SRR3552892_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333308
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	4912	1.4737120021121606	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3622	1.0866825878766786	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC	1813	0.5439413395418052	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1800	0.540041043119277	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGT	1745	0.5235397890239658	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1268	0.3804289125973574	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	881	0.26432008832671283	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	830	0.24901892543833332	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	810	0.24301846940367466	Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	616	0.18481404586748593	No Hit
AACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	589	0.17671343022069677	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	452	0.13561030638328514	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	386	0.11580880146891163	Illumina Single End Adapter 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	378	0.11340861905504819	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	376	0.11280857345158232	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGTAGGTC	367	0.11010836823598592	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	366	0.10980834543425301	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	365	0.10950832263252007	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	362	0.10860825422732129	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	349	0.10470795780479315	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	345	0.10350786659786143	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	340	0.10200775258919678	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCATT	25	3.884826E-5	45.000004	9
CTACGAA	25	3.884826E-5	45.000004	10
CGAATAT	25	3.884826E-5	45.000004	13
CTATTAC	25	3.884826E-5	45.000004	32
TAAGGTT	25	3.884826E-5	45.000004	16
GCTACGA	25	3.884826E-5	45.000004	9
TCGACGT	25	3.884826E-5	45.000004	26
GTAGTGC	25	3.884826E-5	45.000004	29
CTTACAG	25	3.884826E-5	45.000004	1
CATATAG	20	7.026071E-4	45.0	1
AACCACG	40	6.7902874E-9	45.0	1
CGCGCCA	20	7.026071E-4	45.0	32
CGACAAT	35	1.2084638E-7	45.0	20
ATCACGC	20	7.026071E-4	45.0	42
ATCGTTG	70	0.0	45.0	23
ATCCGTT	20	7.026071E-4	45.0	10
CGCCTGC	40	6.7902874E-9	45.0	19
GCCGATG	20	7.026071E-4	45.0	9
ATATCGC	20	7.026071E-4	45.0	33
CCCGTAG	20	7.026071E-4	45.0	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content