##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444014
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.980077204772822	31.0	31.0	33.0	28.0	34.0
2	31.274851693865507	31.0	31.0	34.0	28.0	34.0
3	31.46677582238398	31.0	31.0	34.0	30.0	34.0
4	35.424605980892494	37.0	35.0	37.0	33.0	37.0
5	34.72172949501592	35.0	35.0	37.0	32.0	37.0
6	34.85287400847721	35.0	35.0	37.0	32.0	37.0
7	34.73484619854329	35.0	35.0	37.0	32.0	37.0
8	35.20217380533046	37.0	35.0	37.0	32.0	37.0
9	36.69988558919314	39.0	35.0	39.0	32.0	39.0
10	36.12760183237466	37.0	35.0	39.0	32.0	39.0
11	36.347358416626506	38.0	35.0	39.0	32.0	39.0
12	35.854718544910746	37.0	35.0	39.0	31.0	39.0
13	35.44801290049413	37.0	35.0	39.0	30.0	39.0
14	36.44969527987856	38.0	35.0	40.0	31.0	41.0
15	36.542780182606855	38.0	35.0	40.0	31.0	41.0
16	36.413831545852155	38.0	35.0	40.0	31.0	41.0
17	36.353955055471225	38.0	35.0	40.0	31.0	41.0
18	36.24795614552694	38.0	35.0	40.0	30.0	41.0
19	36.25578247532736	38.0	34.0	40.0	30.0	41.0
20	36.19110433454801	38.0	34.0	40.0	30.0	41.0
21	36.310433905237225	38.0	34.0	40.0	30.0	41.0
22	36.27188331899445	38.0	34.0	40.0	30.0	41.0
23	36.189203493583534	38.0	34.0	40.0	30.0	41.0
24	35.68104384095997	38.0	34.0	40.0	29.0	41.0
25	35.98465814140996	38.0	34.0	40.0	30.0	41.0
26	35.77270086078367	38.0	34.0	40.0	30.0	41.0
27	35.713009049264215	38.0	34.0	40.0	29.0	41.0
28	35.79141198250505	38.0	34.0	40.0	29.0	41.0
29	35.23982351907823	37.0	34.0	40.0	27.0	41.0
30	35.486378807875425	38.0	34.0	40.0	29.0	41.0
31	35.28055646894017	37.0	34.0	40.0	27.0	41.0
32	35.264358781479864	37.0	34.0	40.0	27.0	41.0
33	35.31660713400929	38.0	34.0	40.0	28.0	41.0
34	35.15663920507011	38.0	34.0	40.0	27.0	41.0
35	35.130588224695614	38.0	34.0	40.0	27.0	41.0
36	35.01475403928705	37.0	34.0	40.0	26.0	41.0
37	35.01565491178206	37.0	34.0	40.0	26.0	41.0
38	34.966534838991564	37.0	34.0	40.0	26.0	41.0
39	34.90087699937389	37.0	34.0	40.0	26.0	41.0
40	34.63855869409523	37.0	33.0	40.0	25.0	41.0
41	34.799145972874726	37.0	34.0	40.0	26.0	41.0
42	34.72134887638678	37.0	33.0	40.0	26.0	41.0
43	34.692586269802305	37.0	33.0	40.0	26.0	41.0
44	34.65155152765453	37.0	33.0	40.0	25.0	41.0
45	34.439736134446214	37.0	33.0	40.0	24.0	41.0
46	34.37750161030959	37.0	33.0	40.0	24.0	41.0
47	34.27633588130104	36.0	33.0	40.0	24.0	41.0
48	34.34356349124127	36.0	33.0	40.0	24.0	41.0
49	34.26285657659443	36.0	33.0	40.0	24.0	41.0
50	34.15650857855833	36.0	33.0	39.0	24.0	41.0
51	32.88049250699302	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	12.0
11	12.0
12	10.0
13	17.0
14	41.0
15	71.0
16	101.0
17	187.0
18	482.0
19	839.0
20	1410.0
21	2132.0
22	2692.0
23	3400.0
24	3775.0
25	4163.0
26	4711.0
27	5683.0
28	7156.0
29	9070.0
30	11926.0
31	15570.0
32	21144.0
33	29793.0
34	46396.0
35	47905.0
36	49009.0
37	63959.0
38	72304.0
39	40037.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.048304783182513	38.08798821658777	19.327318507974976	24.536388492254748
2	21.48648466039359	37.20152968149653	19.69780232154842	21.61418333656146
3	19.619426414482426	30.701734629989147	24.93817762502984	24.740661330498586
4	23.33192196642448	32.27105451629904	19.345561175998956	25.051462341277524
5	16.89856626142419	38.75440864477247	18.285009031246762	26.062016062556587
