##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290242
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09662626360072	31.0	31.0	33.0	28.0	34.0
2	31.371365963575222	31.0	31.0	34.0	30.0	34.0
3	31.55477842627876	31.0	31.0	34.0	30.0	34.0
4	35.50337304731914	37.0	35.0	37.0	33.0	37.0
5	34.86978107923733	35.0	35.0	37.0	32.0	37.0
6	34.98683167839251	35.0	35.0	37.0	32.0	37.0
7	34.932446027797496	36.0	35.0	37.0	32.0	37.0
8	35.37042536917469	37.0	35.0	37.0	33.0	37.0
9	36.83057241887804	39.0	37.0	39.0	33.0	39.0
10	36.297872120506334	38.0	35.0	39.0	32.0	39.0
11	36.45890326003818	38.0	35.0	39.0	32.0	39.0
12	35.95231909923443	37.0	35.0	39.0	32.0	39.0
13	35.497543429276256	37.0	35.0	39.0	30.0	39.0
14	36.49430130718504	38.0	35.0	40.0	31.0	41.0
15	36.62780369484775	38.0	35.0	40.0	31.0	41.0
16	36.58579392369126	38.0	35.0	40.0	31.0	41.0
17	36.52919632582466	38.0	35.0	40.0	31.0	41.0
18	36.44823629936398	38.0	35.0	40.0	31.0	41.0
19	36.50410002687413	38.0	35.0	40.0	31.0	41.0
20	36.37999324701456	38.0	35.0	40.0	31.0	41.0
21	36.497712253912255	38.0	35.0	40.0	31.0	41.0
22	36.457724932986956	38.0	35.0	40.0	31.0	41.0
23	36.389760958097035	38.0	35.0	40.0	31.0	41.0
24	35.944766780824274	38.0	34.0	40.0	30.0	41.0
25	36.196229353436095	38.0	35.0	40.0	30.0	41.0
26	36.03245222951882	38.0	34.0	40.0	30.0	41.0
27	35.967055078176145	38.0	34.0	40.0	30.0	41.0
28	36.00036521247787	38.0	35.0	40.0	30.0	41.0
29	35.45083757691857	38.0	34.0	40.0	28.0	41.0
30	35.67543636000303	38.0	34.0	40.0	29.0	41.0
31	35.50155387573129	38.0	34.0	40.0	29.0	41.0
32	35.405720054299515	38.0	34.0	40.0	29.0	41.0
33	35.47672976343878	38.0	34.0	40.0	29.0	41.0
34	35.29574286285238	38.0	34.0	40.0	28.0	41.0
35	35.28369774188436	37.0	34.0	40.0	28.0	41.0
36	35.20997994776773	37.0	34.0	40.0	27.0	41.0
37	35.06341260051957	37.0	34.0	40.0	27.0	41.0
38	35.03094314399708	37.0	34.0	40.0	27.0	41.0
39	34.93925413964898	37.0	34.0	40.0	26.0	41.0
40	34.76440349777083	37.0	33.0	40.0	26.0	41.0
41	34.884937397068654	37.0	34.0	40.0	26.0	41.0
42	34.838686337607925	37.0	34.0	40.0	27.0	41.0
43	34.83756313696846	37.0	34.0	40.0	26.0	41.0
44	34.8072298289014	37.0	34.0	40.0	26.0	41.0
45	34.590979251796774	37.0	33.0	40.0	26.0	41.0
46	34.51283756313697	36.0	33.0	40.0	26.0	41.0
47	34.43814816601319	36.0	33.0	40.0	26.0	41.0
48	34.44943529881961	36.0	33.0	40.0	26.0	41.0
49	34.347437655473705	36.0	33.0	39.0	26.0	41.0
50	34.241915367176354	36.0	33.0	39.0	25.0	41.0
51	32.991927426078924	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	5.0
11	8.0
12	11.0
13	17.0
14	29.0
15	46.0
16	95.0
17	151.0
18	295.0
19	506.0
20	782.0
21	1151.0
22	1552.0
23	1900.0
24	2250.0
25	2388.0
26	2886.0
27	3476.0
28	4316.0
29	5623.0
30	7229.0
31	9717.0
32	13422.0
33	19072.0
34	31212.0
35	31377.0
36	31859.0
37	42147.0
38	49044.0
39	27667.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.161596185252307	35.21475182778509	19.544724746935316	26.078927240027287
2	22.838872389247594	35.42457673251976	18.467692477312035	23.26885840092061
3	20.517016834227988	32.063588315957034	23.080739520813665	24.338655329001316
