##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489934
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.008084762437388	31.0	31.0	33.0	28.0	34.0
2	31.287148881277886	31.0	31.0	34.0	28.0	34.0
3	31.510468757016252	31.0	31.0	34.0	30.0	34.0
4	35.44929725228296	37.0	35.0	37.0	33.0	37.0
5	34.74712104079325	35.0	35.0	37.0	32.0	37.0
6	34.86855984683651	35.0	35.0	37.0	32.0	37.0
7	34.742530626574194	35.0	35.0	37.0	32.0	37.0
8	35.20594814811791	37.0	35.0	37.0	32.0	37.0
9	36.73227414304784	39.0	37.0	39.0	32.0	39.0
10	36.20397237178885	37.0	35.0	39.0	32.0	39.0
11	36.386460217090466	38.0	35.0	39.0	32.0	39.0
12	36.04210975355864	37.0	35.0	39.0	32.0	39.0
13	35.6412455555238	37.0	35.0	39.0	30.0	39.0
14	36.65744569676732	38.0	35.0	40.0	31.0	41.0
15	36.69674078549355	38.0	35.0	40.0	31.0	41.0
16	36.60661027811909	38.0	35.0	40.0	31.0	41.0
17	36.52536259986039	38.0	35.0	40.0	31.0	41.0
18	36.39975792657786	38.0	35.0	40.0	31.0	41.0
19	36.44026134132353	38.0	35.0	40.0	31.0	41.0
20	36.32169230957639	38.0	35.0	40.0	30.0	41.0
21	36.50033473896484	38.0	35.0	40.0	31.0	41.0
22	36.452667093935105	38.0	35.0	40.0	31.0	41.0
23	36.39377957030947	38.0	35.0	40.0	31.0	41.0
24	35.86281621606175	38.0	34.0	40.0	29.0	41.0
25	36.15705788943001	38.0	34.0	40.0	30.0	41.0
26	35.96983879461315	38.0	34.0	40.0	30.0	41.0
27	35.895216498548784	38.0	34.0	40.0	29.0	41.0
28	36.008915486575745	38.0	34.0	40.0	30.0	41.0
29	35.52353786428376	38.0	34.0	40.0	28.0	41.0
30	35.672325251972715	38.0	34.0	40.0	29.0	41.0
31	35.53176958529108	38.0	34.0	40.0	28.0	41.0
32	35.51946588724196	38.0	34.0	40.0	28.0	41.0
33	35.48204247919107	38.0	34.0	40.0	28.0	41.0
34	35.36719639788216	38.0	34.0	40.0	27.0	41.0
35	35.35813803491899	38.0	34.0	40.0	27.0	41.0
36	35.27293676291092	38.0	34.0	40.0	27.0	41.0
37	35.172088485387825	38.0	34.0	40.0	27.0	41.0
38	35.07721448195063	38.0	34.0	40.0	26.0	41.0
39	35.031663448546134	38.0	34.0	40.0	26.0	41.0
40	34.726673388660515	37.0	33.0	40.0	24.0	41.0
41	34.91528654880045	37.0	34.0	40.0	26.0	41.0
42	34.82205562381872	37.0	34.0	40.0	25.0	41.0
43	34.78848375495475	37.0	34.0	40.0	25.0	41.0
44	34.690666497936455	37.0	34.0	40.0	25.0	41.0
45	34.45163838394559	37.0	33.0	40.0	24.0	41.0
46	34.46133356737846	37.0	33.0	40.0	24.0	41.0
47	34.29626847697853	37.0	33.0	40.0	24.0	41.0
48	34.35318022427511	37.0	33.0	40.0	24.0	41.0
49	34.27943151526532	37.0	33.0	40.0	24.0	41.0
50	34.149563002363585	36.0	33.0	40.0	24.0	41.0
51	32.91161054346096	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	14.0
11	14.0
12	12.0
13	25.0
14	41.0
15	73.0
16	125.0
17	273.0
18	504.0
19	980.0
20	1672.0
21	2451.0
22	3182.0
23	3881.0
24	4263.0
25	4870.0
26	5271.0
27	6127.0
28	7334.0
29	9180.0
30	12294.0
31	16001.0
32	21511.0
33	30273.0
34	46884.0
35	50303.0
36	54904.0
37	73688.0
38	86213.0
39	47565.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.72680810068295	38.07410794106961	18.41982797682953	24.779255981417904
2	19.82777272040724	37.74630868647614	19.582025334024582	22.84389325909204
3	19.898394477623516	32.46784260737161	24.143456057346498	23.49030685765838
4	22.29198218535558	32.03594769907783	18.94500075520376	26.727069360362826
5	16.721435948515513	38.34557307718999	19.073793613017266	25.859197361277232
6	19.972894308212943	35.38027571060592	23.61052713222597	21.036302848955167
