##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504381
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046330056048898	31.0	31.0	33.0	28.0	34.0
2	31.32340234862138	31.0	31.0	34.0	28.0	34.0
3	31.543878536265243	31.0	31.0	34.0	30.0	34.0
4	35.51395671129563	37.0	35.0	37.0	33.0	37.0
5	34.85214748374741	35.0	35.0	37.0	32.0	37.0
6	34.95924311185394	35.0	35.0	37.0	32.0	37.0
7	34.91420771202722	36.0	35.0	37.0	32.0	37.0
8	35.35337373929629	37.0	35.0	37.0	33.0	37.0
9	36.79913597062538	39.0	37.0	39.0	32.0	39.0
10	36.26831898901822	38.0	35.0	39.0	32.0	39.0
11	36.463764495490516	38.0	35.0	39.0	32.0	39.0
12	36.19161308613925	37.0	35.0	39.0	32.0	39.0
13	35.81057573540637	37.0	35.0	39.0	30.0	39.0
14	36.836320162734125	39.0	35.0	40.0	31.0	41.0
15	36.84113398403191	38.0	35.0	40.0	32.0	41.0
16	36.76351805480381	38.0	35.0	40.0	31.0	41.0
17	36.67126239886118	38.0	35.0	40.0	31.0	41.0
18	36.59959435426791	38.0	35.0	40.0	31.0	41.0
19	36.63153449475694	38.0	35.0	40.0	31.0	41.0
20	36.48445123825045	38.0	35.0	40.0	31.0	41.0
21	36.53571803854626	38.0	35.0	40.0	31.0	41.0
22	36.528358522624764	38.0	35.0	40.0	31.0	41.0
23	36.45005263877902	38.0	35.0	40.0	31.0	41.0
24	36.00479201238746	38.0	34.0	40.0	30.0	41.0
25	36.234069086662664	38.0	35.0	40.0	30.0	41.0
26	36.12055569103515	38.0	35.0	40.0	30.0	41.0
27	36.063578921489906	38.0	34.0	40.0	30.0	41.0
28	36.08115690321404	38.0	35.0	40.0	30.0	41.0
29	35.644161853836685	38.0	34.0	40.0	29.0	41.0
30	35.72067147652271	38.0	34.0	40.0	29.0	41.0
31	35.552657613986256	38.0	34.0	40.0	28.0	41.0
32	35.56170632914404	38.0	34.0	40.0	29.0	41.0
33	35.45476732866623	38.0	34.0	40.0	28.0	41.0
34	35.30740848683832	38.0	34.0	40.0	27.0	41.0
35	35.30708928369625	38.0	34.0	40.0	27.0	41.0
36	35.1578092751313	38.0	34.0	40.0	26.0	41.0
37	35.06400716918361	38.0	34.0	40.0	26.0	41.0
38	34.965377363540654	38.0	34.0	40.0	26.0	41.0
39	34.920310241662555	37.0	34.0	40.0	25.0	41.0
40	34.67981149170964	37.0	33.0	40.0	25.0	41.0
41	34.7201064274824	37.0	34.0	40.0	25.0	41.0
42	34.66209274338248	37.0	33.0	40.0	25.0	41.0
43	34.65005224225337	37.0	33.0	40.0	25.0	41.0
44	34.59509180559934	37.0	33.0	40.0	25.0	41.0
45	34.439308776500305	37.0	33.0	40.0	24.0	41.0
46	34.39016735364734	37.0	33.0	40.0	24.0	41.0
47	34.284243458813876	37.0	33.0	40.0	24.0	41.0
48	34.275111473271195	37.0	33.0	40.0	24.0	41.0
49	34.159954478856264	36.0	33.0	40.0	24.0	41.0
50	34.07539142037468	36.0	33.0	40.0	24.0	41.0
51	32.92245544538751	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	12.0
11	13.0
12	17.0
13	18.0
14	39.0
15	60.0
16	128.0
17	261.0
18	550.0
19	1060.0
20	1778.0
21	2567.0
22	3443.0
23	4142.0
24	4698.0
25	4943.0
26	5460.0
27	6272.0
28	7475.0
29	9220.0
30	11620.0
31	15606.0
32	21287.0
33	30349.0
34	48106.0
35	51111.0
36	57228.0
37	76776.0
38	89540.0
39	50598.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.639320275743934	34.34546503536017	19.66687880788531	26.348335881010588
2	21.99210517446137	34.57862211304549	19.434316518663472	23.994956193829665
3	20.35227338063884	33.83196432855321	19.951187693430164	25.864574597377775
4	18.94658997860744	34.1898287207488	19.373449832567047	27.49013146807671
