##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325532
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.152599437228904	31.0	31.0	33.0	28.0	34.0
2	31.41749198235504	31.0	31.0	34.0	30.0	34.0
3	31.58520821301746	31.0	31.0	34.0	30.0	34.0
4	35.5259728690267	37.0	35.0	37.0	33.0	37.0
5	34.87232898762641	35.0	35.0	37.0	32.0	37.0
6	34.95525171104531	35.0	35.0	37.0	32.0	37.0
7	34.86313173512896	35.0	35.0	37.0	32.0	37.0
8	35.29336900826954	37.0	35.0	37.0	33.0	37.0
9	36.74162908715579	39.0	37.0	39.0	32.0	39.0
10	36.293875870882125	38.0	35.0	39.0	32.0	39.0
11	36.49402209306612	38.0	35.0	39.0	32.0	39.0
12	36.15953270339014	37.0	35.0	39.0	32.0	39.0
13	35.73928830345404	37.0	35.0	39.0	30.0	39.0
14	36.8168044923387	39.0	35.0	40.0	31.0	41.0
15	36.82335991546146	38.0	35.0	40.0	32.0	41.0
16	36.73139967806544	38.0	35.0	40.0	31.0	41.0
17	36.61584114618532	38.0	35.0	40.0	31.0	41.0
18	36.52530626789379	38.0	35.0	40.0	31.0	41.0
19	36.562571421549954	38.0	35.0	40.0	31.0	41.0
20	36.420579236449875	38.0	35.0	40.0	31.0	41.0
21	36.54578658933684	38.0	35.0	40.0	31.0	41.0
22	36.498424118059056	38.0	35.0	40.0	31.0	41.0
23	36.442478773208165	38.0	35.0	40.0	31.0	41.0
24	36.00207967265891	38.0	34.0	40.0	30.0	41.0
25	36.19210707395894	38.0	35.0	40.0	30.0	41.0
26	36.103396901072706	38.0	35.0	40.0	30.0	41.0
27	36.006051632404805	38.0	34.0	40.0	30.0	41.0
28	36.02911848911935	38.0	35.0	40.0	30.0	41.0
29	35.533735546735805	38.0	34.0	40.0	27.0	41.0
30	35.58555533710972	38.0	34.0	40.0	29.0	41.0
31	35.53468476217392	38.0	34.0	40.0	28.0	41.0
32	35.443034786134696	38.0	34.0	40.0	28.0	41.0
33	35.31176044136982	38.0	34.0	40.0	27.0	41.0
34	35.33312546846393	38.0	34.0	40.0	27.0	41.0
35	35.202781907769435	38.0	34.0	40.0	27.0	41.0
36	35.032906135187936	38.0	34.0	40.0	26.0	41.0
37	34.92258825553248	37.0	34.0	40.0	26.0	41.0
38	34.78176031849404	37.0	34.0	40.0	25.0	41.0
39	34.749287320447756	37.0	33.0	40.0	25.0	41.0
40	34.5577424032046	37.0	33.0	40.0	24.0	41.0
41	34.66002420652962	37.0	33.0	40.0	25.0	41.0
42	34.58980376737156	37.0	33.0	40.0	25.0	41.0
43	34.49314660309893	37.0	33.0	40.0	24.0	41.0
44	34.40603688731062	37.0	33.0	40.0	24.0	41.0
45	34.27880208397331	37.0	33.0	40.0	23.0	41.0
46	34.24853163437081	37.0	33.0	40.0	23.0	41.0
47	34.150089699322955	36.0	33.0	40.0	23.0	41.0
48	34.153302901097284	36.0	33.0	40.0	23.0	41.0
49	34.0046232014057	36.0	33.0	40.0	23.0	41.0
50	33.8814617303368	36.0	33.0	39.0	23.0	41.0
51	32.6409200938771	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	3.0
10	7.0
11	9.0
12	10.0
13	16.0
14	31.0
15	36.0
16	89.0
17	180.0
18	322.0
19	672.0
20	1226.0
21	1722.0
22	2290.0
23	2701.0
24	3150.0
25	3381.0
26	3598.0
27	4076.0
28	4826.0
29	6057.0
30	7694.0
31	10276.0
32	13807.0
33	19781.0
34	31899.0
35	33719.0
36	36766.0
37	49576.0
38	57219.0
39	30387.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.176609365592324	34.50474914908519	19.730779155351854	26.587862329970633
2	22.042687047663517	35.44597766118231	20.080360763304377	22.4309745278498
3	20.237641767936793	34.00587346251674	20.988412813486846	24.76807195605962
4	20.84464814519003	34.02983424056621	20.24962215696153	24.875895457282233
5	16.768243982158438	37.98428418711525	18.170563876976765	27.076907953749558
6	19.35477925365248	36.881781207377465	22.24881117678139	21.514628362188663