4	21.321517905747616	32.38780052507907	19.493732816063837	26.796948753109472
5	17.617367576022765	36.638046871231595	18.267170154560677	27.477415398184963
6	20.472226624678715	36.407549562089564	21.1175501822617	22.00267363097002
7	75.2740816284342	10.656969012065794	7.967489198668696	6.101460160831306
8	77.10014401775071	7.206744716477973	6.787783987155546	8.905327278615776
9	72.85747755321421	10.173579289007105	11.124854431818964	5.8440887259597165
10	40.80870446041579	28.695020017778265	13.980402560621826	16.515872961184115
11	33.44898395132338	25.753681410684877	22.884696219017233	17.91263841897451
12	32.28788390377685	24.914726331819654	27.059143749009447	15.73824601539405
13	24.044073566196484	33.51479110535347	22.722073304346026	19.719062024104023
14	16.246787163815025	34.8267997050737	29.497109308783703	19.429303822327576
15	15.117729343099896	30.22202162333501	32.583154746728596	22.077094286836502
16	16.455578448329327	27.553558754418727	34.055374480605835	21.935488316646108
17	16.069004485911755	27.040883125116284	28.55823760861626	28.331874780355705
18	21.006952818682343	26.90789065676229	26.882739231400006	25.202417293155367
19	22.374776910302437	31.85617519173655	26.06445655694214	19.704591341018872
20	24.623245429675926	30.55243555377926	24.178444194844303	20.645874821700513
21	20.232771273626835	30.811874229091586	24.963995562323856	23.991358934957724
22	19.07029306578648	29.57910984626622	25.484595613315786	25.866001474631517
23	20.01261016668849	30.720915649699215	23.169975399838755	26.09649878377354
24	17.87715079140855	31.213263414667757	27.820577311347083	23.08900848257661
25	18.764341480557604	28.557548528469347	26.490308087733684	26.187801903239365
26	21.588536462675975	33.57474107813479	22.881939898429586	21.954782560759643
27	18.320918406019803	30.823933131662546	26.492030788100962	24.36311767421669
28	18.542457673251974	29.218031849284394	27.587668221690866	24.651842255772767
29	18.18275783656397	33.88000358321676	26.19159184404738	21.745646736171885
30	25.011542092460775	28.129974297310522	23.807719075805707	23.050764534423
31	24.12676318382591	30.83426933386622	20.77335464887921	24.26561283342866
32	25.49010825449108	30.908689989732707	21.471048297627497	22.13015345814872
33	25.848429930885263	27.045706686144666	22.008186272145313	25.097677110824762
34	17.797562034440226	30.48869564018991	25.233081359692942	26.48066096567692
35	21.724285251617616	29.53569779701077	24.46131159515163	24.27870535621998
36	24.21668814299791	29.117426147835257	26.384878825256163	20.281006883910667
37	21.30119004141372	32.64999552097905	23.432170395738726	22.61664404186851
38	23.564818324019264	29.59530322971865	24.02236754156876	22.817510904693325
39	22.095354910729668	28.78460043687681	26.814864836929182	22.30517981546434
40	24.845129236981553	27.755803777537363	23.982056352974414	23.417010632506667
41	19.928542388765237	24.61187560725188	29.612874773464902	25.84670723051798
42	23.81529895742174	28.08759586827544	23.1696308597653	24.927474314537523
43	22.138766959985116	25.80777420221746	26.8186547777372	25.234804060060224
