##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333308
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217867557934404	31.0	31.0	33.0	30.0	34.0
2	31.488299110732417	31.0	31.0	34.0	30.0	34.0
3	31.669848908517046	31.0	31.0	34.0	30.0	34.0
4	35.57940103447862	37.0	35.0	37.0	35.0	37.0
5	34.90529180217697	35.0	35.0	37.0	32.0	37.0
6	34.987975086106545	35.0	35.0	37.0	32.0	37.0
7	34.9015235157872	36.0	35.0	37.0	32.0	37.0
8	35.27116360843424	37.0	35.0	37.0	33.0	37.0
9	36.81895124029426	39.0	37.0	39.0	33.0	39.0
10	36.33311831699209	38.0	35.0	39.0	32.0	39.0
11	36.49525063904857	38.0	35.0	39.0	32.0	39.0
12	36.126657625979576	37.0	35.0	39.0	32.0	39.0
13	35.804997179785666	37.0	35.0	39.0	30.0	39.0
14	36.85136570379349	39.0	35.0	40.0	31.0	41.0
15	36.841563958860874	38.0	35.0	40.0	32.0	41.0
16	36.735814921934065	38.0	35.0	40.0	31.0	41.0
17	36.63258607654182	38.0	35.0	40.0	31.0	41.0
18	36.49624071429429	38.0	35.0	40.0	31.0	41.0
19	36.570157331957226	38.0	35.0	40.0	31.0	41.0
20	36.39212680163693	38.0	35.0	40.0	30.0	41.0
21	36.50298522687724	38.0	35.0	40.0	31.0	41.0
22	36.49173737204028	38.0	35.0	40.0	31.0	41.0
23	36.39309887551454	38.0	35.0	40.0	31.0	41.0
24	35.97012372940343	38.0	34.0	40.0	30.0	41.0
25	36.1439929434637	38.0	35.0	40.0	30.0	41.0
26	36.00804061108644	38.0	34.0	40.0	30.0	41.0
27	35.86285657709986	38.0	34.0	40.0	29.0	41.0
28	35.84649933394938	38.0	34.0	40.0	29.0	41.0
29	35.46740252259172	38.0	34.0	40.0	27.0	41.0
30	35.509687736267956	38.0	34.0	40.0	28.0	41.0
31	35.47228089334789	38.0	34.0	40.0	28.0	41.0
32	35.35344186158148	38.0	34.0	40.0	27.0	41.0
33	35.19274964897332	38.0	34.0	40.0	27.0	41.0
34	35.20390749696977	38.0	34.0	40.0	27.0	41.0
35	35.07727387281433	38.0	34.0	40.0	26.0	41.0
36	34.90081846220313	37.0	34.0	40.0	25.0	41.0
37	34.77158364035667	37.0	34.0	40.0	25.0	41.0
38	34.704450538240906	37.0	33.0	40.0	25.0	41.0
39	34.663527428084535	37.0	33.0	40.0	25.0	41.0
40	34.43521307619379	37.0	33.0	40.0	24.0	41.0
41	34.52467987567055	37.0	33.0	40.0	25.0	41.0
42	34.499645973093955	37.0	33.0	40.0	24.0	41.0
43	34.408103015829205	37.0	33.0	40.0	24.0	41.0
44	34.26966049419756	37.0	33.0	40.0	23.0	41.0
45	34.14562206727711	37.0	33.0	40.0	23.0	41.0
46	34.106322080478115	36.0	33.0	40.0	23.0	41.0
47	33.99575167712746	36.0	33.0	40.0	23.0	41.0
48	33.994398574291644	36.0	33.0	40.0	23.0	41.0
49	33.81691408547049	36.0	33.0	39.0	23.0	41.0
50	33.714033266528254	36.0	33.0	39.0	23.0	40.0
51	32.391967789552005	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	9.0
11	6.0
12	7.0
13	15.0
14	24.0
15	38.0
16	79.0
17	193.0
18	365.0
19	716.0
20	1201.0
21	1839.0
22	2485.0
23	2948.0
24	3343.0
25	3464.0
26	3829.0
27	4351.0
28	5047.0
29	6327.0
30	8090.0
31	10825.0
32	14481.0
33	20595.0
34	32310.0
35	34948.0
36	37021.0
37	50158.0
38	57463.0
39	31128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.29656653905697	34.640632688084295	19.529684256003456	26.53311651685528
2	22.108680259699735	35.821822458506844	20.07152543593313	21.997971845860285
3	20.25363927658502	34.210700013201	20.93829131013957	24.597369400074406
4	20.852484788843952	34.20889987639061	20.088926758433644	24.8496885763318
5	16.640464675315325	38.232805693232685	18.277089058768468	26.849640572683526
6	19.419275864965737	37.091818978242344	22.083178321552438	21.405726835239477
