FastQCFastQC Report
Sat 18 Jun 2016
SRR3552901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489934
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC65591.338751750235746No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT63211.290173778508942No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGT62351.2726203937673237No Hit
AATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT53951.1011687288491918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44020.8984883678209719No Hit
AAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT23250.47455371539840063No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT17940.366171770075153No Hit
AATGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT14390.293713030734752No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT11990.24472684075814294No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTAT10840.22125429139435107No Hit
AACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG10340.21104883514922418No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10230.2088036347752963No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC9520.19431188690721607No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT9100.18573930366130947No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8830.18022835728894096No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTCTGCTTC8560.17471741091657245No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.14512158780570444No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT5570.1136887825707136No Hit
ACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG5430.11083125482207808No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5150.10511619932480701No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACC207.0293184E-445.00000418
ACTATCG207.0293184E-445.0000041
CTCCGTA207.0293184E-445.00000443
GGTCGTT207.0293184E-445.00000426
TTGGACA207.0293184E-445.00000429
GGTAAGC207.0293184E-445.00000419
CAACGTT207.0293184E-445.00000434
CGGCCGT207.0293184E-445.00000441
TGGACGG207.0293184E-445.0000042
TAGGTAC207.0293184E-445.00000417
GGCAACG207.0293184E-445.0000041
CCTGTTA207.0293184E-445.00000423
TGTTGCG207.0293184E-445.00000412
CCACTAG207.0293184E-445.0000041
CGCTATG207.0293184E-445.0000041
CGCTAGT207.0293184E-445.00000422
GTCGTTG207.0293184E-445.00000427
GTACTGG750.045.0000042
CGTACAT302.162942E-645.00000435
TCGTACA302.162942E-645.00000434
Produced by FastQC (version 0.11.5)