##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	532571
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99819179039039	31.0	31.0	33.0	28.0	34.0
2	31.295200076609504	31.0	31.0	34.0	28.0	34.0
3	31.517940330960567	31.0	31.0	34.0	30.0	34.0
4	35.476593731164485	37.0	35.0	37.0	33.0	37.0
5	34.774798102037096	35.0	35.0	37.0	32.0	37.0
6	34.8733934067007	35.0	35.0	37.0	32.0	37.0
7	35.020870456709055	35.0	35.0	37.0	32.0	37.0
8	35.34428648950093	37.0	35.0	37.0	33.0	37.0
9	36.8730028484465	39.0	37.0	39.0	33.0	39.0
10	36.033090423624266	37.0	35.0	39.0	31.0	39.0
11	36.35049974557383	38.0	35.0	39.0	32.0	39.0
12	36.129276659825635	37.0	35.0	39.0	32.0	39.0
13	35.85019837730556	37.0	35.0	39.0	30.0	39.0
14	36.84743630426741	39.0	35.0	40.0	31.0	41.0
15	36.82505994505897	38.0	35.0	40.0	32.0	41.0
16	36.71432353620456	38.0	35.0	40.0	31.0	41.0
17	36.69969825619495	38.0	35.0	40.0	32.0	41.0
18	36.60216384294301	38.0	35.0	40.0	31.0	41.0
19	36.76251992692054	38.0	35.0	40.0	31.0	41.0
20	36.59750155378344	38.0	35.0	40.0	31.0	41.0
21	36.69849466080579	38.0	35.0	40.0	31.0	41.0
22	36.69816043306902	38.0	35.0	40.0	31.0	41.0
23	36.60329984171125	38.0	35.0	40.0	31.0	41.0
24	36.13169323902353	38.0	34.0	40.0	30.0	41.0
25	36.334269045817365	38.0	35.0	40.0	30.0	41.0
26	36.20859753910746	38.0	35.0	40.0	30.0	41.0
27	35.99985917370642	38.0	34.0	40.0	30.0	41.0
28	36.10843624605921	38.0	35.0	40.0	30.0	41.0
29	35.83641429968962	38.0	34.0	40.0	29.0	41.0
30	35.729513999072424	38.0	34.0	40.0	29.0	41.0
31	35.747288155006565	38.0	34.0	40.0	29.0	41.0
32	35.660514748268305	38.0	34.0	40.0	29.0	41.0
33	35.51627858069628	38.0	34.0	40.0	28.0	41.0
34	35.62105334312233	38.0	34.0	40.0	29.0	41.0
35	35.495088917721766	38.0	34.0	40.0	28.0	41.0
36	35.43962964562471	38.0	34.0	40.0	28.0	41.0
37	35.31522182018923	38.0	34.0	40.0	27.0	41.0
38	35.353509672888684	38.0	34.0	40.0	28.0	41.0
39	35.3450356853828	38.0	34.0	40.0	28.0	41.0
40	35.08734422264825	38.0	34.0	40.0	26.0	41.0
41	35.26831915369031	38.0	34.0	40.0	27.0	41.0
42	35.19940439866234	38.0	34.0	40.0	27.0	41.0
43	35.18559215578768	38.0	34.0	40.0	27.0	41.0
44	35.057597954075604	38.0	34.0	40.0	26.0	41.0
45	34.92904983560877	37.0	33.0	40.0	26.0	41.0
46	34.87761631782429	37.0	33.0	40.0	26.0	41.0
47	34.78320261523815	37.0	33.0	40.0	26.0	41.0
48	34.84348565731142	37.0	33.0	40.0	26.0	41.0
49	34.71867412983433	37.0	33.0	40.0	26.0	41.0
50	34.64820277484129	37.0	33.0	40.0	26.0	41.0
51	33.34617168415103	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	14.0
12	10.0
13	21.0
14	24.0
15	33.0
16	84.0
17	181.0
18	353.0
19	649.0
20	1170.0
21	1782.0
22	2388.0
23	2842.0
24	3495.0
25	4166.0
26	4757.0
27	5928.0
28	7463.0
29	9717.0
30	12993.0
31	17172.0
32	23482.0
33	32969.0
34	50793.0
35	58145.0
36	65988.0
37	85109.0
38	92764.0
39	48072.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.046420101732917	34.29214132951287	21.114931154719276	26.546507414034938
2	21.258573974174336	33.714565757429526	21.9332258046345	23.09363446376164
3	21.031749757309353	31.178190325796933	22.11442230237846	25.675637614515246
4	19.97874461808848	31.32371082916644	20.318605406603062	28.378939146142017
5	16.71176237534526	35.78996978806582	19.607714276594105	27.890553559994817