7	71.73425180313704	12.41974390053644	8.950880266900283	6.895124029426237
8	74.12213328212944	9.461819098251468	7.548873714402295	8.867173905216797
9	68.6428168540809	12.613858653257648	12.501950148211263	6.241374344450178
10	36.804397134182196	31.743912537352838	13.996963769246463	17.4547265592185
11	29.801864941735573	27.63600033602554	23.988623135358285	18.573511586880603
12	29.82916701669327	24.075329725059106	28.62667562734768	17.46882763089995
13	23.535888727543295	32.20774778883195	23.332473267968364	20.92389021565639
14	16.466151427508493	33.79126813637836	30.354806965329367	19.387773470783777
15	15.477876318600215	30.93235085866526	31.470891787775873	22.118881034958658
16	16.534256603501866	29.127713706241675	30.179893671919068	24.158136018337395
17	15.71699449158136	29.47003972301895	28.155339805825243	26.657625979574444
18	19.94881611002436	28.650077405882847	25.652249570967395	25.748856913125394
19	21.65774598869514	33.44024145835083	25.605446013897055	19.29656653905697
20	23.06275276921046	31.057760389789625	23.881214972337897	21.998271868662016
21	19.448978122337298	32.894800004800366	24.55986654985779	23.09635532300455
22	18.636816398046253	30.54052107960205	24.995499657974005	25.827162864377694
23	21.331921226013176	30.929050607846197	21.40062644761002	26.33840171853061
24	17.70734575827763	31.159168096775353	26.1487873078354	24.984698837111623
25	18.370696172909142	29.486540977114263	26.83493945539861	25.307823394577987
26	21.527836115544783	32.597777431084765	23.303971101803736	22.57041535156672
27	17.44032546473532	30.200895268040373	26.609622331297178	25.749156935927132
28	21.656245874686476	27.67500330025082	29.768862433544953	20.899888391517756
29	21.584840447874036	29.84986859001284	27.286473772006676	21.278817190106448
30	20.085326484812846	29.05750837063617	28.85709313907857	22.000072005472415
31	28.934499021925664	29.02390581684208	22.122481308579452	19.9191138526528
32	26.22709325908769	27.19586688588333	25.467735547901643	21.109304307127342
33	24.41165528580172	29.01160488197103	25.647749228941404	20.92899060328585
34	23.562290734095793	26.27809713538229	27.59399714378293	22.565614986738993
35	24.99189938435321	25.951972349898593	28.99780383309131	20.05832443265688
36	28.30415111548478	25.477336277557093	28.75148511286858	17.467027494089553
37	24.61297058576452	29.435837123621393	25.909969157655983	20.041223132958105
38	25.747356799116734	28.373756405486816	27.081858221224813	18.797028574171637
39	25.54724159036087	25.38012888979562	27.935423092155006	21.137206427688504
40	26.65642588836752	26.962149123333372	23.51938747344798	22.862037514851128
41	24.136234353810888	22.960444993819532	28.938399318348196	23.964921334021387
42	28.25314723919018	23.701501314099872	25.261619883111113	22.783731563598835
43	24.177937523251767	24.468059572527512	27.082158244026544	24.271844660194176
44	22.978446361923506	23.462383141118725	26.65672591116925	26.902444585788523
45	25.70805381208972	25.890767698345073	26.72033074513663	21.680847744428576
46	21.781955428612573	24.190838503726283	32.652081558198425	21.37512450946272
47	22.949644172957147	24.888091494953617	26.17998967921562	25.982274652873617
48	24.305447213988263	21.522435705113587	32.95150431432789	21.22061276657026