5	17.10076311359865	36.608040350449365	18.292520931597345	27.998675604354645
6	20.08679946310428	37.34775893620101	20.422458419329832	22.14298318136488
7	75.1067149634899	10.135591943392	8.07147771228496	6.68621538083314
8	77.02867475182451	9.135950799098302	6.875754637863044	6.959619811214142
9	70.82384150077024	11.821222448902715	11.55317111469306	5.801764935633975
10	36.68298369684821	32.06345996379721	13.762413730889941	17.49114260846463
11	28.79926087620271	27.26331880066854	25.767624077830053	18.16979624529869
12	29.101611678473216	23.691217551811032	27.55555819905984	19.651612570655914
13	23.24116094777559	31.30074289079089	24.95415172260652	20.503944438827
14	16.503198970619433	34.038554188202966	29.08575065278034	20.372496188397264
15	15.083835433927922	31.17563905063831	32.09062197029626	21.649903545137505
16	16.85630505510715	28.99117928708655	31.66217601376737	22.490339644038933
17	15.596344826629077	30.00727624553661	28.066679752012863	26.329699175821453
18	20.04615558476628	28.222117803803076	26.565037144539545	25.1666894668911
19	20.737894567796964	33.34324647439138	26.010892559394584	19.90796639841707
20	23.906927501234186	30.395276586548658	24.659136644718973	21.03865926749818
21	20.38974505383827	32.23297467588985	25.064187588350865	22.313092681921006
22	18.551650438854754	30.008862348105897	26.037856303072477	25.401630909966872
23	20.85586094638775	30.454953695718118	22.825403811801	25.863781546093133
24	18.101197309176992	31.343171134519345	26.790660235020745	23.764971321282918
25	18.9015843182039	29.303641493236267	26.589225208721185	25.205548979838653
26	20.137356482500333	32.66578241448429	23.424752320170665	23.772108782844715
27	17.1838352356651	30.581048849976504	25.392510820193465	26.842605094164927
28	18.20112177104213	29.086741966886144	30.526923099799557	22.18521316227217
29	18.88017193351851	28.781813747940543	27.496277615532705	24.84173670300824
30	19.627424506474274	27.607304795382852	27.015688537038468	25.7495821611044
31	22.123156899248784	29.87305231561062	23.709655994179005	24.294134790961593
32	23.671787795337256	26.406426887610756	24.009825905416736	25.911959411635248
33	20.79301163207972	26.562261465043292	26.059070424936703	26.58565647794029
34	17.411242691536756	28.47450637514101	28.685656279677467	25.428594653644765
35	20.738291093439283	25.062204960139262	27.69910048158039	26.50040346484106
36	20.81006223469956	27.32458201240729	27.764328949742357	24.101026803150795
37	19.950989430609003	29.077027088649253	26.395324169625738	24.576659311116
38	20.96867249162835	27.38128517925933	29.30721022401716	22.342832105095155
39	20.606644580188387	25.155388486084924	28.48679867005299	25.75116826367369
40	22.9580416391577	26.54778827909854	24.388507893834223	26.105662187909534
41	20.469644970766147	23.69300191720148	29.641084814852263	26.196268297180108
42	25.063989325529707	25.03424990235556	25.34096248669161	24.56079828542312
43	20.497401765728686	24.00784327720513	28.129132540678576	27.365622416387613
44	20.965896812132097	23.991387463048767	27.50995775019281	27.532757974626325