6	19.964849757121588	36.42932116093441	20.918900954051196	22.68692812789281
7	78.62857722256751	7.245794457452622	8.183885341109448	5.941742978870423
8	80.73158320674614	5.522831697557697	6.152419114071176	7.593165981624986
9	74.1549952964018	9.390485024531941	11.344215137512181	5.1103045415540835
10	31.88983252937167	39.7214643681312	12.741024201467974	15.647678901029158
11	24.37797026124216	24.64197261961316	33.22768231841388	17.752374800730795
12	26.574297135968727	23.41791047578633	32.60729555308119	17.40049683516376
13	22.32153083814177	30.973335010730963	27.18210341907464	19.523030732052625
14	16.345426243637	33.06394828107426	31.43374310655293	19.15688236873581
15	14.316025468904616	31.449891188217162	32.6600584710771	21.574024871801132
16	16.116724342857573	29.89122577083619	31.9401544582788	22.05189542802744
17	15.91149349100871	29.29149352856239	29.336182405726184	25.460830574702715
18	17.95647904223099	28.934545816426354	28.797099353888967	24.31187578745369
19	19.51852429065796	33.29490340255102	28.488220349962727	18.69835195682829
20	21.651197680684827	31.069660195542003	26.43872835734578	20.84041376642739
21	18.342155318258037	32.39830933340343	26.79792929017915	22.46160605815938
22	17.887004737396516	29.648629009089866	27.63349863210727	24.830867621406348
23	19.905139408642228	30.955121476760844	23.504471704242253	25.63526741035468
24	15.758462251981426	32.56974187479228	28.61120864635889	23.060587226867405
25	16.674208697056354	30.581274609394804	27.27636315157979	25.468153541969052
26	20.891111232117407	32.56410882304894	24.426602274626294	22.118177670207352
27	15.718467584603744	32.332590396397855	26.888996960029743	25.059945058968662
28	17.781478901404697	28.22891220137784	30.436880716373967	23.552728180843495
29	19.940815403016686	29.574460494469285	28.58097793533632	21.903746167177708
30	18.28995570543646	32.799194849137486	26.161206674790776	22.749642770635276
31	22.85441753306132	30.406462236959953	23.70444504113067	23.03467518884806
32	23.694117779601218	29.436638495149005	23.958495674755103	22.910748050494675
33	20.007848718762382	30.855604229295245	24.940336593618504	24.196210458323865
34	17.68778247407388	26.834731894902276	26.46482816375657	29.012657467267278
35	21.763107641985762	28.264212658969413	26.13191480572543	23.84076489331939
36	22.654068659390013	28.05766742838044	28.703778463341038	20.584485448888504
37	18.868845656259918	31.720465440288713	26.84768791391195	22.56300098953942
38	20.327618289392397	29.602813521577403	30.431247664630632	19.638320524399564
39	19.110503576049016	28.263461585403636	29.39345176511676	23.232583073430586
40	22.02504454805087	29.12700841765699	24.16804519960719	24.67990183468495
41	20.151679306608884	23.921693070031978	28.861316143762995	27.06531147959615
42	24.957799054022843	26.494495569604805	23.557422390629608	24.990282985742745
43	20.45511302718323	26.383148913478205	27.828589990818127	25.33314806852044
44	20.656025206028865	26.26541813204249	26.829286611550383	26.24927005037826
45	23.140388793231324	28.323359702274438	26.80074581605082	21.735505688443418
46	18.513212322863996	27.770194021078883	31.7929440393863	21.92364961667083