49	23.168960841023917	21.72945143830931	30.505418411799294	24.596169308867474
50	21.007596577339875	22.548513687040213	27.458686860201375	28.98520287541853
51	20.615166752673204	23.09665534580628	32.31125565542981	23.976922246090705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4191.0
1	3315.0
2	2439.0
3	1715.5
4	992.0
5	1112.0
6	1232.0
7	1221.0
8	1210.0
9	1226.0
10	1242.0
11	1286.0
12	1330.0
13	1330.5
14	1331.0
15	1334.0
16	1337.0
17	1344.0
18	1351.0
19	1338.5
20	1326.0
21	1289.5
22	1253.0
23	1586.5
24	1920.0
25	1850.5
26	2181.5
27	2582.0
28	3059.0
29	3536.0
30	3485.0
31	3434.0
32	4406.0
33	5378.0
34	5560.5
35	5743.0
36	6264.5
37	6786.0
38	7624.0
39	8462.0
40	10399.5
41	12337.0
42	15249.0
43	18161.0
44	24404.0
45	30647.0
46	32739.5
47	34832.0
48	36859.5
49	38887.0
50	35469.5
51	32052.0
52	27543.5
53	23035.0
54	20711.5
55	18388.0
56	15710.5
57	13033.0
58	12341.5
59	11650.0
60	10837.0
61	10024.0
62	9499.5
63	8975.0
64	7543.0
65	6111.0
66	5189.5
67	4268.0
68	4029.5
69	3791.0
70	3136.0
71	2481.0
72	2459.5
73	2438.0
74	1754.0
75	1013.0
76	956.0
77	793.0
78	630.0
79	515.0
80	400.0
81	280.0
82	160.0
83	108.5
84	57.0
85	52.0
86	47.0
87	28.5
88	10.0
89	6.5
90	3.0
91	5.0
92	7.0
93	3.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	333308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.57316567206625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43161397600994	29.844971915202343
2	9.286852064990859	6.9787286241974416
3	3.1683622909884046	3.571362038053041
4	1.419724511405795	2.13374177102973
5	0.7991173534096894	1.5012684355542687
6	0.5923428662702025	1.3353717989422142
7	0.44358501114469645	1.1666825179369524
8	0.33087717049110993	0.9945682195173589
9	0.28451960128321424	0.9621271904367998
>10	3.8322113101334216	33.85091480300474
>50	0.354849156963962	8.285257992091235
>100	0.047153378965931886	3.7037574598402845
>500	0.003996049064909482	1.118874679371242
>1k	0.004795258877891379	4.5523725548223375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	4912	1.4737120021121606	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3622	1.0866825878766786	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC	1813	0.5439413395418052	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1800	0.540041043119277	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGT	1745	0.5235397890239658	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1268	0.3804289125973574	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	881	0.26432008832671283	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	830	0.24901892543833332	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	810	0.24301846940367466	Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	616	0.18481404586748593	No Hit
AACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	589	0.17671343022069677	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	452	0.13561030638328514	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	386	0.11580880146891163	Illumina Single End Adapter 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	378	0.11340861905504819	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	376	0.11280857345158232	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGTAGGTC	367	0.11010836823598592	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	366	0.10980834543425301	