##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552895_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305334
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93324032043598	31.0	31.0	33.0	28.0	34.0
2	31.216490793688223	31.0	31.0	34.0	28.0	34.0
3	31.440376767736314	31.0	31.0	34.0	30.0	34.0
4	35.41109408058061	37.0	35.0	37.0	33.0	37.0
5	34.68734238571532	35.0	35.0	37.0	32.0	37.0
6	34.78771443730472	35.0	35.0	37.0	32.0	37.0
7	34.600152619754105	35.0	35.0	37.0	31.0	37.0
8	35.11140587029286	36.0	35.0	37.0	32.0	37.0
9	36.6030347095312	38.0	35.0	39.0	32.0	39.0
10	36.106833827873736	37.0	35.0	39.0	31.0	39.0
11	36.289067054438746	37.0	35.0	39.0	32.0	39.0
12	35.77674284553964	37.0	35.0	39.0	31.0	39.0
13	35.20151375215338	37.0	34.0	39.0	30.0	39.0
14	36.17597450660588	38.0	35.0	40.0	30.0	41.0
15	36.34844465405097	38.0	35.0	40.0	31.0	41.0
16	36.271738489653956	38.0	35.0	40.0	31.0	41.0
17	36.18234458003367	38.0	35.0	40.0	31.0	41.0
18	36.044407108281426	38.0	35.0	40.0	30.0	41.0
19	36.01393228399065	38.0	34.0	40.0	30.0	41.0
20	35.91118905853917	37.0	34.0	40.0	30.0	41.0
21	36.02360366025402	38.0	34.0	40.0	30.0	41.0
22	35.96855902061349	38.0	34.0	40.0	30.0	41.0
23	35.87426228327013	38.0	34.0	40.0	30.0	41.0
24	35.3816083370997	37.0	34.0	40.0	27.0	41.0
25	35.629173298748256	37.0	34.0	40.0	29.0	41.0
26	35.49927620245371	37.0	34.0	40.0	29.0	41.0
27	35.356167344612786	37.0	34.0	40.0	27.0	41.0
28	35.4552424558025	37.0	34.0	40.0	29.0	41.0
29	35.01259604236672	37.0	34.0	40.0	27.0	41.0
30	35.05512979229303	37.0	34.0	40.0	27.0	41.0
31	34.909007840594235	37.0	34.0	40.0	26.0	41.0
32	34.74781386940203	37.0	33.0	40.0	25.0	41.0
33	34.761559472577574	37.0	33.0	40.0	25.0	41.0
34	34.70251920847334	37.0	34.0	40.0	25.0	41.0
35	34.502286021209564	37.0	33.0	40.0	24.0	41.0
36	34.57310355217565	37.0	33.0	40.0	25.0	41.0
37	34.36244899028605	37.0	33.0	40.0	24.0	41.0
38	34.23965559027164	37.0	33.0	40.0	24.0	41.0
39	34.23808681640433	37.0	33.0	40.0	23.0	41.0
40	33.91965847236141	36.0	33.0	40.0	23.0	41.0
41	34.050976962932396	36.0	33.0	40.0	23.0	41.0
42	33.91804384706584	36.0	33.0	40.0	23.0	41.0
43	33.94263003792568	36.0	33.0	40.0	23.0	41.0
44	33.82229296442584	36.0	33.0	39.0	23.0	41.0
45	33.62249864083266	35.0	33.0	39.0	23.0	40.0
46	33.61680978862491	35.0	33.0	39.0	23.0	40.0
47	33.500835151014954	35.0	33.0	39.0	22.0	40.0
48	33.547495529485744	35.0	33.0	39.0	22.0	40.0
49	33.41184407894306	35.0	33.0	39.0	22.0	40.0
50	33.32157571708359	35.0	32.0	39.0	21.0	40.0
51	32.07074875382368	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	1.0
11	10.0
12	11.0
13	11.0
14	26.0
15	54.0
16	92.0
17	195.0
18	406.0
19	812.0
20	1320.0
21	2111.0
22	2736.0
23	3071.0
24	3420.0
25	3568.0
26	3905.0
27	4451.0
28	5255.0
29	6668.0
30	8528.0
31	11237.0
32	15200.0
33	21464.0
34	33883.0
35	34203.0
36	33108.0
37	41854.0
38	44949.0
39	22779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.77714240798601	37.870004650644866	18.08937098390615	25.263481957462975
2	20.278449173691758	38.77589786921863	19.041770651155783	21.90388230593383
3	19.717751707965704	33.14370492640846	23.39012360235021	23.748419763275628
4	21.811524428985962	33.21575717083587	19.05192346741601	25.920794932762153
5	16.001493446520858	39.11945607105661	19.46294877085421	25.416101711568317
6	19.12004558942011	37.13408922687942	23.433682459208605	20.31218272449187