6	19.943290076439034	35.45586400428815	23.619975946704383	20.980869972568435
7	70.2806668258208	13.403180980779887	9.387992270513992	6.928159922885315
8	72.89544924259145	7.613048237217745	7.2844549946623305	12.207047525528475
9	69.95590229136918	10.45057137837996	12.576405248483155	7.017121081767693
10	39.326462679104715	29.90378681753278	14.031989982297855	16.73776052106465
11	31.107352470868037	24.192930853531646	24.81092938510948	19.88878729049084
12	30.46075123757359	23.54475309337093	29.558527433819652	16.435968235235826
13	24.31342254973942	33.18949402496318	22.02317944929664	20.473903976000756
14	15.66797443323859	31.58819316508038	32.48658826073052	20.25724414095051
15	13.921182665411452	29.667983441963543	31.374235947515167	25.03659794510984
16	16.382141103658892	27.68696482543343	33.25570815334651	22.67518591756116
17	15.804681834356574	27.2977879075885	29.48037674487741	27.41715351317751
18	19.287229681946965	26.29331507565077	28.178615989585914	26.24083925281635
19	20.201390046259803	32.50888485498205	28.88039566319981	18.409329435558337
20	23.516826046025578	31.75845806663754	24.445175152134844	20.279540735202044
21	18.766525379830366	31.269734738093845	26.557495934812863	23.406243947262926
22	18.788146319710638	30.248370546874646	26.509749692577262	24.453733440837453
23	21.73242285153171	29.96279396595603	21.532654375762927	26.772128806749336
24	16.942934231803502	33.12485642344611	27.041489682757753	22.89071966199264
25	17.710928033800734	26.969194665033086	26.829784646430067	28.49009265473611
26	20.20589440873486	34.24171309913651	22.59095433927759	22.961438152851034
27	14.413959920182698	30.40332061601661	29.23331246312053	25.949407000680157
28	18.057989162503883	28.141454999166694	29.094352880765022	24.7062029575644
29	17.2267090677321	32.63478178615989	27.631786385113983	22.506722760994023
30	19.923921317796285	27.233825960442687	28.777921416892262	24.064331304868766
31	23.877625480277647	29.556050034458377	23.464800659438666	23.101523825825314
32	23.131928272531947	26.49646182327584	23.108730805785402	27.262879098406806
33	21.979261915164837	25.36744336890278	28.548874585035605	24.104420130896774
34	17.40936096609566	26.955906795731664	24.355087902633702	31.27964433553897
35	20.44732823739792	25.650092114212615	28.893233096253724	25.009346552135742
36	19.960181435720497	27.666920412419426	30.887990018332758	21.484908133527323
37	19.78788957104956	27.67795610048332	26.82235244834622	25.7118018801209
38	20.9624020864207	26.052556901358965	31.033255708153344	21.95178530406699
39	19.417630975599867	24.33053912714464	27.965559644515714	28.286270252739776
40	23.81771745935939	24.95777160179634	25.51924038431221	25.705270554532067
41	19.196466778074566	21.398199155882473	30.726959059849463	28.678375006193495
42	25.598066727625707	23.63799339660461	23.57087839572626	27.193061480043422
43	20.494398825262266	23.54385222087592	28.909223583040173	27.05252537082164
44	19.852752390690384	22.916394528100465	27.780880783038377	29.449972298170778
45	24.39923065488926	26.675960667906867	26.32056646862486	22.60424220857901
46	18.842649105658833	24.234596206425923	33.028237848356135	23.894516839559113