7	70.0888282911576	13.340368294504973	9.610069927786192	6.96073348655125
8	72.60896365632922	8.416235656231247	7.42406120008001	11.550739487359522
9	69.0758347042663	11.068633734339727	13.154628990843664	6.700902570550318
10	36.41919115635984	30.49288271481465	15.458000465368805	17.62992566345671
11	27.165291651528573	25.487922863079515	27.65433711479505	19.692448370596857
12	27.996015789881902	23.800552727510237	30.54207301391616	17.661358468691702
13	22.333212228585893	33.3040776920973	23.538068392885574	20.824641686431235
14	15.221642098731666	32.83013630407361	32.580102626068005	19.368118971126723
15	13.475284426065551	31.61364591965448	30.491658060065234	24.419411594214733
16	15.583119358934061	27.97417611351733	34.028052758126606	22.414651769422004
17	14.939155069866553	29.32537852037213	28.770405809762128	26.965060599999184
18	17.008821596378287	28.149097633558807	27.25836541248413	27.583715357578775
19	19.287699975915125	33.2156984410145	28.395049129066365	19.101552454004008
20	22.074197749084572	31.88449872840015	25.60467328252377	20.43663023999151
21	18.685169839202832	31.418313487122756	26.03493531781832	23.861581355856096
22	17.405405626063917	30.729241081451786	27.25999828548335	24.60535500700094
23	20.620532561528695	29.150252891205753	22.717957929027175	27.511256618238374
24	16.033179979344155	34.3411561557271	27.01363857172599	22.61202529320276
25	19.379549082121265	28.599362363093807	27.714141088391496	24.30694746639343
26	20.853829291292296	34.040095196495855	23.267419693264806	21.838655818947043
27	15.636595949658524	31.413823086374897	28.6312033865786	24.318377577387977
28	22.04256083472468	26.93975106851127	28.481795507149943	22.53589258961411
29	17.321516775728977	28.620589712083667	32.390077030783736	21.66781648140362
30	21.565557809827446	27.498397743369512	28.031938995864746	22.90410545093829
31	23.374168765588834	28.552009046116417	22.502622802254997	25.57119938603975
32	21.570456428825107	25.32953418215515	29.502545240787537	23.597464148232213
33	27.285716035221068	24.815587405650557	24.67658909159193	23.222107467536443
34	17.595023003098376	25.26605624431046	27.681279519282185	29.45764123330898
35	22.50221458400519	23.284973078006423	32.39783317753003	21.814979160458346
36	23.06167769536305	23.278645695134447	31.883886401025446	21.775790208477062
37	18.39064037196847	26.57643682618475	31.181751011360713	23.851171790486063
38	23.082905044352913	25.133793531373616	28.995129956279825	22.78817146799365
39	19.97820114546041	23.436830266933914	31.34707940253177	25.237889185073907
40	25.372805316634484	24.866614686876193	26.223532149228262	23.537047847261057
41	18.904383039348158	20.55011491343732	33.00689480623921	27.538607240975317
42	23.885053905219884	23.133115889078937	28.711826490915104	24.270003714786075
43	21.683736993146017	22.510583058126198	30.123445198741056	25.682234749986733
44	20.653598239762907	22.25667130674744	28.65222662644356	28.43750382704609
45	24.254287312168575	26.607053194920134	26.644609273902198	22.49405021900909
46	18.78232578265644	24.17305188045737	34.8722480987235	22.172374238162693
47	21.82579694407818	24.40328697334743	26.539084856327587	27.2318312262468
48	20.754224038339856	20.763613058085376	35.439263247702755	23.042899655872016
