FastQCFastQC Report
Sat 18 Jun 2016
SRR3552899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504381
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGCT57311.136244228073619No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45560.9032854132094587No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCT19700.39057775768714525No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTT15890.315039622824809No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCT15040.29818728302612507No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCC14370.28490367400833894No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGT11250.22304567380611087No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10520.2085724878613588No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8970.17784175058140572No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGC8330.16515293002710255No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCT7960.15781720564414597No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.14473185944752082No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCT5370.10646713496345024No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5260.1042862439306794No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGG253.887681E-545.0000042
CAGTTAG253.887681E-545.0000041
TTATCCG253.887681E-545.0000041
CCGTAGT502.1827873E-1145.00000427
CGTAGTG502.1827873E-1145.00000428
ATGGTCG253.887681E-545.00000444
CGTAATG253.887681E-545.0000041
GCAATAC253.887681E-545.00000434
GATTAGG850.045.0000042
TATCGCG253.887681E-545.0000041
TGCGTCT253.887681E-545.00000411
AGGTCGA253.887681E-545.00000410
CGGGTAT1100.045.06
ACGCATG453.8380676E-1045.01
GAGTACG351.2101191E-745.01
CGACATG351.2101191E-745.01
TCGATCG207.029517E-445.01
GACCCGT207.029517E-445.015
GCACCGT551.8189894E-1245.024
CTACGAA406.8012014E-945.010