47	20.547325084344187	26.02080114590981	25.928912151418647	27.502961618327348
48	20.740562234524138	21.04145364785795	34.76399392811938	23.45399018949853
49	23.69182052818154	20.34237659172909	30.453319039489745	25.51248384059962
50	19.568076682266774	21.98331584139239	27.396658663916	31.051948812424833
51	17.90371474773318	22.564378600674754	33.284310855063126	26.247595796528937
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4572.0
1	3609.5
2	2647.0
3	1800.5
4	954.0
5	1148.0
6	1342.0
7	1227.5
8	1113.0
9	1105.5
10	1098.0
11	1075.5
12	1053.0
13	1132.5
14	1212.0
15	1250.5
16	1289.0
17	1307.0
18	1325.0
19	1380.0
20	1435.0
21	1454.0
22	1473.0
23	1791.5
24	2110.0
25	2517.0
26	2971.5
27	3019.0
28	3774.0
29	4529.0
30	5188.0
31	5847.0
32	6653.0
33	7459.0
34	8261.0
35	9063.0
36	9360.5
37	9658.0
38	10553.5
39	11449.0
40	14413.5
41	17378.0
42	22138.0
43	26898.0
44	37575.5
45	48253.0
46	50386.5
47	52520.0
48	51302.0
49	50084.0
50	44695.0
51	39306.0
52	34848.5
53	30391.0
54	26826.0
55	23261.0
56	20736.5
57	18212.0
58	16560.0
59	14908.0
60	13716.5
61	12525.0
62	11193.5
63	9862.0
64	9259.0
65	8656.0
66	6939.0
67	5222.0
68	4442.0
69	3662.0
70	3275.5
71	2889.0
72	2258.0
73	1627.0
74	1475.5
75	1012.5
76	701.0
77	525.5
78	350.0
79	307.5
80	265.0
81	183.0
82	101.0
83	65.5
84	30.0
85	18.5
86	7.0
87	5.0
88	3.0
89	3.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.254936570504746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.21700176952497	25.5513936738159
2	9.228021157218654	5.95298474194727
3	3.203524126058442	3.0998840246429014
4	1.4463095215459305	1.8660248751512425
5	0.8449073452473419	1.3626216414453285
6	0.5885271018655545	1.1389742604417867
7	0.4009379636843868	0.9052560011143062
8	0.32073170552343516	0.8276144654246568
9	0.2701525402905587	0.7842377746289428
>10	3.5656696399863144	27.746612314703285
>50	0.8251107780261144	17.43866470530834
>100	0.07788035404838325	4.493810621401141
>500	0.004209748867480176	1.031062153955965
>1k	0.004209748867480176	2.2375135022306027
>5k	0.002806499244986784	5.5633452437883
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	7153	1.6109852392041693	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	6006	1.352660051259645	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGT	5919	1.3330660744931466	No Hit
AATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	5505	1.2398257712594647	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4003	0.9015481493826771	No Hit
AATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT	1311	0.29526096023999243	No Hit
AAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	1295	0.29165747025994676	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	1194	0.26891043976090845	No Hit
AACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1057	0.23805555680676735	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTAT	1027	0.23129901309418172	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	952	0.2144076538127176	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT	875	0.1970658582837478	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGTC	798	0.179724062754778	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	708	0.15945443161702108	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	697	0.1569770322557397	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	526	0.11846473309400155	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	480	0.10810469940137023	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	445	0.10022206507002032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2521812375285463E-4	0.0	0.0	1.6186426554117663	0.0