44	22.90640224364496	25.698210458858473	25.175198627352348	26.220188670144225
45	24.957793841001646	28.090696728936543	23.860433707044464	23.091075723017344
46	19.451354387028754	27.673803240054852	29.997381495441733	22.877460877474658
47	21.95305986039236	27.115648321056224	24.743145375238594	26.18814644331282
48	21.53616637151067	23.779122249708866	32.640692938995734	22.044018439784733
49	22.490542374983637	23.350169858256216	28.49518677517382	25.66410099158633
50	20.126652931002408	25.342989643125392	26.576098566024214	27.95425885984799
51	19.19088209149606	24.642195133716	30.614108226927872	25.552814547860063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3075.0
1	2440.5
2	1806.0
3	1202.5
4	599.0
5	727.5
6	856.0
7	768.0
8	680.0
9	643.5
10	607.0
11	607.0
12	607.0
13	610.5
14	614.0
15	648.5
16	683.0
17	791.5
18	900.0
19	865.0
20	830.0
21	838.0
22	846.0
23	1062.0
24	1278.0
25	1384.0
26	1728.5
27	1967.0
28	2631.0
29	3295.0
30	3646.0
31	3997.0
32	4219.5
33	4442.0
34	4960.5
35	5479.0
36	6566.5
37	7654.0
38	7945.0
39	8236.0
40	9501.0
41	10766.0
42	14202.0
43	17638.0
44	23543.5
45	29449.0
46	29575.5
47	29702.0
48	30297.5
49	30893.0
50	28554.5
51	26216.0
52	23795.0
53	21374.0
54	18505.0
55	15636.0
56	14797.0
57	13958.0
58	12396.0
59	10834.0
60	10159.5
61	9485.0
62	8441.0
63	7397.0
64	6124.5
65	4852.0
66	4282.0
67	3712.0
68	3124.5
69	2537.0
70	2305.0
71	2073.0
72	1787.0
73	1501.0
74	1306.5
75	878.0
76	644.0
77	406.0
78	168.0
79	125.0
80	82.0
81	80.0
82	78.0
83	105.0
84	132.0
85	80.5
86	29.0
87	19.5
88	10.0
89	7.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	2.5
96	3.0
97	3.0
98	3.0
99	5.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	290242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.7287504909696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29725726543747	27.08326155415136
2	8.555084529342663	5.771046230387056
3	2.788702180908116	2.8217832016041786
4	1.3351039378926401	1.8012555040276732
5	0.7528474385821543	1.2696301706851523
6	0.5434393993564534	1.0997719144713722
7	0.40451504162623214	0.9550650836198759
8	0.32177332856632107	0.8682409851089781
9	0.28193472598191943	0.855837542464564
>10	3.919505592726901	33.13614156462538
>50	0.7344603912355074	15.665203519821391
>100	0.05618264467031003	3.584939464309094
>500	0.004086010521477093	0.8944260306916297
>1k	0.005107513151846366	4.1933972340322905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	3019	1.040166481763494	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2659	0.9161320553193542	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC	2392	0.8241398557066173	No Hit
AATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	2060	0.709752551319244	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGT	2041	0.703206289923581	No Hit
AAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	822	0.28321194038078573	TruSeq Adapter, Index 15 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	693	0.23876627090496896	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	570	0.1963878418698879	No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	511	0.1760599775359872	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	478	0.16469015511194107	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	455	0.15676573342245437	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	441	0.15194217239407115	No Hit
AACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTG	425	0.14642953121877605	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	417	0.1436732106311285	TruSeq Adapter, Index 15 (95% over 23bp)
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	367	0.12644620695833134	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT	365	0.12575712681141943	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	321	0.11059736357935791	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	309	0.1064628826978866	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTATC	298	0.10267294188987121	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3113195195733216	0.0
2	0.0	0.0	0.0	1.7071960639742008	0.0
3	0.0	0.0	0.0	2.257426561283343	0.0
4	0.0	0.0	0.0	4.893847203368224	0.0
5	0.0	0.0	0.0	5.270774043729026	0.0
6	0.0	0.0	0.0	7.0365419201907375	0.0
7	0.0	0.0	0.0	8.454668862535401	0.0
8	0.0	0.0	0.0	9.25916993405503	0.0
9	0.0	0.0	0.0	11.157930278870735	0.0
10	0.0	0.0	0.0	12.788638446537718	0.0
11	0.0	0.0	0.0	15.39852950296649	0.0
12	0.0	0.0	0.0	16.332922182179008	0.0
13	0.0	0.0	0.0	16.754639232089083	0.0
14	0.0	0.0	0.0	17.25628957904094	0.0
15	0.0	0.0	0.0	17.592216150660484	0.0
16	0.0	0.0	0.0	18.196194899428754	0.0
17	0.0	0.0	0.0	18.87769516472461	0.0
18	0.0	0.0	0.0	19.958517375155903	0.0
19	0.0	0.0	0.0	20.404352230207895	0.0
20	0.0	0.0	0.0	20.782312690789066	0.0
21	0.0	0.0	0.0	21.25743345208481	0.0
22	0.0	0.0	0.0	21.72221801117688	0.0
23	0.0	0.0	0.0	22.202506873574464	0.0
24	0.0	0.0	0.0	22.516727420566287	0.0
25	0.0	0.0	0.0	22.787191378229203	0.0
26	0.0	0.0	0.0	23.035604771190936	0.0
27	0.0	0.0	0.0	23.30469056856003	0.0
28	0.0	0.0	0.0	23.579978087251327	0.0
29	0.0	0.0	0.0	23.869736289027777	0.0
30	0.0	0.0	0.0	24.27732719592616	0.0
31	0.0	0.0	0.0	24.566396317555697	0.0
32	0.0	0.0	0.0	24.84616285720192	0.0
33	0.0	0.0	0.0	25.116971354938293	0.0
34	0.0	0.0	0.0	25.3984605949518	0.0
35	0.0	0.0	0.0	25.69821045885847	0.0
36	0.0	0.0	0.0	25.973842517623225	0.0
37	0.0	0.0	0.0	26.218465969776943	0.0
38	0.0	0.0	0.0	26.47032476347324	0.0
39	0.0	0.0	0.0	26.75388124392748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	75	0.0	45.000004	5
TACCAGA	25	3.883576E-5	45.000004	19
TAGACTG	25	3.883576E-5	45.000004	1
TTAGCCG	25	3.883576E-5	45.000004	23
GCGCGCC	35	1.2077362E-7	45.000004	16
CGCGCCT	35	1.2077362E-7	45.000004	17
ACCCTTG	25	3.883576E-5	45.000004	28
TACTGCG	25	3.883576E-5	45.000004	1
ACGTGGT	35	1.2077362E-7	45.000004	14
ATTGGGT	25	3.883576E-5	45.000004	4
GTTGGGA	35	1.2077362E-7	45.000004	4
AATACGG	35	1.2077362E-7	45.000004	2
CCCTGCG	20	7.0245634E-4	45.0	1
GTCATAC	45	3.8198777E-10	45.0	39
CGGGTAC	45	3.8198777E-10	45.0	6
CGGCGCG	20	7.0245634E-4	45.0	14
GATACGA	20	7.0245634E-4	45.0	9
GTCGCAA	20	7.0245634E-4	45.0	15
TACCCGA	20	7.0245634E-4	45.0	28
TCTCTAG	20	7.0245634E-4	45.0	18
>>END_MODULE