7	71.93179165181918	12.203408574272267	8.846134942186943	7.018664831721614
8	74.27257535357506	9.375422385510488	7.606011083395796	8.745991177518645
9	68.85836108278141	12.428271260582676	12.519506530847964	6.193861125787941
10	36.90973544843518	31.67430544462603	14.054225083862724	17.36173402307607
11	29.859737291571946	27.614796702013933	23.96876497548628	18.556701030927837
12	29.91595296314955	24.08303945541452	28.577835665925317	17.42317191551061
13	23.729464384453756	32.061056977501444	23.20539916198715	21.00407947605765
14	16.517270191563348	33.84306304756522	30.28795940184068	19.351707359030755
15	15.693388054016195	30.45261295356524	31.664782571298673	22.18921642111989
16	16.57532899991399	29.25058058808351	30.10057382991534	24.07351658208717
17	15.674649496823662	29.288364891930748	28.090940368381602	26.946045242863985
18	20.130739835100698	28.241770394308396	25.831869063563644	25.79562070702727
19	21.891549832274553	32.97095216445695	25.755071698020473	19.382426305248025
20	23.206013540911492	30.688534460513868	24.024059078677364	22.081392919897276
21	19.453694260472087	32.78940319231289	24.753634051337503	23.003268495877517
22	18.71275327771156	30.316220832360568	25.108745069609135	25.862280820318738
23	21.34751729476672	30.738606342848012	21.46762837447624	26.44624798790902
24	17.644348328274948	30.84581546514628	26.392797021490978	25.117039185087798
25	18.273472346804613	29.316933511912808	27.109470036739857	25.30012410454272
26	21.436909428258975	32.64963198702432	23.373431797795607	22.540026786921104
27	17.660936559232272	29.988449676222306	26.655751201110796	25.694862563434622
28	21.632589115662977	27.69220844648145	29.814580440632565	20.86062199722301
29	21.565314623447158	29.839155597606382	27.381947089687035	21.21358268925943
30	20.05240652224666	29.04107737488173	28.91297936915572	21.99353673371589
31	28.938783283978225	28.870894412838062	22.325608542324566	19.86471376085915
32	26.25732646867282	27.12913016231891	25.44296720445302	21.170576164555253
33	24.46211125173562	28.89239767519015	25.80115011734637	20.844340955727855
34	23.41797426981065	26.221385301598616	27.71463327722006	22.64600715137068
35	24.88019610975265	25.975940921322632	29.237985820134426	19.90587714879029
36	28.278325940306942	25.48351621345981	28.748633006893332	17.48952483933991
37	24.48637921924726	29.46254131698266	26.091136969637397	19.959942494132683
38	25.822960569160635	28.37386186304265	27.143260877578857	18.65991669021786
39	25.546182863743045	25.218411707604783	28.00031947704066	21.235085951611516
40	26.650836169716037	26.751287123846502	23.662804271162283	22.93507243527518
41	24.11867343302655	22.874863300689334	29.03524077510045	23.971222491183664
42	28.178182175638643	23.58109187422435	25.524986790853127	22.715739159283878
43	24.13311133774867	24.38070604425986	27.37918238452748	24.10700023346399
44	23.006340390499243	23.30769325289065	26.658515906270353	27.027450450339753
45	25.6850325006451	25.764901760809998	27.06031972279223	21.489746015752676
46	21.792327635992777	24.071980634776306	32.78264502414509	21.35304670508583
47	22.841072459850338	24.890026172542175	26.220463733212096	26.048437634395388
48	24.39729427521718	21.563164297211948	33.056965213865304	20.982576213705563
