4	45.000004	1
ATATACG	20	7.032782E-4	45.000004	1
ATGCGTC	20	7.032782E-4	45.000004	37
ATGCGAA	20	7.032782E-4	45.000004	12
CGCAACA	20	7.032782E-4	45.000004	27
CGATACG	20	7.032782E-4	45.000004	1
CTCGTTA	55	1.8189894E-12	45.0	14
CGTTATG	45	3.8380676E-10	45.0	1
GTTACGG	95	0.0	45.0	2
TCTCGAC	35	1.2116834E-7	45.0	24
GATATCG	25	3.8903887E-5	45.0	36
CGTACTA	25	3.8903887E-5	45.0	34
>>END_MODULE