49	21.41390473002486	19.93942041172893	32.67378871439826	25.972886143847944
50	20.448876787485663	21.952344601517755	27.625353619058895	29.97342499193769
51	17.757902084770603	22.83756587622006	35.016349140904694	24.388182898104642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5188.0
1	4185.5
2	3183.0
3	2257.5
4	1332.0
5	1630.0
6	1928.0
7	1783.5
8	1639.0
9	1513.0
10	1387.0
11	1531.5
12	1676.0
13	1656.0
14	1636.0
15	1660.5
16	1685.0
17	1851.5
18	2018.0
19	1846.5
20	1675.0
21	1908.5
22	2142.0
23	2350.0
24	2558.0
25	2882.5
26	3763.0
27	4319.0
28	5291.0
29	6263.0
30	6866.5
31	7470.0
32	7776.5
33	8083.0
34	8860.0
35	9637.0
36	11252.5
37	12868.0
38	13801.0
39	14734.0
40	16940.5
41	19147.0
42	24249.0
43	29351.0
44	42125.5
45	54900.0
46	54523.0
47	54146.0
48	54782.0
49	55418.0
50	49154.0
51	42890.0
52	38674.0
53	34458.0
54	29356.5
55	24255.0
56	21447.0
57	18639.0
58	17214.5
59	15790.0
60	14091.5
61	12393.0
62	11159.5
63	9926.0
64	8271.0
65	6616.0
66	5669.0
67	4722.0
68	4048.0
69	3374.0
70	3146.5
71	2919.0
72	2799.5
73	2680.0
74	2100.0
75	1217.0
76	914.0
77	678.5
78	443.0
79	374.5
80	306.0
81	317.5
82	329.0
83	215.5
84	102.0
85	65.5
86	29.0
87	26.5
88	24.0
89	16.5
90	9.0
91	7.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	489934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.26074266681881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29568403344312	25.25876914525463
2	9.778128853938908	6.30899397439838
3	3.4557090448219823	3.3445122067920052
4	1.6068696399081488	2.0735523180880233
5	0.8665378262565377	1.3977576911963356
6	0.5622511511486772	1.088318382079807
7	0.41270411164224935	0.9319898800260067
8	0.3387705100657054	0.8743190598669338
9	0.23798123375773114	0.6909706207611196
>10	3.6186320020383094	29.554216526534965
>50	0.7497819600936966	15.273646837683428
>100	0.06423068530064746	4.02465015399997
>500	0.005087579033714651	1.2159913066604386
>1k	0.005087579033714651	2.933807269317407
>5k	0.0025437895168573255	5.028504627340535
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC	6559	1.338751750235746	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	6321	1.290173778508942	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGT	6235	1.2726203937673237	No Hit
AATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT	5395	1.1011687288491918	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4402	0.8984883678209719	No Hit
AAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT	2325	0.47455371539840063	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT	1794	0.366171770075153	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT	1439	0.293713030734752	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	1199	0.24472684075814294	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTAT	1084	0.22125429139435107	No Hit
AACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	1034	0.21104883514922418	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1023	0.2088036347752963	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	952	0.19431188690721607	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	910	0.18573930366130947	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	883	0.18022835728894096	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTCTGCTTC	856	0.17471741091657245	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	711	0.14512158780570444	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	557	0.1136887825707136	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	543	0.11083125482207808	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	515	0.10511619932480701	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.082182498050758E-4	0.0	0.0	1.526532145146081	0.0
