SRR3552887_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290242
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	3019	1.040166481763494	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2659	0.9161320553193542	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC	2392	0.8241398557066173	No Hit
AATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	2060	0.709752551319244	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGT	2041	0.703206289923581	No Hit
AAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	822	0.28321194038078573	TruSeq Adapter, Index 15 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	693	0.23876627090496896	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	570	0.1963878418698879	No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	511	0.1760599775359872	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	478	0.16469015511194107	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	455	0.15676573342245437	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	441	0.15194217239407115	No Hit
AACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTG	425	0.14642953121877605	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	417	0.1436732106311285	TruSeq Adapter, Index 15 (95% over 23bp)
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	367	0.12644620695833134	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT	365	0.12575712681141943	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	321	0.11059736357935791	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	309	0.1064628826978866	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTATC	298	0.10267294188987121	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	75	0.0	45.000004	5
TACCAGA	25	3.883576E-5	45.000004	19
TAGACTG	25	3.883576E-5	45.000004	1
TTAGCCG	25	3.883576E-5	45.000004	23
GCGCGCC	35	1.2077362E-7	45.000004	16
CGCGCCT	35	1.2077362E-7	45.000004	17
ACCCTTG	25	3.883576E-5	45.000004	28
TACTGCG	25	3.883576E-5	45.000004	1
ACGTGGT	35	1.2077362E-7	45.000004	14
ATTGGGT	25	3.883576E-5	45.000004	4
GTTGGGA	35	1.2077362E-7	45.000004	4
AATACGG	35	1.2077362E-7	45.000004	2
CCCTGCG	20	7.0245634E-4	45.0	1
GTCATAC	45	3.8198777E-10	45.0	39
CGGGTAC	45	3.8198777E-10	45.0	6
CGGCGCG	20	7.0245634E-4	45.0	14
GATACGA	20	7.0245634E-4	45.0	9
GTCGCAA	20	7.0245634E-4	45.0	15
TACCCGA	20	7.0245634E-4	45.0	28
TCTCTAG	20	7.0245634E-4	45.0	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content