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	365	0.10950832263252007	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	362	0.10860825422732129	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	349	0.10470795780479315	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	345	0.10350786659786143	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	340	0.10200775258919678	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.000456034658634E-4	0.0	0.0	1.7158304031106364	0.0
2	6.000456034658634E-4	0.0	0.0	2.327276873042351	0.0
3	6.000456034658634E-4	0.0	0.0	3.0053284049587767	0.0
4	6.000456034658634E-4	0.0	0.0	5.842644040947112	0.0
5	6.000456034658634E-4	0.0	0.0	6.32958104815966	0.0
6	6.000456034658634E-4	0.0	0.0	8.414439497401803	0.0
7	6.000456034658634E-4	0.0	0.0	9.97785831723211	0.0
8	6.000456034658634E-4	0.0	0.0	10.928030530320305	0.0
9	6.000456034658634E-4	0.0	0.0	12.790572083478345	0.0
10	6.000456034658634E-4	0.0	0.0	14.81032558474444	0.0
11	6.000456034658634E-4	0.0	0.0	17.263011988911156	0.0
12	6.000456034658634E-4	0.0	0.0	18.46340321864462	0.0
13	6.000456034658634E-4	0.0	0.0	18.998043851332703	0.0
14	6.000456034658634E-4	0.0	0.0	19.561486672987147	0.0
15	6.000456034658634E-4	0.0	0.0	19.94041547157584	0.0
16	6.000456034658634E-4	0.0	0.0	20.677871518235385	0.0
17	6.000456034658634E-4	0.0	0.0	21.50113408619055	0.0
18	6.000456034658634E-4	0.0	0.0	22.83143518907437	0.0
19	6.000456034658634E-4	0.0	0.0	23.32077237870078	0.0
20	6.000456034658634E-4	0.0	0.0	23.801408907076937	0.0
21	6.000456034658634E-4	0.0	0.0	24.313847852436787	0.0
22	6.000456034658634E-4	0.0	0.0	24.815185954132513	0.0
23	6.000456034658634E-4	0.0	0.0	25.32732487669063	0.0
24	6.000456034658634E-4	0.0	0.0	25.70715374368452	0.0
25	6.000456034658634E-4	0.0	0.0	26.016477252271173	0.0
26	6.000456034658634E-4	0.0	0.0	26.312599757581577	0.0
27	6.000456034658634E-4	0.0	0.0	26.648925318324192	0.0
28	6.000456034658634E-4	0.0	0.0	26.958248826910847	0.0
29	6.000456034658634E-4	0.0	0.0	27.26367203907497	0.0
30	6.000456034658634E-4	0.0	0.0	27.693004668354796	0.0
31	6.000456034658634E-4	0.0	0.0	28.017329317028093	0.0
32	6.000456034658634E-4	0.0	0.0	28.329353030830344	0.0
33	6.000456034658634E-4	0.0	0.0	28.664478500366027	0.0
34	6.000456034658634E-4	0.0	0.0	28.934198999123932	0.0
35	6.000456034658634E-4	0.0	0.0	29.266324240642287	0.0
36	6.000456034658634E-4	0.0	0.0	29.563646837159624	0.0
37	6.000456034658634E-4	0.0	0.0	29.855569023245767	0.0
38	6.000456034658634E-4	0.0	0.0	30.148991323340574	0.0
39	6.000456034658634E-4	0.0	0.0	30.440013441021517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCATT	25	3.884826E-5	45.000004	9
CTACGAA	25	3.884826E-5	45.000004	10
CGAATAT	25	3.884826E-5	45.000004	13
CTATTAC	25	3.884826E-5	45.000004	32
TAAGGTT	25	3.884826E-5	45.000004	16
GCTACGA	25	3.884826E-5	45.000004	9
TCGACGT	25	3.884826E-5	45.000004	26
GTAGTGC	25	3.884826E-5	45.000004	29
CTTACAG	25	3.884826E-5	45.000004	1
CATATAG	20	7.026071E-4	45.0	1
AACCACG	40	6.7902874E-9	45.0	1
CGCGCCA	20	7.026071E-4	45.0	32
CGACAAT	35	1.2084638E-7	45.0	20
ATCACGC	20	7.026071E-4	45.0	42
ATCGTTG	70	0.0	45.0	23
ATCCGTT	20	7.026071E-4	45.0	10
CGCCTGC	40	6.7902874E-9	45.0	19
GCCGATG	20	7.026071E-4	45.0	9
ATATCGC	20	7.026071E-4	45.0	33
CCCGTAG	20	7.026071E-4	45.0	23
>>END_MODULE