45	22.9417840878225	25.23647797993977	27.966557027326566	23.85518090491117
46	19.14326669719914	25.410156211276792	31.213903775122375	24.232673316401687
47	20.624686496914038	25.1540006463368	27.0577202551246	27.163592601624565
48	21.85351946246984	22.625554888070724	32.328140830047126	23.19278481941231
49	21.963951853856507	22.51670859925334	29.36351686522688	26.155822681663267
50	19.85027191745922	22.978462709737283	28.302414246373274	28.86885112643022
51	19.70752268622331	23.272882999161347	31.545994000567028	25.473600314048312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5334.0
1	4302.0
2	3270.0
3	2327.5
4	1385.0
5	1815.5
6	2246.0
7	2049.0
8	1852.0
9	1807.5
10	1763.0
11	1817.5
12	1872.0
13	1896.0
14	1920.0
15	2026.5
16	2133.0
17	2131.0
18	2129.0
19	2005.5
20	1882.0
21	1825.5
22	1769.0
23	2163.5
24	2558.0
25	2838.0
26	3489.0
27	3860.0
28	4677.0
29	5494.0
30	6326.5
31	7159.0
32	8312.5
33	9466.0
34	9773.5
35	10081.0
36	11707.0
37	13333.0
38	14123.5
39	14914.0
40	16801.0
41	18688.0
42	23604.5
43	28521.0
44	35320.5
45	42120.0
46	44828.0
47	47536.0
48	51255.0
49	54974.0
50	50639.5
51	46305.0
52	41370.0
53	36435.0
54	32297.0
55	28159.0
56	25229.0
57	22299.0
58	19805.5
59	17312.0
60	16090.0
61	14868.0
62	13806.0
63	12744.0
64	11537.0
65	10330.0
66	9157.0
67	7984.0
68	6919.5
69	5855.0
70	4955.5
71	4056.0
72	3777.0
73	3498.0
74	2752.5
75	1611.0
76	1215.0
77	1118.5
78	1022.0
79	799.5
80	577.0
81	408.0
82	239.0
83	148.0
84	57.0
85	37.0
86	17.0
87	15.5
88	14.0
89	10.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.775644306057764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.28077014707134	26.439834484917462
2	9.771784419672583	6.6009662958867645
3	3.403527552119704	3.448690079587878
4	1.5883647003468293	2.145921645888501
5	0.9390748116275065	1.585892840715443
6	0.6306985747843455	1.2781350435752177
7	0.4635266570850523	1.0959138047256516
8	0.35181355930049013	0.9506183712785211
9	0.2536746877126498	0.7711223419469466
>10	3.378610319858394	27.499423059698035
>50	0.8584498083022477	19.38775972219834
>100	0.07143908308429986	4.152782195906159
>500	0.003542433871948753	0.861266089033311
>1k	0.004132839517273545	2.638836340860802
>5k	5.904056453247922E-4	1.1428376837809457
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGCT	5731	1.136244228073619	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4556	0.9032854132094587	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCT	1970	0.39057775768714525	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTT	1589	0.315039622824809	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCT	1504	0.29818728302612507	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCC	1437	0.28490367400833894	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGT	1125	0.22304567380611087	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1052	0.2085724878613588	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	897	0.17784175058140572	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGC	833	0.16515293002710255	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCT	796	0.15781720564414597	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	730	0.14473185944752082	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCT	537	0.10646713496345024	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	526	0.1042862439306794	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9652564232197485E-4	0.0	0.0	1.3471958697889095	0.0