7	67.58598780352007	15.284246104266147	9.879017731402334	7.250748360811439
8	70.11141897070094	10.58414719618516	7.8409217447123485	11.463512088401554
9	67.29777882581043	12.653684162261655	13.31525477018609	6.733282241741831
10	39.81934537260835	28.262165366451164	14.33970668186314	17.578782579077338
11	31.887703301957853	26.29579411398665	23.746782212265913	18.06972037178958
12	30.67329547315399	24.235427433564556	28.438365855096386	16.65291123818507
13	22.023423529642947	36.79020351483949	21.862288510287094	19.32408444523047
14	14.187741948161687	37.57000530566527	30.003537110180982	18.23871563599206
15	12.909797140180917	31.696764854225208	33.15811537529394	22.235322630299933
16	14.934465208591247	28.522208466793742	33.88387798279916	22.659448341815846
17	13.333595341494888	29.17984895229486	28.03520079650481	29.451354909705437
18	17.635769354215384	29.331486175794375	25.804856321274407	27.227888148715834
19	19.90050240064978	34.02470736963457	27.071993292591063	19.002796937124593
20	23.745144661256198	32.238794238440526	23.475603765057283	20.540457335245993
21	17.78642404710907	33.0759103146063	25.249726528981377	23.887939109303254
22	17.466774090012905	31.822528771771243	25.07745616275947	25.633240975456385
23	19.868078890657443	31.85593481236941	21.00028165877367	27.27570463819948
24	15.848873692415518	33.70309235132674	26.855509049106878	23.592524907150857
25	16.932604950644212	28.883452219536636	26.86598937556905	27.317953454250098
26	20.656395946733742	35.10221593402634	21.594057654895952	22.647330464343966
27	15.334682675365338	32.947198805242785	25.030294693679707	26.68782382571217
28	18.386095226866317	27.918607164613178	29.031486830814778	24.663810777705724
29	19.82976019703014	30.11489057884153	28.090877530835083	21.964471693293248
30	18.382492614644946	29.641310826832257	25.147215835773284	26.828980722749513
31	25.25136407999109	29.777882581042398	22.1914362632396	22.779317075726908
32	23.69274302894535	27.965113613289056	23.281390215305205	25.060753142460392
33	22.403007853694643	27.13291019015242	25.919812402156328	24.544269553996607
34	18.699850000327512	26.712714601059822	24.94481453097264	29.64262086764003
35	18.40771090019454	28.835963240254937	26.95212455868	25.80420130087052
36	23.960973884336497	24.26752343335495	30.102445191167703	21.66905749114085
37	18.867207713520276	30.039890742596633	25.791428402994754	25.301473140888337
38	20.237837908650853	28.11052814295165	30.299606332737266	21.352027615660226
39	19.283473180189564	25.43673485429071	31.331263468857056	23.94852849666267
40	22.565780424060208	27.372320147772605	24.746998368999197	25.314901059167994
41	17.982275147870855	23.231936174811846	30.544583963790473	28.241204713526823
42	24.74274073637394	26.16282497199788	23.180517073106827	25.91391721852136
43	21.999842795103067	23.733026783784315	28.14491671415564	26.12221370695697
44	21.02255235250578	23.572546784832348	26.771338927207584	28.63356193545429
45	23.63051609057622	28.073191979930172	26.227016971578664	22.06927495791494
46	18.855089836048393	24.549837227429634	33.722087943039426	22.872984993482547
47	20.349191377311403	25.889681463577592	26.589243254927393	27.171883904183613
48	21.687398062449645	20.509016355859487	34.872303772262505	22.931281809428363
49	21.799079041312137	21.00028165877367	30.908775308350855	26.291863991563336