49	23.155020090190824	21.91950407333227	30.38533846134942	24.540137375127482
50	20.887654669894204	22.560301291424498	27.571790177309758	28.98025386137154
51	20.723615497093988	23.032451494783924	32.217416413747344	24.026516594374748
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4064.0
1	3231.5
2	2399.0
3	1699.0
4	999.0
5	1144.0
6	1289.0
7	1235.0
8	1181.0
9	1184.5
10	1188.0
11	1201.0
12	1214.0
13	1262.5
14	1311.0
15	1281.0
16	1251.0
17	1295.0
18	1339.0
19	1346.5
20	1354.0
21	1259.0
22	1164.0
23	1465.5
24	1767.0
25	1726.0
26	2069.5
27	2454.0
28	2877.0
29	3300.0
30	3381.5
31	3463.0
32	4544.0
33	5625.0
34	5583.0
35	5541.0
36	6042.5
37	6544.0
38	7476.0
39	8408.0
40	10241.0
41	12074.0
42	14828.0
43	17582.0
44	23564.0
45	29546.0
46	31501.5
47	33457.0
48	35793.0
49	38129.0
50	34668.5
51	31208.0
52	26921.5
53	22635.0
54	20419.0
55	18203.0
56	15571.0
57	12939.0
58	12300.5
59	11662.0
60	10717.5
61	9773.0
62	9300.5
63	8828.0
64	7440.0
65	6052.0
66	5149.0
67	4246.0
68	3936.0
69	3626.0
70	3030.0
71	2434.0
72	2407.0
73	2380.0
74	1715.0
75	965.5
76	881.0
77	733.0
78	585.0
79	487.5
80	390.0
81	288.5
82	187.0
83	120.0
84	53.0
85	47.5
86	42.0
87	28.0
88	14.0
89	11.0
90	8.0
91	5.5
92	3.0
93	4.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	325532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.21248703492605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0901052074779	24.939291456545927
2	11.199227646640395	7.439084060304589
3	3.7603499458989638	3.746717214748626
4	1.773626412898208	2.3562617697273653
5	0.9865265985125219	1.6382500931353412
6	0.6928530554889619	1.3806823873521628
7	0.511886151071842	1.1900708509082223
8	0.4492573040098853	1.1936761907819315
9	0.34688061275940124	1.0368691068544937
>10	4.724129760220015	36.940350003974224
>50	0.40353390136515405	8.349078869594033
>100	0.05069021913700211	3.6978642059620572
>500	0.00496962932715707	1.2121547370373749
>1k	0.00496962932715707	3.215907257140007
>5k	9.939258654314141E-4	1.6637417959336518
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	5040	1.5482348893503557	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3501	1.0754703070665865	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC	1755	0.5391175061130703	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1645	0.5053266652740744	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGT	1611	0.49488222356020295	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1230	0.37784303847240824	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	828	0.25435287467898704	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	827	0.2540456852168143	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	801	0.24605875920032438	Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	647	0.19875158202573018	No Hit
AACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	569	0.1747908039762604	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	420	0.12901957411252965	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	382	0.11734637454996744	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	380	0.11673199562562207	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGTAGGTC	380	0.11673199562562207	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	365	0.1121241536930317	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	359	0.11028101691999558	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	356	0.1093594485334775	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	344	0.10567317498740524	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	344	0.10567317498740524	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	328	0.1007581435926422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.143789243453793E-4	0.0	0.0	1.7681825442660015	0.0
2	6.143789243453793E-4	0.0	0.0	2.371502647973164	0.0
3	6.143789243453793E-4	0.0	0.0	3.0414828649718	0.0
4	6.143789243453793E-4	0.0	0.0	5.774547509922219	0.0
5	6.143789243453793E-4	0.0	0.0	6.26113561800376	0.0
6	6.143789243453793E-4	0.0	0.0	8.30455992037649	0.0
7	6.143789243453793E-4	0.0	0.0	9.855866704348575	0.0
8	6.143789243453793E-4	0.0	0.0	10.792794563975278	0.0
9	6.143789243453793E-4	0.0	0.0	12.590160107147684	0.0
10	6.143789243453793E-4	0.0	0.0	14.586277232345822	0.0
11	6.143789243453793E-4	0.0	0.0	17.0588452133738	0.0
12	6.143789243453793E-4	0.0	0.0	18.242753400587347	0.0
13	6.143789243453793E-4	0.0	0.0	18.794158485187324	0.0
14	9.215683865180688E-4	0.0	0.0	19.385191010407578	0.0
15	9.215683865180688E-4	0.0	0.0	19.76794908027475	0.0
16	0.0012287578486907585	0.0	0.0	20.506432547337898	0.0
17	0.0012287578486907585	0.0	0.0	21.3702493149675	0.0
18	0.0012287578486907585	0.0	0.0	22.724647653686887	0.0
19	0.0012287578486907585	0.0	0.0	23.230588697885306	0.0
20	0.0012287578486907585	0.0	0.0	23.725163731983336	0.0
21	0.0012287578486907585	0.0	0.0	24.223732229089613	0.0
22	0.0012287578486907585	0.0	0.0	24.737967388766695	0.0
23	0.0012287578486907585	0.0	0.0	25.237150264797318	0.0
24	0.0012287578486907585	0.0	0.0	25.610692650799308	0.0
25	0.0012287578486907585	0.0	0.0	25.902522639863363	0.0
26	0.0012287578486907585	0.0	0.0	26.19896047086001	0.0
27	0.0012287578486907585	0.0	0.0	26.526731626998266	0.0
28	0.0012287578486907585	0.0	0.0	26.837914552179203	0.0
29	0.0012287578486907585	0.0	0.0	27.163228192620082	0.0
30	0.0012287578486907585	0.0	0.0	27.594522197510535	0.0
31	0.0012287578486907585	0.0	0.0	27.91829989064055	0.0
32	0.0012287578486907585	0.0	0.0	28.22057432141848	0.0
33	0.0012287578486907585	0.0	0.0	28.56094024550582	0.0
34	0.0012287578486907585	0.0	0.0	28.8457048769399	0.0
35	0.0012287578486907585	0.0	0.0	29.177162306624233	0.0
36	0.0012287578486907585	0.0	0.0	29.484658958259097	0.0
37	0.0012287578486907585	0.0	0.0	29.780175220869225	0.0
38	0.0012287578486907585	0.0	0.0	30.075998672941523	0.0
39	0.0012287578486907585	0.0	0.0	30.39639728198764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATTC	50	2.1827873E-11	45.0	42
CTTCGTC	20	7.0258294E-4	45.0	37
CGGGTTC	40	6.7884685E-9	45.0	6
ATCCTAA	20	7.0258294E-4	45.0	25
TCGTTGA	55	1.8189894E-12	45.0	24
ACACGTG	20	7.0258294E-4	45.0	42
TCACCGG	25	3.884625E-5	45.0	2
CTACTGA	20	7.0258294E-4	45.0	6
ATCTAGT	20	7.0258294E-4	45.0	10
GCGAATC	25	3.884625E-5	45.0	40
CTATCGC	20	7.0258294E-4	45.0	11
CATTGTC	20	7.0258294E-4	45.0	37
TTTCGAA	20	7.0258294E-4	45.0	31
GGCAATT	20	7.0258294E-4	45.0	8
TGGCGTG	20	7.0258294E-4	45.0	25
CGCATCG	35	1.2083365E-7	45.0	21
AATGGTC	85	0.0	45.0	9
TTACTAA	20	7.0258294E-4	45.0	17
GATCAAT	20	7.0258294E-4	45.0	9
ATAGACG	25	3.884625E-5	45.0	1
>>END_MODULE