47	20.11412562831998	27.42207142334074	26.33151260583096	26.132290342508323
48	21.172576050892744	23.665576984101648	32.76070232889136	22.401144636114246
49	21.50436279857521	23.463538194907347	30.18658545057842	24.845513555939018
50	19.372628250505567	24.391865122209058	27.381137914005834	28.854368713279545
51	19.127402731279023	24.130867058101174	32.08830371912853	24.653426491491278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3644.0
1	2877.0
2	2110.0
3	1437.5
4	765.0
5	1141.5
6	1518.0
7	1384.5
8	1251.0
9	1240.0
10	1229.0
11	1251.0
12	1273.0
13	1459.0
14	1645.0
15	1652.0
16	1659.0
17	1738.5
18	1818.0
19	1825.0
20	1832.0
21	1804.5
22	1777.0
23	2220.0
24	2663.0
25	2746.0
26	3539.0
27	4249.0
28	4936.5
29	5624.0
30	6713.5
31	7803.0
32	9539.5
33	11276.0
34	12359.0
35	13442.0
36	15605.0
37	17768.0
38	19150.5
39	20533.0
40	22766.0
41	24999.0
42	30243.5
43	35488.0
44	43375.0
45	51262.0
46	56660.0
47	62058.0
48	66537.0
49	71016.0
50	64531.0
51	58046.0
52	49762.5
53	41479.0
54	33954.5
55	26430.0
56	22213.5
57	17997.0
58	15127.5
59	12258.0
60	10505.5
61	8753.0
62	7338.0
63	5923.0
64	5076.0
65	4229.0
66	3164.5
67	2100.0
68	1688.0
69	1276.0
70	1184.0
71	1092.0
72	855.5
73	619.0
74	482.0
75	242.5
76	140.0
77	154.5
78	169.0
79	106.0
80	43.0
81	40.0
82	37.0
83	39.0
84	41.0
85	47.5
86	54.0
87	30.5
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	532571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.85221290812716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86521066558251	30.252357429153854
2	9.802615494005405	7.617066084592082
3	3.503695236055424	4.083789398292485
4	1.7338492019241505	2.694555133749738
5	1.0247738222057787	1.9907365361507081
6	0.6959095628358823	1.6222575900060838
7	0.5526713471714346	1.5030753393968226
8	0.46541455332784604	1.4465908253147504
9	0.3721046802073649	1.3011381233574402
>10	3.5510584302477706	29.032595309933956
>50	0.36679914207440273	9.175584023552727
>100	0.05620700195587707	4.199420451646829
>500	0.0062990605640207056	1.5156468398354157
>1k	0.0029072587218557104	2.327217269164751
>5k	4.845431203092851E-4	1.2379696458524028
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	6576	1.2347649421391702	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3178	0.5967279480106877	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC	2430	0.45627719121018606	No Hit
AATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	2331	0.4376881204571785	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGT	2165	0.40651856747738796	No Hit
AAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	1149	0.21574588176975465	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1109	0.2082351461119738	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	804	0.15096578672139488	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	754	0.14157736714916883	No Hit
ACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	672	0.12618035905071812	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTT	669	0.12561705387638455	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	652	0.1224249912218277	No Hit
AACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	633	0.11885739178438179	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	611	0.11472648717260235	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	606	0.11378764521537973	No Hit
AGCCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	569	0.10684021473193245	No Hit
TACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	569	0.10684021473193245	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7553678288904205E-4	0.0	0.0	1.510033403996838	0.0
2	5.63305174333563E-4	0.0	0.0	2.038225889130276	0.0
3	5.63305174333563E-4	0.0	0.0	2.6907210493999862	0.0
4	5.63305174333563E-4	0.0	0.0	5.682247061894095	0.0
5	5.63305174333563E-4	0.0	0.0	6.082944809236703	0.0
6	5.63305174333563E-4	0.0	0.0	8.178064520974669	0.0
7	5.63305174333563E-4	0.0	0.0	9.74593058953642	0.0
8	5.63305174333563E-4	0.0	0.0	10.821092398947746	0.0
9	5.63305174333563E-4	0.0	0.0	12.871523233521915	0.0
10	5.63305174333563E-4	0.0	0.0	15.210178548963425	0.0
11	7.510735657780841E-4	0.0	0.0	18.158142294642406	0.0
12	7.510735657780841E-4	0.0	0.0	19.456372953089822	0.0
13	7.510735657780841E-4	0.0	0.0	19.964474220338698	0.0
14	7.510735657780841E-4	0.0	0.0	20.49867529399836	0.0
15	7.510735657780841E-4	0.0	0.0	20.923219627054422	0.0
16	7.510735657780841E-4	0.0	0.0	21.749400549410314	0.0
17	7.510735657780841E-4	0.0	0.0	23.00388117265116	0.0
18	7.510735657780841E-4	0.0	0.0	24.4127074136594	0.0
19	7.510735657780841E-4	0.0	0.0	25.083603876290674	0.0
20	7.510735657780841E-4	0.0	0.0	25.76914627345462	0.0
21	7.510735657780841E-4	0.0	0.0	26.499189779390917	0.0
22	7.510735657780841E-4	0.0	0.0	27.336073500059147	0.0
23	7.510735657780841E-4	0.0	0.0	28.13615461600425	0.0
24	7.510735657780841E-4	0.0	0.0	28.70847267312715	0.0
25	7.510735657780841E-4	0.0	0.0	29.182963398307457	0.0
26	7.510735657780841E-4	0.0	0.0	29.61971267680741	0.0
27	7.510735657780841E-4	0.0	0.0	30.134385837756845	0.0
28	7.510735657780841E-4	0.0	0.0	30.614134077897596	0.0
29	7.510735657780841E-4	0.0	0.0	31.139134500376475	0.0
30	7.510735657780841E-4	0.0	0.0	31.7997037014783	0.0
31	7.510735657780841E-4	0.0	0.0	32.288652592799835	0.0
32	7.510735657780841E-4	0.0	0.0	32.718078904033455	0.0
33	7.510735657780841E-4	0.0	0.0	33.20777886892076	0.0
34	7.510735657780841E-4	0.0	0.0	33.66912580669995	0.0
35	7.510735657780841E-4	0.0	0.0	34.181733515343495	0.0
36	7.510735657780841E-4	0.0	0.0	34.63331649676757	0.0
37	7.510735657780841E-4	0.0	0.0	35.08846707762908	0.0
38	7.510735657780841E-4	0.0	0.0	35.53629469122427	0.0
39	7.510735657780841E-4	0.0	0.0	35.963655550152	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	20	7.029872E-4	45.000004	1
TAGCGCG	20	7.029872E-4	45.000004	1
ATTAACG	20	7.029872E-4	45.000004	1
TTGTACG	20	7.029872E-4	45.000004	1
CCTGTCG	20	7.029872E-4	45.000004	13
CGACACG	30	2.1632986E-6	45.000004	1
TAGCACG	30	2.1632986E-6	45.000004	1
ATTCACG	20	7.029872E-4	45.000004	1
CGGTGAC	20	7.029872E-4	45.000004	27
TACTCGC	30	2.1632986E-6	45.000004	45
CGAGACG	20	7.029872E-4	45.000004	1
GCGGAGT	20	7.029872E-4	45.000004	25
CAGTCCC	20	7.029872E-4	45.000004	31
AGGATCC	20	7.029872E-4	45.000004	14
CTCTACT	40	6.8030204E-9	45.000004	23
GCCGTAG	20	7.029872E-4	45.000004	1
GTTATGA	20	7.029872E-4	45.000004	10
AGTCGGT	20	7.029872E-4	45.000004	30
AACGTGT	25	3.8879767E-5	45.0	43
CGGGTCA	25	3.8879767E-5	45.0	6
>>END_MODULE