2	4.082182498050758E-4	0.0	0.0	2.016802263161977	0.0
3	4.082182498050758E-4	0.0	0.0	2.7268979086979064	0.0
4	6.123273747076136E-4	0.0	0.0	6.468014059036523	0.0
5	6.123273747076136E-4	0.0	0.0	6.953793776304564	0.0
6	6.123273747076136E-4	0.0	0.0	9.341054101164646	0.0
7	6.123273747076136E-4	0.0	0.0	11.143541783178959	0.0
8	6.123273747076136E-4	0.0	0.0	11.930382459678242	0.0
9	6.123273747076136E-4	0.0	0.0	14.551755950801537	0.0
10	6.123273747076136E-4	0.0	0.0	16.309747843587097	0.0
11	6.123273747076136E-4	0.0	0.0	19.750415362069177	0.0
12	6.123273747076136E-4	0.0	0.0	20.77443084170521	0.0
13	6.123273747076136E-4	0.0	0.0	21.206733968248784	0.0
14	6.123273747076136E-4	0.0	0.0	21.851718802940805	0.0
15	6.123273747076136E-4	0.0	0.0	22.244220650128383	0.0
16	6.123273747076136E-4	0.0	0.0	22.97003269828181	0.0
17	6.123273747076136E-4	0.0	0.0	23.785856870517254	0.0
18	6.123273747076136E-4	0.0	0.0	25.055007409161234	0.0
19	6.123273747076136E-4	0.0	0.0	25.60304040952455	0.0
20	6.123273747076136E-4	0.0	0.0	26.011462768454525	0.0
21	6.123273747076136E-4	0.0	0.0	26.531124600456387	0.0
22	6.123273747076136E-4	0.0	0.0	27.05548094233101	0.0
23	6.123273747076136E-4	0.0	0.0	27.5696318279605	0.0
24	6.123273747076136E-4	0.0	0.0	27.951111782403345	0.0
25	6.123273747076136E-4	0.0	0.0	28.24911110476105	0.0
26	6.123273747076136E-4	0.0	0.0	28.54017071687207	0.0
27	6.123273747076136E-4	0.0	0.0	28.833679638481918	0.0
28	6.123273747076136E-4	0.0	0.0	29.139230998461016	0.0
29	6.123273747076136E-4	0.0	0.0	29.466622034804686	0.0
30	6.123273747076136E-4	0.0	0.0	29.874840284609764	0.0
31	6.123273747076136E-4	0.0	0.0	30.200394338829312	0.0
32	6.123273747076136E-4	0.0	0.0	30.497373115562503	0.0
33	6.123273747076136E-4	0.0	0.0	30.81945731465871	0.0
34	6.123273747076136E-4	0.0	0.0	31.095004633277135	0.0
35	6.123273747076136E-4	0.0	0.0	31.466075022349948	0.0
36	6.123273747076136E-4	0.0	0.0	31.768972963705316	0.0
37	6.123273747076136E-4	0.0	0.0	32.06472708568909	0.0
38	6.123273747076136E-4	0.0	0.0	32.3627264080468	0.0
39	6.123273747076136E-4	0.0	0.0	32.650112055909574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACC	20	7.0293184E-4	45.000004	18
ACTATCG	20	7.0293184E-4	45.000004	1
CTCCGTA	20	7.0293184E-4	45.000004	43
GGTCGTT	20	7.0293184E-4	45.000004	26
TTGGACA	20	7.0293184E-4	45.000004	29
GGTAAGC	20	7.0293184E-4	45.000004	19
CAACGTT	20	7.0293184E-4	45.000004	34
CGGCCGT	20	7.0293184E-4	45.000004	41
TGGACGG	20	7.0293184E-4	45.000004	2
TAGGTAC	20	7.0293184E-4	45.000004	17
GGCAACG	20	7.0293184E-4	45.000004	1
CCTGTTA	20	7.0293184E-4	45.000004	23
TGTTGCG	20	7.0293184E-4	45.000004	12
CCACTAG	20	7.0293184E-4	45.000004	1
CGCTATG	20	7.0293184E-4	45.000004	1
CGCTAGT	20	7.0293184E-4	45.000004	22
GTCGTTG	20	7.0293184E-4	45.000004	27
GTACTGG	75	0.0	45.000004	2
CGTACAT	30	2.162942E-6	45.000004	35
TCGTACA	30	2.162942E-6	45.000004	34
>>END_MODULE