FastQCFastQC Report
Sat 18 Jun 2016
SRR3552884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532571
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT65761.2347649421391702No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31780.5967279480106877No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC24300.45627719121018606No Hit
AATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT23310.4376881204571785No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGT21650.40651856747738796No Hit
AAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT11490.21574588176975465No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC11090.2082351461119738No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.15096578672139488No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7540.14157736714916883No Hit
ACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG6720.12618035905071812No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTT6690.12561705387638455No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA6520.1224249912218277No Hit
AACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT6330.11885739178438179No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6110.11472648717260235No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6060.11378764521537973No Hit
AGCCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT5690.10684021473193245No Hit
TACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT5690.10684021473193245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG207.029872E-445.0000041
TAGCGCG207.029872E-445.0000041
ATTAACG207.029872E-445.0000041
TTGTACG207.029872E-445.0000041
CCTGTCG207.029872E-445.00000413
CGACACG302.1632986E-645.0000041
TAGCACG302.1632986E-645.0000041
ATTCACG207.029872E-445.0000041
CGGTGAC207.029872E-445.00000427
TACTCGC302.1632986E-645.00000445
CGAGACG207.029872E-445.0000041
GCGGAGT207.029872E-445.00000425
CAGTCCC207.029872E-445.00000431
AGGATCC207.029872E-445.00000414
CTCTACT406.8030204E-945.00000423
GCCGTAG207.029872E-445.0000041
GTTATGA207.029872E-445.00000410
AGTCGGT207.029872E-445.00000430
AACGTGT253.8879767E-545.043
CGGGTCA253.8879767E-545.06