50	19.36174811845389	23.220145807541908	27.44568243300779	29.972423640996414
51	18.181401350652074	23.681280171877354	32.793596520531615	25.343721956938957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4310.0
1	3504.0
2	2698.0
3	1884.5
4	1071.0
5	1161.5
6	1252.0
7	1261.0
8	1270.0
9	1244.5
10	1219.0
11	1218.0
12	1217.0
13	1281.5
14	1346.0
15	1356.5
16	1367.0
17	1574.0
18	1781.0
19	1664.0
20	1547.0
21	1532.5
22	1518.0
23	1711.5
24	1905.0
25	1911.0
26	2092.5
27	2268.0
28	2530.0
29	2792.0
30	3051.5
31	3311.0
32	3767.0
33	4223.0
34	4632.0
35	5041.0
36	5268.5
37	5496.0
38	6272.0
39	7048.0
40	8431.5
41	9815.0
42	13764.5
43	17714.0
44	26054.0
45	34394.0
46	34500.0
47	34606.0
48	35326.5
49	36047.0
50	31810.5
51	27574.0
52	25162.0
53	22750.0
54	19713.5
55	16677.0
56	14282.5
57	11888.0
58	10758.5
59	9629.0
60	8538.5
61	7448.0
62	6489.5
63	5531.0
64	4868.0
65	4205.0
66	3771.0
67	3337.0
68	3219.0
69	3101.0
70	2562.0
71	2023.0
72	1687.5
73	1352.0
74	1273.5
75	919.0
76	643.0
77	483.0
78	323.0
79	338.0
80	353.0
81	217.5
82	82.0
83	57.5
84	33.0
85	20.0
86	7.0
87	5.0
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	305334.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.16302745857033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.11182147961061	26.68536302046881
2	9.985568141092731	6.822744771871522
3	3.353553669486654	3.437026382833856
4	1.5686327287915431	2.1435697194447036
5	0.9423337526122783	1.6096486932815446
6	0.5969241967948824	1.2235642635473165
7	0.4531812310863251	1.0837429988917582
8	0.339386626152412	0.9275579702653117
9	0.2994587647322421	0.9207376202031428
>10	3.6643770422548774	29.149870690121453
>50	0.6108958729444455	13.273626189522805
>100	0.059891752249455435	3.8384527897767104
>500	0.007985566966594058	2.0948485685499585
>1k	0.005989175224945543	6.789246321221085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT	4400	1.4410448885482783	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3741	1.2252156654679793	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC	3632	1.1895170534562152	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGT	3461	1.133512808923998	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT	3387	1.1092770539802315	No Hit
AAACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT	1288	0.4218331401023142	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	948	0.3104796714417654	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTT	870	0.28493387569022777	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTT	834	0.2731435084202873	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCT	759	0.24858024327457803	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	743	0.24334008004349336	No Hit
AACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	706	0.2312222025716101	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCTTTCATC	676	0.2213968965133264	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	607	0.19879869257927388	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCT	489	0.16015248875002455	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTAT	488	0.15982497854808178	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	395	0.12936652976740226	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.1205237543149469	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	333	0.10906089724694924	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	317	0.1038207340158646	No Hit
AATGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	314	0.10283820341003623	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCT	312	0.10218318300615065	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	309	0.10120065240032228	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.825306058283715E-4	0.0	0.0	1.6974853766694833	0.0
2	9.825306058283715E-4	0.0	0.0	2.2303444752304036	0.0
3	9.825306058283715E-4	0.0	0.0	2.9934432457571054	0.0
4	9.825306058283715E-4	0.0	0.0	6.660247466708588	0.0
5	9.825306058283715E-4	0.0	0.0	7.163630647094657	0.0
6	9.825306058283715E-4	0.0	0.0	9.505983611389494	0.0
7	9.825306058283715E-4	0.0	0.0	11.370171680847859	0.0
8	9.825306058283715E-4	0.0	0.0	12.456523020692094	0.0
9	9.825306058283715E-4	0.0	0.0	15.057609044521737	0.0
10	9.825306058283715E-4	0.0	0.0	17.159569520590566	0.0
11	9.825306058283715E-4	0.0	0.0	20.509016355859487	0.0
12	9.825306058283715E-4	0.0	0.0	21.737834633548836	0.0
13	9.825306058283715E-4	0.0	0.0	22.255300752618442	0.0
14	9.825306058283715E-4	0.0	0.0	22.901805891253513	0.0
15	9.825306058283715E-4	0.0	0.0	23.306936011056745	0.0
16	9.825306058283715E-4	0.0	0.0	24.030078536946426	0.0
17	9.825306058283715E-4	0.0	0.0	24.85081910301506	0.0
18	9.825306058283715E-4	0.0	0.0	26.340990521854756	0.0
19	9.825306058283715E-4	0.0	0.0	26.859766681732136	0.0
20	9.825306058283715E-4	0.0	0.0	27.321883576673414	0.0
21	9.825306058283715E-4	0.0	0.0	27.83672961412748	0.0
22	9.825306058283715E-4	0.0	0.0	28.319807161993097	0.0
23	9.825306058283715E-4	0.0	0.0	28.78487164875186	0.0
24	9.825306058283715E-4	0.0	0.0	29.128102340387905	0.0
25	9.825306058283715E-4	0.0	0.0	29.428756705771384	0.0
26	9.825306058283715E-4	0.0	0.0	29.7005901733839	0.0
27	9.825306058283715E-4	0.0	0.0	29.98585155927607	0.0
28	9.825306058283715E-4	0.0	0.0	30.265545271735213	0.0
29	9.825306058283715E-4	0.0	0.0	30.573077351359494	0.0
30	9.825306058283715E-4	0.0	0.0	31.005063307722036	0.0
31	9.825306058283715E-4	0.0	0.0	31.28966967321032	0.0
32	9.825306058283715E-4	0.0	0.0	31.55462542658204	0.0
33	9.825306058283715E-4	0.0	0.0	31.83300909823341	0.0
34	9.825306058283715E-4	0.0	0.0	32.103532525038155	0.0
35	9.825306058283715E-4	0.0	0.0	32.43366280859649	0.0
36	9.825306058283715E-4	0.0	0.0	32.66422999076421	0.0
37	0.001310040807771162	0.0	0.0	32.899709825961075	0.0
38	0.001310040807771162	0.0	0.0	33.169578232361935	0.0
39	0.001310040807771162	0.0	0.0	33.46368239370656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAGC	20	7.025139E-4	45.000004	4
ATGGGAC	80	0.0	45.000004	5
AGGTAAT	20	7.025139E-4	45.000004	6
ATTAGTT	20	7.025139E-4	45.000004	29
CAAGTCT	20	7.025139E-4	45.000004	39
CGAAATC	20	7.025139E-4	45.000004	22
GGATGTT	20	7.025139E-4	45.000004	8
ACGCATG	20	7.025139E-4	45.000004	1
TAGACGA	20	7.025139E-4	45.000004	17
TTTCGCG	20	7.025139E-4	45.000004	1
TGGCGAT	20	7.025139E-4	45.000004	29
ACCGAAC	20	7.025139E-4	45.000004	10
CAATTCG	20	7.025139E-4	45.000004	41
ACGATCT	20	7.025139E-4	45.000004	20
CTAACGG	20	7.025139E-4	45.000004	2
CTAGTCA	20	7.025139E-4	45.000004	31
CGTAGTG	20	7.025139E-4	45.000004	28
TTCCACG	20	7.025139E-4	45.000004	1
GTGTCTA	20	7.025139E-4	45.000004	34
TCAGACA	20	7.025139E-4	45.000004	24
>>END_MODULE