2	2.2521812375285463E-4	0.0	0.0	2.0530884161310228	0.0
3	2.2521812375285463E-4	0.0	0.0	2.752841126631142	0.0
4	2.2521812375285463E-4	0.0	0.0	6.230434175498971	0.0
5	2.2521812375285463E-4	0.0	0.0	6.630196345160288	0.0
6	2.2521812375285463E-4	0.0	0.0	8.625854139734333	0.0
7	2.2521812375285463E-4	0.0	0.0	10.203957532870586	0.0
8	2.2521812375285463E-4	0.0	0.0	11.074425581175369	0.0
9	4.5043624750570925E-4	0.0	0.0	13.782673519303446	0.0
10	4.5043624750570925E-4	0.0	0.0	15.547257518907061	0.0
11	4.5043624750570925E-4	0.0	0.0	19.230024278513742	0.0
12	4.5043624750570925E-4	0.0	0.0	20.27165810087069	0.0
13	4.5043624750570925E-4	0.0	0.0	20.7416883251429	0.0
14	4.5043624750570925E-4	0.0	0.0	21.44954888809812	0.0
15	4.5043624750570925E-4	0.0	0.0	21.827014463507908	0.0
16	4.5043624750570925E-4	0.0	0.0	22.51910975780043	0.0
17	4.5043624750570925E-4	0.0	0.0	23.31052624466796	0.0
18	4.5043624750570925E-4	0.0	0.0	24.615890489939506	0.0
19	4.5043624750570925E-4	0.0	0.0	25.117000815289607	0.0
20	4.5043624750570925E-4	0.0	0.0	25.533429126108636	0.0
21	4.5043624750570925E-4	0.0	0.0	26.03634119644876	0.0
22	4.5043624750570925E-4	0.0	0.0	26.52551496123996	0.0
23	4.5043624750570925E-4	0.0	0.0	27.003202601719767	0.0
24	4.5043624750570925E-4	0.0	0.0	27.34823676730914	0.0
25	4.5043624750570925E-4	0.0	0.0	27.66782128491444	0.0
26	4.5043624750570925E-4	0.0	0.0	27.950244812100518	0.0
27	4.5043624750570925E-4	0.0	0.0	28.25068578918683	0.0
28	6.75654371258564E-4	0.0	0.0	28.52387537329904	0.0
29	6.75654371258564E-4	0.0	0.0	28.79706495741125	0.0
30	6.75654371258564E-4	0.0	0.0	29.21574544946781	0.0
31	6.75654371258564E-4	0.0	0.0	29.501997684757686	0.0
32	6.75654371258564E-4	0.0	0.0	29.792979500646375	0.0
33	6.75654371258564E-4	0.0	0.0	30.1024292026828	0.0
34	6.75654371258564E-4	0.0	0.0	30.36931267932993	0.0
35	6.75654371258564E-4	0.0	0.0	30.71975207988937	0.0
36	6.75654371258564E-4	0.0	0.0	30.989563392145293	0.0
37	9.008724950114185E-4	0.0	0.0	31.252392942564875	0.0
38	9.008724950114185E-4	0.0	0.0	31.572878332665187	0.0
39	9.008724950114185E-4	0.0	0.0	31.874895836617764	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTT	20	7.0286036E-4	45.000004	19
CTCGTCA	20	7.0286036E-4	45.000004	36
ACGTTTG	20	7.0286036E-4	45.000004	1
GAGTCTA	20	7.0286036E-4	45.000004	9
ATTACCG	20	7.0286036E-4	45.000004	25
TGCGGTA	20	7.0286036E-4	45.000004	12
CATTCGG	20	7.0286036E-4	45.000004	2
ATCACGC	20	7.0286036E-4	45.000004	42
ACGGGCG	65	0.0	45.000004	5
TGCATCA	20	7.0286036E-4	45.000004	13
GCATTAC	20	7.0286036E-4	45.000004	23
CCACTAG	20	7.0286036E-4	45.000004	1
TCTACCG	20	7.0286036E-4	45.000004	16
CTAAACA	20	7.0286036E-4	45.000004	26
TGCGAAC	20	7.0286036E-4	45.000004	14
TCTAACG	20	7.0286036E-4	45.000004	1
GGTATGC	80	0.0	45.000004	8
GTACTAG	20	7.0286036E-4	45.000004	1
ACAGACG	20	7.0286036E-4	45.000004	1
TTACGCG	20	7.0286036E-4	45.000004	1
>>END_MODULE