2	3.9652564232197485E-4	0.0	0.0	1.782382762237277	0.0
3	3.9652564232197485E-4	0.0	0.0	2.2788328664243895	0.0
4	3.9652564232197485E-4	0.0	0.0	4.551519585392788	0.0
5	3.9652564232197485E-4	0.0	0.0	4.956372266203525	0.0
6	3.9652564232197485E-4	0.0	0.0	7.103360356555857	0.0
7	3.9652564232197485E-4	0.0	0.0	8.752113977330628	0.0
8	3.9652564232197485E-4	0.0	0.0	9.866152769434217	0.0
9	3.9652564232197485E-4	0.0	0.0	11.423110704011451	0.0
10	3.9652564232197485E-4	0.0	0.0	13.488017986403136	0.0
11	3.9652564232197485E-4	0.0	0.0	15.807098205523205	0.0
12	3.9652564232197485E-4	0.0	0.0	16.961582613143637	0.0
13	3.9652564232197485E-4	0.0	0.0	17.461799710932805	0.0
14	3.9652564232197485E-4	0.0	0.0	17.955474135623664	0.0
15	3.9652564232197485E-4	0.0	0.0	18.289150463637608	0.0
16	3.9652564232197485E-4	0.0	0.0	19.003887933922968	0.0
17	3.9652564232197485E-4	0.0	0.0	19.809033250657738	0.0
18	3.9652564232197485E-4	0.0	0.0	20.941510485129296	0.0
19	5.947884634829623E-4	0.0	0.0	21.431616179039256	0.0
20	5.947884634829623E-4	0.0	0.0	21.882267571538183	0.0
21	5.947884634829623E-4	0.0	0.0	22.392199547564243	0.0
22	5.947884634829623E-4	0.0	0.0	22.884684395328136	0.0
23	5.947884634829623E-4	0.0	0.0	23.397788576492772	0.0
24	5.947884634829623E-4	0.0	0.0	23.77072094309659	0.0
25	5.947884634829623E-4	0.0	0.0	24.09230323901971	0.0
26	5.947884634829623E-4	0.0	0.0	24.400601925925045	0.0
27	5.947884634829623E-4	0.0	0.0	24.727933843661834	0.0
28	5.947884634829623E-4	0.0	0.0	25.05249008190237	0.0
29	5.947884634829623E-4	0.0	0.0	25.38755424966444	0.0
30	5.947884634829623E-4	0.0	0.0	25.834438648561306	0.0
31	5.947884634829623E-4	0.0	0.0	26.16296014322506	0.0
32	5.947884634829623E-4	0.0	0.0	26.4478638172334	0.0
33	5.947884634829623E-4	0.0	0.0	26.763498228521694	0.0
34	5.947884634829623E-4	0.0	0.0	27.057125466661116	0.0
35	5.947884634829623E-4	0.0	0.0	27.392387897244344	0.0
36	5.947884634829623E-4	0.0	0.0	27.680067250748937	0.0
37	5.947884634829623E-4	0.0	0.0	27.960807405512895	0.0
38	5.947884634829623E-4	0.0	0.0	28.263158207783402	0.0
39	5.947884634829623E-4	0.0	0.0	28.58236134985259	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGG	25	3.887681E-5	45.000004	2
CAGTTAG	25	3.887681E-5	45.000004	1
TTATCCG	25	3.887681E-5	45.000004	1
CCGTAGT	50	2.1827873E-11	45.000004	27
CGTAGTG	50	2.1827873E-11	45.000004	28
ATGGTCG	25	3.887681E-5	45.000004	44
CGTAATG	25	3.887681E-5	45.000004	1
GCAATAC	25	3.887681E-5	45.000004	34
GATTAGG	85	0.0	45.000004	2
TATCGCG	25	3.887681E-5	45.000004	1
TGCGTCT	25	3.887681E-5	45.000004	11
AGGTCGA	25	3.887681E-5	45.000004	10
CGGGTAT	110	0.0	45.0	6
ACGCATG	45	3.8380676E-10	45.0	1
GAGTACG	35	1.2101191E-7	45.0	1
CGACATG	35	1.2101191E-7	45.0	1
TCGATCG	20	7.029517E-4	45.0	1
GACCCGT	20	7.029517E-4	45.0	15
GCACCGT	55	1.8189894E-12	45.0	24
CTACGAA	40	6.8012014E-9	45.0	10
>>END_MODULE