SRR3552893_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325532
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	5040	1.5482348893503557	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3501	1.0754703070665865	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC	1755	0.5391175061130703	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1645	0.5053266652740744	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGT	1611	0.49488222356020295	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1230	0.37784303847240824	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	828	0.25435287467898704	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	827	0.2540456852168143	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	801	0.24605875920032438	Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	647	0.19875158202573018	No Hit
AACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	569	0.1747908039762604	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	420	0.12901957411252965	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	382	0.11734637454996744	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	380	0.11673199562562207	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGTAGGTC	380	0.11673199562562207	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	365	0.1121241536930317	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	359	0.11028101691999558	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	356	0.1093594485334775	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	344	0.10567317498740524	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	344	0.10567317498740524	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	328	0.1007581435926422	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATTC	50	2.1827873E-11	45.0	42
CTTCGTC	20	7.0258294E-4	45.0	37
CGGGTTC	40	6.7884685E-9	45.0	6
ATCCTAA	20	7.0258294E-4	45.0	25
TCGTTGA	55	1.8189894E-12	45.0	24
ACACGTG	20	7.0258294E-4	45.0	42
TCACCGG	25	3.884625E-5	45.0	2
CTACTGA	20	7.0258294E-4	45.0	6
ATCTAGT	20	7.0258294E-4	45.0	10
GCGAATC	25	3.884625E-5	45.0	40
CTATCGC	20	7.0258294E-4	45.0	11
CATTGTC	20	7.0258294E-4	45.0	37
TTTCGAA	20	7.0258294E-4	45.0	31
GGCAATT	20	7.0258294E-4	45.0	8
TGGCGTG	20	7.0258294E-4	45.0	25
CGCATCG	35	1.2083365E-7	45.0	21
AATGGTC	85	0.0	45.0	9
TTACTAA	20	7.0258294E-4	45.0	17
GATCAAT	20	7.0258294E-4	45.0	9
ATAGACG	25	3.884625E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content