FastQCFastQC Report
Sat 18 Jun 2016
SRR3552896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357110
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT57491.6098681078659236TruSeq Adapter, Index 1 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40461.1329842345495786No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC32630.9137240626137605No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGT30550.8554787040407719No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT28080.7863123407353477Illumina Paired End PCR Primer 2 (95% over 22bp)
AAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT18480.5174876088600151RNA PCR Primer, Index 33 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT13840.38755565512027107No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10810.30270784912211923No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC8680.2430623617372798Illumina Paired End PCR Primer 2 (95% over 24bp)
AAAAAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCT8050.22542073870796112No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7960.2229005068466299No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT7490.2097392960152334No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCT6470.18117666825347933No Hit
AACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG6460.18089664249110918Illumina Paired End PCR Primer 2 (95% over 23bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.15737447845201757No Hit
AACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT5280.1478536025314329Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTAT5210.14589342219484194No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG5150.1442132676206211TruSeq Adapter, Index 1 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5030.14085295847217943No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCT4600.12881185069026352No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCGATTTC4310.12069110358152949No Hit
TACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT4290.12013105205678923Illumina Paired End PCR Primer 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC3960.11089020189857467Illumina Paired End PCR Primer 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC3910.10949007308672398Illumina Paired End PCR Primer 2 (95% over 24bp)
AGCCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT3880.10864999579961357RNA PCR Primer, Index 1 (95% over 21bp)
GAAAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT3750.10500966088880176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAACG302.1612832E-645.0000041
CCGGGTA302.1612832E-645.0000045
ATGATCG502.1827873E-1145.00000427
CTCCGCG253.885386E-545.0000041
TACCACG253.885386E-545.0000041
CTATCGG302.1612832E-645.0000042
TTTCGGG302.1612832E-645.0000043
CGTGAGT253.885386E-545.00000432
TTGTCGC302.1612832E-645.00000438
CGCGAGG502.1827873E-1145.0000042
CGACACG253.885386E-545.0000041
TAGCACG253.885386E-545.0000041
GCTCTAG302.1612832E-645.0000041
TACGATT253.885386E-545.00000411
TATTCGT253.885386E-545.00000415
CGGGCAC253.885386E-545.0000046
CGCAGTG302.1612832E-645.00000442
GACGGGT302.1612832E-645.0000044
TATAGCG302.1612832E-645.0000041
CGCGTGG253.885386E-545.0000042
Produced by FastQC (version 0.11.5)