FastQCFastQC Report
Sat 18 Jun 2016
SRR3552889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444014
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC71531.6109852392041693No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT60061.352660051259645No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGT59191.3330660744931466No Hit
AATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT55051.2398257712594647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40030.9015481493826771No Hit
AATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT13110.29526096023999243No Hit
AAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT12950.29165747025994676No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT11940.26891043976090845No Hit
AACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG10570.23805555680676735No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTAT10270.23129901309418172No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9520.2144076538127176No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT8750.1970658582837478No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGTC7980.179724062754778No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7080.15945443161702108No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC6970.1569770322557397No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.11846473309400155No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT4800.10810469940137023No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4450.10022206507002032No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTT207.0286036E-445.00000419
CTCGTCA207.0286036E-445.00000436
ACGTTTG207.0286036E-445.0000041
GAGTCTA207.0286036E-445.0000049
ATTACCG207.0286036E-445.00000425
TGCGGTA207.0286036E-445.00000412
CATTCGG207.0286036E-445.0000042
ATCACGC207.0286036E-445.00000442
ACGGGCG650.045.0000045
TGCATCA207.0286036E-445.00000413
GCATTAC207.0286036E-445.00000423
CCACTAG207.0286036E-445.0000041
TCTACCG207.0286036E-445.00000416
CTAAACA207.0286036E-445.00000426
TGCGAAC207.0286036E-445.00000414
TCTAACG207.0286036E-445.0000041
GGTATGC800.045.0000048
GTACTAG207.0286036E-445.0000041
ACAGACG207.0286036E-445.0000041
TTACGCG207.0286036E-445.0000041